LOCUS       pAAV-Ef1a-DIO\rsChRmine-oScarlett-Kv2.1-WPRE        7488 bp    DNA     circular      8-FEB-2022
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14255283|
COMMENT     VNTDBDATE|-14255283|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-Ef1a-DIO rsChRmine-oScarlett-Kv2.1-WPRE|
COMMENT     VNTAUTHORNAME|Charu|
COMMENT     VNTAUTHOREML|charur@stanford.edu|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-Ef1a-DIO rsChRmine-oScarlett-Kv2.1-WPRE" 0 7488 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" 
COMMENT         "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaII" "AvrII" "BaeI" "BanI" "BanII" "BbeI" "Bbr7I" "BbsI" "BbvCI" 
COMMENT         "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" "BplI" "BpmI" 
COMMENT         "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" "BsaWI" "BsaXI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" 
COMMENT         "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" "Bsp24I" "BspCNI" 
COMMENT         "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" "BsrGI" "BsrI" "BssHII" 
COMMENT         "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" 
COMMENT         "ChaI" "CjeI" "CjePI" "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" 
COMMENT         "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" 
COMMENT         "EcoNI" "EcoO109I" "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" 
COMMENT         "HaeIV" "HgaI" "HhaI" "Hin4I" "HincII" "HinfI" "HinP1I" "HpaI" "HpaII" 
COMMENT         "HphI" "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4III" "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" 
COMMENT         "MaeIII" "MboI" "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" 
COMMENT         "MseI" "MslI" "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NdeI" "NgoMIV" 
COMMENT         "NheI" "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" 
COMMENT         "PciI" "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" 
COMMENT         "PshAI" "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" "Sse232I" 
COMMENT         "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" "StyI" 
COMMENT         "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" "Tsp45I" 
COMMENT         "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" "UnbI" "UthSI" 
COMMENT         "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7488 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 5 2417 0 3825 0 5427 0 
COMMENT            5924 0 7170 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 15 41 0 52 0 288 0 390 0 
COMMENT            575 0 1090 0 1207 0 2009 0 2201 0 3298 0 4222 0 4527 0 4837 0 4848 0 
COMMENT            5304 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1421 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 3611 0 4206 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3592 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 157 0 3604 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3460 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 7 0 558 0 1063 0 4890 0 0 
COMMENT            "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 4839 0 4850 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 4837 0 4848 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4069 0 5677 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AceIII" "cagctc" 14 18 8 1724 0 1793 0 2594 0 
COMMENT             2651 0 3892 0 5353 1 5550 1 6292 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AciI" "ccgc" 2 4 91 38 0 143 1 147 0 343 1 
COMMENT             380 0 460 0 494 0 496 1 676 0 679 0 800 1 840 0 842 1 846 1 889 1 
COMMENT             904 1 971 0 986 0 995 1 1031 1 1043 0 1084 1 1099 1 1103 1 1643 0 
COMMENT             1671 1 1735 1 1794 0 1808 1 1877 1 1942 0 1956 0 2021 0 2091 0 2093 
COMMENT             1 2160 0 2183 1 2238 1 2337 1 2360 1 2429 0 2529 1 2533 0 2546 0 
COMMENT             3925 1 3938 0 3951 0 4061 1 4102 1 4116 0 4118 1 4137 1 4147 0 4193 
COMMENT             0 4201 0 4429 1 4489 0 4735 1 4744 1 4748 0 4853 1 4903 1 4929 1 
COMMENT             4942 0 4979 1 4993 1 4996 1 5024 0 5051 0 5456 0 5482 0 5492 0 5531 
COMMENT             0 5705 1 5752 0 5851 1 5960 1 6037 1 6081 0 6202 1 6248 0 6439 1 
COMMENT             6530 1 6892 0 6901 1 7036 0 7146 1 7267 1 7286 1 7413 1 7441 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 436 0 5410 0 5994 0 6367 
COMMENT             0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 8 1311 0 1576 1 1949 1 
COMMENT             1979 1 2312 1 2810 1 5930 0 6942 1 0 "") 
COMMENT        #18=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 4217 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 621 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflIII" "acrygt" 2 0 3 151 0 875 0 7484 0 0 
COMMENT             "") #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 321 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 5 71 0 2288 0 4349 0 
COMMENT             4823 0 6596 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 2 1839 0 4293 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 2 4711 0 5236 1 0 
COMMENT             "") 
COMMENT        #25=(CRSite (CStringList) "AluI" "agct" 3 0 41 12 0 159 0 205 0 580 0 
COMMENT             938 0 1068 0 1212 0 1304 0 1472 0 1483 0 1613 0 1622 0 1714 0 1783 
COMMENT             0 2098 0 2332 0 2584 0 2590 0 2620 0 2641 0 2869 0 2942 0 3073 0 
COMMENT             3212 0 3391 0 3606 0 3882 0 4450 0 4567 0 4881 0 5110 0 5367 0 5545 
COMMENT             0 5564 0 6243 0 6306 0 6406 0 6927 0 7184 0 7230 0 7320 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AlwI" "ggatc" 10 11 15 566 1 1360 0 1416 1 
COMMENT             1429 0 2510 1 2738 1 4186 0 5957 0 5961 1 6278 0 6741 1 6742 0 6838 
COMMENT             1 6840 0 6926 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 2593 0 3250 0 7075 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 2 1023 0 2843 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ApaI" "gggccc" 6 0 4 859 0 3068 0 3160 0 3298 
COMMENT             0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ApoI" "raatty" 2 0 7 745 0 1331 0 3228 0 3592 
COMMENT             0 3635 0 5380 0 5391 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1546 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "AseI" "attaat" 3 0 1 6421 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 8 211 0 1879 0 2185 0 2485 
COMMENT             0 4104 0 4737 0 6232 0 6454 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 2978 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 19 249 0 324 0 696 0 955 0 
COMMENT             1015 0 1180 0 1191 0 1892 0 2087 0 2117 0 2156 0 2345 0 2849 0 2963 
COMMENT             0 3368 0 3443 0 4893 0 5156 0 6643 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BanII" "grgcyc" 6 0 9 631 0 859 0 1070 0 2410 
COMMENT             0 3068 0 3160 0 3298 0 3969 0 5126 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 7 1184 0 1896 0 2160 0 
COMMENT             2853 0 2967 0 3372 0 4897 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 3 2037 1 4139 1 4421 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 2042 1 4144 1 4416 0 0 
COMMENT             "") 
COMMENT        #42=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 4 1144 0 2973 0 3069 0 
COMMENT             4519 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BbvI" "gcagc" 14 18 27 21 0 214 0 540 1 703 1 
COMMENT             748 1 1045 1 1470 1 1687 1 2551 1 2607 1 2641 0 3684 1 3940 1 4016 
COMMENT             1 4112 1 4205 1 4868 1 4890 0 5027 0 5551 1 6162 1 6553 0 6856 1 
COMMENT             7062 1 7065 1 7155 0 7487 1 0 "") 
COMMENT        #44=(CRSite (CStringList) "BccI" "ccatc" 10 11 16 133 0 163 1 1032 1 
COMMENT             1252 1 1749 0 1754 1 1986 0 2762 1 3032 1 3368 1 4759 1 5182 1 5214 
COMMENT             0 6139 1 6426 1 6550 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BceAI" "acggc" 18 20 19 414 1 465 1 958 1 
COMMENT             1133 1 1168 1 1943 1 1988 1 2054 1 2105 1 2147 1 2170 0 2249 1 2381 
COMMENT             1 2666 1 2833 0 3395 1 3938 0 5170 0 6984 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BcefI" "acggc" 18 19 19 415 1 466 1 959 1 
COMMENT             1134 1 1169 1 1944 1 1989 1 2055 1 2106 1 2148 1 2170 0 2250 1 2382 
COMMENT             1 2667 1 2833 0 3396 1 3938 0 5170 0 6985 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 4530 0 6058 1 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 7 1434 1 2867 0 3407 0 
COMMENT             3500 0 3685 1 5759 0 7286 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 2443 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BfaI" "ctag" 2 0 14 131 0 218 0 329 0 734 0 
COMMENT             2760 0 2979 0 3291 0 3469 0 4346 0 4760 0 5044 0 6403 0 6738 0 6991 
COMMENT             0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 4552 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 9 43 0 1013 0 1189 0 
COMMENT             1306 0 2762 0 2871 0 3465 0 4853 0 6478 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BglII" "agatct" 2 0 2 808 0 4228 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 3 2568 0 3213 0 4563 0 0 
COMMENT             "") 
COMMENT        #55=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 14 254 0 859 0 2350 0 
COMMENT             2421 0 3068 0 3160 0 3298 0 3424 0 3829 0 3982 0 4300 0 5431 0 5928 
COMMENT             0 7174 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BmrI" "actggg" 12 11 4 362 0 3175 1 4254 1 
COMMENT             6556 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 3472 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 2 1746 0 4237 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BpmI" "ctggag" 23 21 9 1694 1 1741 0 2111 1 
COMMENT             2280 0 2570 1 2609 1 4276 1 4472 0 6527 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 4 1144 0 2973 0 3069 0 
COMMENT             4519 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 7 666 0 918 1 1352 1 
COMMENT             5992 0 6860 0 7101 1 7399 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 562 0 2833 0 3427 0 
COMMENT             4732 0 5197 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2478 0 2904 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 13 57 0 1181 0 1656 0 
COMMENT             1893 0 2157 0 2850 0 2964 0 3369 0 4066 0 4834 0 4894 0 5674 0 6056 
COMMENT             0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 4625 0 6530 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 41 41 0 52 0 391 0 494 0 
COMMENT             662 0 840 0 989 0 990 0 1167 0 1326 0 1603 0 1684 0 1747 0 1813 0 
COMMENT             1837 0 2008 0 2091 0 2128 0 2140 0 2200 0 2257 0 2293 0 2317 0 2488 
COMMENT             0 2671 0 2779 0 2860 0 2978 0 3321 0 3379 0 3460 0 3920 0 3992 0 
COMMENT             4024 0 4116 0 4276 0 4495 0 4653 0 4837 0 4848 0 7324 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 5 246 0 321 0 6300 0 7131 
COMMENT             0 7278 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 4 1809 1 1851 1 
COMMENT             4709 0 5236 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BscAI" "gcatc" 10 12 13 1908 0 2893 1 4211 0 
COMMENT             4249 0 4338 0 4893 1 4929 0 5445 1 5535 0 5898 1 6143 0 6338 1 7390 
COMMENT             1 0 "") 
COMMENT        #71=(CRSite (CStringList) "BseMII" "ctcag" 16 14 21 23 1 98 0 1158 0 
COMMENT             1266 1 1388 0 2267 1 2582 0 2987 0 3083 0 3227 0 4180 0 4533 0 4577 
COMMENT             0 4650 0 4797 1 4872 0 5448 0 6109 0 6626 1 6792 1 7201 1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BseRI" "gaggag" 17 15 5 1706 1 2633 1 3245 1 
COMMENT             3741 1 3802 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BseYI" "cccagc" 2 6 9 669 1 867 0 1691 1 1765 
COMMENT             1 2038 0 2616 0 2869 1 4670 1 7180 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 439 0 797 1 1889 1 
COMMENT             2855 0 4477 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 147 0 1013 0 2533 0 
COMMENT             4748 0 6078 0 6227 0 7150 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 8 1070 0 2421 0 2847 0 
COMMENT             3829 0 5431 0 5928 0 6013 0 7174 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 50 59 0 131 0 259 0 
COMMENT             260 0 334 0 396 0 397 0 512 0 582 0 663 0 917 0 990 0 991 0 1049 0 
COMMENT             1061 0 1122 0 1855 0 1876 0 2010 0 2027 0 2076 0 2300 0 2355 0 2356 
COMMENT             0 2525 0 2593 0 2672 0 2779 0 2780 0 2801 0 2960 0 3100 0 3806 0 
COMMENT             3857 0 3956 0 4025 0 4117 0 4128 0 4277 0 4495 0 4765 0 4837 0 4978 
COMMENT             0 5304 0 5305 0 5558 0 7006 0 7285 0 7451 0 7469 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 12 716 0 735 0 936 0 1280 
COMMENT             1 2547 0 3782 0 4583 1 4625 0 5559 0 5601 1 5754 1 6530 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 4583 1 5559 0 5601 1 0 
COMMENT             "") 
COMMENT        #81=(CRSite (CStringList) "BsmFI" "gggac" 16 20 10 270 1 850 1 1782 0 
COMMENT             2171 1 2276 1 2295 0 2599 1 3906 0 4066 1 4077 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 21 254 0 631 0 859 0 
COMMENT             1070 0 2350 0 2410 0 2421 0 2847 0 3068 0 3160 0 3298 0 3424 0 3829 
COMMENT             0 3969 0 3982 0 4300 0 5126 0 5431 0 5928 0 6013 0 7174 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 6 2305 1 2724 1 
COMMENT             3861 1 5696 0 6790 1 6968 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 21 24 1 97 0 1157 0 
COMMENT             1267 1 1387 0 2268 1 2581 0 2986 0 3082 0 3226 0 4179 0 4532 0 4576 
COMMENT             0 4649 0 4798 1 4871 0 5447 0 6108 0 6627 1 6793 1 7202 1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 5651 0 5756 0 6764 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 766 0 3095 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 6 1031 1 1099 1 2023 0 
COMMENT             4744 1 5053 0 5754 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 2559 1 3863 1 6362 0 
COMMENT             6536 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 8 321 0 942 0 1796 0 2878 
COMMENT             0 3266 0 4128 0 5092 0 6511 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1777 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BsrI" "actgg" 7 5 21 357 0 378 0 554 0 823 0 
COMMENT             1019 1 2732 1 3181 1 3362 1 3657 0 3858 0 4260 1 4293 1 5286 0 5951 
COMMENT             0 6121 1 6390 0 6433 0 6551 0 6957 0 7069 1 7082 1 0 "") 
COMMENT        #94=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1546 0 4789 
COMMENT             0 4874 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 639 1 2390 1 5620 1 
COMMENT             5927 0 7311 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 2 1022 0 2842 0 0 
COMMENT             "") #97=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 2 2321 0 4724 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 10 1765 0 2236 0 2293 0 
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COMMENT                               (CParagraph 117699064 (8 0) 1 1 1 0 178) 
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COMMENT                             "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
COMMENT                       #432=(CRSFolderPar 
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COMMENT                               (CParagraph 117699128 (8 0) 1 1 1 0 178) 
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COMMENT                       #433=(CRSFolderPar 
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COMMENT                               (CParagraph 117700792 (8 0) 1 1 1 0 178) 
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COMMENT                             "GCAGCNNNNNNNNNNNNN" "CGTCGNNNNNNNNNNNNN") 
COMMENT                       #434=(CRSFolderPar 
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COMMENT                               (CParagraph 117700856 (8 0) 1 1 1 0 178) 
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COMMENT                             "CCATCNNNNNN" "GGTAGNNNNNN") 
COMMENT                       #435=(CRSFolderPar 
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COMMENT                               (CParagraph 117700216 (8 0) 1 1 1 0 178) 
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COMMENT                             "ACGGCNNNNNNNNNNNNNNN" "TGCCGNNNNNNNNNNNNNNN") 
COMMENT                       #436=(CRSFolderPar 
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COMMENT                               (CParagraph 117700952 (8 0) 1 1 1 0 178) 
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COMMENT                             "ACGGCNNNNNNNNNNNNNN" "TGCCGNNNNNNNNNNNNNN") 
COMMENT                       #437=(CRSFolderPar 
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COMMENT                               (CParagraph 117699960 (8 0) 1 1 1 0 178) 
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COMMENT                             "CGANNNNNNTGCNNNNNNNNNNNNN" 
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COMMENT                       #438=(CRSFolderPar 
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COMMENT                             "GTATCCNNNNNNN" "CATAGGNNNNNNN") 
COMMENT                       #439=(CRSFolderPar 
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COMMENT                               (CParagraph 117700312 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                              "BclI: 1 site") 1 5 "TGATCA" "ACTAGT") 
COMMENT                       #442=(CRSFolderPar 
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COMMENT                       #443=(CRSFolderPar 
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COMMENT                               (CParagraph 117700056 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                              "BfrBI: 1 site") 3 3 "ATGCAT" "TACGTA") 
COMMENT                       #446=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700088 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BglI: 9 sites") 7 4 "GCCNNNNNGGC" 
COMMENT                             "CGGNNNNNCCG") 
COMMENT                       #447=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700664 (8 0) 1 1 1 0 178) 
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COMMENT                             "TCTAGA") 
COMMENT                       #448=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700248 (8 0) 1 1 1 0 178) 
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COMMENT                             "CGANTCG") 
COMMENT                       #449=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 116838152 (8 0) 1 1 1 0 178) 
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COMMENT                             "CMCGKG") 
COMMENT                       #450=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 116838752 (8 0) 1 1 1 0 178) 
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COMMENT                       #451=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700344 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BmrI: 4 sites") 11 10 
COMMENT                             "ACTGGGNNNNNN" "TGACCCNNNNNN") 
COMMENT                       #452=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700408 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #453=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #58# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                              "BmtI: 1 site") 5 1 "GCTAGC" "CGATCG") 
COMMENT                       #455=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700440 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BplI: 2 sites") 38 1 
COMMENT                             "NNNNNNNNNNNNNNGAGNNNNNCTCNNNNNNNNNNNNNN" 
COMMENT                             "NNNNNNNNNNNNNNCTCNNNNNGAGNNNNNNNNNNNNNN") 
COMMENT                       #456=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700536 (8 0) 1 1 1 0 178) 
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COMMENT                             "CTGGAGNNNNNNNNNNNNNNNNN" "GACCTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #457=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700472 (8 0) 1 1 1 0 178) 
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COMMENT                             "GGANTCG") 
COMMENT                       #458=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700568 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BpuEI: 7 sites") 22 20 
COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #459=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700600 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaAI: 5 sites") 3 3 "YACGTR" 
COMMENT                             "RTGCAY") 
COMMENT                       #460=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaBI: 2 sites") 5 5 "GATNNNNATC" 
COMMENT                             "CTANNNNTAG") 
COMMENT                       #461=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaHI: 13 sites") 2 4 "GRCGYC" 
COMMENT                             "CYGCRG") 
COMMENT                       #462=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701016 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaI: 2 sites") 7 11 
COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                       #463=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701048 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaJI: 41 sites") 1 5 "CCNNGG" 
COMMENT                             "GGNNCC") 
COMMENT                       #464=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701368 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaWI: 5 sites") 1 5 "WCCGGW" 
COMMENT                             "WGGCCW") 
COMMENT                       #465=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701816 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaXI: 4 sites") 21 18 
COMMENT                             "ACNNNNNCTCCNNNNNNNNNNN" "TGNNNNNGAGGNNNNNNNNNNN") 
COMMENT                       #466=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BscAI: 13 sites") 9 11 
COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
COMMENT                       #467=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseMII: 21 sites") 15 13 
COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
COMMENT                       #468=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701400 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseRI: 5 sites") 16 14 
COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
COMMENT                       #469=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701432 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseYI: 9 sites") 1 5 "CCCAGC" 
COMMENT                             "GGGTCG") 
COMMENT                       #470=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117700984 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsgI: 5 sites") 22 20 
COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #471=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701560 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiEI: 7 sites") 4 2 "CGRYCG" 
COMMENT                             "GCYRGC") 
COMMENT                       #472=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701688 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiHKAI: 8 sites") 5 1 "GWGCWC" 
COMMENT                             "CWCGWG") 
COMMENT                       #473=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiWI: 0 sites") 1 5 "CGTACG" 
COMMENT                             "GCATGC") 
COMMENT                       #474=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701944 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BslI: 50 sites") 7 4 
COMMENT                             "CCNNNNNNNGG" "GGNNNNNNNCC") 
COMMENT                       #475=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701720 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmAI: 12 sites") 6 10 
COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
COMMENT                       #476=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701080 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmBI: 3 sites") 7 11 
COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
COMMENT                       #477=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701784 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmFI: 10 sites") 15 19 
COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
COMMENT                       #478=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701912 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmI: 0 sites") 7 5 "GAATGCNN" 
COMMENT                             "CTTACGNN") 
COMMENT                       #479=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 116838712 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp1286I: 21 sites") 5 1 "GDGCHC" 
COMMENT                             "CHCGDG") 
COMMENT                       #480=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 116838992 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp24I: 6 sites") 24 19 
COMMENT                             "GACNNNNNNTGGNNNNNNNNNNNNN" 
COMMENT                             "CTGNNNNNNACCNNNNNNNNNNNNN") 
COMMENT                       #481=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701336 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspCNI: 21 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
COMMENT                       #482=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701176 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspEI: 0 sites") 1 5 "TCCGGA" 
COMMENT                             "AGGCCT") 
COMMENT                       #483=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspHI: 3 sites") 1 5 "TCATGA" 
COMMENT                             "AGTACT") 
COMMENT                       #484=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701112 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspMI: 2 sites") 10 14 
COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
COMMENT                       #485=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701464 (8 0) 1 1 1 0 178) 
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COMMENT                             "GGCGAG") 
COMMENT                       #486=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701496 (8 0) 1 1 1 0 178) 
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COMMENT                             "CGTTACNNN") 
COMMENT                       #487=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701976 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrFI: 8 sites") 1 5 "RCCGGY" 
COMMENT                             "YGGCCR") 
COMMENT                       #488=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701144 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #489=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #92# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #92# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1777 " 1))))) 
COMMENT                              "BsrGI: 1 site") 1 5 "TGTACA" "ACATGT") 
COMMENT                       #491=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701208 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrI: 21 sites") 6 4 "ACTGGNN" 
COMMENT                             "TGACCNN") 
COMMENT                       #492=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701272 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BssHII: 5 sites") 1 5 "GCGCGC" 
COMMENT                             "CGCGCG") 
COMMENT                       #493=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117701528 (8 0) 1 1 1 0 178) 
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COMMENT                       #494=(CRSFolderPar 
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COMMENT                               (CParagraph 117701592 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstAPI: 2 sites") 7 4 
COMMENT                             "GCANNNNNTGC" "CGTNNNNNACG") 
COMMENT                       #495=(CRSFolderPar 
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COMMENT                               (CParagraph 117701624 (8 0) 1 1 1 0 178) 
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COMMENT                             "AAGCTT") 
COMMENT                       #496=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702744 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstEII: 2 sites") 1 6 "GGTNACC" 
COMMENT                             "CCANTGG") 
COMMENT                       #497=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702680 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstF5I: 10 sites") 7 5 "GGATGNNN" 
COMMENT                             "CCTACNNN") 
COMMENT                       #498=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702328 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstKTI: 28 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #499=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702808 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 31 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #500=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702360 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 32 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #501=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117703000 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstXI: 3 sites") 8 4 
COMMENT                             "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #502=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702712 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstYI: 11 sites") 1 5 "RGATCY" 
COMMENT                             "YCTAGR") 
COMMENT                       #503=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702296 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstZ17I: 0 sites") 3 3 "GTATAC" 
COMMENT                             "CATATG") 
COMMENT                       #504=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702392 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsu36I: 0 sites") 2 5 "CCTNAGG" 
COMMENT                             "GGANTCC") 
COMMENT                       #505=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtgI: 12 sites") 1 5 "CCRYGG" 
COMMENT                             "GGYRCC") 
COMMENT                       #506=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702872 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 62 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #507=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702840 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtsI: 7 sites") 8 6 "GCAGTGNNN" 
COMMENT                             "CGTCACNNN") 
COMMENT                       #508=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702008 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 60 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #509=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702488 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CdiI: 14 sites") 4 4 "CATCG" 
COMMENT                             "GTAGC") 
COMMENT                       #510=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702904 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ChaI: 28 sites") 5 1 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #511=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702776 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 22 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #512=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjePI: 20 sites") 26 20 
COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNNAGNNNNNNNNNNNNNNN") 
COMMENT                       #513=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117698072 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ClaI: 2 sites") 2 4 "ATCGAT" 
COMMENT                             "TAGCTA") 
COMMENT                       #514=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702040 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Csp6I: 13 sites") 1 3 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #515=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702968 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviAII: 27 sites") 1 3 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #516=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702552 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviJI: 175 sites") 2 2 "RGCY" 
COMMENT                             "YCGR") 
COMMENT                       #517=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 117702584 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DdeI: 22 sites") 1 4 "CTNAG" 
COMMENT                             "GANTC") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     polyA_signal    4233..4711
                     /vntifkey="25"
                     /label=hGH\polyA
     misc_feature    4751..4891
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      4983..5289
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    5808..6665
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6816..7483
                     /vntifkey="33"
                     /label=pUC\ori
     promoter        156..1419
                     /vntifkey="29"
                     /label=EF-1a
     misc_feature    3605..4213
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    1426..1459
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    complement(3473..3506)
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    1510..1543
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(3557..3590)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(2467..2526)
                     /vntifkey="21"
                     /label=TS
     misc_feature    complement(1579..1773)
                     /vntifkey="21"
                     /label=Kv2.1
     enhancer        complement(1558..1578)
                     /vntifkey="9"
                     /label=ER\export
     misc_feature    complement(1774..2466)
                     /vntifkey="21"
                     /label=mScarlet
     misc_feature    2185..2187
                     /vntifkey="21"
                     /label=E95D
     misc_feature    complement(3025..3027)
                     /vntifkey="21"
                     /label=I146M
     misc_feature    complement(2941..2943)
                     /vntifkey="21"
                     /label=G174S
     misc_feature    complement(2536..3462)
                     /vntifkey="21"
                     /label=ChRmine
BASE COUNT     1529 a      2023 c      2084 g      1852 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg 
     1441 atactttata cgaagttatg cagaatggta gctggattgt agctgctatt agcaatatga 
     1501 aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc cctattacac 
     1561 ctcgttctcg tagcagaaga atctggtggc ttcagggaag tccgtggcac agctgatgaa 
     1621 gctgtcaatg ctggacatgc tccgcatgtc gatgacgccc tccgtgcgtg cgggcagagg 
     1681 ggccacgggg ctgggcatgc tgctcatctc cagctcctct ggaggcttgc tctgcggggc 
     1741 catctccttg gtgttgagga tgggctggga ctgcttgtac agctcgtcca tgccgccggt 
     1801 ggagtggcgg ccctcggagc gttcgtactg ttccaccacg gtgtagtcct cgttgtggga 
     1861 ggtgatgtcc aacttgcggt ccacgttgta ggcgccgggc atctgcacgg gcttcttggc 
     1921 cttgtaggtg gtcttgaagt ccgccaggta gcgcccgccg tccttcaggc gcagggccat 
     1981 cttaatgtcg cccttcagca cgccgtcctc ggggtacaac cgctcggtgg acgcttccca 
     2041 gcccattgtc ttcttctgca ttacggggcc gtcaggaggg aagttggtgc cgcggagctt 
     2101 caccttgtag atcagggtgc cgtcctccag ggaggtgtcc tgggtcacgg tcacggcgcc 
     2161 gccgtcctcg aagttcatca cgcggtccca cttgaagccc tcggggaagg actgcttata 
     2221 gtagtcgggg atgtcggcgg ggtgcttggt gaaggccctg gagccgtaca tgaactgagg 
     2281 ggacaggatg tcccaggaga agggcagggg gccacccttg gtcaccttca gcttggcggt 
     2341 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt 
     2401 catggagccc tccatgtgca ccttgaaccg catgaactcc ttgatcactg cctcgccctt 
     2461 gctcaccacg ttgatgtcga tctggtccag ggggatgtac tcgccctcgc tggtgatcct 
     2521 gctcttggcg gccgcagaca gtctccgcag gcgctcgcca ttgctgctca gcctcatatc 
     2581 cagctccagc ttctggaact catcctcgtc gtgtcccagc tgctccagca gccgaaacag 
     2641 ctcctcatca taggtctttc tgaaccgggc cagggcaaag ccgtaacagg acttggccac 
     2701 gatgtcgcac acgcagtggg ccacctctgt ccagttatcg tcgatccagc cgaagcctct 
     2761 agggcagatg gcccacacca ggggaaacag aatccacagc atggagaagg taaacacggc 
     2821 gaacttcagc acgtgcaggc tgtgctcggc gcctgtatcc tgggccagct gggccagccg 
     2881 gctatactgc ttggccacga tgctcatcac gatagagtag gcgaagataa accagaaaca 
     2941 gctaaagcca taccaggcgt aggcgccatt cctcagccta gggtcgccct ctgcgtagaa 
     3001 ctcggccagc acgccagaca tcagcatggc aaagatgatg gcgctacaag acacgcgata 
     3061 gggggccctc agctgataca gcagatcgta caccagcatt gggcaggtca gcatgtagtc 
     3121 ggcatacctg taattgccga agccgatgat gaaggggccc acatcgccga agttgtggcc 
     3181 cagtgtgtcg gccacgatct tgccgaagta gctcagcatg gcaaagaaat tcacaaacag 
     3241 ggccaggttc tgctcctcct gatagccggc gtaaaaggag gttctcttgc tagggccccg 
     3301 agatgtccag gactcatagc ccaggtagaa tgtggtgatt gtcagcacga taaagcaaga 
     3361 ccagtgggcg ccgatggcca cggagttcag cttggtatcc agataccagc cgtccattgt 
     3421 gcccacgtag aacatctggt cggtgcctgg tgcgtgtgcc atggtggcta gcataacttc 
     3481 gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt 
     3541 tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat gaattcgata 
     3601 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     3661 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     3721 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     3781 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     3841 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     3901 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     3961 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 
     4021 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     4081 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     4141 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     4201 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc 
     4261 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa 
     4321 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg 
     4381 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg 
     4441 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt 
     4501 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag 
     4561 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt 
     4621 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca 
     4681 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc 
     4741 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 
     4801 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 
     4861 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg 
     4921 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 
     4981 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     5041 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     5101 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     5161 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 
     5221 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     5281 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga 
     5341 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     5401 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 
     5461 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 
     5521 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 
     5581 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 
     5641 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 
     5701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
     5761 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 
     5821 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
     5881 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
     5941 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
     6001 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
     6061 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
     6121 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
     6181 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
     6241 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
     6301 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
     6361 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
     6421 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
     6481 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
     6541 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
     6601 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
     6661 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
     6721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 
     6781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     6841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     6901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     6961 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     7021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     7081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     7141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     7201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     7261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     7321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     7381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     7441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt 
//
