LOCUS       Exported                6846 bp DNA     circular SYN 22-FEB-2022
DEFINITION  Soluble expression of human TF-Fc (his-tagged).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6846
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(4433..5390,1..4432)
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="This feature has 2 segments:
                        1: 4433 .. 5390 / #ffffff
                        2:    1 .. 4432 / #ffffff"
                     /organism="synthetic DNA construct"
     polyA_signal    32..256
                     /label=bGH poly(A) signal polyA signal
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      302..730
                     /direction=RIGHT
                     /label=f1 ori rep origin
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     promoter        744..1073
                     /label=SV40 promoter
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      924..1059
                     /direction=RIGHT
                     /label=SV40 ori rep origin
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             1140..1934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII) CDS
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2108..2229
                     /label=SV40 poly(A) signal polyA signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    2302..2318
                     /label=lac operator protein bind
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(2326..2356)
                     /label=lac promoter
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments: 1: 
                     3321 .. 3327 / #ffffff / -10 2: 3328 .. 3345 / #ffffff 3: 
                     3346 .. 3351 / #ffffff / -35"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(2680..3265)
                     /direction=LEFT
                     /label=ori rep origin
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
                     /note="color: #ffff00"
     CDS             complement(3436..4296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla CDS
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments: 1: 4431 .. 5222 / 
                     #ccffcc 2: 5223 .. 5291 / #ccffcc / signal sequence 
                     Cleavage site after base 5222"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4297..4401)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        4667..5046
                     /label=CMV enhancer
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        5047..5250
                     /label=CMV promoter
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5295..5313
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    5403..5407
                     /label=KOZAK
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #ffdd55"
     misc_feature    5408..5467
                     /label=OPT IL2 SIGNAL SEQUENCE
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #ff7400; direction: RIGHT"
     misc_feature    5468..6118
                     /label=s huF3 (TF)
                     /note="/created by=User"
                     /note="/modified by=Sarah"
                     /note="color: #ff1199; direction: RIGHT"
     misc_feature    6125..6154
                     /label=Hinge
                     /note="/created by=User"
                     /note="color: #a6acb3"
     misc_feature    6155..6484
                     /label=CH2
                     /note="/created by=User"
                     /note="color: #ca6502"
     misc_feature    6485..6805
                     /label=CH3
                     /note="/created by=User"
                     /note="color: #a6acb3"
     misc_feature    6806..6829
                     /label=OCT-HIS
                     /note="/created by=User"
                     /note="/modified by=mclal85p"
                     /note="color: #4096ee"
ORIGIN
        1 agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
       61 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      121 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
      181 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
      241 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
      301 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      901 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      961 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     1021 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     1081 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     1141 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1201 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1261 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1321 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1381 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     1441 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     1501 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     1561 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1621 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1681 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1741 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1801 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1861 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1921 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     1981 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     2041 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     2101 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2161 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2221 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     2281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3121 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     4441 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4501 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     4561 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     4621 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4681 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4741 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4801 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4861 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4921 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4981 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5041 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     5101 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     5161 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     5221 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     5281 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
     5341 gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt cggcctctga
     5401 ggccaccatg cgaaggatgc aacttcttct gctcatcgca ctttccttgg cgctggtgac
     5461 aaatagtact acaaatactg tggcagcata taatttaact tggaaatcaa ctaatttcaa
     5521 gacaattttg gagtgggaac ccaaacccgt caatcaagtc tacactgttc aaataagcac
     5581 taagtcagga gattggaaaa gcaaatgctt ttacacaaca gacacagagt gtgacctcac
     5641 cgacgagatt gtgaaggatg tgaagcagac gtacttggca cgggtcttct cctacccggc
     5701 agggaatgtg gagagcaccg gttctgctgg ggagcctctg tatgagaact ccccagagtt
     5761 cacaccttac ctggagacaa acctcggaca gccaacaatt cagagttttg aacaggtggg
     5821 aacaaaagtg aatgtgaccg tagaagatga acggacttta gtcagaagga acaacacttt
     5881 cctaagcctc cgggatgttt ttggcaagga cttaatttat acactttatt attggaaatc
     5941 ttcaagttca ggaaagaaaa cagccaaaac aaacactaat gagtttttga ttgatgtgga
     6001 taaaggagaa aactactgtt tcagtgttca agcagtgatt ccctcccgaa cagttaaccg
     6061 gaagagtaca gacagcccgg tagagtgtat gggccaggag aaaggggagt tcagagaagg
     6121 taccgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc
     6181 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
     6241 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
     6301 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
     6361 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
     6421 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
     6481 caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg aggagatgac
     6541 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
     6601 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
     6661 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
     6721 ggggaacgtc ttctcatgct ccgtgatgca cgaggctctg cacaaccact acacgcagaa
     6781 gagcctctcc ctgtctccgg gtaaacatca ccaccatcac catcatcact gaggcctgtc
     6841 aggcca
//
