LOCUS       Exported                6615 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    209..863
                     /label=pCMV
                     /note="pCMV"
     misc_feature    900..930
                     /label=H2BE-for
                     /note="H2BE-for"
     misc_feature    900..909
                     /label=Kpn1-H2B
                     /note="Kpn1-H2B"
     misc_feature    901..906
                     /label=Kozak
                     /note="Kozak"
     misc_feature    907..1302
                     /label=H2BR1mut-mCherry CDS
                     /note="H2BR1mut-mCherry CDS"
     CDS             907..1284
                     /label=HsH2B.1R1mut
                     /note="HsH2B.1R1mut"
     misc_feature    1087..1107
                     /label=H2B-delR1-for
                     /note="H2B-delR1-for"
     misc_feature    complement(1087..1107)
                     /label=H2B-delR1-rev-RC
                     /note="H2B-delR1-rev-RC"
     misc_feature    1088..1100
                     /label=BspH1 blocked by dam methylation
                     /note="BspH1 blocked by dam methylation"
     misc_feature    1096..1098
                     /label=mutated from AAT
                     /note="mutated from AAT"
     misc_feature    1139..1158
                     /label=HN941
                     /note="HN941"
     misc_feature    complement(1264..1289)
                     /label=H2BE-rev-RC
                     /note="H2BE-rev-RC"
     misc_feature    complement(1282..1302)
                     /label=H2B-rev-RC
                     /note="H2B-rev-RC"
     misc_feature    1294..1302
                     /label=H2B-mCherry-for
                     /note="H2B-mCherry-for"
     misc_feature    1303..2016
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    1303..1333
                     /label=Untitled
     misc_feature    complement(1990..2016)
                     /label=Untitled
     misc_feature    2017..2028
                     /label=mGRASP T2A
                     /note="mGRASP T2A"
     misc_feature    2029..2106
                     /label=mGRASP T2A
                     /note="mGRASP T2A"
     misc_feature    complement(2029..2071)
                     /label=HN791
                     /note="HN791"
     misc_feature    2052..2129
                     /label=HN792
                     /note="HN792"
     misc_feature    2107..3162
                     /label=iCre
                     /note="iCre"
     misc_feature    complement(3144..3170)
                     /label=HN793
                     /note="HN793"
     gene            3175..3477
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    3303..3322
                     /label=451-mod1-for
                     /note="451-mod1-for"
     misc_feature    3370..3389
                     /label=Untitled
     misc_feature    3455..3477
                     /label=Untitled
     misc_feature    3478..3488
                     /label=3x STOP
                     /note="3x STOP"
     misc_feature    3489..3496
                     /label=Untitled
     misc_feature    3497..3501
                     /label=Untitled
     misc_feature    4801..5474
                     /label=ColE1
                     /note="ColE1"
     misc_feature    5619..6479
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gccaccatgc ctgaaccctc taagtctgct ccagccccta aaaagggttc taagaaggct
      961 atcactaagg cgcagaagaa ggatggtaag aagcgtaagc gcagccgcaa ggagagctat
     1021 tctatctatg tgtacaaggt tctgaagcag gtccaccccg acaccggcat ctcatccaag
     1081 gccatgggga tcatgaactc cttcgtcaac gacatcttcg agcgcatcgc gggcgaggct
     1141 tctcgcctgg ctcactacaa taagcgctcg accatcacct ccagggagat tcagacggct
     1201 gtgcgcctgc tgctgcctgg ggagctggct aagcatgctg tgtccgaggg cactaaggca
     1261 gttaccaagt acactagctc taaggatcca ccggtcgcca ccatgagcaa aggagaagaa
     1321 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1381 ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac ccttaaattt
     1441 atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac tctgacctat
     1501 ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt tttcaagagt
     1561 gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga cgggacctac
     1621 aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaag
     1681 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta caactttaac
     1741 tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
     1801 attcgccaca acgttgaaga tggttccgtt caactagcag accattatca acaaaatact
     1861 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgtc
     1921 ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct
     1981 gctgggatta cacatggcat ggatgagctc tacaaaggcg gctccggcgg ccgctccgag
     2041 ggccgcggct ccctgctgac ctgcggcgac gtggaggaga accccggccc cgcccccggc
     2101 tccaccatgg tggtgcccaa gaagaagagg aaagtctcca acctgctgac tgtgcaccaa
     2161 aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac
     2221 atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc
     2281 agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag
     2341 gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa
     2401 cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac
     2461 tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag
     2521 agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg
     2581 gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac
     2641 accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat
     2701 ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg
     2761 gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt
     2821 gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc
     2881 ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac
     2941 cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac
     3001 tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc
     3061 atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac
     3121 tctgagactg gggccatggt gaggctgctc gaggatgggg actgataagg cgcgttagag
     3181 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3241 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3301 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3361 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3421 tggcttctga ggcggaaaga accagctggg gctcgactag agcttgcgga acccttctaa
     3481 ctagataagg cgcgccctgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3541 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3601 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     3661 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     3721 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     3781 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     3841 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     3901 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     3961 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4021 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4081 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4141 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4201 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4261 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     4321 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4381 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     4441 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4501 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4561 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4621 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5041 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5281 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6601 gtgccacctg acgtc
//
