LOCUS       pPyPGK-HA-EGFP-Myc-TM-I 6329 bp ds-DNA     circular     05-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        1..194
                     /label="PyF101"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     misc_feature    complement(195..282)
                     /label="Py ori"
                     /ApEinfo_revcolor=#646464
                     /ApEinfo_fwdcolor=#646464
     polyA_signal    315..369
                     /label="SV40pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        465..977
                     /label="mPgk1 promoter"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     CDS             1009..1071
                     /label="IgK signal"
                     /ApEinfo_revcolor=#960096
                     /ApEinfo_fwdcolor=#960096
     exon            1009..1071
                     /label="Translation 1009-1071"
     CDS             1072..1098
                     /label="HA"
                     /ApEinfo_revcolor=#ff64ff
                     /ApEinfo_fwdcolor=#ff64ff
     exon            1072..1098
                     /label="Translation 1072-1098"
     CDS             1177..1893
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            1177..1893
                     /label="Translation 1177-1893"
     CDS             1903..1932
                     /label="Myc"
                     /ApEinfo_revcolor=#ff64ff
                     /ApEinfo_fwdcolor=#ff64ff
     exon            1903..1932
                     /label="Translation 1903-1932"
     CDS             1996..2058
                     /label="PDGFRB TM domain"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     exon            1996..2058
                     /label="Translation 1996-2058"
     protein_bind    2201..2700
                     /label="IRES"
                     /ApEinfo_revcolor=#be6464
                     /ApEinfo_fwdcolor=#be6464
     CDS             2712..3311
                     /label="Pac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     exon            2712..3311
                     /label="Translation 2712-3311"
     polyA_signal    3398..3620
                     /label="bGHpA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(3665..3685)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(3691..3713)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      3836..4173
                     /label="SV40 ori pA"
                     /ApEinfo_revcolor=#646464
                     /ApEinfo_fwdcolor=#646464
     rep_origin      complement(4398..5080)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5178..5837)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(5178..5837)
                     /label="Translation 5837-5178"
ORIGIN
        1 AGCTGTTGTC ATAGTAATGA TTGGGTGGAA ACATTCCAGG CCTGGGTGGA GAGGCTTTTT
       61 GCTTCCTCTT GCAAAACCAC ACTGACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC
      121 TCTTGCAAAA CCACACTGCC CTCTGGAGGG CAGTTGCCTA GCAACTAACT AAAAGAGGAT
      181 GTCGCACGGC CAGCTGCGGT CAGTTAGTCA CTTCCTGCTT AACTGACTTG ACATTTTCTA
      241 TTTTAAGAGT CGGGAGGAAA ATTACTGTGT TGGAGGCCCT CCGCCATCTT CTGAAGCTGA
      301 ATCGAATTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
      361 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
      421 ATGTATCTTA TCATGTCTGG ATCTGATATC ATCGTCGACC TCGAAATTCT ACCGGGTAGG
      481 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG
      541 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC
      601 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT
      661 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC
      721 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG
      781 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC
      841 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG
      901 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC
      961 TCCGGGCCTT TCGACCTGCA TCTCATCTAG ATctcgagtg ccgccaccat ggagacagac
     1021 acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga ctatccatat
     1081 gatgttccag attatgctgg ggcccagccg gccagatctc ccgggatccg cgggtttagt
     1141 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     1201 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1261 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1321 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1381 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1441 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1501 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1561 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1621 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1681 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1741 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1801 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1861 gggatcactc tcggcatgga cgagctgtac aagtccgtcg acgaacaaaa actcatctca
     1921 gaagaggatc tgaatgctgt gggccaggac acgcaggagg tcatcgtggt gccacactcc
     1981 ttgcccttta aggtggtggt gatctcagcc atcctggccc tggtggtgct caccatcatc
     2041 tcccttatca tcctcatcat gctttggcag aagaagccac gttagGCGGC CGCTCGATAA
     2101 GCTTGATATC GAATTCCGCC CCcccccccT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC
     2161 GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC
     2221 CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA
     2281 GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG
     2341 TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA
     2401 ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG
     2461 CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT
     2521 GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA
     2581 TGGGATCTGA TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA
     2641 ACGTCTAGGC CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA
     2701 TGGCCACAAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
     2761 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
     2821 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
     2881 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
     2941 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
     3001 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
     3061 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
     3121 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
     3181 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
     3241 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
     3301 CCGGTGCCTG ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCACGACCCC
     3361 ATGCATCGAT GATCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA
     3421 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC
     3481 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG
     3541 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT
     3601 GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCTGCAGCCC AAGCTTGGCG
     3661 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
     3721 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
     3781 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCGGATC
     3841 CGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA
     3901 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG
     3961 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG
     4021 GCCTAGGCTT TTGCAAAAAG CTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
     4081 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
     4141 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCCGC TGCATTAATG AATCGGCCAA
     4201 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     4261 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     4321 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     4381 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     4441 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     4501 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     4561 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC
     4621 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     4681 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     4741 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     4801 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
     4861 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     4921 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     4981 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     5041 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     5101 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     5161 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     5221 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     5281 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
     5341 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     5401 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     5461 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     5521 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     5581 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     5641 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     5701 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     5761 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     5821 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     5881 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     5941 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     6001 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATGACCGCTT
     6061 TGGAAAAACA AAGACTGTAT TTCCTGGAAA TTAATGTTTA TTCAATAAAC TGTGTATTCA
     6121 GCTATATCAC ATAGTGGTGA GGCTGAAATG AGGCGGGAAG AGGCGGTTGG GGCTTAATTA
     6181 TATCAATTTG GGTGGCCCCA CAGCGCCTCC AAGGCGCCAG TCCTGTTTTG ACAAGTTGCC
     6241 TCTGGAAGCC TCTACAATGC CTCTCTTCTT TTTCTCCAGA GTAAGCGGAG GCCAGGGGCC
     6301 CCCGGCCTCT GCTTAATACT AAAAAAAAC
//