LOCUS       pPyCAG-NE-EGFP-IRES-Pac 7342 bp ds-DNA     circular     05-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        1..194
                     /label="PyF101"
                     /ApEinfo_revcolor=#969600
                     /ApEinfo_fwdcolor=#969600
     misc_feature    complement(195..282)
                     /label="Py ori"
                     /ApEinfo_revcolor=#646464
                     /ApEinfo_fwdcolor=#646464
     polyA_signal    365..411
                     /label="SV40 polyA core"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     enhancer        532..837
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        532..2177
                     /label="CAG"
                     /ApEinfo_revcolor=#6f4d6f
                     /ApEinfo_fwdcolor=#6f4d6f
     promoter        838..1113
                     /label="Chiken beta Actin promoter"
                     /ApEinfo_revcolor=#7d0d82
                     /ApEinfo_fwdcolor=#7d0d82
     promoter        1115..2177
                     /label="Rabbit beta globin splice acceptor"
                     /ApEinfo_revcolor=#ac59ff
                     /ApEinfo_fwdcolor=#ac59ff
     promoter        1115..2177
                     /label="CAG promoter"
                     /ApEinfo_revcolor=#ac59ff
                     /ApEinfo_fwdcolor=#ac59ff
     misc_feature    2193..2195
                     /label="ATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_signal     2196..2216
                     /label="NLS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2217..2933
                     /label="eGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2914..2933
                     /label="eGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2934..2957
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2958..3092
                     /label="EMD_TM"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            2958..3092
                     /label="Translation 2958-3092"
     misc_signal     3214..3713
                     /label="IRES"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     mRNA            3725..4324
                     /label="Pac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    4400..4522
                     /label="rabbit betaGlobin polyA core"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     polyA_signal    4411..4633
                     /label="Bovine growth hormone polyadenylationsignal"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     primer_bind     complement(4678..4698)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(4704..4726)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     polyA_signal    5112..5158
                     /label="SV40 polyA core"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     rep_origin      complement(5411..6093)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(6191..6850)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(6191..6850)
                     /label="Translation 6850-6191"
ORIGIN
        1 AGCTGTTGTC ATAGTAATGA TTGGGTGGAA ACATTCCAGG CCTGGGTGGA GAGGCTTTTT
       61 GCTTCCTCTT GCAAAACCAC ACTGACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC
      121 TCTTGCAAAA CCACACTGCC CTCTGGAGGG CAGTTGCCTA GCAACTAACT AAAAGAGGAT
      181 GTCGCACGGC CAGCTGCGGT CAGTTAGTCA CTTCCTGCTT AACTGACTTG ACATTTTCTA
      241 TTTTAAGAGT CGGGAGGAAA ATTACTGTGT TGGAGGCCCT CCGCCATCTT CTGAAGCTGA
      301 ATCGAATTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
      361 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
      421 ATGTATCTTA TCATGTCTGG ATCTGATATC ATCGTCGACA TTGATTATTG ACTAGTTATT
      481 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT
      541 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
      601 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG
      661 ACTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
      721 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT
      781 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGGTC
      841 GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT
      901 TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGCG
      961 CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC
     1021 GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG
     1081 GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG
     1141 TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC GCCCCGGCTC TGACTGACCG CGTTACTCCC
     1201 ACAGGTGAGC GGGCGGGACG GCCCTTCTCC TCCGGGCTGT AATTAGCGCT TGGTTTAATG
     1261 ACGGCTCGTT TCTTTTCTGT GGCTGCGTGA AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT
     1321 GTGCGGGGGG GAGCGGCTCG GGGGGTGCGT GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT
     1381 GCGGCCCGCG CTGCCCGGCG GCTGTGAGCG CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT
     1441 CCGCGTGTGC GCGAGGGGAG CGCGGCCGGG GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG
     1501 AGGGGAACAA AGGCTGCGTG CGGGGTGTGT GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG
     1561 CGGCGGTCGG GCTGTAACCC CCCCCTGCAC CCCCCTCCCC GAGTTGCTGA GCACGGCCCG
     1621 GCTTCGGGTG CGGGGCTCCG TGCGGGGCGT GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG
     1681 TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG
     1741 GAGGGGCGCG GCGGCCCCGG AGCGCCGGCG GCTGTCGAGG CGCGGCGAGC CGCAGCCATT
     1801 GCCTTTTATG GTAATCGTGC GAGAGGGCGC AGGGACTTCC TTTGTCCCAA ATCTGGCGGA
     1861 GCCGAAATCT GGGAGGCGCC GCCGCACCCC CTCTAGCGGG CGCGGGCGAA GCGGTGCGGC
     1921 GCCGGCAGGA AGGAAATGGG CGGGGAGGGC CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT
     1981 CTCCATCTCC AGCCTCGGGG CTGCCGCAGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA
     2041 GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC
     2101 ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC
     2161 ATTTTGGCAA AGAATTCCTC GAGGCCGCCA CCATGCCAAA AAAGAAGAGA AAGGTAATGG
     2221 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
     2281 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
     2341 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
     2401 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
     2461 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
     2521 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
     2581 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
     2641 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
     2701 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
     2761 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
     2821 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
     2881 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGCCG
     2941 GTAGCGGCAG CGGTAGCCGT GCTCCTGGGG CTGGGCTGGG CCAGGATCGC CAGGTCCCGC
     3001 TCTGGGGCCA GCTGCTGCTT TTCCTGGTCT TTGTGATCGT CCTCTTCTTC ATTTACCACT
     3061 TCATGCAGGC TGAAGAAGGC AACCCCTTCT AACCTAGGGC GGCCGCTCGA TAAGCTTGAT
     3121 ATCGAATTCC GCCCCccccc ccTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG
     3181 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
     3241 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT
     3301 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT
     3361 GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC
     3421 ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC CTGCAAAGGC
     3481 GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA AATGGCTCTC
     3541 CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT GTATGGGATC
     3601 TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA
     3661 GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC
     3721 AACCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC
     3781 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC
     3841 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT
     3901 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC
     3961 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG
     4021 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA
     4081 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT
     4141 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT
     4201 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC
     4261 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC
     4321 CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC
     4381 GATGATCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
     4441 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT
     4501 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG
     4561 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG
     4621 CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCTGCAG CCCAAGCTTG GCGTAATCAT
     4681 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
     4741 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
     4801 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCGG ATCCGCATCT
     4861 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
     4921 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
     4981 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
     5041 CTTTTGCAAA AAGCTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
     5101 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
     5161 CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC CAACGCGCGG
     5221 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
     5281 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
     5341 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     5401 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     5461 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     5521 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     5581 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT
     5641 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     5701 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     5761 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     5821 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG
     5881 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     5941 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     6001 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     6061 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     6121 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     6181 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     6241 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     6301 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
     6361 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     6421 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     6481 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     6541 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     6601 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     6661 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     6721 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     6781 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     6841 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     6901 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     6961 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     7021 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATGACCG CTTTGGAAAA
     7081 ACAAAGACTG TATTTCCTGG AAATTAATGT TTATTCAATA AACTGTGTAT TCAGCTATAT
     7141 CACATAGTGG TGAGGCTGAA ATGAGGCGGG AAGAGGCGGT TGGGGCTTAA TTATATCAAT
     7201 TTGGGTGGCC CCACAGCGCC TCCAAGGCGC CAGTCCTGTT TTGACAAGTT GCCTCTGGAA
     7261 GCCTCTACAA TGCCTCTCTT CTTTTTCTCC AGAGTAAGCG GAGGCCAGGG GCCCCCGGCC
     7321 TCTGCTTAAT ACTAAAAAAA AC
//