LOCUS       CROP-C9-Puro_JAK3      11691 bp ds-DNA     circular     24-FEB-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct.                                           
FEATURES             Location/Qualifiers
     source          1..11691
                     /label="source:synthetic DNA construct"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     1..17
                     /label="In lacZ gene. Also called M13-rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     1..17
                     /label="common sequencing primer, one of multiplesimilar  variants"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     36..56
                     /label="T3 promoter, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        38..56
                     /label="promoter for bacteriophage T3 RNApolymerase"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     promoter        84..310
                     /label="Rous sarcoma virus enhancer/promoter"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     LTR             311..491
                     /label="truncated 5' long terminal repeat (LTR)from HIV-1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    538..663
                     /label="packaging signal of human immunodeficiencyvirus  type 1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1156..1389
                     /label="The Rev response element (RRE) of HIV-1allows for Rev-dependent mRNA export from the nucleus tothe"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1799..1916
                     /label="central polypurine tract and centraltermination  sequence of HIV-1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2105..2316
                     /label="core promoter for human elongation factorEF-1-alpha"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     regulatory      2335..2344
                     /label="vertebrate consensus sequence for stronginitiationof translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2341..6444
                     /label="Cas9 (Csn1) endonuclease from theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            2341..6444
                     /label="Translation 2341-6444"
     CDS             6445..6492
                     /label="bipartite nuclear localization signal fromnucleoplasmin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            6445..6492
                     /label="Translation 6445-6492"
     CDS             6493..6516
                     /label="FLAG(R) epitope tag, followed by anenterokinasecleavage site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            6493..6516
                     /label="Translation 6493-6516"
     CDS             6526..6582
                     /label="2A peptide from porcine teschovirus-1polyprotein"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            6526..6582
                     /label="Translation 6526-6582"
     misc_feature    6583..7181
                     /label="GTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCTGGCGAGGGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             6583..7182
                     /label="PuroR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            6583..7182
                     /label="Translation 6583-7182"
     misc_feature    7198..7786
                     /label="woodchuck hepatitis virusposttranscriptional  regulatory element"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(7251..7271)
                     /label="WPRE, reverse primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(7669..7680)
                     /label="Factor Xa recognition and cleavage site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(7669..7680)
                     /label="Translation 7680-7669"
     primer_bind     7942..7962
                     /label="Human U6 promoter, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        7942..8182
                     /label="RNA polymerase III promoter for human U6snRNA"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     primer_bind     8113..8132
                     /label="Human U6 promoter, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8192..8211
                     /label="CRISPR_guide_insert"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_RNA        8212..8287
                     /label="guide RNA scaffold for the Streptococcuspyogenes  CRISPR/Cas9 system"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     LTR             8340..8520
                     /label="truncated 5' long terminal repeat (LTR)from HIV-1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    8598..8719
                     /label="SV40 polyadenylation signal"
                     /ApEinfo_revcolor=#ff3eee
                     /ApEinfo_fwdcolor=#ff3eee
     primer_bind     complement(8635..8654)
                     /label="SV40 polyA, reverse primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     8689..8708
                     /label="SV40 polyA terminator, reverse primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      8759..8894
                     /label="rep_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     8821..8840
                     /label="SV40 promoter/origin, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(8914..8933)
                     /label="T7 promoter, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(8915..8933)
                     /label="promoter for bacteriophage T7 RNApolymerase"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     primer_bind     complement(8943..8959)
                     /label="common sequencing primer, one of multiplesimilar  variants"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(8943..8960)
                     /label="In lacZ gene. Also called M13-F20 or M13(-21)  Forward"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(8952..8974)
                     /label="In lacZ gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      9101..9556
                     /label="rep_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(9188..9207)
                     /label="F1 origin, reverse primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     9398..9419
                     /label="F1 origin, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        9582..9686
                     /label="bla"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     CDS             9687..10547
                     /label="beta-lactamase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            9687..10547
                     /label="Translation 9687-10547"
     primer_bind     complement(9905..9924)
                     /label="Ampicillin resistance gene, reverseprimer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      10718..11306
                     /label="rep_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     11207..11226
                     /label="pBR322 origin, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     11460..11477
                     /label="L4440 vector, forward primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    11594..11615
                     /label="CAP binding activates transcription in thepresenceof cAMP."
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        11630..11660
                     /label="promoter for the E. coli lac operon"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     protein_bind    11668..11684
                     /label="The lac repressor binds to the lacoperator to  inhibit transcription in E. coli. Thisinhibition ca"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     join(11673..11691,1..4)
                     /label="In lacZ gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
       61 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      121 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      181 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      241 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      301 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
      721 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      781 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      841 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
      901 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
      961 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1021 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1081 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1141 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1201 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1261 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1321 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1381 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1441 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1501 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1561 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1621 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1681 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1741 gggatattca ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1921 tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa
     1981 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
     2041 aaggagtggg aattggctcc gtaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2101 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2161 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2221 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2281 gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag cgctgccacc
     2341 atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
     2401 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     2461 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     2521 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     2581 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     2641 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     2701 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     2761 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     2821 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     2881 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     2941 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     3001 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     3061 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     3121 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     3181 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     3241 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     3301 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     3361 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     3421 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     3481 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     3541 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     3601 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     3661 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     3721 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     3781 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     3841 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     3901 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     3961 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     4021 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     4081 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     4141 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     4201 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     4261 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     4321 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     4381 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     4441 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     4501 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     4561 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     4621 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     4681 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     4741 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     4801 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     4861 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     4921 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     4981 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     5041 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     5101 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     5161 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     5221 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     5281 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     5341 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     5401 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     5461 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     5521 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     5581 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     5641 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     5701 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     5761 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     5821 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     5881 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     5941 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     6001 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     6061 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     6121 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     6181 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     6241 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     6301 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     6361 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     6421 gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc tggacaggct
     6481 aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
     6541 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatgaccga gtacaagccc
     6601 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6661 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6721 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6781 gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6841 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6901 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6961 accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7021 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7081 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7141 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat
     7201 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7261 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7321 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7381 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7441 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7501 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7561 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7621 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7681 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7741 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     7801 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7861 aagggctaat tcactcccaa cgaagacaag atttcaccat tatcgtttca gacccacctc
     7921 ccaaccccga ggggacccag agagggccta tttcccatga ttccttcata tttgcatata
     7981 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
     8041 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     8101 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     8161 ttatatatct tgtggaaagg acgaaacacc gGGAAGCTGT CAAAGTCCTG Ggttttagag
     8221 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     8281 tcggtgcttt tttaagcttg gcgtaactag atcttgagac actgcttttt gcttgtactg
     8341 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8401 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8461 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8521 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8581 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8701 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8761 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8821 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8881 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8941 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9001 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9061 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9121 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9181 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9241 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9301 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9361 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11461 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11521 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    11581 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11641 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac a
//