LOCUS       Exported               11576 bp ds-DNA    circular SYN 04-¤T¤ë-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pNSSCP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Laboratory of Dr Hsiao-Chun Huang
  TITLE     Direct Submission
  JOURNAL   Exported 2022¦~2¤ë24¤é from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      2..59
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1025..1119
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1983..2026
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    2029..2050
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2051..2085
                     /note="J23116"
     RBS             2094..2108
                     /note="RBS_B0030"
     CDS             2115..2789
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /note="mRFP1"
                     /note="
                     "
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2814..3344
                     /codon_start=1
                     /note="popZ"
                     /translation="MSDQSQEPTMEEILASIRRIISEDDAPAEPAAEAAPPPPPEPEPE
                     PVSFDDEVLELTDPIAPEPELPPLETVGDIDVYSPPEPESEPAYTPPPAAPVFDRDEVA
                     EQLVGVSAASAAASAFGSLSSALLMPKDGRTLEDVVRELLRPLLKEWLDQNLPRIVETK
                     VEEEVQRISRGRGA"
     CDS             3345..3362
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /note="tetracysteine tag"
                     /translation="CCPGCC"
     terminator      3381..3426
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3384..3427
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3468..3495
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    3503
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      3531..3560
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(3623..4705)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    4840..4868
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4874..4893
                     /map="
                     "
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    4910..4924
                     /note="RBS_B0030"
     CDS             4939..5475
                     /label="29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, 
                     K98R, L106L, Q107K, T122S, A144T"
                     /note="29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A8"
     misc_feature    5476..5489
                     /label="linker"
                     /note="linker"
     misc_feature    5494..5504
                     /note="linker"
     CDS             5506..5955
                     /codon_start=1
                     /note="SpmX450"
                     /translation="MKPRHQVSRAAVDLIKRFEGYRQKAAQLPDGRWTVGYGHTLTARE
                     GASVSEKDAEALLLYDLISVAHSVNEHTYTPLNQNQFDALVCFAFNIGLDNFLRSGVLR
                     RINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKTLFLTP"
     terminator      5974..6019
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5977..6020
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6061..6088
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    6095..6096
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      6124..6153
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(6216..7298)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    7433..7461
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    7467..7486
                     /map="
                     "
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    7503..7517
                     /note="RBS_B0030"
     CDS             7524..7973
                     /codon_start=1
                     /note="SpmX450"
                     /translation="MKPRHQVSRAAVDLIKRFEGYRQKAAQLPDGRWTVGYGHTLTARE
                     GASVSEKDAEALLLYDLISVAHSVNEHTYTPLNQNQFDALVCFAFNIGLDNFLRSGVLR
                     RINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKTLFLTP"
     misc_feature    7974..7998
                     /note="linker"
     misc_feature    8007..10121
                     /label="T7 181+"
                     /note="T7 181+"
     misc_feature    8007..8034
                     /label="86 Split"
                     /note="86 Split"
     misc_feature    9269..9270
                     /label="split 600"
                     /note="split 600"
     terminator      10134..10188
                     /note="double terminator_B0015"
     terminator      10134..10139
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10155..10182
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    10189..10190
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    10191..10197
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    10198..10237
                     /note="T7 terminator"
     CDS             complement(10268..10981)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /note="superfolder GFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(11006..11494)
                     /codon_start=1
                     /note="DivIVA(B. subtilis)"
                     /translation="PLTPNDIHNKTFTKSFRGYDEDEVNEFLAQVRKDYEIVLRKKTEL
                     EAKVNELDERIGHFANIEETLNKSILVAQEAAEDVKRNSQKEAKLIVREAEKNADRIIN
                     ESLSKSRKIAMEIEELKKQSKVFRTRFQMLIEAQLDLLKNDDWDHLLEYEVDAVFEEKE
                     "
     misc_feature    11498..11526
                     /note="strong RBS"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     2041 gcttctagag ttgacagcta gctcagtcct agggactatg ctagctacta gagattaaag
     2101 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
     2161 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
     2221 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
     2281 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
     2341 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
     2401 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
     2461 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
     2521 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
     2581 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
     2641 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
     2701 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
     2761 gtgctgaagg tcgtcactcc accggtgctg gatcatctgg ctcatcggga tccatgtccg
     2821 atcagtctca agaacctaca atggaggaaa tcctcgcctc cattcgacgc atcatctcgg
     2881 aggatgacgc gccggcggag cctgcggccg aagcggcgcc cccgccgccg ccggaacccg
     2941 aacctgaacc ggtgtcgttc gacgacgagg ttctggaatt gacggacccg atcgcgcccg
     3001 agcccgagct gccgccgctg gagactgtcg gcgacatcga cgtctattcg ccgccggaac
     3061 ctgagtcgga accggcctac acgccgccgc cggcggctcc ggtgtttgat cgcgacgaag
     3121 tcgccgagca gctggtcggc gtttcggccg cttcggccgc ggcgagcgcc ttcggcagcc
     3181 tgagctcggc cctgctgatg cccaaggacg gtcggacgct ggaagacgtc gtacgcgagc
     3241 tgctgcgccc gctgctcaag gagtggctgg accagaacct gccgcgcatc gtcgagacca
     3301 aggttgagga agaagtgcag cgtatctctc ggggacgcgg cgcctgttgt ccaggatgtt
     3361 gttaaactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     3421 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     3481 gtgggccttt ctgcgtttat atactagaat attcagcaat ttgcccgtgc cgaagaaagg
     3541 cccacccgtg aaggtgagcc agtgagttga ttgctacgta atgtcggcca attcgcgcta
     3601 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     3721 tggtttttct tttcaccagt gagactggca acagctgatt gcccttcacc gcctggccct
     3781 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     3841 tggtggttaa cggcgggata taacatgagc tatcttcggt atcgtcgtat cccactaccg
     3901 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     3961 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     4021 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     4081 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     4141 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     4201 gtcgcgtacc gtcctcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     4261 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     4321 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     4381 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     4441 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     4501 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     4561 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     4621 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     4681 catcgtataa cgttactggt ttcatattca ccaccctgaa ttgactctct tccgggcgct
     4741 atcatgccat accgcgaaag gttttgcgcc attcgatggc gcgccgcttc gtcaggccac
     4801 atagctttct tgttctgatc ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc
     4861 gtataatgtg tggaattgtg agcgctcaca attctatgga ctatactaga ttaaagagga
     4921 gaaataccca agcttgggat gaacacgatt aacatcgcta agaacgactt ctctgacatc
     4981 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg ttcagctcgc
     5041 ggacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg
     5101 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc
     5161 actaccctac tccctaagat gattgcccgc atcaacgact ggtttgagga agtgaaagct
     5221 aagcgcggca ggcgcccgac agccttccag ttcctaaaag aaatcaagcc ggaagccgta
     5281 gcgtacatca ccattaagac ctctctggct tgcctaacca gtgctgacaa tacaaccgtt
     5341 caggctgtag caagcgcaat cggtcggacc attgaggacg aggctcgctt cggtcgtatc
     5401 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta
     5461 gggcacgtct acaagggtgg ctctggctcc gcggatccgg ttcaaatgaa accgcgtcat
     5521 caggtctccc gggccgccgt cgatctcatc aagagattcg aagggtaccg tcagaaggcc
     5581 gcgcagctcc ccgacgggcg ctggacggtc ggctatggcc acaccctgac cgcccgcgag
     5641 ggcgcctctg tctcggaaaa ggacgccgag gcgctgctgc tctatgatct catctcggtc
     5701 gcccattcgg tgaacgagca cacctacacg ccgctgaacc agaaccagtt cgacgcgttg
     5761 gtgtgcttcg ccttcaatat cggcctcgac aacttcctcc gctcgggcgt cctgcgccgg
     5821 atcaacgagg gctcgctgct gcaagccgcg tgcgcgatgg agatgtggcg caaggccgat
     5881 ttcgagggcg agcgcatcgt catcgatgca ctggttcgcc gccgctccgc cgagaagact
     5941 ctgttcctca cgccgtaaac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     6001 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     6061 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aatattcagc aatttgcccg
     6121 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaatgtcgg
     6181 ccaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6301 ttgggcgcca gggtggtttt tcttttcacc agtgagactg gcaacagctg attgcccttc
     6361 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6421 aaatcctgtt tgatggtggt taacggcggg atataacatg agctatcttc ggtatcgtcg
     6481 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     6541 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     6601 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     6661 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     6721 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     6781 tgctccacgc ccagtcgcgt accgtcctca tgggagaaaa taatactgtt gatgggtgtc
     6841 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     6901 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     6961 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     7021 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     7081 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     7141 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     7201 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     7261 gcatactctg cgacatcgta taacgttact ggtttcatat tcaccaccct gaattgactc
     7321 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggcgcgccgc
     7381 ttcgtcaggc cacatagctt tcttgttctg atcggaacga tcgttggctg tgttgacaat
     7441 taatcatcgg ctcgtataat gtgtggaatt gtgagcgctc acaattctat ggactatact
     7501 agattaaaga ggagaaatac tagatgaaac cgcgtcatca ggtctcccgg gccgccgtcg
     7561 atctcatcaa gagattcgaa gggtaccgtc agaaggccgc gcagctcccc gacgggcgct
     7621 ggacggtcgg ctatggccac accctgaccg cccgcgaggg cgcctctgtc tcggaaaagg
     7681 acgccgaggc gctgctgctc tatgatctca tctcggtcgc ccattcggtg aacgagcaca
     7741 cctacacgcc gctgaaccag aaccagttcg acgcgttggt gtgcttcgcc ttcaatatcg
     7801 gcctcgacaa cttcctccgc tcgggcgtcc tgcgccggat caacgagggc tcgctgctgc
     7861 aagccgcgtg cgcgatggag atgtggcgca aggccgattt cgagggcgag cgcatcgtca
     7921 tcgatgcact ggttcgccgc cgctccgccg agaagactct gttcctcacg ccgggagcct
     7981 ccaccagaac cgcctccgga tccgcgaaag catttatgca agttgtcgag gctgacatgc
     8041 tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc
     8101 atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc
     8161 gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg
     8221 ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt
     8281 gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc
     8341 gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg
     8401 tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca
     8461 acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg
     8521 acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc
     8581 ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc
     8641 gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc
     8701 ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa
     8761 tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac
     8821 caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg
     8881 ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg
     8941 cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct
     9001 tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct
     9061 cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc
     9121 gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct
     9181 acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg
     9241 ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc
     9301 tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta
     9361 agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc
     9421 tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga
     9481 atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag
     9541 ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca
     9601 aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg
     9661 atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt
     9721 tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg
     9781 cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc
     9841 ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc
     9901 acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa
     9961 ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg
    10021 accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga
    10081 acctccgtga catcttagag tcggacttcg cgttcgcgta acccaagctt ggggctctct
    10141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaaa
    10201 aaacccctca agacccgttt agaggcccca aggggttatg ctaggatcct tattaagcac
    10261 cttattattt gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa
    10321 gaaggaccat gtggtcacgc ttttcgttgg gatctttcga aaggacagat tgtgtcgaca
    10381 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
    10441 ggtctgctag ttgaacggaa ccatcttcaa cgttgtggcg aattttgaag ttagctttga
    10501 ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttaaag ttgtactcga
    10561 gtttgtgtcc aagaatgttt ccatcttctt taaaatcaat accctttaac tcgatacgat
    10621 taacaagggt atcaccttca aacttgactt cagcacgcgt cttgtaggtc ccgtcatctt
    10681 tgaaagatat agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
    10741 gccgtttcat gtgatccgga taacgggaaa agcattgaac accataggtc agagtagtga
    10801 caagtgttgg ccacggaaca ggtagttttc cagtagtgca aataaattta agggtgagtt
    10861 ttccgtttgt agcatcacct tcaccctctc cacggacaga aaatttgtgc ccattaacat
    10921 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctca
    10981 tcagcagaaa ttcaatatcc agtttttcct tttcctcaaa tactgcgtcg acttcatact
    11041 caaggagatg atcccaatcg tcatttttca gaagatcaag ctgagcttca atcagcattt
    11101 ggaaacgtgt tctgaacact ttagactgtt ttttcagctc ttcaatttcc attgcaattt
    11161 ttcttgattt tgataacgat tcgttgataa tgcgatcagc gtttttctcc gcttcccgaa
    11221 cgatcagctt tgcttctttt tgagaattgc gttttacgtc ttcagccgct tcttgagcaa
    11281 ctaaaattga tttattcaat gtctcctcaa tattggcaaa gtgtccgatt ctttcatcaa
    11341 gctcattgac tttcgcttca agctcagttt tcttgcggag aacaatttcg taatcttttc
    11401 tgacttgggc taggaactca tttacttcat cttcatcata tccgcgaaaa ctttttgtaa
    11461 acgtcttgtt gtgaatatca tttggcgtta atggcatctt tacctcctct atcgcggaaa
    11521 ttgacaggat cctctcccta tagtgagtcg tattacgact agtagcggcc gctgca
//
