LOCUS       pREC050                 5020 bp ds-DNA     circular     22-FEB-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     misc_feature    1..313
                     /label="PiggyBac R-border"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          132..153
                     /label="HK502"
                     /note="sequence: gcgtgcttgtcaatgcggtaag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          177..198
                     /label="HK542"
                     /note="sequence: gaccgcgtgagtcaaaatgacg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          313..344
                     /label="HK534"
                     /note="sequence: caactagaatgctagcgctgatatctccaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    358..376
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    358..376
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    394..412
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    394..412
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    430..448
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    430..448
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    466..484
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    466..484
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    502..520
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    502..520
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    538..556
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    538..556
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    574..592
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    574..592
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    604..698
                     /label="minCMV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          611..630
                     /label="HK160"
                     /note="sequence: tgtacggtgggcgcctataa"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          621..642
                     /label="HK094"
                     /note="sequence: gcgcctataaaagcagagctcg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             743..1441
                     /label="TagBFP"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     primer          complement(792..814)
                     /label="HK360"
                     /note="sequence: cttgaagtgatggttgtccacgg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1017..1036
                     /label="oTG_so4"
                     /note="sequence: TCACCACATACGAAGACGGG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1158..1190)
                     /label="HK365"
                     /note="sequence: CAGCaGGaTACAGaGTCTCGGTGAAGGCCTCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1173..1201
                     /label="HK366"
                     /note="sequence: AGACtCTGTAtCCtGCTGACGGCGGCCTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    1448..1557
                     /label="Triplex"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     primer          1609..1656
                     /label="HK835"
                     /note="sequence: GATCCTTTCTTTCTTTGaatttctactaagtgtagatactcgatTCAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1613..1662)
                     /label="HK836"
                     /note="sequence: tgttaaCTGAatcgagtatctacacttagtagaaattCAAAGAAAGAAAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1626..1645
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1646..1668
                     /label="crGFP* ABE3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1657..1707
                     /label="HK837"
                     /note="sequence: ttaacaagggtaAATTTCTACTAAGTGTAGATCGAGTTTTCTTTTTGTTTC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(1663..1711)
                     /label="HK838"
                     /note="sequence: TCGAGAAACAAAAAGAAAACTCGATCTACACTTAGTAGAAATTtaccct"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1669..1688
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1713..1844
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(1798..1824)
                     /label="HK615"
                     /note="sequence: cattgatgagtttggacaaaccacaac"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1844..1883)
                     /label="HK831"
                     /note="sequence: ttttaactagactagtcggttagcttgagttatccagttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1845..1867
                     /label="amplified w/ sv40"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1886..2120
                     /label="PiggyBac L-border"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2161..2180)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2198..2218)
                     /label="M13-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(2224..2246)
                     /label="LacO site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2297..2316)
                     /label="CRP binding site (lacZYA MG1655)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(2586..3268)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(3366..4025)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4265..4293)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(4486..4792)
                     /label="F1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(4811..4879)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     4950..4967
                     /label="M13-fwd primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     4976..4996
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4977..4995
                     /label="T7 promoter (Ambion)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcaactaga atgctagcgc tgatatctcc accccacagt ggtttactcc
      361 ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc
      421 agactttact ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata
      481 gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc
      541 ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga gaacgtataa
      601 gctttaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga accgtcagat
      661 cgcctggagc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
      721 ctcgtaaaga attcccacca tgAGCGAGCT GATTAAGGAG AACATGCACA TGAAGCTGTA
      781 CATGGAGGGC ACCGTGGACA ACCATCACTT CAAGTGCACA TCCGAGGGCG AAGGCAAGCC
      841 CTACGAGGGC ACCCAGACCA TGAGAATCAA GGTGGTCGAG GGCGGCCCTC TCCCCTTCGC
      901 CTTCGACATC CTGGCTACTA GCTTCCTCTA CGGCAGCAAG ACCTTCATCA ACCACACCCA
      961 GGGCATCCCC GACTTCTTCA AGCAGTCCTT CCCTGAGGGC TTCACATGGG AGAGAGTCAC
     1021 CACATACGAA GACGGGGGCG TGCTGACCGC TACCCAGGAC ACCAGCCTCC AGGACGGCTG
     1081 CCTCATCTAC AACGTCAAGA TCAGAGGGGT GAACTTCACA TCCAACGGCC CTGTGATGCA
     1141 GAAGAAAACA CTCGGCTGGG AGGCCTTCAC CGAGACtCTG TAtCCtGCTG ACGGCGGCCT
     1201 GGAAGGCAGA AACGACATGG CCCTGAAGCT CGTGGGCGGG AGCCATCTGA TCGCAAACAT
     1261 CAAGACCACA TATAGATCCA AGAAACCCGC TAAGAACCTC AAGATGCCTG GCGTCTACTA
     1321 TGTGGACTAC AGACTGGAAA GAATCAAGGA GGCCAACAAC GAGACCTACG TCGAGCAGCA
     1381 CGAGGTGGCA GTGGCCAGAT ACTGCGACCT CCCTAGCAAA CTGGGGCACA AGCTTAATta
     1441 aACGCGTGAT TCGTCAGTAG GGTTGTAAAG GTTTTTCTTT TCCTGAGAAA ACAACCTTTT
     1501 GTTTTCTCAG GTTTTGCTTT TTGGCCTTTC CCTAGCTTTA AAAAAAAAAA AGCAAAActc
     1561 accgaggcag ttccatagga tggcaagatc ctggtattgg tctgcgaGGA TCCTTTCTTT
     1621 CTTTGaattt ctactaagtg tagatactcg atTCAGttaa caagggtaAA TTTCTACTAA
     1681 GTGTAGATCG AGTTTTCTTT TTGTTTCTCG AGttgtttat tgcagcttat aatggttaca
     1741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     1861 ctaaccgact agtctagtta aaagttttgt tactttatag aagaaatttt gagtttttgt
     1921 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     1981 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     2041 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     2101 gattatcttt ctagggttaa tctagtgttt aaacggccgc ttaattaatc cagcttttgt
     2161 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4321 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     4381 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4441 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4561 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4621 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4801 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     4981 acgactcact atagggcgaa ttggggcgcg ccattctaga
//