LOCUS       Exported                8052 bp DNA     circular SYN 26-JAN-2022
DEFINITION  Soluble expression of human extracellular Her2-Fc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8052
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(7099..8052,2667..7098)
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="This feature has 2 segments:
                        1: 7099 .. 8052 / #ffffff
                        2: 2667 .. 7098 / #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    17..21
                     /label=KOZAK
                     /note="/created_by=User"
                     /note="/modified_by=mclal85p"
                     /note="color: #ffdd55; direction: RIGHT"
     sig_peptide     22..87
                     /note="/created by=Morad-Remy"
                     /note="/modified by=Morad-Remy"
                     /note="color: #cc99b2; direction: RIGHT"
     mat_peptide     88..1982
                     /label=Her-2 EC domain
                     /note="/created by=Morad-Remy"
                     /note="color: #cc99b2; direction: RIGHT"
     misc_feature    1984..2013
                     /label=Hinge
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    2014..2343
                     /label=CH2
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ca6502"
     misc_feature    2344..2664
                     /label=CH3
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     polyA_signal    2698..2922
                     /label=bGH poly(A) signal polyA signal
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      2968..3396
                     /direction=RIGHT
                     /label=f1 ori rep origin
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     promoter        3410..3739
                     /label=SV40 promoter
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3590..3725
                     /direction=RIGHT
                     /label=SV40 ori rep origin
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             3806..4600
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII) CDS
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4774..4895
                     /label=SV40 poly(A) signal polyA signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    4968..4984
                     /label=lac operator protein bind
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(4992..5022)
                     /label=lac promoter
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments: 1: 
                     3321 .. 3327 / #ffffff / -10 2: 3328 .. 3345 / #ffffff 3: 
                     3346 .. 3351 / #ffffff / -35"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(5346..5931)
                     /direction=LEFT
                     /label=ori rep origin
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             complement(6102..6962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla CDS
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments: 1: 4431 .. 5222 / 
                     #ccffcc 2: 5223 .. 5291 / #ccffcc / signal sequence 
                     Cleavage site after base 5222"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6963..7067)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        7333..7712
                     /label=CMV enhancer
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        7713..7916
                     /label=CMV promoter
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7961..7979
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 aattcggcct ctgaggccac catggagctg gcggccttgt gccgctgggg gctcctcctc
       61 gccctcttgc cccccggagc cgcgagcacc caagtgtgca ccggcacaga catgaagctg
      121 cggctccctg ccagtcccga gacccacctg gacatgctcc gccacctcta ccagggctgc
      181 caggtggtgc agggaaacct ggaactcacc tacctgccca ccaatgccag cctgtccttc
      241 ctgcaggata tccaggaggt gcagggctac gtgctcatcg ctcacaacca agtgaggcag
      301 gtcccactgc agaggctgcg gattgtgcga ggcacccagc tctttgagga caactatgcc
      361 ctggccgtgc tagacaatgg agacccgctg aacaatacca cccctgtcac aggggcctcc
      421 ccaggaggcc tgcgggagct gcagcttcga agcctcacag agatcttgaa aggaggggtc
      481 ttgatccagc ggaaccccca gctctgctac caggacacga ttttgtggaa ggacatcttc
      541 cacaagaaca accagctggc tctcacactg atagacacca accgctctcg ggcctgccac
      601 ccctgttctc cgatgtgtaa gggctcccgc tgctggggag agagttctga ggattgtcag
      661 agcctgacgc gcactgtctg tgccggtggc tgtgcccgct gcaaggggcc actgcccact
      721 gactgctgcc atgagcagtg tgctgccggc tgcacgggcc ccaagcactc tgactgcctg
      781 gcctgcctcc acttcaacca cagtggcatc tgtgagctgc actgcccagc cctggtcacc
      841 tacaacacag acacgtttga gtccatgccc aatcccgagg gccggtatac attcggcgcc
      901 agctgtgtga ctgcctgtcc ctacaactac ctttctacgg acgtgggatc ctgcaccctc
      961 gtctgccccc tgcacaacca agaggtgaca gcagaggatg gaacacagcg gtgtgagaag
     1021 tgcagcaagc cctgtgcccg agtgtgctat ggtctgggca tggagcactt gcgagaggtg
     1081 agggcagtta ccagtgccaa tatccaggag tttgctggct gcaagaagat ctttgggagc
     1141 ctggcatttc tgccggagag ctttgatggg gacccagcct ccaacactgc cccgctccag
     1201 ccagagcagc tccaagtgtt tgagactctg gaagagatca caggttacct atacatctca
     1261 gcatggccgg acagcctgcc tgacctcagc gtcttccaga acctgcaagt aatccgggga
     1321 cgaatcctgc acaatggcgc ctactcgctg accctgcaag ggctgggcat cagctggctg
     1381 gggctgcgct cactgaggga actgggcagt ggactggccc tcatccacca taacacccac
     1441 ctctgcttcg tgcacacggt gccctgggac cagctctttc ggaacccgca ccaagctctg
     1501 ctccacactg ccaaccggcc agaggacgag tgtgtgggcg agggcctggc ctgccaccag
     1561 ctgtgcgccc gagggcactg ctggggtcca gggcccaccc agtgtgtcaa ctgcagccag
     1621 ttccttcggg gccaggagtg cgtggaggaa tgccgagtac tgcaggggct ccccagggag
     1681 tatgtgaatg ccaggcactg tttgccgtgc caccctgagt gtcagcccca gaatggctca
     1741 gtgacctgtt ttggaccgga ggctgaccag tgtgtggcct gtgcccacta taaggaccct
     1801 cccttctgcg tggcccgctg ccccagcggt gtgaaacctg acctctccta catgcccatc
     1861 tggaagtttc cagatgagga gggcgcatgc cagccttgcc ccatcaactg cacccactcc
     1921 tgtgtggacc tggatgacaa gggctgcccc gccgagcaga gagccagccc tctgacgggt
     1981 accgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca
     2041 gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc
     2101 acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg
     2161 gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg
     2221 taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac
     2281 aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc
     2341 aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc
     2401 aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg
     2461 gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac
     2521 tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag
     2581 gggaacgtct tctcatgctc cgtgatgcac gaggctctgc acaaccacta cacgcagaag
     2641 agcctctccc tgtctccggg taaataagct taagtttaaa ccgctgatca gcctcgactg
     2701 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     2761 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     2821 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2881 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     2941 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3001 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3061 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3121 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3181 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3241 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3301 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3361 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     3421 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3481 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3541 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3601 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3661 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     3721 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     3781 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3841 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3901 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3961 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4021 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4081 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4141 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4201 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4261 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4321 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4381 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4441 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4501 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4561 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4621 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4681 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4741 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     4921 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4981 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5041 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5101 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5761 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7081 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     7141 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     7201 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     7261 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     7321 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     7381 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7441 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7501 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7561 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7621 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7681 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7741 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7801 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7861 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     7921 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     7981 agacccaagc tggctagcgt ttaaacgggc cctctagact cgagcggccg ccactgtgct
     8041 ggatatctgc ag
//
