LOCUS       Exported               12810 bp DNA     circular SYN 04-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..243
                     /label=modified 7SK promoter
                     /note="label=7SK promoter - 1..243"
     misc_RNA        265..340
                     /label=guide RNA scaffold for the Streptococcus pyogen...
                     /note="label=gRNA scaffold - note=""guide RNA scaffold for 
                     the Streptococcus pyogenes  - Cas9 system"" - 265..340"
     promoter        402..613
                     /label=EF1-alpha
                     /note="label=EF-1-alpha core promoter - note=""core 
                     promoter for human elongationfactorEF-1-alpha"" - 402..613"
     CDS             638..4741
                     /label=Cas9
                     /note="codon_start=1 - product=""Cas9 (Csn1) endonuclease 
                     from the Streptococcus  - Cas system"" - label=Cas9 - 
                     note=""generates RNA-guided double strand breaks in DNA"" -
                     translation=""MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                      - NLIGALLFDSGETAEATRLKRTARRRYTRRKNR"
     CDS             4742..4789
                     /label=nucleoplasmin NLS
                     /note="codon_start=1 - product=""bipartite nuclear 
                     localization signal from  - nucleoplasmin"" - 
                     label=nucleoplasmin NLS - translation=""KRPAATKKAGQAKKKK"" 
                     - 9219..9266"
     CDS             4790..4813
                     /label=FLAG
                     /note="codon_start=1 - product=""FLAG(R) epitope tag, 
                     followed by an enterokinase  - cleavage site"" - label=FLAG
                     - translation=""DYKDDDDK"" - 9267..9290"
     CDS             4823..4879
                     /label=P2A
                     /note="codon_start=1 - product=""2A peptide from porcine 
                     teschovirus-1  - polyprotein"" - label=P2A - 
                     note=""Eukaryotic ribosomes fail to insert a peptide bond  
                     - between the Gly and Pro residues, yielding separate  - 
                     polypeptides."" - translation=""ATNFSLLKQAGDVEENP"
     CDS             4880..5275
                     /label=BSD
                     /note="codon_start=1 - gene=""Aspergillus terreus BSD"" - 
                     product=""blasticidin S deaminase"" - label=BSD - 
                     note=""confers resistance to blasticidin"" - 
                     translation=""AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                      - NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDL"
     misc_feature    5291..5879
                     /label=woodchuck hepatitis virus posttranscriptional _...
                     /note="label=WPRE - note=""woodchuck hepatitis virus 
                     posttranscriptional  - regulatory element"" - 9768..10356"
     CDS             complement(5762..5773)
                     /label=Factor Xa site
                     /note="codon_start=1 - product=""Factor Xa recognition and 
                     cleavage site"" - label=Factor Xa site - 
                     translation=""IEGR"" - complement(10239..10250)"
     LTR             5951..6184
                     /label=self-inactivating 3' long terminal repeat(LTR)f...
                     /note="label=3' LTR (Delta-U3) - note=""self-inactivating 
                     3' long terminal repeat(LTR)from  - HIV-1"" - 6275..6508"
     LTR             5951..6184
                     /label=self-inactivating 3' long terminal repeat (LTR)...
                     /note="label=3' LTR (Delta-U3) - note=""self-inactivating 
                     3' long terminal repeat (LTR) from - HIV-1"" - 
                     10428..10661"
     LTR             6004..6184
                     /label=truncated 5' long terminal repeat (LTR) from HI...
                     /note="label=5' LTR (truncated) - note=""truncated 5' long 
                     terminal repeat (LTR) from HIV-1"" - 839..1019"
     primer_bind     complement(6210..6227)
                     /label=Bovine growth hormone terminator, reverseprimer...
                     /note="label=BGH-rev - note=""Bovine growth hormone 
                     terminator, reverseprimer.Also - called BGH reverse"" - 
                     complement(6534..6551)"
     primer_bind     complement(6210..6227)
                     /label=Bovine growth hormone terminator, reverse prime...
                     /note="label=BGH-rev - note=""Bovine growth hormone 
                     terminator, reverse primer.  - Also called BGH reverse"" - 
                     complement(10687..10704)"
     polyA_signal    6216..6440
                     /label=bovine growth hormone polyadenylation signal_bG...
                     /note="label=bGH poly(A) signal - note=""bovine growth 
                     hormone polyadenylation signal"" - 10693..10917"
     rep_origin      6486..6914
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of _f1 ori
                     /note="direction=RIGHT - label=f1 ori - note=""f1 
                     bacteriophage origin of  - replication;arrowindicates 
                     direction of (+) strand  - synthesis"" - 6810..7238"
     rep_origin      6486..6914
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication; arrow _...
                     /note="direction=RIGHT - label=f1 ori - note=""f1 
                     bacteriophage origin of replication; arrow  - indicates 
                     direction of (+) strand synthesis"" - 10963..11391"
     primer_bind     complement(6573..6592)
                     /label=F1 origin, reverse primer_F1ori-R
                     /note="label=F1ori-R - note=""F1 origin, reverse primer"" -
                     complement(11050..11069)"
     primer_bind     6783..6804
                     /label=F1 origin, forward primer_F1ori-F
                     /note="label=F1ori-F - note=""F1 origin, forward primer"" -
                     11260..11281"
     primer_bind     complement(6923..6943)
                     /label=SV40 enhancer, reverse primer for pBABE vectors...
                     /note="label=pBABE 3' - note=""SV40 enhancer, reverse 
                     primer for pBABE vectors"" - complement(11400..11420)"
     promoter        6928..7257
                     /label=SV40 enhancer and early promoter_SV40 promoter
                     /note="label=SV40 promoter - note=""SV40 enhancer and early
                     promoter"" - 11405..11734"
     rep_origin      7108..7243
                     /direction=RIGHT
                     /label=SV40 origin of replication_SV40 ori
                     /note="label=SV40 ori - note=""SV40 origin of replication""
                     - 11585..11720"
     primer_bind     7170..7189
                     /label=SV40 promoter/origin, forward primer_SV40pro-F
                     /note="label=SV40pro-F - note=""SV40 promoter/origin, 
                     forward primer"" - 11647..11666"
     promoter        7305..7352
                     /label=synthetic bacterial promoter _EM7 promoter
                     /note="label=EM7 promoter - note=""synthetic bacterial 
                     promoter "" - 11782..11829"
     CDS             7371..7745
                     /label=confers resistance to bleomycin, phleomycin, an...
                     /note="codon_start=1 - gene=""Sh ble from 
                     Streptoalloteichus hindustanus"" - 
                     product=""antibiotic-binding protein"" - label=BleoR - 
                     note=""confers resistance to bleomycin, phleomycin, and  - 
                     Zeocin(TM)"" - 
                     translation=""MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                      - V"
     polyA_signal    7875..7996
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /note="label=SV40 poly(A) signal - note=""SV40 
                     polyadenylation signal"" - 12352..12473"
     rep_origin      complement(8447..9035)
                     /direction=LEFT
                     /label=ColE1
                     /note="direction=LEFT - label=ori - note=""high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  - replication"" - 
                     complement(12924..13512)"
     CDS             complement(9206..10066)
                     /label=bla
                     /note="codon_start=1 - gene=""bla"" - 
                     product=""beta-lactamase"" - label=AmpR - note=""confers 
                     resistance to ampicillin,carbenicillin,and  - related 
                     antibiotics"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                      - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQE"
     promoter        complement(10067..10171)
                     /label=AmpR promoter
                     /note="gene=""bla"" - label=AmpR promoter - 
                     complement(14544..14648)"
     promoter        10822..11020
                     /label=CMV_IE
                     /note="label=CMV promoter - note=""human cytomegalovirus 
                     (CMV) immediateearlypromoter"" - 11142..11340"
     promoter        10822..11020
                     /label=human cytomegalovirus (CMV) immediate early _CM...
                     /note="label=CMV promoter - note=""human cytomegalovirus 
                     (CMV) immediate early  - promoter"" - 623..821"
     LTR             11038..11218
                     /label=truncated 5' long terminal repeat (LTR) from HI...
                     /note="label=5' LTR (truncated) - note=""truncated 5' long 
                     terminal repeat (LTR) from HIV-1"" - 839..1019"
     misc_feature    11265..11390
                     /label=Psi
                     /note="label=HIV-1 Psi - note=""packaging signal of human 
                     immunodeficiency virus  - type 1"" - 1066..1191"
     misc_feature    11883..12116
                     /label=RRE
                     /note="label=RRE - note=""The Rev response element (RRE) of
                     HIV-1 allows for  - Rev-dependent mRNA export from the 
                     nucleus to the  - cytoplasm."" - 1684..1917"
     misc_feature    12643..12760
                     /label=central polypurine tract and _cPPT/CTS
                     /note="label=cPPT/CTS - note=""central polypurine tract and
                      - centralterminationsequence of HIV-1"" - 12963..13080"
ORIGIN
        1 ctgcagtatt tagcatgccc cacccatctg caaggcattc tggatagtgt caaaacagcc
       61 ggaaatcaag tccgtttatc tcaaacttta gcattttggg aataaatgat atttgctatg
      121 ctggttaaat tagattttag ttaaatttcc tgctgaagct ctagtacgat aagcaacttg
      181 acctaagtgt aaagttgaga cttccttcag gtttatatag cttgtgcgcc gcttgggtac
      241 accggagacg gttaacacgt ctctgtttta gagctagaaa tagcaagtta aaataaggct
      301 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta
      361 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      421 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
      481 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      541 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      601 cgccagaaca caggaccggt tctagagcgc tgccaccatg gacaagaagt acagcatcgg
      661 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      721 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
      781 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
      841 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
      901 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
      961 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     1021 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     1081 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     1141 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     1201 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     1261 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     1321 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     1381 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     1441 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     1501 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     1561 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     1621 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     1681 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     1741 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     1801 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     1861 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     1921 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     1981 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     2041 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     2101 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     2161 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     2221 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     2281 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     2341 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     2401 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     2461 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     2521 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     2581 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     2641 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     2701 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     2761 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     2821 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     2881 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     2941 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     3001 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     3061 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     3121 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     3181 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     3241 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     3301 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     3361 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     3421 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     3481 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     3541 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     3601 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     3661 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     3721 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     3781 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     3841 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     3901 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     3961 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     4021 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     4081 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     4141 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     4201 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     4261 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     4321 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     4381 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     4441 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     4501 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     4561 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     4621 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     4681 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     4741 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     4801 cgatgacgat aagggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     4861 cgaagagaat cctggaccgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     4921 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     4981 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     5041 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     5101 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     5161 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     5221 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaaacgcg
     5281 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5341 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5401 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5461 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5521 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5581 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5641 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5701 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5761 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5821 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     5881 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5941 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6181 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6241 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6301 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6361 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6421 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6481 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6901 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7081 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7141 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     7201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7261 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     7321 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     7381 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     7441 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     7501 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     7561 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     7621 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     7681 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     7741 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7801 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7861 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7921 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7981 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     8041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10201 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    10261 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    10321 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    10381 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgcgcgtt
    10441 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    10501 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    10561 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    10621 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    10681 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    10741 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    10801 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
    10861 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
    10921 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
    10981 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
    11041 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    11101 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    11161 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    11221 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    11281 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    11341 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    11401 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    11461 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    11521 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    11581 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    11641 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    11701 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
    11761 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    11821 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11881 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11941 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    12001 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    12061 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    12121 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    12181 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    12241 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    12301 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12361 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12421 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12481 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    12541 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
    12601 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
    12661 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    12721 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    12781 cagagatcca gtttggttaa ttaaggtacc
//
