LOCUS       Exported               10983 bp ds-DNA     circular SYN 22-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 22, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    854..1714
                     /label=AmpR
                     /note="Unknown feature type:ORF pUC bla Gene Amp Resist."
     old_sequence    2319..3026
                     /label=flanking sequence
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
     misc_feature    4034..8128
                     /label=geneblock1
                     /note="geneblock1"
     misc_feature    4074..5047
                     /label=Homology left
                     /note="Homology left"
     misc_feature    4074..4085
                     /label=Untitled
     misc_feature    4087..4222
                     /label=Untitled
     misc_feature    4224..4251
                     /label=Untitled
     misc_feature    4253..4295
                     /label=Untitled
     misc_feature    4302..4339
                     /label=Untitled
     primer_bind     4363..4392
                     /label=1018.S36 - CAACTTTAGAGACCTGCAGAACCTGATAGA
     primer_bind     complement(4363..4392)
                     /label=1018.S35 - TCTATCAGGTTCTGCAGGTCTCTAAAGTTG
     primer_bind     4842..4869
                     /label=1018.S38 - GCTGACTTATTAAGGTGTGCATGCTCTT
     primer_bind     complement(4842..4869)
                     /label=1034.S37 - AAGAGCATGCACACCTTAATAAGTCAGC
     misc_feature    4851..4894
                     /label=Untitled
     misc_feature    4896..4979
                     /label=Untitled
     misc_feature    4981..4999
                     /label=Untitled
     misc_feature    5001..5046
                     /label=Untitled
     misc_structure  5008..5812
                     /label=Untitled
     primer_bind     5008..5025
                     /label=TCTTCTCTGAGCCCTTGC 1056C.C1
     primer_bind     5055..5082
                     /label=1056H.C1 - CGCTTGCTTGAATAGAATTCTCATCGGCGCGCCTTTTTG
     regulatory      5056..5475
                     /note="U6:3 promoter"
     primer_bind     5056..5082
                     /label=TTTTTGCTCACCTGTGATTGCTCCTAC
     primer_bind     complement(5440..5476)
                     /label=1056H.C9
     primer_bind     complement(5440..5475)
                     /label=CGACGTTAAATTGAAAATAGGTCTATATATACGAAC
     primer_bind     5454..5513
                     /label=1056H.C10
     primer_bind     complement(5455..5505)
                     /label=GCTCTAAAACGGGGCAAGTAGTTGACAATCCGACGTTAAATTGAAAATAG
     primer_bind     complement(5455..5475)
                     /label=CGACGTTAAATTGAAAATAGG
     primer_bind     5466..5516
                     /label=TTTAACGTCGGATTGTCAACTACTTGCCCCGTTTTAGAGCTAGAAATAGC
     misc_feature    5476..5495
                     /label=Nikolai site 1
                     /note="Nikolai site 1"
     primer_bind     complement(5477..5494)
                     /label=GGGCAAGTAGTTGACAAT 1056C.S2
     primer_bind     5477..5494
                     /label=ATTGTCAACTACTTGCCC 1056C.S1
     misc_feature    5496..5571
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     primer_bind     5496..5516
                     /label=GTTTTAGAGCTAGAAATAGCA
     primer_bind     5496..5513
                     /label=GTTTTAGAGCTAGAAATA
     3'UTR           5572..5805
                     /label=U6:3 terminator
                     /note="U6:3 terminator"
     primer_bind     5780..5829
                     /label=1056C.C5 - TTAATGTTCGCTTAATGCGTATGCATTAATTAACATACT
     primer_bind     complement(5784..5805)
                     /label=1056H.C4 - CACTGAACATTGTCAGATCCGAGATCGGCCGGCCTAGGA
     primer_bind     complement(5784..5805)
                     /label=ATGCATACGCATTAAGCGAACA
     misc_feature    5813..6353
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
     misc_structure  5813..5852
                     /label=GenPart 1056D1
                     /note="GenPart 1056D1"
     primer_bind     5813..5829
                     /label=CATACTCGGTGGCCTCC 1056D.C1
     misc_structure  5818..7326
                     /label=gBlock 1056D2-Replacement
                     /note="gBlock 1056D2-Replacement"
     primer_bind     complement(5835..5852)
                     /label=CAGTGCAAAAAAGTTGGT 1056C.C4
     primer_bind     complement(6248..6274)
                     /label=AACAGATGCTGTTCAACTGTGTTTACC
     primer_bind     complement(6300..6318)
                     /label=TGGGCGCGTTGGCGTTGTT
     primer_bind     complement(6334..6353)
                     /label=CACCAGAGACAGGTTGCGGC
     CDS             6360..7079
                     /label=mVenus
                     /note="mVenus"
     primer_bind     complement(6776..6796)
                     /label=TAGTTGTACTCCAGCTTGTGC 1056D.S4
     primer_bind     6776..6796
                     /label=GCACAAGCTGGAGTACAACTA 1056D.S3
     misc_feature    7085..7325
                     /label=Sv40
                     /note="Sv40"
     primer_bind     7085..7111
                     /label=GACTCTAGATCATAATCAGCCATACCA
     primer_bind     complement(7276..7300)
                     /label=TGGACAAACCACAACTAGAATGCAG 175A
     primer_bind     complement(7296..7355)
                     /label=1056C.C6 - GAGCAATCACAGGTGAGCAAAAACTTAAGGAAGCTTTAA
     primer_bind     complement(7296..7325)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
     regulatory      7333..7752
                     /note="U6:3 promoter"
     primer_bind     7333..7359
                     /label=1056H.C1 - CGCTTGCTTGAATAGAATTCTCATCGGCGCGCCTTTTTG
     primer_bind     7333..7359
                     /label=TTTTTGCTCACCTGTGATTGCTCCTAC
     primer_bind     complement(7717..7753)
                     /label=1056H.C9
     primer_bind     complement(7717..7752)
                     /label=CGACGTTAAATTGAAAATAGGTCTATATATACGAAC
     primer_bind     7731..7790
                     /label=1056H.C10
     primer_bind     complement(7732..7782)
                     /label=GCTCTAAAACGGGGCAAGTAGTTGACAATCCGACGTTAAATTGAAAATAG
     primer_bind     complement(7732..7752)
                     /label=CGACGTTAAATTGAAAATAGG
     primer_bind     7743..7793
                     /label=TTTAACGTCGGATTGTCAACTACTTGCCCCGTTTTAGAGCTAGAAATAGC
     misc_feature    7753..7772
                     /label=Max Scott site
                     /note="Max Scott site"
     misc_feature    7773..7848
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     primer_bind     7773..7793
                     /label=GTTTTAGAGCTAGAAATAGCA
     primer_bind     7773..7790
                     /label=GTTTTAGAGCTAGAAATA
     3'UTR           7849..8082
                     /label=U6:3 terminator
                     /note="U6:3 terminator"
     primer_bind     complement(8061..8083)
                     /label=1056H.C4 - CACTGAACATTGTCAGATCCGAGATCGGCCGGCCTAGGA
     primer_bind     complement(8061..8082)
                     /label=ATGCATACGCATTAAGCGAACA
     misc_feature    8089..9838
                     /label=geneblock 2
                     /note="geneblock 2"
     misc_feature    8089..9039
                     /label=Homology right
                     /note="Homology right"
     misc_feature    8089..8093
                     /label=Untitled
     misc_feature    8095..8147
                     /label=Untitled
     misc_feature    8150..8167
                     /label=Untitled
     misc_feature    8169..8197
                     /label=Untitled
     misc_feature    8199..8240
                     /label=Untitled
     misc_feature    8242..8250
                     /label=Untitled
     misc_difference 8251..8264
                     /label=extra sequence
                     /note="extra sequence"
     misc_feature    8265..8283
                     /label=Untitled
     misc_feature    8285..8367
                     /label=Untitled
     primer_bind     8396..8422
                     /label=1018.S40 - GTACACGTAAAGCGGCAATTAAAAGCA
     primer_bind     complement(8396..8422)
                     /label=1018.S39 - TGCTTTTAATTGCCGCTTTACGTGTAC
     misc_feature    8433..8513
                     /label=Untitled
     misc_feature    8514..8649
                     /label=Untitled
     misc_feature    8651..8716
                     /label=Untitled
     misc_feature    8718..8734
                     /label=Untitled
     misc_feature    8736..8837
                     /label=Untitled
     misc_feature    8839..9028
                     /label=Untitled
     primer_bind     complement(9010..9039)
                     /label=1018.S41 - GATGAGAATTCTATTCAAGCAAGCGTAGTG
     primer_bind     9015..9074
                     /label=1056H.C1 - CGCTTGCTTGAATAGAATTCTCATCGGCGCGCCTTTTTG
     regulatory      9048..9467
                     /note="U6:3 promoter"
     primer_bind     9048..9074
                     /label=TTTTTGCTCACCTGTGATTGCTCCTAC
     primer_bind     complement(9432..9491)
                     /label=1056H.C9
     primer_bind     complement(9432..9467)
                     /label=CGACGTTAAATTGAAAATAGGTCTATATATACGAAC
     primer_bind     9446..9505
                     /label=1056H.C10
     primer_bind     complement(9447..9497)
                     /label=GCTCTAAAACGGGGCAAGTAGTTGACAATCCGACGTTAAATTGAAAATAG
     primer_bind     complement(9447..9467)
                     /label=CGACGTTAAATTGAAAATAGG
     primer_bind     9458..9508
                     /label=TTTAACGTCGGATTGTCAACTACTTGCCCCGTTTTAGAGCTAGAAATAGC
     misc_feature    9468..9487
                     /label=site 4
                     /note="site 4"
     misc_feature    9488..9563
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     primer_bind     9488..9508
                     /label=GTTTTAGAGCTAGAAATAGCA
     primer_bind     9488..9505
                     /label=GTTTTAGAGCTAGAAATA
     3'UTR           9564..9797
                     /label=U6:3 terminator
                     /note="U6:3 terminator"
     misc_structure  9759..9797
                     /label=Untitled
     primer_bind     complement(9776..9835)
                     /label=1056H.C4 - CACTGAACATTGTCAGATCCGAGATCGGCCGGCCTAGGA
     primer_bind     complement(9776..9797)
                     /label=ATGCATACGCATTAAGCGAACA
     misc_binding    9810..10510
                     /label=piggyBacL
     old_sequence    10511..10639
                     /label=flanking seq
     misc_feature    complement(10640..10983)
                     /label=Untitled
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggttta aacacccgta
     4081 ctttaagtta tcctgatttg gaaaaaaatt ttaacatttg aagtttttat tgtttagaaa
     4141 attcaatgga gtcgccccac atatgggaca gggttggctt tatctggtgc ctgtcagtgt
     4201 gtttaatact tttccatcta ttactgtcaa gtataaacta aagtttatat agaccgtttt
     4261 cccattttca taaacaaatt tggaagactg caaaatctta ttttgcttct ccaagtttgt
     4321 atgctccttt attaagcatt cctttgtttc ctttatgaat ttcaacttta gagacctgca
     4381 gaacctgata gattgtggaa atcaattgtt ccatagtata tttccgctag aatcaatcat
     4441 tcgtaaagga gtttctgctg tggcgaatat actgccatcg acatcacact tctgtggacc
     4501 tgcaaatccc tgtttgtagt tcgaatgacc tgagcttcca tcgtatccat aactaagtag
     4561 aagttcggcg attaaaaatg ttgtcccttg atctttcctt attttctaaa caactttttt
     4621 ttgtaaagtt aaaatccggc cagctgtgtg gttaagcaaa cttagcaaag gcacttctgc
     4681 ttttgtgtca ttcgttcttt ttgtttgcaa cttcgtagta cgaggaaaaa ggttgttttt
     4741 cgtttgatct ctaattgaga tattttgttg cttggttaaa ttttgatctt aaagaacagc
     4801 tagtgcgtcg ttaaatagta ttttctccaa tttcgcgagg ggctgactta ttaaggtgtg
     4861 catgctcttg gctttttcag ggcagatagc aaacagtttt ataatacatg ccagatctga
     4921 tttactgccc ttacgcgcag ccgcactagc tgaatttatt aataatttaa tatttccagc
     4981 cgtggatttg atcaaatttt aagctgctct tctctgagcc cttgctctcg acaacaaata
     5041 aggcaatgcg gccgcttttt gctcacctgt gattgctcct actcaaatac aaaaacatca
     5101 aattttctgt caataaagca tatttattta tatttatttt acaggaaaga attcctttta
     5161 aagtgtattt taacctataa tgaaaaacga ttaaaaaaaa tacataaaat aattcgaaaa
     5221 tttttgaata gcccaggttg ataaaaattc atttcatacg ttttataact tatgccccta
     5281 agtatttttt gaccatagtg tttcaattct acattaattt tacagagtag aatgaaacgc
     5341 cacctactca gccaagaggc gaaaaggtta gctcgccaag cagagagggc gccagtgctc
     5401 actacttttt ataattctca acttcttttt ccagactcag ttcgtatata tagacctatt
     5461 ttcaatttaa cgtcggattg tcaactactt gccccgtttt agagctagaa atagcaagtt
     5521 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgcc
     5581 tacctggagc ctgagagttg ttcaataaaa taaaaatgtt tcgttttttt gctttcgcca
     5641 gtatttatta tttttcatca atatgtattc aatttggtat gtatttagta attgtaatat
     5701 atagacaatg gttttccgtt gacgtacata catctgacgt gtgtttattt agacataata
     5761 gttatgtttt cacatctttt taatgttcgc ttaatgcgta tgcattaatt aacatactcg
     5821 gtggcctccc caccaccaac ttttttgcac tgcaaaaaaa cacgcttttg cacgcgggcc
     5881 catacatagt acaaactcta cgtttcgtag actattttac ataaatagtc tacaccgttg
     5941 tatacgctcc aaatacacta ccacacattg aacctttttg cagtgcaaaa aagtacgtgt
     6001 cggcagtcac gtaggccggc cttatcgggt cgcgtcctgt cacgtacgaa tcacattatc
     6061 ggaccggacg agtgttgtct tatcgtgaca ggacgccagc ttcctgtgtt gctaaccgca
     6121 gccggacgca actccttatc ggaacaggac gcgcctccat atcagccgcg cgttatctca
     6181 tgcgcgtgac cggacacgag gcgcccgtcc cgcttatcgc gcctataaat acagcccgca
     6241 acgatctggt aaacacagtt gaacagcatc tgttacagcg acacaacatg agccggtcca
     6301 acaacgccaa cgcgcccacg ccatccaacc gccgccgcaa cctgtctctg gtggctagca
     6361 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6421 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6481 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6541 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     6601 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6661 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6721 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6781 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     6841 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     6901 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6961 acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7021 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     7081 cgctgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7141 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     7321 agcttcctta agtttttgct cacctgtgat tgctcctact caaatacaaa aacatcaaat
     7381 tttctgtcaa taaagcatat ttatttatat ttattttaca ggaaagaatt ccttttaaag
     7441 tgtattttaa cctataatga aaaacgatta aaaaaaatac ataaaataat tcgaaaattt
     7501 ttgaatagcc caggttgata aaaattcatt tcatacgttt tataacttat gcccctaagt
     7561 attttttgac catagtgttt caattctaca ttaattttac agagtagaat gaaacgccac
     7621 ctactcagcc aagaggcgaa aaggttagct cgccaagcag agagggcgcc agtgctcact
     7681 actttttata attctcaact tctttttcca gactcagttc gtatatatag acctattttc
     7741 aatttaacgt cgggcggcag cggcgggaat gggttttaga gctagaaata gcaagttaaa
     7801 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgcctac
     7861 ctggagcctg agagttgttc aataaaataa aaatgtttcg tttttttgct ttcgccagta
     7921 tttattattt ttcatcaata tgtattcaat ttggtatgta tttagtaatt gtaatatata
     7981 gacaatggtt ttccgttgac gtacatacat ctgacgtgtg tttatttaga cataatagtt
     8041 atgttttcac atctttttaa tgttcgctta atgcgtatgc atctcgagcc tatctatgca
     8101 aatctcagtg tgggatgtaa gccaagttga gtactttttc ttgaaatgat taaaatttct
     8161 tttgcaatat gtctatttgc gggacaatct ataacaaaaa gtttcgcttt gtttttttaa
     8221 aacatcctca taagcatctg aaacagctat tgaagctcta aatctgtcaa atattgcctg
     8281 ctgtttctca cgctttttct ctccagaact ccaagtctca aacaattctg aattggccaa
     8341 cctgaggttg tgacctaaaa atgtatattt tagtgttagt ttttattgtt aatttgtaca
     8401 cgtaaagcgg caattaaaag cactcaacac aaacaaatcc aacttgtgac gaccacaaac
     8461 atatagtacg aaccgctctc tttcgatgcc atgacaactt gtttgatata aacttagatt
     8521 ggtgctttat ttttaataat tttaaacgga ctcttttttc cgcagagtag ctttcgtgca
     8581 cccgtgatac cgaacagctg ttttaacagc ctatatattt taacagatcg tgactcagat
     8641 aaaatttctg ttacgtaaca ttgaagtttg attttaatat taacgacttt caaaaaatgg
     8701 atttttgccc acaaaacgtg atcagccagc ctaaagtgca ctcgctgcaa ataggaccgc
     8761 acgcgtaatg tattttagta ttttctacga gttaaattaa tttttgtatt tgacgtttcg
     8821 ggatgttttt aaaaaaggta gttgttgttg cggcagtacc cagtagcctt ccagcggctc
     8881 gaaactgaac atttgatctc atcctgaaaa gaaatattgc tatgctcatt tctaagaaaa
     8941 tgaccaacct ccatcttgtg tcctgcttgg caagactttg cacctgttgc accaaaagta
     9001 cattattttc actacgcttg cttgaataga attctcatcg gcgcgccttt ttgctcacct
     9061 gtgattgctc ctactcaaat acaaaaacat caaattttct gtcaataaag catatttatt
     9121 tatatttatt ttacaggaaa gaattccttt taaagtgtat tttaacctat aatgaaaaac
     9181 gattaaaaaa aatacataaa ataattcgaa aatttttgaa tagcccaggt tgataaaaat
     9241 tcatttcata cgttttataa cttatgcccc taagtatttt ttgaccatag tgtttcaatt
     9301 ctacattaat tttacagagt agaatgaaac gccacctact cagccaagag gcgaaaaggt
     9361 tagctcgcca agcagagagg gcgccagtgc tcactacttt ttataattct caacttcttt
     9421 ttccagactc agttcgtata tatagaccta ttttcaattt aacgtcggcc tcggggtcta
     9481 aagatgtgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     9541 aaaaagtggc accgagtcgg tgcttttttg cctacctgga gcctgagagt tgttcaataa
     9601 aataaaaatg tttcgttttt ttgctttcgc cagtatttat tatttttcat caatatgtat
     9661 tcaatttggt atgtatttag taattgtaat atatagacaa tggttttccg ttgacgtaca
     9721 tacatctgac gtgtgtttat ttagacataa tagttatgtt ttcacatctt tttaatgttc
     9781 gcttaatgcg tatgcatcct aggccggccg atctcggatc tgacaatgtt cagtgcagag
     9841 actcggctac gcctcgtgga ctttgaagtt gaccaacaat gtttattctt acctctaata
     9901 gtcctctgtg gcaaggtcaa gattctgtta gaagccaatg aagaacctgg ttgttcaata
     9961 acattttgtt cgtctaatat ttcactaccg cttgacgttg gctgcacttc atgtacctca
    10021 tctataaacg cttcttctgt atcgctctgg acgtcatctt cacttacgtg atctgatatt
    10081 tcactgtcag aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata
    10141 tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag
    10201 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
    10261 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
    10321 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
    10381 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
    10441 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
    10501 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
    10561 aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg
    10621 cgcgagcgtc gactctagag gatccccggg taccgagctc gaattcgtaa tcatggtcat
    10681 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10741 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10801 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10861 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10981 ggt
//
