LOCUS       pPyPGK_EGFP_TM_I        6240 bp ds-DNA     circular     05-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        1..194
                     /label="PyF101"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     misc_feature    complement(195..282)
                     /label="Py ori"
                     /ApEinfo_revcolor=#646464
                     /ApEinfo_fwdcolor=#646464
     polyA_signal    315..369
                     /label="SV40pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        465..977
                     /label="mPgk1 promoter"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     sig_peptide     1052..1114
                     /label="IgGK Signal"
                     /ApEinfo_revcolor=#960096
                     /ApEinfo_fwdcolor=#960096
     CDS             1115..1831
                     /label="eGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            1115..1831
                     /label="Translation 1115-1831"
     CDS             1895..1957
                     /label="PDGFRB TM domain"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     exon            1895..1957
                     /label="Translation 1895-1957"
     protein_bind    2112..2611
                     /label="IRES"
                     /ApEinfo_revcolor=#be6464
                     /ApEinfo_fwdcolor=#be6464
     CDS             2623..3222
                     /label="Pac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     exon            2623..3222
                     /label="Translation 2623-3222"
     polyA_signal    3309..3531
                     /label="bGHpA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(3576..3596)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(3602..3624)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      3747..4084
                     /label="SVori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    4010..4056
                     /label="SV40 polyA core"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     rep_origin      complement(4309..4991)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(5089..5748)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(5089..5748)
                     /label="Translation 5748-5089"
ORIGIN
        1 AGCTGTTGTC ATAGTAATGA TTGGGTGGAA ACATTCCAGG CCTGGGTGGA GAGGCTTTTT
       61 GCTTCCTCTT GCAAAACCAC ACTGACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC
      121 TCTTGCAAAA CCACACTGCC CTCTGGAGGG CAGTTGCCTA GCAACTAACT AAAAGAGGAT
      181 GTCGCACGGC CAGCTGCGGT CAGTTAGTCA CTTCCTGCTT AACTGACTTG ACATTTTCTA
      241 TTTTAAGAGT CGGGAGGAAA ATTACTGTGT TGGAGGCCCT CCGCCATCTT CTGAAGCTGA
      301 ATCGAATTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
      361 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
      421 ATGTATCTTA TCATGTCTGG ATCTGATATC ATCGTCGACC TCGAAATTCT ACCGGGTAGG
      481 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG
      541 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC
      601 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT
      661 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC
      721 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG
      781 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC
      841 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG
      901 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC
      961 TCCGGGCCTT TCGACCTGCA TCTCATCTAG ATctcgagaa ttctcacgcg tcaagtggag
     1021 caaggcaggt ggacagtgga tcgccgccac catggagacc gacaccctct tgctctgggt
     1081 cctgctgctg tgggttccgg gtagtaccgg agacatggtt tctaagggtg aagaattgtt
     1141 caccggagtt gtgcccatcc tggttgagct ggacggagac gttaacggac acaagttctc
     1201 agtgagcggc gaaggagagg gcgacgctac gtacggtaaa ctgacgttga agttcatctg
     1261 tacaacagga aagctgccag tgccctggcc tacactcgtg accaccctca cctacggagt
     1321 ccagtgcttc tcccggtacc ccgaccacat gaaacagcac gatttcttca agtccgcgat
     1381 gcctgagggc tatgttcagg aacgcaccat attcttcaag gacgacggta actataaaac
     1441 cagggctgaa gttaagttcg agggggacac gttggtaaat agaattgagt tgaaaggaat
     1501 cgatttcaaa gaagacggca atattttggg ccacaagctg gagtacaact acaacagcca
     1561 caacgtgtat attatggctg ataagcagaa gaacggcatc aaggtgaact tcaaaatccg
     1621 acacaatatc gaggatggct ctgtgcaact ggccgaccat taccagcaaa acacgcctat
     1681 cggcgacggt ccggttttgc tgccagataa ccactacctc tccacgcaat cagccctctc
     1741 caaggatcct aacgaaaaaa gagaccatat ggttctgctt gaattcgtga cggctgctgg
     1801 gatcaccctg ggaatggatg agctgtataa gaacgccgtg ggccaggata cgcaggaggt
     1861 gatcgtcgtg ccccacagct tgcccttcaa ggtcgtggtt attagtgcta tcctggcgtt
     1921 ggtggtgctg actattatct cccttatcat acttatcatg ctgtggcaaa aaaaaccccg
     1981 ataagatcct tgacttGCGG CCGCTCGATA AGCTTGATAT CGAATTCCGC CCCccccccc
     2041 TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG
     2101 TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA
     2161 CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG
     2221 CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA
     2281 ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC
     2341 GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT
     2401 GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG
     2461 CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA
     2521 TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
     2581 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA GTACAAGCCC
     2641 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
     2701 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
     2761 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
     2821 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
     2881 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
     2941 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
     3001 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
     3061 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
     3121 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
     3181 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG
     3241 CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG CTCGCTGATC
     3301 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
     3361 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
     3421 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
     3481 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA
     3541 GGCGGAAAGA ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
     3601 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     3661 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     3721 CCAGTCGGGA AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC
     3781 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
     3841 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
     3901 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT
     3961 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
     4021 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG
     4081 TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
     4141 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
     4201 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
     4261 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
     4321 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     4381 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     4441 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     4501 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     4561 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     4621 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     4681 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     4741 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     4801 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     4861 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     4921 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     4981 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     5041 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     5101 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     5161 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     5221 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     5281 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     5341 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     5401 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     5461 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     5521 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     5581 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     5641 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     5701 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     5761 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     5821 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     5881 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     5941 ACTCATACTC TTCCTTTTTC AATGACCGCT TTGGAAAAAC AAAGACTGTA TTTCCTGGAA
     6001 ATTAATGTTT ATTCAATAAA CTGTGTATTC AGCTATATCA CATAGTGGTG AGGCTGAAAT
     6061 GAGGCGGGAA GAGGCGGTTG GGGCTTAATT ATATCAATTT GGGTGGCCCC ACAGCGCCTC
     6121 CAAGGCGCCA GTCCTGTTTT GACAAGTTGC CTCTGGAAGC CTCTACAATG CCTCTCTTCT
     6181 TTTTCTCCAG AGTAAGCGGA GGCCAGGGGC CCCCGGCCTC TGCTTAATAC TAAAAAAAAC
//