LOCUS       Exported                9818 bp ds-DNA    circular SYN 01-¤Q¤G¤ë-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    116RP-pTac-TEV(N)-SpmX-pTac-SpmX-TEV(C)-116DivIVA-GFP-tevR-M0050...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Joe
  TITLE     Direct Submission
  JOURNAL   Exported 2022¦~2¤ë25¤é from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      28..57
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(120..1202)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    1337..1365
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1371..1390
                     /map="
                     "
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    1403..1406
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1407..1421
                     /note="RBS_B0030"
     CDS             1434..1787
                     /codon_start=1
                     /label="TEV-S219V"
                     /note="TEV-S219V N354"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     CDS             1434..1787
                     /codon_start=1
                     /product="SpmX450::TEV-S219V-N354"
                     /note="SpmX450::TEVN"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     misc_feature    1806..1817
                     /note="Linker"
     CDS             1818..2267
                     /codon_start=1
                     /note="SpmX450"
                     /translation="MKPRHQVSRAAVDLIKRFEGYRQKAAQLPDGRWTVGYGHTLTARE
                     GASVSEKDAEALLLYDLISVAHSVNEHTYTPLNQNQFDALVCFAFNIGLDNFLRSGVLR
                     RINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKTLFLTP"
     misc_feature    2272..2277
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2286..2331
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2289..2332
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2373..2400
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    2407..2408
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      2436..2465
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(2528..3610)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    3745..3773
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3779..3798
                     /map="
                     "
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    3818..3829
                     /note="RBS_B0034"
     CDS             3836..4285
                     /codon_start=1
                     /note="SpmX450"
                     /translation="MKPRHQVSRAAVDLIKRFEGYRQKAAQLPDGRWTVGYGHTLTARE
                     GASVSEKDAEALLLYDLISVAHSVNEHTYTPLNQNQFDALVCFAFNIGLDNFLRSGVLR
                     RINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKTLFLTP"
     misc_feature    4286..4330
                     /note="Linker"
     CDS             4311..4705
                     /codon_start=1
                     /note="SpmX450::TEVC"
                     /translation="SGSGSGKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVS
                     TRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKV
                     FMVKPEEPFQPVKEATQLMNELVYSQ"
     CDS             4331..4702
                     /codon_start=1
                     /label="TEV-S219V"
                     /note="TEV-S219V 372C"
                     /translation="KSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMNELVYSQ"
     terminator      4717..4771
                     /note="double terminator_B0015"
     terminator      4717..4722
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4738..4765
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    4772..4773
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    4774..4779
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        4780..4814
                     /note="J23116"
     RBS             4843..4854
                     /note="B0034"
     CDS             4866..5354
                     /codon_start=1
                     /note="DivIVA"
                     /translation="PLTPNDIHNKTFTKSFRGYDEDEVNEFLAQVRKDYEIVLRKKTEL
                     EAKVNELDERIGHFANIEETLNKSILVAQEAAEDVKRNSQKEAKLIVREAEKNADRIIN
                     ESLSKSRKIAMEIEELKKQSKVFRTRFQMLIEAQLDLLKNDDWDHLLEYEVDAVFEEKE
                     "
     CDS             5385..6098
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /note="superfolder GFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6099..6119
                     /codon_start=1
                     /note="TEVRs(ENLYFQS)"
                     /translation="ENLYFQS"
     misc_feature    6120..6152
                     /note="M0050 Degradation tag"
     terminator      6167..6295
                     /note="B0015"
     misc_feature    6296..6297
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    6316
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     rep_origin      complement(6569..7157)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      7194..7221
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    7228..7229
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    7241..7242
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    7248
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    7261
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      7340..7434
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(7455..8114)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      8139..8166
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    8173..8174
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    8193
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    8344..8365
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        8366..8400
                     /note="J23116"
     RBS             8409..8423
                     /note="RBS_B0030"
     CDS             8430..9104
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /note="mRFP1"
                     /note="
                     "
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             9129..9659
                     /codon_start=1
                     /note="popZ"
                     /translation="MSDQSQEPTMEEILASIRRIISEDDAPAEPAAEAAPPPPPEPEPE
                     PVSFDDEVLELTDPIAPEPELPPLETVGDIDVYSPPEPESEPAYTPPPAAPVFDRDEVA
                     EQLVGVSAASAAASAFGSLSSALLMPKDGRTLEDVVRELLRPLLKEWLDQNLPRIVETK
                     VEEEVQRISRGRGA"
     CDS             9564..9566
                     /codon_start=1
                     /note="Feature 16"
                     /translation="P"
     CDS             9585..9587
                     /codon_start=1
                     /note="Feature 14"
                     /translation="D"
     CDS             9618..9620
                     /codon_start=1
                     /note="Feature 15"
                     /translation="V"
     CDS             9660..9677
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /note="tetracysteine tag"
                     /translation="CCPGCC"
     terminator      9696..9741
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9699..9742
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9783..9810
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    9818
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
ORIGIN
        1 ctagaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
       61 gagttgattg ctacgtaatg tcggccaatt cgcgctaact tacattaatt gcgttgcgct
      121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      181 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
      241 actggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
      301 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
      361 catgagctat cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
      421 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
      481 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
      541 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
      601 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
      661 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ctcatgggag
      721 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
      781 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
      841 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
      901 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
      961 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     1021 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     1081 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     1141 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     1201 atattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     1261 ttgcgccatt cgatggcgcg ccgcttcgtc aggccacata gctttcttgt tctgatcgga
     1321 acgatcgttg gctgtgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc
     1381 gctcacaatt ctatggacta tactagatta aagaggagaa atacccaagc ttgggagaga
     1441 gcttgtttaa gggaccacgt gattacaacc cgatatcgag caccatttgt catttaacga
     1501 atgaatctga tgggcacaca acatcgttgt atggtattgg atttggtccc ttcatcatta
     1561 caaacaagca cttgtttaga agaaataatg gaacactgtt ggtccaatca ctacatggtg
     1621 tattcaaggt caagaacacc acgactttgc aacaacacct cattgatggg agggacatga
     1681 taattattcg catgcctaag gatttcccac catttcctca aaagctgaaa tttagagagc
     1741 cacaaaggga agagcgcata tgtcttgtga caaccaactt ccaaactcaa acagagcaga
     1801 acgcggatcc ggttcaaatg aaaccgcgtc atcaggtctc ccgggccgcc gtcgatctca
     1861 tcaagagatt cgaagggtac cgtcagaagg ccgcgcagct ccccgacggg cgctggacgg
     1921 tcggctatgg ccacaccctg accgcccgcg agggcgcctc tgtctcggaa aaggacgccg
     1981 aggcgctgct gctctatgat ctcatctcgg tcgcccattc ggtgaacgag cacacctaca
     2041 cgccgctgaa ccagaaccag ttcgacgcgt tggtgtgctt cgccttcaat atcggcctcg
     2101 acaacttcct ccgctcgggc gtcctgcgcc ggatcaacga gggctcgctg ctgcaagccg
     2161 cgtgcgcgat ggagatgtgg cgcaaggccg atttcgaggg cgagcgcatc gtcatcgatg
     2221 cactggttcg ccgccgctcc gccgagaaga ctctgttcct cacgccgtaa actagagcca
     2281 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2341 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2401 tttatatact agaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt
     2461 gagccagtga gttgattgct acgtaatgtc ggccaattcg cgctaactta cattaattgc
     2521 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     2641 ccagtgagac tggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     2701 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     2761 ggatataaca tgagctatct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     2821 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     2881 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     2941 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     3001 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     3061 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct
     3121 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     3181 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     3241 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     3301 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     3361 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     3421 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     3481 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     3541 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     3601 ctggtttcat attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     3661 gaaaggtttt gcgccattcg atggcgcgcc gcttcgtcag gccacatagc tttcttgttc
     3721 tgatcggaac gatcgttggc tgtgttgaca attaatcatc ggctcgtata atgtgtggaa
     3781 ttgtgagcgc tcacaattct atggactata ctagattaaa gaggagaaat actagatgaa
     3841 accgcgtcat caggtctccc gggccgccgt cgatctcatc aagagattcg aagggtaccg
     3901 tcagaaggcc gcgcagctcc ccgacgggcg ctggacggtc ggctatggcc acaccctgac
     3961 cgcccgcgag ggcgcctctg tctcggaaaa ggacgccgag gcgctgctgc tctatgatct
     4021 catctcggtc gcccattcgg tgaacgagca cacctacacg ccgctgaacc agaaccagtt
     4081 cgacgcgttg gtgtgcttcg ccttcaatat cggcctcgac aacttcctcc gctcgggcgt
     4141 cctgcgccgg atcaacgagg gctcgctgct gcaagccgcg tgcgcgatgg agatgtggcg
     4201 caaggccgat ttcgagggcg agcgcatcgt catcgatgca ctggttcgcc gccgctccgc
     4261 cgagaagact ctgttcctca cgccgggagc ctccaccaga accgcctccg gatccggtag
     4321 cggttcgggt aagagcatgt ctagcatggt gtcagacacc agttgcacat tcccttcatc
     4381 tgatggcata ttctggaagc attggattca aaccaaggat gggcagtgtg gcagtccatt
     4441 agtatcaact agagatgggt tcattgttgg tatacactca gcatcgaatt tcaccaacac
     4501 aaacaattat ttcacaagcg tgccgaaaaa cttcatggaa ttgttgacaa atcaggaggc
     4561 gcagcagtgg gttagtggtt ggcgattaaa tgctgactca gtattgtggg ggggccataa
     4621 agttttcatg gtgaaacctg aagagccttt tcagccagtt aaggaagcga ctcaactcat
     4681 gaatgaattg gtgtactcgc aataaccaag cttggggctc tctactagag tcacactggc
     4741 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacagctag ctcagtccta
     4801 gggactatgc tagctactag agtttacggc tagctactag agaaagagga gaaaaagctt
     4861 ctatgccatt aacgccaaat gatattcaca acaagacgtt tacaaaaagt tttcgcggat
     4921 atgatgaaga tgaagtaaat gaatttctag cccaagtcag aaaagattac gaaattgttc
     4981 tccgcaagaa aactgagctt gaagcgaaag tcaatgagct tgatgaaaga atcggacact
     5041 ttgccaatat tgaggagaca ttgaataaat caattttagt tgctcaagaa gcggctgaag
     5101 acgtaaaacg caattctcaa aaagaagcaa agctgatcgt tcgggaagcg gagaaaaacg
     5161 ctgatcgcat tatcaacgaa tcgttatcaa aatcaagaaa aattgcaatg gaaattgaag
     5221 agctgaaaaa acagtctaaa gtgttcagaa cacgtttcca aatgctgatt gaagctcagc
     5281 ttgatcttct gaaaaatgac gattgggatc atctccttga gtatgaagtc gacgcagtat
     5341 ttgaggaaaa ggaaaaactg gatattgaat ttctgcaagt accgatgagc aaaggagaag
     5401 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     5461 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
     5521 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
     5581 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
     5641 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
     5701 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
     5761 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta
     5821 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     5881 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
     5941 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
     6001 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     6061 ctgctgggat tacacatggc atggatgagc tctacaaaga aaacctgtat tttcagagcg
     6121 ctgctaacga cgaaaactac gctctggctg cttaataata ctagagccag gcatcaaata
     6181 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6241 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     6301 gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
     6361 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
     6421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6541 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
     6601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7321 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     7381 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     7441 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     7501 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     7561 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     7621 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     7681 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     7741 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     7801 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     7861 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     7921 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     7981 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     8041 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     8101 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     8161 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac
     8221 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8281 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     8341 ctggaattcg cggccgcttc tagagttgac agctagctca gtcctaggga ctatgctagc
     8401 tactagagat taaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     8461 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     8521 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     8581 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     8641 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     8701 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     8761 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     8821 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     8881 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     8941 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     9001 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     9061 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgctggatca tctggctcat
     9121 cgggatccat gtccgatcag tctcaagaac ctacaatgga ggaaatcctc gcctccattc
     9181 gacgcatcat ctcggaggat gacgcgccgg cggagcctgc ggccgaagcg gcgcccccgc
     9241 cgccgccgga acccgaacct gaaccggtgt cgttcgacga cgaggttctg gaattgacgg
     9301 acccgatcgc gcccgagccc gagctgccgc cgctggagac tgtcggcgac atcgacgtct
     9361 attcgccgcc ggaacctgag tcggaaccgg cctacacgcc gccgccggcg gctccggtgt
     9421 ttgatcgcga cgaagtcgcc gagcagctgg tcggcgtttc ggccgcttcg gccgcggcga
     9481 gcgccttcgg cagcctgagc tcggccctgc tgatgcccaa ggacggtcgg acgctggaag
     9541 acgtcgtacg cgagctgctg cgcccgctgc tcaaggagtg gctggaccag aacctgccgc
     9601 gcatcgtcga gaccaaggtt gaggaagaag tgcagcgtat ctctcgggga cgcggcgcct
     9661 gttgtccagg atgttgttaa actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     9721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     9781 ctggctcacc ttcgggtggg cctttctgcg tttatata
//
