LOCUS       Exported                7097 bp DNA     circular SYN 02-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     misc_feature    156..1448
                     /label=a-CaMKII(1.3)
                     /note="/vntifkey=21"
     misc_feature    1487..2413
                     /label=ChRmine
                     /note="/vntifkey=21"
     misc_feature    1583..1585
                     /label=H33R
                     /note="/vntifkey=21"
     misc_feature    2423..2482
                     /label=TS
                     /note="/vntifkey=21"
     misc_feature    2483..3175
                     /label=mScarlet
                     /note="/vntifkey=21"
     misc_feature    2762..2764
                     /label=E95D
                     /note="/vntifkey=21"
     enhancer        3176..3196
                     /label=ER export
                     /label=ER\export
                     /note="/vntifkey=9"
     misc_feature    3214..3822
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3842..4320
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    4360..4500
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4592..4898
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5417..6274
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6425..7092
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccggatcccc gggtaccggt gccaccatgg cacacgcacc
     1501 aggcaccgac cagatgttct acgtgggcac aatggacggc tggtatctgg ataccaagct
     1561 gaactccgtg gccatcggcg ccaggtggtc ttgctttatc gtgctgacaa tcaccacatt
     1621 ctacctgggc tatgagtcct ggacatctcg gggccctagc aagagaacct ccttttacgc
     1681 cggctatcag gaggagcaga acctggccct gtttgtgaat ttctttgcca tgctgagcta
     1741 cttcggcaag atcgtggccg acacactggg ccacaacttc ggcgatgtgg gccccttcat
     1801 catcggcttc ggcaattaca ggtatgccga ctacatgctg acctgcccaa tgctggtgta
     1861 cgatctgctg tatcagctga gggcccccta tcgcgtgtct tgtagcgcca tcatctttgc
     1921 catcctgatg tctggcgtgc tggccgagtt ctacgcagag ggcgacccta ggctgaggaa
     1981 tggcgcctac gcctggtatg gctttggctg tttctggttt atcttcgcct actctatcgt
     2041 gatgagcatc gtggccaagc agtatagccg gctggcccag ctggcccagg atacaggcgc
     2101 cgagcacagc ctgcacgtgc tgaagttcgc cgtgtttacc ttctccatgc tgtggattct
     2161 gtttcccctg gtgtgggcca tctgccctag aggcttcggc tggatcgacg ataactggac
     2221 agaggtggcc cactgcgtgt gcgacatcgt ggccaagtcc tgttacggct ttgccctggc
     2281 ccggttcaga aagacctatg atgaggagct gtttcggctg ctggagcagc tgggacacga
     2341 cgaggatgag ttccagaagc tggagctgga tatgaggctg agcagcaatg gcgagcgcct
     2401 gcggagactg tctgcggccg ccaagagcag gatcaccagc gagggcgagt acatccccct
     2461 ggaccagatc gacatcaacg tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg
     2521 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     2581 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     2641 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac
     2701 caagcacccc gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg
     2761 ggaccgcgtg atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct
     2821 ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg
     2881 ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga
     2941 ggacggcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcgggcgcta
     3001 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta
     3061 caacgtggac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca
     3121 gtacgaacgc tccgagggcc gccactccac cggcggcatg gacgagctgt acaagttctg
     3181 ctacgagaac gaggtgtaag aattcgatat caagcttatc gataatcaac ctctggatta
     3241 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3301 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3361 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3421 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3481 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3541 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3601 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3661 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3721 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3781 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     3841 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     3901 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     3961 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4021 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4081 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4141 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4201 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4261 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4321 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     4381 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4441 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     4501 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     4561 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4621 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     4801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     4921 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4981 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7081 gccttttgct cacatgt
//
