LOCUS       pAAV-Ef1a-DIO\rsChRmine-oScarlett-WPRE        7293 bp    DNA     circular      8-FEB-2022
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14255284|
COMMENT     VNTDBDATE|-14255284|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-Ef1a-DIO rsChRmine-oScarlett-WPRE|
COMMENT     VNTAUTHORNAME|Charu|
COMMENT     VNTAUTHOREML|charur@stanford.edu|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-Ef1a-DIO rsChRmine-oScarlett-WPRE" 0 7293 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" 
COMMENT         "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaII" "AvrII" "BaeI" "BanI" "BanII" "BbeI" "Bbr7I" "BbsI" "BbvCI" 
COMMENT         "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" "BplI" "BpmI" 
COMMENT         "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" "BsaWI" "BsaXI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" 
COMMENT         "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" "Bsp24I" "BspCNI" 
COMMENT         "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" "BsrGI" "BsrI" "BssHII" 
COMMENT         "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" 
COMMENT         "ChaI" "CjeI" "CjePI" "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" 
COMMENT         "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" 
COMMENT         "EcoNI" "EcoO109I" "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" 
COMMENT         "HaeIV" "HgaI" "HhaI" "Hin4I" "HincII" "HinfI" "HinP1I" "HpaI" "HpaII" 
COMMENT         "HphI" "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4III" "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" 
COMMENT         "MaeIII" "MboI" "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" 
COMMENT         "MseI" "MslI" "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NdeI" "NgoMIV" 
COMMENT         "NheI" "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" 
COMMENT         "PciI" "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" 
COMMENT         "PshAI" "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" "Sse232I" 
COMMENT         "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" "StyI" 
COMMENT         "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" "Tsp45I" 
COMMENT         "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" "UnbI" "UthSI" 
COMMENT         "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7293 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 5 2222 0 3630 0 5232 0 
COMMENT            5729 0 6975 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 15 41 0 52 0 288 0 390 0 
COMMENT            575 0 1090 0 1207 0 1814 0 2006 0 3103 0 4027 0 4332 0 4642 0 4653 0 
COMMENT            5109 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1421 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 3416 0 4011 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3397 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 157 0 3409 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3265 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 7 0 558 0 1063 0 4695 0 0 
COMMENT            "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 4644 0 4655 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 4642 0 4653 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 3874 0 5482 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AceIII" "cagctc" 14 18 7 1598 0 2399 0 2456 0 
COMMENT             3697 0 5158 1 5355 1 6097 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AciI" "ccgc" 2 4 88 38 0 143 1 147 0 343 1 
COMMENT             380 0 460 0 494 0 496 1 676 0 679 0 800 1 840 0 842 1 846 1 889 1 
COMMENT             904 1 971 0 986 0 995 1 1031 1 1043 0 1084 1 1099 1 1103 1 1599 0 
COMMENT             1613 1 1682 1 1747 0 1761 0 1826 0 1896 0 1898 1 1965 0 1988 1 2043 
COMMENT             1 2142 1 2165 1 2234 0 2334 1 2338 0 2351 0 3730 1 3743 0 3756 0 
COMMENT             3866 1 3907 1 3921 0 3923 1 3942 1 3952 0 3998 0 4006 0 4234 1 4294 
COMMENT             0 4540 1 4549 1 4553 0 4658 1 4708 1 4734 1 4747 0 4784 1 4798 1 
COMMENT             4801 1 4829 0 4856 0 5261 0 5287 0 5297 0 5336 0 5510 1 5557 0 5656 
COMMENT             1 5765 1 5842 1 5886 0 6007 1 6053 0 6244 1 6335 1 6697 0 6706 1 
COMMENT             6841 0 6951 1 7072 1 7091 1 7218 1 7246 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 436 0 5215 0 5799 0 6172 
COMMENT             0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 7 1311 0 1754 1 1784 1 
COMMENT             2117 1 2615 1 5735 0 6747 1 0 "") 
COMMENT        #18=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 4022 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 621 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflIII" "acrygt" 2 0 3 151 0 875 0 7289 0 0 
COMMENT             "") #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 321 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 5 71 0 2093 0 4154 0 
COMMENT             4628 0 6401 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 2 1644 0 4098 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 2 4516 0 5041 1 0 
COMMENT             "") 
COMMENT        #25=(CRSite (CStringList) "AluI" "agct" 3 0 38 12 0 159 0 205 0 580 0 
COMMENT             938 0 1068 0 1212 0 1304 0 1472 0 1483 0 1588 0 1903 0 2137 0 2389 
COMMENT             0 2395 0 2425 0 2446 0 2674 0 2747 0 2878 0 3017 0 3196 0 3411 0 
COMMENT             3687 0 4255 0 4372 0 4686 0 4915 0 5172 0 5350 0 5369 0 6048 0 6111 
COMMENT             0 6211 0 6732 0 6989 0 7035 0 7125 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AlwI" "ggatc" 10 11 15 566 1 1360 0 1416 1 
COMMENT             1429 0 2315 1 2543 1 3991 0 5762 0 5766 1 6083 0 6546 1 6547 0 6643 
COMMENT             1 6645 0 6731 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 2398 0 3055 0 6880 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 2 1023 0 2648 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ApaI" "gggccc" 6 0 4 859 0 2873 0 2965 0 3103 
COMMENT             0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ApoI" "raatty" 2 0 7 745 0 1331 0 3033 0 3397 
COMMENT             0 3440 0 5185 0 5196 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1546 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "AseI" "attaat" 3 0 1 6226 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 8 211 0 1684 0 1990 0 2290 
COMMENT             0 3909 0 4542 0 6037 0 6259 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 2783 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 19 249 0 324 0 696 0 955 0 
COMMENT             1015 0 1180 0 1191 0 1697 0 1892 0 1922 0 1961 0 2150 0 2654 0 2768 
COMMENT             0 3173 0 3248 0 4698 0 4961 0 6448 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BanII" "grgcyc" 6 0 9 631 0 859 0 1070 0 2215 
COMMENT             0 2873 0 2965 0 3103 0 3774 0 4931 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 7 1184 0 1701 0 1965 0 
COMMENT             2658 0 2772 0 3177 0 4702 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 3 1842 1 3944 1 4226 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 1847 1 3949 1 4221 0 0 
COMMENT             "") 
COMMENT        #42=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 4 1144 0 2778 0 2874 0 
COMMENT             4324 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BbvI" "gcagc" 14 18 26 21 0 214 0 540 1 703 1 
COMMENT             748 1 1045 1 1470 1 2356 1 2412 1 2446 0 3489 1 3745 1 3821 1 3917 
COMMENT             1 4010 1 4673 1 4695 0 4832 0 5356 1 5967 1 6358 0 6661 1 6867 1 
COMMENT             6870 1 6960 0 7292 1 0 "") 
COMMENT        #44=(CRSite (CStringList) "BccI" "ccatc" 10 11 14 133 0 163 1 1032 1 
COMMENT             1252 1 1791 0 2567 1 2837 1 3173 1 4564 1 4987 1 5019 0 5944 1 6231 
COMMENT             1 6355 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BceAI" "acggc" 18 20 19 414 1 465 1 958 1 
COMMENT             1133 1 1168 1 1748 1 1793 1 1859 1 1910 1 1952 1 1975 0 2054 1 2186 
COMMENT             1 2471 1 2638 0 3200 1 3743 0 4975 0 6789 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BcefI" "acggc" 18 19 19 415 1 466 1 959 1 
COMMENT             1134 1 1169 1 1749 1 1794 1 1860 1 1911 1 1953 1 1975 0 2055 1 2187 
COMMENT             1 2472 1 2638 0 3201 1 3743 0 4975 0 6790 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 4335 0 5863 1 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 7 1434 1 2672 0 3212 0 
COMMENT             3305 0 3490 1 5564 0 7091 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 2248 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BfaI" "ctag" 2 0 14 131 0 218 0 329 0 734 0 
COMMENT             2565 0 2784 0 3096 0 3274 0 4151 0 4565 0 4849 0 6208 0 6543 0 6796 
COMMENT             0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 4357 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 9 43 0 1013 0 1189 0 
COMMENT             1306 0 2567 0 2676 0 3270 0 4658 0 6283 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BglII" "agatct" 2 0 2 808 0 4033 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 3 2373 0 3018 0 4368 0 0 
COMMENT             "") 
COMMENT        #55=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 14 254 0 859 0 2155 0 
COMMENT             2226 0 2873 0 2965 0 3103 0 3229 0 3634 0 3787 0 4105 0 5236 0 5733 
COMMENT             0 6979 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BmrI" "actggg" 12 11 4 362 0 2980 1 4059 1 
COMMENT             6361 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 3277 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 4042 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BpmI" "ctggag" 23 21 7 1916 1 2085 0 2375 1 
COMMENT             2414 1 4081 1 4277 0 6332 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 4 1144 0 2778 0 2874 0 
COMMENT             4324 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 7 666 0 918 1 1352 1 
COMMENT             5797 0 6665 0 6906 1 7204 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 562 0 2638 0 3232 0 
COMMENT             4537 0 5002 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2283 0 2709 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 12 57 0 1181 0 1698 0 
COMMENT             1962 0 2655 0 2769 0 3174 0 3871 0 4639 0 4699 0 5479 0 5861 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 4430 0 6335 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 38 41 0 52 0 391 0 494 0 
COMMENT             662 0 840 0 989 0 990 0 1167 0 1326 0 1618 0 1642 0 1813 0 1896 0 
COMMENT             1933 0 1945 0 2005 0 2062 0 2098 0 2122 0 2293 0 2476 0 2584 0 2665 
COMMENT             0 2783 0 3126 0 3184 0 3265 0 3725 0 3797 0 3829 0 3921 0 4081 0 
COMMENT             4300 0 4458 0 4642 0 4653 0 7129 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 5 246 0 321 0 6105 0 6936 
COMMENT             0 7083 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 4 1614 1 1656 1 
COMMENT             4514 0 5041 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BscAI" "gcatc" 10 12 13 1713 0 2698 1 4016 0 
COMMENT             4054 0 4143 0 4698 1 4734 0 5250 1 5340 0 5703 1 5948 0 6143 1 7195 
COMMENT             1 0 "") 
COMMENT        #71=(CRSite (CStringList) "BseMII" "ctcag" 16 14 21 23 1 98 0 1158 0 
COMMENT             1266 1 1388 0 2072 1 2387 0 2792 0 2888 0 3032 0 3985 0 4338 0 4382 
COMMENT             0 4455 0 4602 1 4677 0 5253 0 5914 0 6431 1 6597 1 7006 1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 2438 1 3050 1 3546 1 
COMMENT             3607 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BseYI" "cccagc" 2 6 7 669 1 867 0 1843 0 2421 
COMMENT             0 2674 1 4475 1 6985 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 439 0 797 1 1694 1 
COMMENT             2660 0 4282 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 147 0 1013 0 2338 0 
COMMENT             4553 0 5883 0 6032 0 6955 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 8 1070 0 2226 0 2652 0 
COMMENT             3634 0 5236 0 5733 0 5818 0 6979 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 50 59 0 131 0 259 0 
COMMENT             260 0 334 0 396 0 397 0 512 0 582 0 663 0 917 0 990 0 991 0 1049 0 
COMMENT             1061 0 1122 0 1660 0 1681 0 1815 0 1832 0 1881 0 2105 0 2160 0 2161 
COMMENT             0 2330 0 2398 0 2477 0 2584 0 2585 0 2606 0 2765 0 2905 0 3611 0 
COMMENT             3662 0 3761 0 3830 0 3922 0 3933 0 4082 0 4300 0 4570 0 4642 0 4783 
COMMENT             0 5109 0 5110 0 5363 0 6811 0 7090 0 7256 0 7274 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 12 716 0 735 0 936 0 1280 
COMMENT             1 2352 0 3587 0 4388 1 4430 0 5364 0 5406 1 5559 1 6335 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 4388 1 5364 0 5406 1 0 
COMMENT             "") 
COMMENT        #81=(CRSite (CStringList) "BsmFI" "gggac" 16 20 9 270 1 850 1 1976 1 
COMMENT             2081 1 2100 0 2404 1 3711 0 3871 1 3882 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 21 254 0 631 0 859 0 
COMMENT             1070 0 2155 0 2215 0 2226 0 2652 0 2873 0 2965 0 3103 0 3229 0 3634 
COMMENT             0 3774 0 3787 0 4105 0 4931 0 5236 0 5733 0 5818 0 6979 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 6 2110 1 2529 1 
COMMENT             3666 1 5501 0 6595 1 6773 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 21 24 1 97 0 1157 0 
COMMENT             1267 1 1387 0 2073 1 2386 0 2791 0 2887 0 3031 0 3984 0 4337 0 4381 
COMMENT             0 4454 0 4603 1 4676 0 5252 0 5913 0 6432 1 6598 1 7007 1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 5456 0 5561 0 6569 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 766 0 2900 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 6 1031 1 1099 1 1828 0 
COMMENT             4549 1 4858 0 5559 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 2364 1 3668 1 6167 0 
COMMENT             6341 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 8 321 0 942 0 1601 0 2683 
COMMENT             0 3071 0 3933 0 4897 0 6316 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1582 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BsrI" "actgg" 7 5 21 357 0 378 0 554 0 823 0 
COMMENT             1019 1 2537 1 2986 1 3167 1 3462 0 3663 0 4065 1 4098 1 5091 0 5756 
COMMENT             0 5926 1 6195 0 6238 0 6356 0 6762 0 6874 1 6887 1 0 "") 
COMMENT        #94=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1546 0 4594 
COMMENT             0 4679 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 639 1 2195 1 5425 1 
COMMENT             5732 0 7116 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 2 1022 0 2647 0 0 
COMMENT             "") #97=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 2 2126 0 4529 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 9 2041 0 2098 0 2305 0 
COMMENT             2406 1 4046 1 5332 1 5955 0 6242 0 6423 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstKTI" "gatc" 4 0 28 574 0 811 0 1355 0 
COMMENT              1424 0 1918 0 2188 0 2251 0 2287 0 2323 0 2551 0 2892 0 3004 0 
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COMMENT                              "BplI: 1 site") 38 1 
COMMENT                             "NNNNNNNNNNNNNNGAGNNNNNCTCNNNNNNNNNNNNNN" 
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COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                             "ACNNNNNCTCCNNNNNNNNNNN" "TGNNNNNGAGGNNNNNNNNNNN") 
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COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
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COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
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COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
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COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
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COMMENT                             "GACNNNNNNTGGNNNNNNNNNNNNN" 
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COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
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COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
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COMMENT                              "BstXI: 1 site") 8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                       #508=(CRSFolderPar 
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COMMENT                       #510=(CRSFolderPar 
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COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
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COMMENT                       #513=(CRSFolderPar 
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COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
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COMMENT                               (CParagraph 117702584 (8 0) 1 1 1 0 178) 
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COMMENT                             "GACNNNNNNGTC" "CTGNNNNNNCAG") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     polyA_signal    4038..4516
                     /vntifkey="25"
                     /label=hGH\polyA
     misc_feature    4556..4696
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      4788..5094
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    5613..6470
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6621..7288
                     /vntifkey="33"
                     /label=pUC\ori
     promoter        156..1419
                     /vntifkey="29"
                     /label=EF-1a
     misc_feature    3410..4018
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    1426..1459
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    complement(3278..3311)
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    1510..1543
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(3362..3395)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(2272..2331)
                     /vntifkey="21"
                     /label=TS
     enhancer        complement(1558..1578)
                     /vntifkey="9"
                     /label=ER\export
     misc_feature    complement(1579..2271)
                     /vntifkey="21"
                     /label=mScarlet
     misc_feature    1990..1992
                     /vntifkey="21"
                     /label=E95D
     misc_feature    complement(2830..2832)
                     /vntifkey="21"
                     /label=I146M
     misc_feature    complement(2746..2748)
                     /vntifkey="21"
                     /label=G174S
     misc_feature    complement(2341..3267)
                     /vntifkey="21"
                     /label=ChRmine
BASE COUNT     1501 a      1971 c      2013 g      1808 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg 
     1441 atactttata cgaagttatg cagaatggta gctggattgt agctgctatt agcaatatga 
     1501 aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc cctattacac 
     1561 ctcgttctcg tagcagaact tgtacagctc gtccatgccg ccggtggagt ggcggccctc 
     1621 ggagcgttcg tactgttcca ccacggtgta gtcctcgttg tgggaggtga tgtccaactt 
     1681 gcggtccacg ttgtaggcgc cgggcatctg cacgggcttc ttggccttgt aggtggtctt 
     1741 gaagtccgcc aggtagcgcc cgccgtcctt caggcgcagg gccatcttaa tgtcgccctt 
     1801 cagcacgccg tcctcggggt acaaccgctc ggtggacgct tcccagccca ttgtcttctt 
     1861 ctgcattacg gggccgtcag gagggaagtt ggtgccgcgg agcttcacct tgtagatcag 
     1921 ggtgccgtcc tccagggagg tgtcctgggt cacggtcacg gcgccgccgt cctcgaagtt 
     1981 catcacgcgg tcccacttga agccctcggg gaaggactgc ttatagtagt cggggatgtc 
     2041 ggcggggtgc ttggtgaagg ccctggagcc gtacatgaac tgaggggaca ggatgtccca 
     2101 ggagaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta 
     2161 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat 
     2221 gtgcaccttg aaccgcatga actccttgat cactgcctcg cccttgctca ccacgttgat 
     2281 gtcgatctgg tccaggggga tgtactcgcc ctcgctggtg atcctgctct tggcggccgc 
     2341 agacagtctc cgcaggcgct cgccattgct gctcagcctc atatccagct ccagcttctg 
     2401 gaactcatcc tcgtcgtgtc ccagctgctc cagcagccga aacagctcct catcataggt 
     2461 ctttctgaac cgggccaggg caaagccgta acaggacttg gccacgatgt cgcacacgca 
     2521 gtgggccacc tctgtccagt tatcgtcgat ccagccgaag cctctagggc agatggccca 
     2581 caccagggga aacagaatcc acagcatgga gaaggtaaac acggcgaact tcagcacgtg 
     2641 caggctgtgc tcggcgcctg tatcctgggc cagctgggcc agccggctat actgcttggc 
     2701 cacgatgctc atcacgatag agtaggcgaa gataaaccag aaacagctaa agccatacca 
     2761 ggcgtaggcg ccattcctca gcctagggtc gccctctgcg tagaactcgg ccagcacgcc 
     2821 agacatcagc atggcaaaga tgatggcgct acaagacacg cgataggggg ccctcagctg 
     2881 atacagcaga tcgtacacca gcattgggca ggtcagcatg tagtcggcat acctgtaatt 
     2941 gccgaagccg atgatgaagg ggcccacatc gccgaagttg tggcccagtg tgtcggccac 
     3001 gatcttgccg aagtagctca gcatggcaaa gaaattcaca aacagggcca ggttctgctc 
     3061 ctcctgatag ccggcgtaaa aggaggttct cttgctaggg ccccgagatg tccaggactc 
     3121 atagcccagg tagaatgtgg tgattgtcag cacgataaag caagaccagt gggcgccgat 
     3181 ggccacggag ttcagcttgg tatccagata ccagccgtcc attgtgccca cgtagaacat 
     3241 ctggtcggtg cctggtgcgt gtgccatggt ggctagcata acttcgtata aagtatccta 
     3301 tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag 
     3361 aataacttcg tataatgtat gctatacgaa gttatgaatt cgatatcaag cttatcgata 
     3421 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     3481 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     3541 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     3601 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     3661 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     3721 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     3781 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 
     3841 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     3901 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     3961 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag 
     4021 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc 
     4081 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat 
     4141 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa 
     4201 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag 
     4261 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct 
     4321 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt 
     4381 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc 
     4441 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc 
     4501 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa 
     4561 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 
     4621 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 
     4681 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 
     4741 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg 
     4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     4981 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     5101 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 
     5221 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 
     5281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 
     5341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 
     5401 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 
     5461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     6301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     6781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     7261 tcctggcctt ttgctggcct tttgctcaca tgt 
//
