LOCUS       Exported                7509 bp DNA     circular SYN 26-JAN-2022
DEFINITION  Membrane bound expression of human CD19 antigen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7509
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(2..596,3990..5819,597..3184)
                     /mol_type="other DNA"
                     /note="This feature has 3 segments:
                        1:    2 ..  596 / #ffffff
                        2: 3990 .. 5819 / #ffffff
                        3:  597 .. 3184 / #ffffff"
                     /organism="synthetic DNA construct"
     source          join(7507..7509,5817..5819)
                     /mol_type="other DNA"
                     /note="This feature has 2 segments:
                        1: 7507 .. 7509 / #ffffff
                        2: 5817 .. 5819 / #ffffff"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(49..273)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             810..1670
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     polyA_site      complement(2992..3047)
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(3187..3981)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII) CDS
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(3978..3987)
                     /note="/created by=Sarah"
                     /note="/modified by=Sarah"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3996..4607)
                     /label=RPBSA
                     /note="/created by=Sarah"
                     /note="/modified by=Sarah"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4615..5796
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation  
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     misc_difference 5822..5827
                     /label=Kozak
                     /note="/created by=Sarah"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             5828..7501
                     /label=Hu CD19
                     /note="/created by=Sarah"
                     /note="color: #993366; direction: RIGHT"
ORIGIN
        1 aggccaagct tggggatcaa ttctctagag ctcgctgatc agcctcgact gtgccttcta
       61 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
      301 cctactagat agcggacccc ttaccgaaac atcgccgcat tctgcagagg agtcgaactt
      361 agtgtatgta aacttctgac ccactggaat tgtgatacag tgaattataa gtgaaataat
      421 ctgtctgtaa acaattgttg gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac
      481 tgacttgcca aaactattgt ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt
      541 ttaatgactc caacttaagt gtatgtaaac ttccgacttc aactgtatag ggatccaatt
      601 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
      661 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggatc cctatacagt tgaagtcgga agtttacata
     2641 caccttagcc aaatacattt aaactcactt tttcacaatt cctgacattt aatcctagta
     2701 aaaattccct gtcttaggtc agttaggatc accactttat tttaagaatg tgaaatatca
     2761 gaataatagt agagagaatg attcatttca gcttttattt ctttcatcac attcccagtg
     2821 ggtcagaagt ttacatacac tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg
     2881 tcccgagtag ctagttcatg gcagccagca tatggcatat gttgccaaac tctaaaccaa
     2941 atactcattc tgatgtttta aatgatttgc cctcccatat gtccttccga gtgagagaca
     3001 caaaaaattc caacacacta ttgcaatgaa aataaatttc ctttattagc cagaagtcag
     3061 atgctcaagg ggcttcatga tgtccccata atttttggca gagggaaaaa gatctcagtg
     3121 gtatttgtga gccagggcat tggccacacc agccaccacc ttctgatagg cagcctgcac
     3181 ctgaggtcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     3241 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     3301 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     3361 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     3421 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     3481 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     3541 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     3601 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     3661 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     3721 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     3781 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     3841 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     3901 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     3961 caatccatct tgttcaatca tggtggccct caggtgcaga ggtttctaca gggaaagaga
     4021 gaaaggctac agttagtttg ctgtgatgat gctaagtgcc gaggtcagat gtctatggat
     4081 tgtacacgga atcccaacgc aggaaccaaa aaacactcac ccaaaaaaat ggcgctaagg
     4141 ccgagagaaa ggcgaagttc cgtctacggc tatatacctt gaaacagggg cggcagaagt
     4201 gacgcaacgc ggttgcgcgg acggcttcct gtgttttcat tggtcgaagc gttctgcgcg
     4261 tgcgcagaga ttccccaggg aaggcgggcc ctcttccgaa tgtccaatcc ggacattctt
     4321 ccggttggac ctccgcggtc agcgctgtgc ccggcgagag atcacgtggg gcgcggaggc
     4381 ggtgctgctg gggcacggcc gtccagcctc ggcggccata tttttgaggg gctgttcatc
     4441 tcgttcacac gctctgtccg ccatgtttgt gagtggaagc gccattaccc cttcaagcga
     4501 ctgaaggctg cagggcctct ggtggcccgc atggggagac cagacccgcc aggcccgcct
     4561 ttccgcactc agtccgggct tactttattt tgtgagacag ggtctcgcct agaggctccg
     4621 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     4681 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     4741 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     4801 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     4861 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     4921 cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     4981 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcttggcct
     5041 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     5101 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     5161 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     5221 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     5281 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     5341 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     5401 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     5461 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     5521 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     5581 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     5641 tttccccata ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     5701 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     5761 gttcaaagtt tttttcttcc atttaaggtg tcgtgaaaac taccccaagc tggcctctga
     5821 ggccaccatg ccacctcctc gcctcctctt cttcctcctc ttcctcaccc ccatggaagt
     5881 caggcccgag gaacctctag tggtgaaggt ggaagaggga gataacgctg tgctgcagtg
     5941 cctcaagggg acctcagatg gccccactca gcagctgacc tggtctcggg agtccccgct
     6001 taaacccttc ttaaaactca gcctggggct gccaggcctg ggaatccaca tgaggcccct
     6061 ggccatctgg cttttcatct tcaacgtctc tcaacagatg gggggcttct acctgtgcca
     6121 gccggggccc ccctctgaga aggcctggca gcctggctgg acagtcaatg tggagggcag
     6181 cggggagctg ttccggtgga atgtttcgga cctaggtggc ctgggctgtg gcctgaagaa
     6241 caggtcctca gagggcccca gctccccttc cgggaagctc atgagcccca agctgtatgt
     6301 gtgggccaaa gaccgccctg agatctggga gggagagcct ccgtgtctcc caccgaggga
     6361 cagcctgaac cagagcctca gccaggacct caccatggcc cctggctcca cactctggct
     6421 gtcctgtggg gtaccccctg actctgtgtc caggggcccc ctctcctgga cccatgtgca
     6481 ccccaagggg cctaagtcat tgctgagcct agagctgaag gacgatcgcc cggccagaga
     6541 tatgtgggta atggagacgg gtctgttgtt gccccgggcc acagctcaag acgctggaaa
     6601 gtattattgt caccgtggca acctgaccat gtcattccac ctggagatca ctgctcggcc
     6661 agtactatgg cactggctgc tgaggactgg tggctggaag gtctcagctg tgactttggc
     6721 ttatctgatc ttctgcctgt gttcccttgt gggcattctt catcttcaaa gagccctggt
     6781 cctgaggagg aaaagaaagc gaatgactga ccccaccagg agattcttca aagtgacgcc
     6841 tcccccagga agcgggcccc agaaccagta cgggaacgtg ctgtctctcc ccacacccac
     6901 ctcaggcctc ggacgcgccc agcgttgggc cgcaggcctg gggggcactg ccccgtctta
     6961 tggaaacccg agcagcgacg tccaggcgga tggagccttg gggtcccgga gcccgccggg
     7021 agtgggccca gaagaagagg aaggggaggg ctatgaggaa cctgacagtg aggaggactc
     7081 cgagttctat gagaacgact ccaaccttgg gcaggaccag ctctcccagg atggcagcgg
     7141 ctacgagaac cctgaggatg agcccctggg tcctgaggat gaagactcct tctccaacgc
     7201 tgagtcttat gagaacgagg atgaagagct gacccagccg gtcgccagga caatggactt
     7261 cctgagccct catgggtcag cctgggaccc cagccgggaa gcaacctccc tggcagggtc
     7321 ccagtcctat gaggatatga gaggaatcct gtatgcagcc ccccagctcc gctccattcg
     7381 gggccagcct ggacccaatc atgaggaaga tgcagactct tatgagaaca tggataatcc
     7441 cgatgggcca gacccagcct ggggaggagg gggccgcatg ggcacctgga gcaccaggtg
     7501 aggcctgtc
//
