LOCUS       attB53-Pac-tetO-mCherry 5107 bp ds-DNA     circular     06-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_signal     359..454
                     /label="AttL1"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     misc_signal     478..530
                     /label="AttB53F"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             543..1142
                     /label="Pac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     exon            543..1142
                     /label="Translation 543-1142"
     misc_feature    717..736
                     /label="MMS110_Pac200F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     polyA_signal    1229..1451
                     /label="bGHpA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     1471..1491
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1516..2046)
                     /label="beta-globin polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2076..2786)
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    complement(2819..3228)
                     /label="CMV-TetO"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_signal     3268..3320
                     /label="Att53BF"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_signal     complement(3339..3434)
                     /label="AttL2"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     CDS             3558..4364
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            3558..4364
                     /label="Translation 3558-4364"
     misc_feature    complement(3560..3581)
                     /label="MMS112_KanR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      4457..5044
                     /label="pUC19 ori"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTGGC GACGGATCGG GAGATCTCCG
      481 CGGTGCGGGT GCCAGGGCGT GCCCTTGGGC TCCCCGGGCG CGTACTCCAC CACGCCGCCA
      541 CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG
      601 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG
      661 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG
      721 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG
      781 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG
      841 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG
      901 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG
      961 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC
     1021 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG
     1081 CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT
     1141 GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA
     1201 TGATCTAGAG CTCGCTGATC AGCCTCGACT GTGCTTTCTA GTTGCCAGCC ATCTGTTGTT
     1261 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA
     1321 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG
     1381 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG
     1441 GTGGGCTCTA TGGCTTCTga tatcGGATCC CAGGAAACAG CTATGACCAT GATTACGCCA
     1501 AGCTTGGGCT GCAGGTCGAG GGATCTCCAT AAGAGAAGAG GGACAGCTAT GACTGGGAGT
     1561 AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA AGGAAAATTT TAGGGATGTT
     1621 AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT AACCTCAAGT CAAGGCTTTT
     1681 CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT ACTGATGACC TCTTTATAGC
     1741 CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC
     1801 TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA
     1861 AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC
     1921 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT
     1981 TTGTGAGCCA GTTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG
     2041 TGAATTCGAT ATCAAGCTTA TCGAGCGGCC GCTCAtcact tgtacagctc gtccatgccg
     2101 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
     2161 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
     2221 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
     2281 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
     2341 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
     2401 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
     2461 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
     2521 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
     2581 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
     2641 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     2701 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
     2761 ttatcctcct cgcccttgct caccatGGTG GCGGCCTCGA GTGGCGCGCC GGCCGGCCCG
     2821 GAGGCTGGAT CGGTCCCGGT GTCTTCTATG GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA
     2881 GGCGATCTGA CGGTTCACTA AACGAGCTCT GCTTATATAG GGGGTACCTT CTCTATCACT
     2941 GATAGGGAGC TCGAGACGTG TCGACTTCTC TATCACTGAT AGGGAGACGT TCTAGACGTA
     3001 CCTTCTCTAT CACTGATAGG GAGCTCGAGA CGTGTCGACT TCTCTATCAC TGATAGGGAG
     3061 ACGTTCTAGA CGTACCTTCT CTATCACTGA TAGGGAGCTC GAGACGTGTC GACTTCTCTA
     3121 TCACTGATAG GGAGACGTTC TAGACGTACC TTCTCTATCA CTGATAGGGA GCTCGAGACG
     3181 TGTCGACTTC TCTATCACTG ATAGGGAGAC GTGCGGCCAG CTTGGATCAC TAcgcgccag
     3241 tgctccgaga cggaattcga tatcGTGCCG CGGTGCGGGT GCCAGGGCGT GCCCTTGGGC
     3301 TCCCCGGGCG CGTACTCCAC TGCGCATTAA TCTAGACCCA GCTTTCTTGT ACAAAGTTGG
     3361 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA
     3421 TAAAATCATT ATTTGCCATC CAGCTGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT
     3481 ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA
     3541 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT
     3601 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     3661 GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     3721 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG
     3781 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     3841 TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     3901 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     3961 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
     4021 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     4081 AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     4141 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     4201 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     4261 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     4321 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA
     4381 ATTGGTTGTA ACATTATTCA GATTGGGCCC CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     4441 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     4501 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     4561 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
     4621 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     4681 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     4741 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     4801 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     4861 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     4921 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     4981 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     5041 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     5101 ACATGTT
//