LOCUS       pGJJ001_SNPsaurus_corre 6304 bp ds-DNA     circular     04-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature        910..1498
                         /label="ori"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     misc_feature        1838..1870
                         /label="gene-specific-adapter-fwd"
                         /ApEinfo_revcolor=#b7e6d7
                         /ApEinfo_fwdcolor=#b7e6d7
     misc_feature        1865..1884
                         /label="barcode landing pad"
                         /ApEinfo_revcolor=#7ea7d8
                         /ApEinfo_fwdcolor=#7ea7d8
     primer binding site complement(1885..1911)
                         /label="gene-specific-adapter-rev"
                         /ApEinfo_revcolor=#85dae9
                         /ApEinfo_fwdcolor=#85dae9
     promoter            1952..2240
                         /label="CYCprom"
                         /ApEinfo_revcolor=#b4abac
                         /ApEinfo_fwdcolor=#b4abac
     CDS                 2256..2498
                         /label="DHFR3"
                         /ApEinfo_revcolor=#d6b295
                         /ApEinfo_fwdcolor=#d6b295
     CDS                 2256..2561
                         /label="Translation 2256-2561"
     CDS                 2499..2558
                         /label="Linker_prey"
                         /ApEinfo_revcolor=#9eafd2
                         /ApEinfo_fwdcolor=#9eafd2
     terminator          2568..2807
                         /label="CYCterm"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     CDS                 complement(2814..2876)
                         /label="Translation 2876-2814"
     CDS                 complement(2817..2876)
                         /label="Linker_bait"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
     CDS                 complement(2877..3200)
                         /label="DHFR12"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
     CDS                 complement(2877..3200)
                         /label="Translation 3200-2877"
     promoter            complement(3216..3504)
                         /label="CYCp"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     misc_feature        3672..4100
                         /label="f1 ori"
                         /ApEinfo_revcolor=#b1ff67
                         /ApEinfo_fwdcolor=#b1ff67
     CDS                 complement(4258..5061)
                         /label="URA3"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
     CDS                 complement(4258..5061)
                         /label="Translation 5061-4258"
     misc_feature        complement(4549..4549)
                         /label="A>G"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     misc_feature        4550..4568
                         /label="!!! Does not amplify with oGJJ110"
                         /ApEinfo_revcolor=#85dae9
                         /ApEinfo_fwdcolor=#85dae9
     PR                  complement(5062..5280)
                         /label="URA3 PR"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     misc_feature        5539..6041
                         /label="CEN/ARS"
                         /ApEinfo_revcolor=#9eafd2
                         /ApEinfo_fwdcolor=#9eafd2
     PR                  6078..6182
                         /label="AmpR PR"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     CDS                 join(6183..6304,1..739)
                         /label="AmpR"
                         /ApEinfo_revcolor=#ff9ccd
                         /ApEinfo_fwdcolor=#ff9ccd
     CDS                 join(6183..6304,1..739)
                         /label="Translation 6183-739"
ORIGIN
        1 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
       61 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      121 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      181 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      241 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      301 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      361 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      421 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      481 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      541 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      601 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      661 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      721 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      781 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      841 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1021 aactggcttc agcagagcgc agataccaaa tactgtTctt ctagtgtagc cgtagttagg
     1081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1561 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1621 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1681 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1741 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttaGct
     1801 cactcattag gcaccccagg ctttacactt tatgcttccg gctcGtatgt tgtgtggaat
     1861 tgtgCGTACG ACTAAGAGCC TAGGcagcta tgaccatgat tacgccaagc gcgcaattaa
     1921 ccctcactaa agggaacaaa agctggagct cATTTGGCGA GCGTTGGTTG GTGGATCAAG
     1981 CCCACGCGTA GGCAATCCTC GAGCAGATCC GCCAGGCGTG TATATATAGC GTGGATGGCC
     2041 AGGCAACTTT AGTGCTGACA CATACAGGCA TATATATATG TGTGCGACGA CACATGATCA
     2101 TATGGCATGC ATGTGCTCTG TATGTATATA AAACTCTTGT TTTCTTCTTT TCTCTAAATA
     2161 TTCTTTCCTT ATACATTAGG ACCTTTGCAG CATAAATTAC TATACTTCTA TAGACACGCA
     2221 AACACAAATA CACACACTAA cggggctgct ctagaatgAG TAAAGTAGAC ATGGTTTGGA
     2281 TAGTCGGAGG CAGTTCTGTT TACCAGGAAG CCATGAATCA ACCAGGCCAC CTCAGACTCT
     2341 TTGTGACAAG GATCATGCAG GAATTTGAAA GTGACACGTT TTTCCCAGAA ATTGAcTTGG
     2401 GGAAATATAA ACTTCTCCCA GAATACCCAG GCGTCCTCTC TGAGGTCCAG GAGGAAAAAG
     2461 GCATCAAGTA TAAGTTTGAA GTCTACGAGA AGAAAGACGG CGGTGGCGGG TCTGGTGGAG
     2521 GAGGGTCAGG TGGTGGCGGC TCGGGAGGTG GAGCTAGCTA Aaagcttatt agttatgtca
     2581 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     2641 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     2701 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     2761 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgact agtTTAGGAT
     2821 CCGCCTCCAC CTGACCCACC TCCTCCAGAC CCGCCTCCGC CTGACCCACC ACCTCCGGTA
     2881 CCCAATTCCG GTTGTTCAAT AAGTCTTAAG GCATCATCCA AACTTTTGGC AAGAAAATGA
     2941 GCTCCTCGTG GTGGTTCTTT GAGTTCTCTA CTGAGAACTA TATTAATTCT GTCCTTTAAA
     3001 GGTCGATTCT TCTCAGGAAT GGAGAACCAG GTTTTCCTAC CCATAATCAC CAGATTCTGT
     3061 TTACCTTCCA CTGAAGAGGT TGTGGTCATT CTTTGGAAGT ACTTGGACTC GTTCCTGAGC
     3121 GGAGGCCAGG GATAGTCTCC GTTCTTGCCA ATCCCCATAT TTTGGGACAC GGCGACGATG
     3181 CAGTTCAATG GTCGAACcat tctagagcag ccccgTTAGT GTGTGTATTT GTGTTTGCGT
     3241 GTCTATAGAA GTATAGTAAT TTATGCTGCA AAGGTCCTAA TGTATAAGGA AAGAATATTT
     3301 AGAGAAAAGA AGAAAACAAG AGTTTTATAT ACATACAGAG CACATGCATG CCATATGATC
     3361 ATGTGTCGTC GCACACATAT ATATATGCCT GTATGTGTCA GCACTAAAGT TGCCTGGCCA
     3421 TCCACGCTAT ATATACACGC CTGGCGGATC TGCTCGAGGA TTGCCTACGC GTGGGCTTGA
     3481 TCCACCAACC AACGCTCGCC AAATgagctc cagcttttgt tccctttagt gagcgtcgtg
     3541 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3661 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3781 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3961 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4021 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4081 acaaaaattt aacgcgaatt ttaacaaaat attaacgCtt acaatttcct gatgcggtat
     4141 tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta
     4201 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     4261 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     4321 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     4381 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     4441 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     4501 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     4561 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     4621 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     4681 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     4741 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     4801 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     4861 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     4921 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     4981 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     5041 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
     5101 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     5161 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     5221 aaaatttcaa Ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaAAg
     5281 gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5341 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5401 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5461 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5521 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
     5581 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
     5641 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa taaacaaagg
     5701 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
     5761 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
     5821 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
     5881 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
     5941 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
     6001 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
     6061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6301 cacg
//