LOCUS       Exported               10770 bp ds-DNA     circular SYN 04-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 4, 2022 from SnapGene 5.2.2
            https://www.snapgene.com
COMMENT     Insert from pDONR223_Flag_EGFP 652 to 1440
            Backbone from EF1a_gate_PGK_hygro destination vector 1704 to 21
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     14..21
                     /locus_tag="attR1"
                     /label=attR1
     CDS             44..67
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             83..799
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     protein_bind    complement(801..825)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     811..825
                     /locus_tag="attR2"
                     /label=attR2
     CDS             872..961
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        1003..1502
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1523..2548
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_recomb     2579..2612
                     /locus_tag="LoxP"
                     /label=LoxP
     protein_bind    2579..2612
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             2668..3256
                     /locus_tag="WPRE"
                     /label=WPRE
     misc_feature    2668..3256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3139..3150
                     /locus_tag="Factor Xa site"
                     /label=Factor Xa site
     CDS             complement(3139..3150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3259..3275)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3465..3698
                     /locus_tag="3' LTR (-U3)"
                     /label=3' LTR (-U3)
     LTR             3465..3698
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             3518..3698
                     /locus_tag="5' LTR (truncated)"
                     /label=5' LTR (truncated)
     polyA_signal    3770..3891
                     /locus_tag="SV40 polyA signal"
                     /label=SV40 polyA signal
     misc_feature    3931..4066
                     /locus_tag="SV40 ori"
                     /label=SV40 ori
     rep_origin      3931..4066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4086..4105)
                     /locus_tag="T7(1)"
                     /label=T7
     promoter        complement(4087..4105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4128..4486)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(4163..4469)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     promoter        4513..4617
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
     promoter        4619..4976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4780..4976
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     misc_feature    4827..4962
                     /locus_tag="SV40 ori(1)"
                     /label=SV40 ori
     rep_origin      4827..4962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5011..5805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5014..5805
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     polyA_signal    6037..6084
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6337..7019
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      6413..7001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        7176..7479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7481..7679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             7697..7877
                     /locus_tag="5' LTR (truncated)(1)"
                     /label=5' LTR (truncated)
     LTR             7697..7877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             7924..8049
                     /locus_tag="HIV-1 AA¨"
                     /label=HIV-1 AA¨
     misc_feature    7924..8049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             8548..8781
                     /locus_tag="RRE"
                     /label=RRE
     misc_feature    8548..8781
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8966..9010
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9308..9425
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
     promoter        9497..10675
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9728..10666
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10692..10711
                     /locus_tag="T7"
                     /label=T7
     promoter        10692..10710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatctggcca atcacaagtt tgtacaaaaa agcaggctaa atggactaca aggacgacga
       61 tgacaagggt ggcggaggga gcgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagtgaa acccagcttt cttgtacaaa gtggtgggtg gaggcggttc
      841 aggtggcgga ggaagcggag gcggtggaag ttacccctac gacgtgcccg actacgccgg
      901 ctatccgtat gatgtcccgg actatgcagg atcctatcca tacgacgttc cagattacgc
      961 ttagttaatt aaggccggcc ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     1021 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     1081 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     1141 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     1201 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     1261 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     1321 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     1381 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     1441 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     1501 cgacctgcag cccaagctta ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     1561 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     1621 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     1681 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     1741 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     1801 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     1861 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     1921 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     1981 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     2041 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     2101 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     2161 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     2221 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     2281 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     2341 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     2401 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     2461 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     2521 cagcactcgt ccgagggcaa aggaatagtg tacaagtagg aattcgtcga gggacctaat
     2581 aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag
     2641 gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3061 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3241 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     3301 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     3361 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     3421 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3481 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     3721 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3901 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4081 attcgcccta tagtgagtcg tattagcatg tgagcaaaag gccagcataa atcaaaagaa
     4141 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4201 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4261 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4321 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4381 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4441 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     4501 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4561 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     4621 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4681 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4741 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4801 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4861 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4921 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4981 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5041 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5101 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5161 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     5221 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5281 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5341 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5401 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5461 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5521 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5581 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5641 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5701 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5761 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5821 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5881 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5941 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     6001 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     6061 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     6121 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     6181 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     6241 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     6301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6541 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7081 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     7141 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7201 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7261 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7321 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     7381 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     7441 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7501 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7561 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     7621 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     7681 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7861 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7921 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7981 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8041 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8101 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8161 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8221 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8281 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8341 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgcgct
     8401 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8461 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8521 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8581 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8641 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8701 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8761 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8821 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8881 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8941 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9001 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9061 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9121 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9181 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9241 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     9301 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9361 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9421 attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct
     9481 agccctagat ttaaatggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     9541 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     9601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     9661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     9721 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     9781 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     9841 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     9901 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     9961 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
    10021 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
    10081 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
    10141 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
    10201 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
    10261 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
    10321 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
    10381 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
    10441 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
    10501 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
    10561 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
    10621 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat
    10681 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctagcc gcggaatcta
    10741 gactgccgga tcgaattgtc gacgaattcg
//
