LOCUS       pREC143                11864 bp ds-DNA     circular     05-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1864..2044
                     /label="LTR (Lenti)"
                     /ApEinfo_revcolor=#8458a7
                     /ApEinfo_fwdcolor=#8458a7
     primer          3433..3457
                     /label="HK179"
                     /note="sequence: cgccaaatggcagtattcatccaca"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     repeat_region   3433..3612
                     /label="cPPT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3577..3601
                     /label="HK371"
                     /note="sequence: cgggtttattacagggacagcagag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3588..3611
                     /label="cPPT_5'seq"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3621..3662
                     /label="HK854"
                     /note="sequence: gcttgatatcgaattttttaactagactagtcggttagcttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(3652..3674)
                     /label="amplified w/ sv40"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3675..3806)
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3695..3721
                     /label="HK615"
                     /note="sequence: cattgatgagtttggacaaaccacaac"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3808..3853
                     /label="sg256"
                     /note="sequence: TCGAGAAACAAAAAGAAAACTCGATCTACACTTAGTAGAAATTtac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3812..3859)
                     /label="sg257"
                     /note="sequence: acaagggtaAATTTCTACTAAGTGTAGATCGAGTTTTCTTTTTGTTTC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(3831..3850)
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3851..3873)
                     /label="crGFP* ABE3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3854..3905
                     /label="sg258"
                     /note="sequence: ccttgttaaCTGAatcgagtATCTACACTTAGTAGAAATTtacccttgttaa"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3860..3911)
                     /label="sg259"
                     /note="sequence: atTCAGttaacaagggtaAATTTCTACTAAGTGTAGATactcgatTCAGtta"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(3874..3893)
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3894..3916)
                     /label="crGFP* ABE3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3906..3949
                     /label="sg260"
                     /note="sequence: CTGAatcgagtatctacacttagtagaaattCAAAGAAAGAAAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3912..3953)
                     /label="sg261"
                     /note="sequence: GATCCTTTCTTTCTTTGaatttctactaagtgtagatactcg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3917..3936)
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     polyA_signal    4005..4114
                     /label="Triplex"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     CDS             complement(4121..4819)
                     /label="TagBFP"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     primer          complement(4361..4389)
                     /label="HK366"
                     /note="sequence: AGACtCTGTAtCCtGCTGACGGCGGCCTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          4372..4404
                     /label="HK365"
                     /note="sequence: CAGCaGGaTACAGaGTCTCGGTGAAGGCCTCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(4526..4545)
                     /label="oTG_so4"
                     /note="sequence: TCACCACATACGAAGACGGG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(4526..4545)
                     /label="oTG_s04"
                     /note="sequence: tcaccacatacgaagacggg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4552..4579
                     /label="HK923"
                     /note="sequence: CCCATGTGAAGCCCTCAGGGAAGGACTG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4748..4770
                     /label="HK360"
                     /note="sequence: cttgaagtgatggttgtccacgg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          4813..4849
                     /label="HK954"
                     /note="sequence: GCTCGCTcatgaattcAAGCTTTCTGGgTAGTAGACT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4852..4910)
                     /label="Chen minCMV"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          4914..4948
                     /label="HK969"
                     /note="sequence: TATGtcgagcccgggGAGTCTAGATCTACGCCTTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(4941..5030)
                     /label="NFAT response elements"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(5031..5070)
                     /label="HK970"
                     /note="sequence: gctctgcccgtcgacATATggatTACCTGAGCTCGCTAGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        5062..5273
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5119..5140
                     /label="HK873"
                     /note="sequence: gatccggtgcctagagaaggtg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5157..5180)
                     /label="HK698"
                     /note="sequence: gagccagtacacgacatcactttc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             5284..6306
                     /label="HygR (EcoRI/NdeI removed)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5504..5534)
                     /label="HK938"
                     /note="sequence: ctgaaCtccccaatgtcaagcacttccggaa"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5518..5552
                     /label="HK939"
                     /note="sequence: gacattggggaGttcagcgagagcctgacctattg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5529..5529
                     /label="edited to remove EcoRI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5706..5742)
                     /label="HK940"
                     /note="sequence: cgcgcaGatgaaatcacgccatgtagtgtattgaccg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5726..5758
                     /label="HK941"
                     /note="sequence: gtgatttcatCtgcgcgattgctgatccccatg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5736..5736
                     /label="edited to remove NdeI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6287..6317)
                     /label="HK943"
                     /note="sequence: tcggaaccgccTTCCTTTGCCCTCGGACGAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          6303..6334
                     /label="HK944"
                     /note="sequence: GGAAggcggttccgagggcagaggaagtctgc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6316..6369
                     /label="T2A (EGRGSLLTCGDVEENPG/P)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6382..7128
                     /label="rtTA (no XhoI)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6948..6978)
                     /label="HK870"
                     /note="sequence: cttctcCaggccgcagatgatcagttccagg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6964..6994
                     /label="HK871"
                     /note="sequence: tgcggcctGgagaagcagctgaagtgcgaga"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6972..6972
                     /label="silent mutation to remove xhoi"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(7109..7147)
                     /label="HK872"
                     /note="sequence: atcaagcttgcGTTTAAACttagcctggcagcatatcga"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7137..7161
                     /label="amplify with WPRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7153..7175
                     /label="1167_seq"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     enhancer        7162..7753
                     /label="WPRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7216..7236)
                     /label="QB0112"
                     /note="sequence: catagcgtaaaaggagcaaca"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(7220..7245)
                     /label="HK510"
                     /note="sequence: gcgtatccacatagcgtaaaaggagc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(7334..7355)
                     /label="HK210"
                     /note="sequence: tgacaacgggccacaactcctc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          7641..7662
                     /label="HK095"
                     /note="sequence: TCAATCCAGCGGACCTTCCTTC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(7745..7779)
                     /label="HK859"
                     /note="sequence: tggtcttaaaggtactcgacggtatcatgcgggga"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7765..7790
                     /label="backbone"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    7888..8068
                     /label="LTR (Lenti)"
                     /ApEinfo_revcolor=#6935ca
                     /ApEinfo_fwdcolor=#6935ca
     CDS             9004..9864
                     /label="bla\(AmpR)"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
ORIGIN
        1 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
       61 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
      121 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
      181 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
      241 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
      301 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
      361 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
      421 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
      481 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
      541 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
      601 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
      661 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
      721 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
      781 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
      841 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
      901 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
      961 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     1021 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1081 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1201 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     1261 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg
     1321 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     1381 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     1441 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     1501 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     1561 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     1621 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     1681 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     1741 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     1801 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     1861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2041 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2101 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     2161 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     2221 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     2281 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     2341 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     2401 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     2461 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     2521 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     2581 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     2641 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     2701 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     2761 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     2821 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2881 gggcatcaag cagctccagg caagaatcct ggctgtgaaa gatacctaaa ggatcaacag
     2941 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3001 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3061 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3121 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3181 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     3241 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     3301 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     3361 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     3421 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3601 gatccagttt ggatcgataa gcttgatatc gaatttttta actagactag tcggttagct
     3661 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3721 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3781 taaccattat aagctgcaat aaacaaCTCG AGAAACAAAA AGAAAACTCG ATCTACACTT
     3841 AGTAGAAATT tacccttgtt aaCTGAatcg agtATCTACA CTTAGTAGAA ATTtaccctt
     3901 gttaaCTGAa tcgagtatct acacttagta gaaattCAAA GAAAGAAAGG ATCCtcgcag
     3961 accaatacca ggatcttgcc atcctatgga actgcctcgg tgagTTTTGC TTTTTTTTTT
     4021 TTAAAGCTAG GGAAAGGCCA AAAAGCAAAA CCTGAGAAAA CAAAAGGTTG TTTTCTCAGG
     4081 AAAAGAAAAA CCTTTACAAC CCTACTGACG AATCACGCGT ttaATTAAGC TTGTGCCCCA
     4141 GTTTGCTAGG GAGGTCGCAG TATCTGGCCA CTGCCACCTC GTGCTGCTCG ACGTAGGTCT
     4201 CGTTGTTGGC CTCCTTGATT CTTTCCAGTC TGTAGTCCAC ATAGTAGACG CCAGGCATCT
     4261 TGAGGTTCTT AGCGGGTTTC TTGGATCTAT ATGTGGTCTT GATGTTTGCG ATCAGATGGC
     4321 TCCCGCCCAC GAGCTTCAGG GCCATGTCGT TTCTGCCTTC CAGGCCGCCG TCAGCaGGaT
     4381 ACAGaGTCTC GGTGAAGGCC TCCCAGCCGA GTGTTTTCTT CTGCATCACA GGGCCGTTGG
     4441 ATGTGAAGTT CACCCCTCTG ATCTTGACGT TGTAGATGAG GCAGCCGTCC TGGAGGCTGG
     4501 TGTCCTGGGT AGCGGTCAGC ACGCCCCCGT CTTCGTATGT GGTGACTCTC TCCCATGTGA
     4561 AGCCCTCAGG GAAGGACTGC TTGAAGAAGT CGGGGATGCC CTGGGTGTGG TTGATGAAGG
     4621 TCTTGCTGCC GTAGAGGAAG CTAGTAGCCA GGATGTCGAA GGCGAAGGGG AGAGGGCCGC
     4681 CCTCGACCAC CTTGATTCTC ATGGTCTGGG TGCCCTCGTA GGGCTTGCCT TCGCCCTCGG
     4741 ATGTGCACTT GAAGTGATGG TTGTCCACGG TGCCCTCCAT GTACAGCTTC ATGTGCATGT
     4801 TCTCCTTAAT CAGCTCGCTc atgaattcAA GCTTTCTGGg TAGTAGACTA GGATCTGACG
     4861 GTTCACTAAA CGAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC GCATATGtcg
     4921 agcccgggGA GTCTAGATCT ACGCCTTCTG TATGAAACAG TTTTTCCTCC ACGCCTTCTG
     4981 TATGAAACAG TTTTTCCTCC ACGCCTTCTG TATGAAACAG TTTTTCCTCC GCTAGCGAGC
     5041 TCAGGTAatc cATATgtcga cgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     5101 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     5161 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     5221 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt
     5281 ACCatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     5341 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     5401 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     5461 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     5521 attggggaGt tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     5581 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     5641 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     5701 ggaatcggtc aatacactac atggcgtgat ttcatCtgcg cgattgctga tccccatgtg
     5761 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5821 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5881 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5941 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6001 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     6061 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     6121 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     6181 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     6241 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccaGCACTCG TCCGAGGGCA
     6301 AAGGAAggcg gttccgaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     6361 cctggcccag gcggagggtc gatgagccgg ctggacaaga gcaaagtgat caacagcgcc
     6421 ctggaactgc tgaacggcgt gggcatcgag ggcctgacca cccggaagct ggcccagaaa
     6481 ctgggcgtgg aacagcccac cctgtactgg cacgtgaaga acaagcgggc cctgctggac
     6541 gccctgccca tcgagatgct ggaccggcac cacacccaca gctgccctct ggaaggcgag
     6601 agctggcagg acttcctgcg gaacaacgcc aagagctaca gatgcgccct gctgagccac
     6661 cgggacggcg ccaaagtgca cctgggcacc agacccaccg agaagcagta cgagacactg
     6721 gaaaaccagc tggccttcct gtgccagcag ggcttcagcc tggaaaacgc cctgtacgcc
     6781 ctgagcgccg tgggccactt caccctgggc tgcgtgctgg aagaacagga acaccaggtc
     6841 gccaaagagg aaagagagac acccaccacc gacagcatgc cccccctgct gaagcaggcc
     6901 atcgagctgt tcgacagaca gggcgccgag cccgccttcc tgttcggcct ggaactgatc
     6961 atctgcggcc tGgagaagca gctgaagtgc gagagcggcg gacccaccga cgccctggac
     7021 gacttcgacc tggacatgct gcccgccgat gccctggatg attttgatct ggatatgctc
     7081 cctgctgacg ctctcgatga cttcgatctc gatatgctgc caggctaaGT TTAAACgcaa
     7141 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     7201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7741 cgcctccccg catgataccg tcgagtacct ttaagaccaa tgacttacaa ggcagctgta
     7801 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7861 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8041 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     8101 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     8161 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     8221 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     8281 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     8341 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     8401 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     8461 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     8521 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     8581 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     8641 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     8701 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8761 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     8821 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     8881 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8941 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9001 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9061 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9121 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9181 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9241 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9301 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9361 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9421 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9481 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9541 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9601 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9661 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9721 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9781 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9841 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9901 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9961 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10021 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10081 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10141 aaggtaactg gcttcagcag agcgcagata ccaaatactg tctttctagt gtagccgtag
    10201 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10261 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10321 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10381 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10441 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10501 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10561 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10621 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10681 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10741 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10801 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10861 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10921 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10981 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    11041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    11101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    11161 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
    11221 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
    11281 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
    11341 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
    11401 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
    11461 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
    11521 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
    11581 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
    11641 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
    11701 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
    11761 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
    11821 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaa
//