LOCUS       Exported                5977 bp DNA     circular SYN 22-FEB-2022
DEFINITION  pSBbi vector with blasticidin resistance and ametrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..2635,3390..5977)
                     /mol_type="other DNA"
                     /note="This feature has 2 segments:
                        1:    1 .. 2635 / #ffffff
                        2: 3390 .. 5977 / #ffffff"
                     /organism="synthetic DNA construct"
     source          2633..2635
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          2638..2642
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          3363..3392
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     regulatory      794..803
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #ffffff; direction: RIGHT"
                     /regulatory_class="other"
     promoter        complement(829..2010)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation  
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: LEFT"
     promoter        2018..2629
                     /label=RPBSA
                     /note="/created by=Sarah"
                     /note="/modified by=Sarah"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2638..2642
                     /label=Kozac
                     /note="Geneious type: motif"
                     /note="/created by=Aarati"
                     /note="color: #a6acb3; direction: RIGHT"
     gene            2643..3362
                     /label=mAmetrine
                     /note="/created by=Microbiology"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3380..3680
                     /label=rabbit beta-globin polyadenylation signal
                     /note="color: #cc0000; direction: RIGHT; rBG-pA; rabbit 
                     beta-globin polyadenylation signal"
                     /note="/Transferred From=1F5-CD20-Mutated SS - in 
                     SBi-RPStrep-tag, B3F6 CAR IL2SS ideal with Sfi EcoRI kpnI 
                     in pSB-RP, B3F6 CAR in pSB-RP, B3F6 CAR in pSB-RP IL2SS 
                     ideal, CR1 in pSBbi-GP (reversed), Digestion of CR1 in 
                     pSBbi-GP (reversed) - Fragment 2, Her2 CAR in SBi-RP, 
                     pSBbi-RP, pSBbi-RP (modified), pSBi-RP + scfv Cr1, tag+vec,
                     tagless+vector-tag"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=B3F6 CAR in pSB-RP; pSBbi-RP 
                     (modified)"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/modified by=Sarah"
                     /note="color: #6bba70; direction: RIGHT"
     polyA_site      3527..3582
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(4145..4733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     CDS             complement(4904..5764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5765..5869)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca
      301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gaggctgatc agcgagctct agagaattga tccccaagct tggcctgaca
      601 ggccttcgaa ttagagagaa ggaaagacgc aaccaggaag cccaccaaga gaaagaggca
      661 tttcctgaaa agattcccct ttttggagag ccctacaagg tatttactga acactagaca
      721 ttgaagtcct gatttatcac aatgttgaac cctatgatga aacaattcag tataattgag
      781 ttcgaaactc gagccatggt ggcctcagag gccagcttgg ggtagttttc acgacacctt
      841 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
      901 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
      961 ctccacccac tcagtatggg gaaactccat cgcataaaac ccctcccccc aacctaaaga
     1021 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
     1081 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
     1141 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg
     1201 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg
     1261 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta
     1321 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg
     1381 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc
     1441 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa
     1501 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat
     1561 tcgcacgcgg cggccccagc gcccaggcca agcctcaact caagcacgag gcgaaggggc
     1621 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag
     1681 aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg
     1741 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     1801 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     1861 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     1921 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     1981 tgcgctctgc ccactgacgg gcaccggagc ctctaggcga gaccctgtct cacaaaataa
     2041 agtaagcccg gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc
     2101 caccagaggc cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac
     2161 atggcggaca gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct
     2221 ggacggccgt gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag
     2281 cgctgaccgc ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg
     2341 ccttccctgg ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag
     2401 ccgtccgcgc aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta
     2461 gacggaactt cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt
     2521 tcctgcgttg ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc
     2581 acagcaaact aactgtagcc tttctctctt tccctgtaga aacctctgca cctgaggcca
     2641 ccatggtctc taagggtgaa gagcttttca ctggtgtggt cccaatcctg gtagaattgg
     2701 acggcgatgt caacggccat agattcagcg tccgcgggga gggtgaaggg gacgccacaa
     2761 atggcaagct gacgttgaag tttatttgca catcaggaga attgccggtc ccctggccga
     2821 cattggtgac gacgctttcc tatggagtgc agtgctttgc tcgataccca gatcacatga
     2881 aacagcatga cttttttaag tcagcgatgc ccgagggcta cgtgcaagag cggacaatat
     2941 cttttaagga cgatggaagt tacagaacaa gggcggaagt caagttcgag ggggacacct
     3001 tggttaatcg gatagagctg aaaggcatcg acttcaagga ggacggtaat atccttggcc
     3061 ataagttgga atataacatg aatgcgtggg atgcttacat cacagcggac aaacagaaga
     3121 acggcataaa agccaatttc aagatagagc acaatgtaaa ggacggaggt gtacaattgg
     3181 ctgacgcgta ccagcaaaac acccccattg gggacgggtc cgtattgctc ccagataatc
     3241 attacctttc ttttcaatct aaattgttca aggaccccaa tgagcaaagg gatcatatgg
     3301 tactcctgga atttgtgaca gcagccggta taacccctgg tatggacgaa ctctataagt
     3361 aattcgaagg cctgtcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     3421 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     3481 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3541 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     3601 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     3661 atatgctggc tgccatgaac tagctactcg ggacccctta ccgaaacatc gccgcattct
     3721 gcagaggagt cgagtgtatg taaacttctg acccactggg aatgtgatga aagaaataaa
     3781 agctgaaatg aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt
     3841 ggtgatccta actgacctaa gacagggaat ttttactagg attaaatgtc aggaattgtg
     3901 aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa acttccgact tcaactgtat
     3961 agggatccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4021 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4081 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4801 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5881 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5941 atcaggaaat tgtaagcgtt aatattttgt taaaatt
//
