LOCUS       Exported                7072 bp DNA     circular SYN 15-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Matthew Millette
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1761..1802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..58
                     /label=Primer 4
     misc_feature    36..56
                     /label=overhang
     primer_bind     36..55
                     /label=pCW F
     promoter        56..370
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    64..82
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    171..189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    207..225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    278..296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    353..377
                     /label=overhang
     primer_bind     complement(353..377)
                     /label=tet linearize rev
     primer_bind     complement(358..416)
                     /label=Tet-Lin MCS R
     misc_feature    384..415
                     /label=7
     primer_bind     408..439
                     /label=42f
     primer_bind     409..435
                     /label=Primer 3a`f
     primer_bind     409..435
                     /label=Primer 3`g
     primer_bind     409..435
                     /label=Primer 3a`e
     primer_bind     409..435
                     /label=Primer 3`f
     primer_bind     409..435
                     /label=Primer 3a`d
     primer_bind     409..435
                     /label=Primer 3`e
     primer_bind     409..435
                     /label=Primer 3a`c
     primer_bind     409..435
                     /label=Primer 3`d
     primer_bind     409..435
                     /label=Primer 3a`b
     primer_bind     409..435
                     /label=Primer 3`c
     primer_bind     409..435
                     /label=Primer 3aa
     primer_bind     409..435
                     /label=Primer 3`b
     primer_bind     409..435
                     /label=Primer 3a
     misc_feature    416..420
                     /label=Kozak Sequence
     misc_feature    416
                     /label=NotI
     CDS             421..423
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="M"
     CDS             424..1137
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(780..797)
                     /label=64
     primer_bind     1016..1035
                     /label=134
     primer_bind     1081..1099
                     /label=81
     primer_bind     complement(1118..1140)
                     /label=43r
     primer_bind     complement(1145..1165)
                     /label=156
     primer_bind     1149..1168
                     /label=IRES cODC OE  F
     misc_feature    1171..1760
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)
                     Includes M-Loop and Preferred, rather than Minimal AUG.
                     
                     Originally from Addgene plasmid - pMIGR AMPK KD (#27296)
                     
                     
                     "
     primer_bind     complement(1734..1753)
                     /label=IRES 670 OE R
     primer_bind     complement(1740..1771)
                     /label=30R
     primer_bind     1746..1836
                     /label=31U
     primer_bind     1748..1777
                     /label=bridge gap -- single stranded
     CDS             1761..1802
                     /codon_start=1
                     /label=G protein subunit alpha i2 (GNAI2)
                     /translation="GCTVSAEDKAAAER"
     CDS             1803..1817
                     /codon_start=1
                     /label=linker
                     /translation="GSGSG"
     primer_bind     1810..1842
                     /label=32F
     CDS             1824..2516
                     /codon_start=1
                     /product="codon optimized (rat)
                     Different enough that mt2 can't be aligned to it
                     "
                     /label=scarlet-I
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1942..1961)
                     /label=136
     primer_bind     2497..2555
                     /label=Primer 2
     primer_bind     complement(2497..2529)
                     /label=33R
     primer_bind     2501..2555
                     /label=FIXIT
     primer_bind     2502..2542
                     /label=34F
     misc_feature    2523..2528
                     /label=SphI
     misc_feature    2529..2638
                     /label=miRE 5'
     primer_bind     complement(2530..2550)
                     /label=ultramer amp R
     primer_bind     2624..2656
                     /label=original 97mer F
     primer_bind     2624..2656
                     /label=97mer forward
     primer_bind     2629..2656
                     /label=RF miRE Forward
     misc_feature    2639..2735
                     /label=97mer
     misc_feature    2656..2657
                     /label=mismatches
     misc_feature    2658..2678
                     /label=guide
     ncRNA           2681..2695
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    2698..2719
                     /label=MIMAT0000039 target
     primer_bind     complement(2721..2757)
                     /label=original 97mer R
     primer_bind     complement(2721..2757)
                     
     primer_bind     complement(2721..2743)
                     /label=RF miRE Rev
     misc_feature    2736..2852
                     
     misc_feature    2822..2827
                     /label=polyA signal
     primer_bind     2830..2889
                     /label=Primer 3
     misc_feature    2837..2842
                     /label=polyA signal
     primer_bind     complement(2853..2864)
                     /label=miRE reverse
     primer_bind     complement(2853..2864)
                     /label=miRE reverse N1 anneal
     misc_feature    2865..2889
                     /label=overlap
     misc_feature    2865..2869
                     /label=sphI remnant
     primer_bind     2870..2889
                     /label=HPGK rework blunt F
                     /note="for inserting mIRE cassettenew primer avoids 
                     creating NsiI site"
     promoter        2873..3383
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2976..2997)
                     /label=HPGK reverse seq
     primer_bind     complement(3369..3402)
                     /label=puro del R
     primer_bind     3383..3413
                     /label=puro del F
                     /note="5' 12 = complementary overhang"
     CDS             3393..4139
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4140..4166)
                     /label=pCW R
                     /note="5' 20 = pCAX complement3' 20 = pCW complement"
     misc_feature    complement(4170..4187)
                     /label=seq primer R
     polyA_site      4187..4242
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(4601..4617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4625..4641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4649..4679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4694..4715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4774..4969
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4820..4955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4975..5109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5348..5936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6107..6967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6954..6974
                     /label=Primer 1
     promoter        complement(6968..7072)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctggtcg tcgactggcc gcgaatcgat atgtcgagtt
       61 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      121 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      181 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      241 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      301 atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
      361 tcagatcgcc tggagaaacc ggtcggccgc gcacgctata ctgcgtgcgc ggccgccacc
      421 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      481 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
      541 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      601 ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag
      661 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      721 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      781 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      841 aagctggagt acaacttcaa cagccactac gtctatatca cggccgacaa gcagaagaac
      901 tgcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcgt gcagctcgcc
      961 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1021 tacctgagcc atcagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1081 ctgctggagt tcgtgaccgc cgccgggatt acacatggca tggacgagct gtacaagtag
     1141 cctgcagggt cacctaaata attccgcccc cccctctccc tccccccccc ctaacgttac
     1201 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     1261 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     1321 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     1381 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     1441 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     1501 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     1561 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     1621 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     1681 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     1741 ataatatggc cacaaccatg ggctgcaccg tgagcgccga ggacaaggcg gcggccgagc
     1801 gcggctctgg ttccggcact agtgtgagta aaggagaggc cgttataaaa gagttcatgc
     1861 ggttcaaggt acacatggag ggatcaatga acggacacga gttcgaaatc gaaggggaag
     1921 gagagggcag gccctatgag ggtactcaga ccgccaaact caaagtgaca aagggaggcc
     1981 ctttgccttt ctcttgggac atcttgtcac cacaatttat gtatggctcc cgagcattta
     2041 taaaacaccc agcagacata cccgattact acaagcagtc tttcccagaa ggttttaaat
     2101 gggaaagggt catgaacttt gaggatggtg gtgctgtgac ggtgacgcag gatacttctt
     2161 tggaggacgg aacactcatc tacaaggtaa agctcagggg cacgaacttc ccgccggacg
     2221 gaccagtcat gcagaaaaag actatgggat gggaggcttc caccgaacgg ttgtatcccg
     2281 aggacggtgt tttgaaggga gatataaaaa tggctcttcg gctgaaagat ggaggcagat
     2341 acttggcaga ttttaagacc acgtataagg caaaaaaacc agtgcaaatg ccgggagcat
     2401 ataacgttga ccgcaaactg gacatcacct cacataacga ggattataca gtagtagaac
     2461 agtacgaaag aagcgaaggg aggcacagca ccgggggtat ggatgagctg tataagtgat
     2521 aggcatgctg tttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga
     2581 aacacttgct gggattactt cgacttctta acccaacaga aggctcgaga aggtatattg
     2641 ctgttgacag tgagcgcact ctttctagga ggttgtgata gtgaagccac agatgtatca
     2701 caacctccta gaaagagtat gcctactgcc tcggacttca aggggctaga attcgagcaa
     2761 ttatcttgtt tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg
     2821 aattaaaatg gtataaatta aatcactttt ttcaattgac gcgtcatgct ccggggttgg
     2881 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2941 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     3001 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     3061 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     3121 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     3181 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     3241 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     3301 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     3361 gaatcaccga cctctctccc cagcaattca ccatgtctag actggacaag agcaaagtca
     3421 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     3481 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     3541 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     3601 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     3661 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     3721 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     3781 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     3841 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     3901 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     3961 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     4021 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     4081 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     4141 taagtaagga tcgatccaag gtaccttgag catctgactt ctggctaata aaggaaattt
     4201 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4261 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4321 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     4381 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4441 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4501 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4561 aagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4741 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     4801 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4861 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4921 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     4981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5101 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5521 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     5581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5761 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6001 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6061 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6241 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cg
//
