LOCUS       Exported                6627 bp DNA     circular SYN 22-FEB-2022
DEFINITION  Soluble expression of human 41BB-Fc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..493
                     /product="4-1BB"
                     /label=4-1BB CDS
                     /note="homology to the receptors for TNF and NGF; human 
                     homolog of murine T-cell receptor 4-1BB protein; 
                     transmembrane protein"
                     /note="/Original 
                     Translation=MGNSCYNIVATLLLVLNFERTRSLQDPCSNCPAGTFCDNNRNQICSP
                     CPPNSFSSAGGQRTCDICRQCKGVFRTRKECSSTSNAECDCTPGFHCLGAGCSMCEQDC
                     KQGQELTKKGCKDCCFGTFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPADL
                     SPGASSVTPPAPAREPGHSPQIISFFLALTSTALLFLLFFLTLRFSVVKRGRKKLLYIF
                     KQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL"
                     /note="/modified by=mclal85p"
                     /note="color: #993366; direction: RIGHT"
                     /db_xref="GI:571321"
                     /protein_id="AAA53133.1"
     CDS             494..523
                     /label=Hinge
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #993366; direction: RIGHT"
     CDS             524..853
                     /label=CH2
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ca6502; direction: RIGHT"
     CDS             854..1174
                     /label=CH3
                     /note="/Transferred From=Human IgG1 Hinge CH2-CH3, 
                     muBTLAIg"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #993366; direction: RIGHT"
     polyA_signal    1208..1432
                     /label=bGH poly(A) signal polyA signal
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      1478..1906
                     /direction=RIGHT
                     /label=f1 ori rep origin
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     promoter        1920..2249
                     /label=SV40 promoter
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      2100..2235
                     /direction=RIGHT
                     /label=SV40 ori rep origin
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             2316..3110
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII) CDS
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3284..3405
                     /label=SV40 poly(A) signal polyA signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    3478..3494
                     /label=lac operator protein bind
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(3502..3532)
                     /label=lac promoter
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments: 1: 
                     3321 .. 3327 / #ffffff / -10 2: 3328 .. 3345 / #ffffff 3: 
                     3346 .. 3351 / #ffffff / -35"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3856..4441)
                     /direction=LEFT
                     /label=ori rep origin
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             complement(4612..5472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla CDS
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments: 1: 4431 .. 5222 / 
                     #ccffcc 2: 5223 .. 5291 / #ccffcc / signal sequence 
                     Cleavage site after base 5222"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5473..5577)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        5843..6222
                     /label=CMV enhancer
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        6223..6426
                     /label=CMV promoter
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6471..6489
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    6567..6571
                     /label=Kozak
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #00ee00; direction: RIGHT"
     CDS             6572..6631
                     /label=OPT IL2 SIGNAL SEQUENCE
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #ff7400; direction: RIGHT"
ORIGIN
        1 tagtttgcag gatccttgta gtaactgccc agctggtaca ttctgtgata ataacaggaa
       61 tcagatttgc agtccctgtc ctccaaatag tttctccagc gcaggtggac aaaggacctg
      121 tgacatatgc aggcagtgta aaggtgtttt caggaccagg aaggagtgtt cctccaccag
      181 caatgcagag tgtgactgca ctccagggtt tcactgcctg ggggcaggat gcagcatgtg
      241 tgaacaggat tgtaaacaag gtcaagaact gacaaaaaaa ggttgtaaag actgttgctt
      301 tgggacattt aacgatcaga aacgtggcat ctgtcgaccc tggacaaact gttctttgga
      361 tggaaagtct gtgcttgtga atgggacgaa ggagagggac gtggtctgtg gaccatctcc
      421 agccgacctc tctccgggag catcctctgt gaccccgcct gcccctgcga gagagccagg
      481 acactctccg caggacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg
      541 gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac
      601 ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa
      661 ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta
      721 caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg
      781 caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat
      841 ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga
      901 ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga
      961 catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc
     1021 cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag
     1081 gtggcagcag gggaacgtct tctcatgctc cgtgatgcac gaggctctgc acaaccacta
     1141 cacgcagaag agcctctccc tgtctccggg taaataagct taagtttaaa ccgctgatca
     1201 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1261 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1321 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1381 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     1441 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     1501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     1921 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1981 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2041 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2161 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     2221 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     2281 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     2341 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2401 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2461 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     2521 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2581 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2641 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2701 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2761 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2821 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     2881 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2941 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3001 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3061 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3121 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     3181 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     3241 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     3301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     3421 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3601 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4381 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4441 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4501 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4561 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5581 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     5641 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     5701 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     5761 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     5821 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     5881 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5941 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6001 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     6061 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     6121 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6181 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     6241 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     6301 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     6361 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     6421 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     6481 cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact cgagcggccg
     6541 ccactgtgct ggatatctgc agaattccac catgcgaagg atgcaacttc ttctgctcat
     6601 cgcactttcc ttggcgctgg tgacaaa
//
