LOCUS       attB53-Pac-TRE-3xFlag-N 4667 bp ds-DNA     circular     06-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_signal     359..454
                     /label="AttL1"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     misc_signal     478..530
                     /label="AttB53F"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             543..1142
                     /label="Pac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     exon            543..1142
                     /label="Translation 543-1142"
     misc_feature    717..736
                     /label="MMS110_Pac200F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     polyA_signal    1229..1451
                     /label="bGHpA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    complement(1472..1602)
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(1620..1636)
                     /label="Neurog1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     exon            complement(1620..1636)
                     /label="Translation 1636-1620"
     CDS             complement(1637..2351)
                     /label="Neurog1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     exon            complement(1637..2351)
                     /label="Translation 2351-1637"
     misc_feature    complement(2352..2369)
                     /label="TGSTGS linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2370..2393)
                     /label="Flag#3 + enterokinase cleavage site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2370..2438)
                     /label="3xFlag"
                     /ApEinfo_revcolor=#e10071
                     /ApEinfo_fwdcolor=#e10071
     exon            complement(2370..2438)
                     /label="Translation 2438-2370"
     misc_feature    complement(2397..2414)
                     /label="Flag#2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2418..2435)
                     /label="Flag#1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(2460..2774)
                     /label="tight TRE promoter"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     misc_signal     2828..2880
                     /label="Att53BF"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_signal     complement(2899..2994)
                     /label="AttL2"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     CDS             3118..3924
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            3118..3924
                     /label="Translation 3118-3924"
     misc_feature    complement(3120..3141)
                     /label="MMS112_KanR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      4017..4604
                     /label="pUC19 ori"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTGGC GACGGATCGG GAGATCTCCG
      481 CGGTGCGGGT GCCAGGGCGT GCCCTTGGGC TCCCCGGGCG CGTACTCCAC CACGCCGCCA
      541 CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG
      601 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG
      661 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG
      721 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG
      781 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG
      841 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG
      901 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG
      961 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC
     1021 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG
     1081 CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT
     1141 GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA
     1201 TGATCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT
     1261 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA
     1321 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG
     1381 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG
     1441 GTGGGCTCTA TGGCTTCTga tatcGGATCC gcagacatga taagatacat tgatgagttt
     1501 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1561 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgCTAG
     1621 TGGTATGGGA TGAAACAGGG CGTCGTGTGG AGCAGGTCTT TGGGCAGGCC AGGAAAGGAG
     1681 AAAAGGGGAT CGCCCGGGCC ATAGGTGAAG TCTTCTGAAG CCGAGGGACT ACTGGGGTCA
     1741 GAGAGTGGTG ATGCCACAGT GGCGCAGGGG GAGGCAGCGG CCCCGGAACC CCAGGACTCA
     1801 GTGTCGCTGG CCGGGCTCGG GGGCCCGGGC AGACAGGGGA CACACTGCGG AGGCAGGAGG
     1861 CGCTCCCGGG CACTGCCCCC GGGGAGCCCT TGATCTGCCA GGCGCAGTGT CTCAGCCAGG
     1921 GCCCAGATGT AGTTGTAGGC GAAGCGCAGC GTCTCAATCT TGGTGAGCTT GGTGTCGTCG
     1981 GGGAACGAGG GCAGCACGCT GCGCAAGGCG TCCAGCGCAG CGTTGAGGTT GTGCATGCGG
     2041 TTGCGCTCGC GATCGTTGGC TTTGACGCGA CGACTCCTCC GCAGGGAGTG CAGCAGAGCC
     2101 TCGGACCGCA CCCGAGCGCG ACCTCGCCGC CTCCGCCGTT CCTGCTCTTC GTCCTGGGCA
     2161 CCGGGAACAT TCGATGCCCC GGAGAGGGCG GGAGCGCTCC TCCGGGCTGG CACGGACAGC
     2221 CCCGAGGTGG AGGCTAGGGG CTGTAGCCTG GCACAGTCCT CCTCGTCGGT GAGGAAGCTG
     2281 GACAGGTCGC TGCTGCTGTT GCTGCTGGAG CAGTCGAGAT CAGAGATGCA GGTCTCCAAA
     2341 GGGGCAGGCA TACTACCTGT ACTACCTGTC TTGTCATCGT CATCCTTGTA ATCGATATCA
     2401 TGATCTTTAT AATCACCGTC ATGGTCTTTG TAGTCCATGG TGGCGGCcat atgtctccag
     2461 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccta
     2521 cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
     2581 gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
     2641 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
     2701 ctcgacatcg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
     2761 tagggagtaa actcgacata tcgattcgaa ggcgcgccag tgctccgaga cggaattcga
     2821 tatcGTGCCG CGGTGCGGGT GCCAGGGCGT GCCCTTGGGC TCCCCGGGCG CGTACTCCAC
     2881 TGCGCATTAA TCTAGACCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT
     2941 TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC
     3001 CAGCTGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT
     3061 ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG
     3121 AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA
     3181 TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG
     3241 TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT
     3301 GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC
     3361 ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA
     3421 AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG
     3481 CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC
     3541 GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG
     3601 AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT
     3661 AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC
     3721 CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA
     3781 GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA
     3841 CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT
     3901 CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACATTATTCA
     3961 GATTGGGCCC CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     4021 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     4081 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     4141 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     4201 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     4261 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     4321 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     4381 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     4441 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     4501 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     4561 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     4621 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
//