LOCUS       Exported               11080 bp DNA     circular SYN 16-FEB-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 22, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1999
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1517..1533
                     /label=6F
                     /note="color: black; sequence: atacatgttgatgcggg"
     CDS             2021..2041
                     /codon_start=1
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(2025..2040)
                     /label=ADAR-3'-CAS840-2R
                     /note="color: black; sequence: 
                     tcggagccgctagatcctccggaagatcctccgctgagctccaccttccgcttcttct"
     CDS             2045..6148
                     /codon_start=1
                     /label=Cas9-SpG(H840A) (rice codon optimized)
                     /note="color: #ff6600"
                     /translation="KDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     2054..2070
                     /label=ePPE-spG-7F
                     /note="color: black; sequence: AAGTACTCGATCGGCCT"
     primer_bind     2635..2651
                     /label=ePPE-spG-8F
                     /note="color: black; sequence: GGAGAACCCGATCAACG"
     primer_bind     3102..3118
                     /label=9F
                     /note="color: black; sequence: atcagtcgaagaacggc"
     primer_bind     3672..3689
                     /label=ePPE-spG-10F
                     /note="color: black; sequence: AGCAGAAGAAGGCTATCG"
     primer_bind     4206..4222
                     /label=ePPE-spG-11F
                     /note="color: black; sequence: ACGAGCATATCGCGAAC"
     primer_bind     4737..4753
                     /label=ePPE-spG-12F
                     /note="color: black; sequence: ACAACCTCACCAAGGCT"
     primer_bind     5294..5310
                     /label=ePPE-spG-13F
                     /note="color: black; sequence: CGCAAGGTTCTCTCCAT"
     misc_feature    5447..5449
                     /label=D1135L
                     /note="color: #33cccc"
     misc_feature    5450..5452
                     /label=S1136W
                     /note="color: #33cccc"
     misc_feature    5696..5698
                     /label=G1218K
                     /note="color: #33cccc"
     misc_feature    5699..5701
                     /label=E1219Q
                     /note="color: #33cccc"
     primer_bind     5838..5854
                     /label=ePPE-spG-14F
                     /note="color: black; sequence: ACCTCGACGAGATCATC"
     misc_feature    6047..6049
                     /label=R1335Q
                     /note="color: #33cccc"
     misc_feature    6053..6055
                     /label=T1337R
                     /note="color: #33cccc"
     CDS             6149..6196
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6149..6196
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    6203..6250
                     /label=XTEN
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    6251..6418
                     /label=NC
                     /note="color: #ff00ff"
     CDS             6419..6439
                     /codon_start=1
                     /label=NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(6423..6480)
                     /label=ADAR-3'-CAS840-2R
                     /note="color: black; sequence: 
                     tcggagccgctagatcctccggaagatcctccgctgagctccaccttccgcttcttct"
     primer_bind     6426..6480
                     /label=ADAR-3-aa-1F
                     /note="color: black; sequence: 
                     agaagcggaaggtggagctcagcggaggatcttccggaggatctagcggctccga"
     CDS             6446..6541
                     /codon_start=1
                     /label=32aa linker
                     /note="color: #ffcc00"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     complement(6513..6547)
                     /label=SacI-NotI-StuI-32aa-Re
                     /note="color: black; sequence: 
                     taccgagctcGCGGCCGCAAGCTGaggcctagatcctccggaagagcctccgctagatt
                     "
     CDS             6548..8089
                     /codon_start=1
                     /label=LHB(rice)
                     /note="color: #008000"
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     misc_feature    6548..6616
                     /label=the first 23aa
                     /note="color: #ff99cc; direction: RIGHT"
     misc_feature    6617..6919
                     /label=24-124 aa Finger
                     /note="color: #3366ff; direction: RIGHT"
     misc_feature    6920..7012
                     /label=125-155aa PLAM
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    7013..7132
                     /label=156-195aa Finger
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    7133..7372
                     /label=196-275 aa PLAM
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    7373..7561
                     /label=276-338aa Thumb
                     /note="color: #99ccff; direction: RIGHT"
     misc_feature    7562..7950
                     /label=339-468aa connection
                     /note="color: #999999; direction: RIGHT"
     CDS             8102..8122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8180..8356
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8910..8926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8934..8950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8958..8988)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8958 .. 8964 / #ffffff / -10
                        2: 8965 .. 8982 / #ffffff
                        3: 8983 .. 8988 / #ffffff / -35"
     protein_bind    9003..9024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(9312..9900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10071..10931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10071 .. 10862 / #ccffcc
                        2: 10863 .. 10931 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10932..11036)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg ccaaagaaga agaggaaggt
     2041 tcttaaggac aagaagtact cgatcggcct cgacatcggg acgaactcag ttggctgggc
     2101 cgtgatcacc gacgagtaca aggtgccctc taagaagttc aaggtcctgg ggaacaccga
     2161 ccgccattcc atcaagaaga acctcatcgg cgctctcctg ttcgacagcg gggagaccgc
     2221 tgaggctacg aggctcaaga gaaccgctag gcgccggtac acgagaagga agaacaggat
     2281 ctgctacctc caagagattt tctccaacga gatggccaag gttgacgatt cattcttcca
     2341 ccgcctggag gagtctttcc tcgtggagga ggataagaag cacgagcggc atcccatctt
     2401 cggcaacatc gtggacgagg ttgcctacca cgagaagtac cctacgatct accatctgcg
     2461 gaagaagctc gtggactcca ccgataaggc ggacctcaga ctgatctacc tcgctctggc
     2521 ccacatgatc aagttccgcg gccatttcct gatcgagggg gatctcaacc cagacaacag
     2581 cgatgttgac aagctgttca tccaactcgt gcagacctac aaccaactct tcgaggagaa
     2641 cccgatcaac gcctctggcg tggacgcgaa ggctatcctg tccgcgaggc tctcgaagtc
     2701 caggaggctg gagaacctga tcgctcagct cccaggcgag aagaagaacg gcctgttcgg
     2761 gaacctcatc gctctcagcc tggggctcac cccgaacttc aagtcgaact tcgatctcgc
     2821 tgaggacgcc aagctgcaac tctccaagga cacctacgac gatgacctcg ataacctcct
     2881 ggcccagatc ggcgatcaat acgcggacct gttcctcgct gccaagaacc tgtcggacgc
     2941 catcctcctg tcagatatcc tccgcgtgaa caccgagatc acgaaggctc cactctctgc
     3001 ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga aggcgctggt
     3061 ccgccaacag ctcccggaga agtacaagga gattttcttc gatcagtcga agaacggcta
     3121 cgctgggtac atcgacggcg gggcctcaca agaggagttc tacaagttca tcaagccaat
     3181 cctggagaag atggacggca cggaggagct cctggtgaag ctcaacaggg aggacctcct
     3241 gcggaagcag agaaccttcg ataacggcag catcccccac caaatccatc tcggggagct
     3301 gcacgccatc ctgagaaggc aagaggactt ctaccctttc ctcaaggata accgggagaa
     3361 gatcgagaag atcctgacct tcagaatccc atactacgtc ggccctctcg cgcgggggaa
     3421 ctcaagattc gcttggatga cccgcaagtc tgaggagacc atcacgccgt ggaacttcga
     3481 ggaggtggtg gacaagggcg ctagcgctca gtcgttcatc gagaggatga ccaacttcga
     3541 caagaacctg cccaacgaga aggtgctccc taagcactcg ctcctgtacg agtacttcac
     3601 cgtctacaac gagctcacga aggtgaagta cgtcaccgag ggcatgcgca agccagcgtt
     3661 cctgtccggg gagcagaaga aggctatcgt ggacctcctg ttcaagacca accggaaggt
     3721 cacggttaag caactcaagg aggactactt caagaagatc gagtgcttcg attcggtcga
     3781 gatcagcggc gttgaggacc gcttcaacgc cagcctcggg acctaccacg atctcctgaa
     3841 gatcatcaag gataaggact tcctggacaa cgaggagaac gaggatatcc tggaggacat
     3901 cgtgctgacc ctcacgctgt tcgaggacag ggagatgatc gaggagcgcc tgaagacgta
     3961 cgcccatctc ttcgatgaca aggtcatgaa gcaactcaag cgccggagat acaccggctg
     4021 ggggaggctg tcccgcaagc tcatcaacgg catccgggac aagcagtccg ggaagaccat
     4081 cctcgacttc ctcaagagcg atggcttcgc caacaggaac ttcatgcaac tgatccacga
     4141 tgacagcctc accttcaagg aggatatcca aaaggctcaa gtgagcggcc agggggactc
     4201 gctgcacgag catatcgcga acctcgctgg ctcccccgcg atcaagaagg gcatcctcca
     4261 gaccgtgaag gttgtggacg agctcgtgaa ggtcatgggc cggcacaagc ctgagaacat
     4321 cgtcatcgag atggccagag agaaccaaac cacgcagaag gggcaaaaga actctaggga
     4381 gcgcatgaag cgcatcgagg agggcatcaa ggagctgggg tcccaaatcc tcaaggagca
     4441 cccagtggag aacacccaac tgcagaacga gaagctctac ctgtactacc tccagaacgg
     4501 cagggatatg tacgtggacc aagagctgga tatcaaccgc ctcagcgatt acgacgtcga
     4561 tgccatcgtt ccccagtctt tcctgaagga tgactccatc gacaacaagg tcctcaccag
     4621 gtcggacaag aaccgcggca agtcagataa cgttccatct gaggaggtcg ttaagaagat
     4681 gaagaactac tggaggcagc tcctgaacgc caagctgatc acgcaaagga agttcgacaa
     4741 cctcaccaag gctgagagag gcgggctctc agagctggac aaggccggct tcatcaagcg
     4801 gcagctggtc gagaccagac aaatcacgaa gcacgttgcg caaatcctcg actctcggat
     4861 gaacacgaag tacgatgaga acgacaagct gatcagggag gttaaggtga tcaccctgaa
     4921 gtctaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc gcgagatcaa
     4981 caactaccac catgcccatg acgcttacct caacgctgtg gtcggcaccg ctctgatcaa
     5041 gaagtaccca aagctggagt ccgagttcgt gtacggggac tacaaggttt acgatgtgcg
     5101 caagatgatc gccaagtcgg agcaagagat cggcaaggct accgccaagt acttcttcta
     5161 ctcaaacatc atgaacttct tcaagaccga gatcacgctg gccaacggcg agatccggaa
     5221 gagaccgctc atcgagacca acggcgagac gggggagatc gtgtgggaca agggcaggga
     5281 tttcgcgacc gtccgcaagg ttctctccat gccccaggtg aacatcgtca agaagaccga
     5341 ggtccaaacg ggcgggttct caaaggagtc tatcctgcct aagcggaaca gcgacaagct
     5401 catcgccaga aagaaggact gggacccaaa gaagtacggc gggttcctct ggcctaccgt
     5461 ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga agctcaagag
     5521 cgtgaaggag ctcctgggga tcaccatcat ggagaggtcc agcttcgaga agaacccaat
     5581 cgacttcctg gaggccaagg gctacaagga ggtgaagaag gacctgatca tcaagctccc
     5641 gaagtactct ctcttcgagc tggagaacgg caggaagaga atgctggctt ccgctaagca
     5701 gctccagaag gggaacgagc tcgcgctgcc aagcaagtac gtgaacttcc tctacctggc
     5761 ttcccactac gagaagctca agggcagccc ggaggacaac gagcaaaagc agctgttcgt
     5821 cgagcagcac aagcattacc tcgacgagat catcgagcaa atctccgagt tcagcaagcg
     5881 cgtgatcctc gccgacgcga acctggataa ggtcctctcc gcctacaaca agcaccggga
     5941 caagcccatc agagagcaag cggagaacat catccatctc ttcaccctga cgaacctcgg
     6001 cgctcctgct gctttcaagt acttcgacac cacgatcgat cggaagcaat acaggtccac
     6061 gaaggaggtc ctggacgcga ccctcatcca ccagtcgatc accggcctgt acgagacgag
     6121 gatcgacctc tcacaactcg gcggggataa gagacccgca gcaaccaaga aggcagggca
     6181 agcaaagaag aagaagacgc gttccgggag cgagacgcca ggcacctccg agtcggccac
     6241 cccagaatct gccacagtgg tgtccggcca aaagcaggac cgccagggcg gagaacgcag
     6301 aaggtcccag ctcgataggg atcagtgtgc ctactgcaag gagaagggcc actgggccaa
     6361 agactgcccg aaaaagccgc gcggcccacg cggcccaagg ccacaaacat ccctccttcc
     6421 aaagaagaag cggaaggtgg agctcagcgg aggatcttcc ggaggatcta gcggctccga
     6481 gacaccagga acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc
     6541 taggcctacc ctcaacatcg aggatgagta tcgcctccac gaaacctcca aagaaccgga
     6601 cgtgtccctc ggcagcacat ggctcagcga cttcccacaa gcgtgggccg aaaccggcgg
     6661 catgggcctc gccgtccgcc aagccccact cattatcccg ctgaaggcga cctccacacc
     6721 ggtgtccatc aagcagtacc cgatgagcca agaggcgagg ctcgggatta agccgcacat
     6781 tcagcgcctc ctcgatcaag gcattctcgt gccgtgccaa tccccgtgga atacaccact
     6841 cctcccggtc aaaaagccgg gcaccaacga ctatcgcccg gtccaagatc tccgcgaggt
     6901 caacaagcgc gtggaagaca tccacccgac cgtcccgaac ccgtataatc tgctctccgg
     6961 gctcccacca tcccaccagt ggtatacagt gctggacctc aaagacgcct tcttctgtct
     7021 ccgcctccac ccaacaagcc agccgctctt cgccttcgag tggcgcgacc cggagatggg
     7081 catctccggc caactgacat ggacacgcct cccgcaaggc ttcaagaaca gcccgacact
     7141 cttcaacgag gcgctccata gggacctcgc ggattttcgc atccagcatc cggacctcat
     7201 cctcctccag tatgtggatg atctcctcct cgccgcgacc tccgagctgg attgtcaaca
     7261 aggcacacgc gcgctcctcc aaacactcgg gaacctcggc tatcgcgcgt ccgcgaaaaa
     7321 ggcccaaatc tgccagaagc aagtgaagta cctcgggtat ctgctcaagg aaggccaacg
     7381 ctggctcacc gaagcgcgca aagaaacagt gatggggcaa ccgacaccga aaacaccacg
     7441 ccagctgcgc gagtttctcg gcaaagccgg cttctgtcgc ctcttcatcc cgggctttgc
     7501 cgagatggcc gcgccactct acccactcac caagccgggc acactgttta actgggggcc
     7561 ggatcagcag aaagcctacc aagagatcaa acaagcgctc ctcaccgccc cagcgctcgg
     7621 gctcccagat ctcacaaagc cgttcgagct gttcgtcgat gagaagcaag gctacgcgaa
     7681 gggcgtgctc acacagaagc tcggcccgtg gaggaggcca gtggcctatc tctccaaaaa
     7741 actcgatcca gtggccgccg gctggccacc gtgtctgcgc atggtcgccg cgattgccgt
     7801 gctcacaaag gatgccggca aactcacaat gggccagccg ctggtgatcc tcgcgccaca
     7861 tgccgtggaa gccctcgtca aacagccgcc ggataggtgg ctctccaatg cgcgcatgac
     7921 ccattaccaa gcgctcctcc tcgacaccga tcgcgtccag ttcggcccag tggtcgccct
     7981 caatccggcg acactgctgc cactcccaga ggagggcctc caacacaact gtctggatat
     8041 tctcgcggaa gcgcatggca caaggccaga cctcacagat caaccgctca gcggcggcag
     8101 cccgaagaag aaaaggaagg tgtgagaatt cggtacgctg aaatcaccag tctctctcta
     8161 caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc cgataaggga
     8221 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     8281 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     8341 tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat aaaccagaca
     8401 cgagacgact aaacctggag cccagacgcc gttcgaagct agaagtaccg cttaggcagg
     8461 aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc cgtaggtttg
     8521 gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat aaggttgcag
     8581 gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata cttatactat
     8641 acgctaaggg aatgcttgta tttataccct atacccccta ataacccctt atcaatttaa
     8701 gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa taggtctatg
     8761 accaaaactc aagaggataa aacctcacca aaatacgaaa gagttcttaa ctctaaagat
     8821 aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat cgcatgcgat
     8881 atctcgagat ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9061 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9121 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9721 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10381 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10681 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11041 tttccccgaa aagtgccacc tgccagtgcc aagctaattc
//
