LOCUS       Exported               10576 bp DNA     circular SYN 15-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..300
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=24.5"
                     /note="/other=CDS"
                     /translation="STDALESLQPSQLLPVGAGHDYRRRTYDCLLYHATRRTGAGSALG
                     HFRRGPLSLERDDDRPVACGIRNLARPRSSLRHWSRHQTFRREAGHYRRHGG"
     CDS             complement(372..752)
                     /codon_start=1
                     /label=YPB1_ECOLX
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=96.2"
                     /note="/other=CDS"
                     /translation="GGFLFMGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRR
                     DQRKITQGQCQRFVNTDVGVPQGSQQHPAMQIRNIMVQGADFRVSRLYETRKPKTIHVV
                     AQVADVLQQQSLHVRSRIGDSFC"
     CDS             769..960
                     /codon_start=1
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1062..1201
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(1387..1975)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             2097..2912
                     /codon_start=1
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(2921..2978)
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=56.3"
                     /note="/other=promoter"
     rep_origin      complement(3031..3459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     terminator      complement(3496..3543)
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(3551..3600)
                     /codon_start=1
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
                     /translation="AANKARKEAELAAATA"
     CDS             complement(3694..7797)
                     /codon_start=1
                     /label=SuperFi-Cas9
                     /label=Cas9 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     IL*GLFYLTVERQRKRLVSNGQLVEGIHVGRIVFVIYRRFFQMRWRK*MIVSFIDLKSL
                     FWWKKTRSMNVILFLEI**MKLLIMRNIQLSIICEKNW*ILLIKRICA*SIWP*RI*LS
                     FVVIF*LREI*ILIIVMWTNYLSSWYKPTINYLKKTLLTQVE*MLKRFFLHD*VNQDD*
                     KISLLSSPVRRKMAYLGISLLCHWV*PLILNQILIWQKMLNYSFQKILTMMI*IIYWRK
                     LEINMLICFWQLRIYQMLFYFQIS*E*ILK*LRLPYQLQ*LNATMNIIKT*LF*KL*FD
                     NNFQKSIKKSFLINQKTDMQVILMGELAKKNFINLSNQF*KKWMVLRNYW*N*IVKICC
                     ASNGPLTTALFPIKFTWVSCMLF*EDKKTFIHF*KTIVRRLKKS*LFEFLIMLVHWRVA
                     IVVLHG*LGSLKKQLPHGILKKLSIKVLQLNHLLNA*QTLIKIFQMKKYYQNIVCFMSI
                     LRFITN*QRSNMLLKECENQHFFQVNRRKPLLIYSSKQIEK*PLSN*KKIISKK*NVLI
                     VLKFQELKIDLMLH*VPTMIC*KLLKIKIFWIMKKMKIS*RILF*H*PYLKIGR*LRKD
                     LKHMLTSLMIR**NSLNVAVILVGDVCLEN*LMVLGISNLAKQY*IF*NQMVLPIAILC
                     S*SMMIV*HLKKTFKKHKCLDKAIVYMNILQI*LVALLLKKVFYRL*KLLMNWSK*WGG
                     ISQKISLLKWHVKIRQLKRARKIRESV*NESKKVSKN*EVRFLKSILLKILNCKMKSSI
                     SIISKMEETCMWTKN*ILIV*VIMMSITLFHKVSLKTIQ*TIRS*RVLIKIVVNRITFQ
                     VKK*SKR*KTIGDNF*TPS*SLNVSLII*RKLNVEV*VNLIKLVLSNANWLKLAKSLSM
                     WHKFWIVA*ILNTMKMINLFERLK*LP*NLN*FLTSEKISNSIKYVRLTITIMPMMRI*
                     MPSLELL*LRNIQNLNRSLSMVMIKLMMMIK*LLSLSKI*AMQPQNISFTLIS*TSSKQ
                     KLHLQMERFANAL*SKLMGKLEKLSGIKGEILPQCAKYCPCPKSILSRKQKYRQADSPR
                     SQFYQKEIRTSLLLVKKTGIQKNMVVLIVQR*LIQS*WLLRWKKGNRRS*NPLKSY*GS
                     QLWKEVPLKKIRLTF*KLKDIRKLKKT*SLNYLNIVFLS*KTVVNGCWLVPENYKKEMS
                     WLCQANM*IFYI*LVIMKS*RVVQKITNKNNCLWSSISII*MRLLSKSVNFLSVLF*QM
                     PI*IKFLVHITNIETNQYVNKQKILFIYLR*RILELPLLLNILIQQLIVNDIRLQKKF*
                     MPLLSINPSLVFMKHALI*VS*EVT"
     CDS             complement(7858..8955)
                     /codon_start=1
                     /label=MBP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     CDS             complement(8959..8976)
                     /codon_start=1
                     /label=6xHis
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="HHHHHH"
     RBS             complement(8987..9009)
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     protein_bind    complement(9024..9048)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(9049..9067)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9089..9368
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
                     /translation="DPLRRTHRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRR
                     GYGGRPRGRGTVGRHLLACTIPCGGGAQRPQPTTGLLPNAGVA*GRAS"
     promoter        9376..9453
                     /label=lacI promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9454..10536
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    10549..10570
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=95.5"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
ORIGIN
        1 ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
       61 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
      121 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
      181 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
      241 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
      301 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
      361 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
      421 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
      481 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
      541 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
      601 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
      661 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
      721 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
      781 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
      841 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
      901 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
      961 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     1021 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     1081 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     1141 gagtgcacca ttgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     1861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2041 tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2101 gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg
     2161 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2221 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2281 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2341 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     2401 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2461 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2521 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2581 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2641 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2701 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2761 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2821 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2881 agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt
     2941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3001 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3061 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat
     3481 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     3541 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     3601 cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt cgacggagct
     3661 cgaattcgga tcctgcaaag caccggcctc gttagtcacc tcctagctga ctcaaatcaa
     3721 tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt
     3781 ttgtagacgt atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag
     3841 ctccaagatt cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt
     3901 tgtctctatg tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa
     3961 cacgcttaga aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct
     4021 ccacaaacaa ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac
     4081 tagctaaata taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta
     4141 attctccggc actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt
     4201 taggtagttt aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt
     4261 caatcggatt tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa
     4321 cggattttaa cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag
     4381 ctaccgttgg actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa
     4441 taagcttgtc cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta
     4501 cttctgtttt cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa
     4561 aatctcgccc tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc
     4621 gtttgcgaat ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag
     4681 agtaaaagaa atattttgcg gttgcatcgc ctatatcttg ctcagactta gcaatcattt
     4741 tatcatcatc atcaacttta tcatcaccat cgacaaactc cgattcaagt tttggatatt
     4801 tcttaatcaa agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat
     4861 tgttaatctc acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag
     4921 attttaaggt aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat
     4981 tcatgcgact atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt
     5041 ggcgtttgat aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta
     5101 aattatcaaa cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt
     5161 tcatcttttt gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag
     5221 aacgcgttaa gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgt
     5281 gatcgacatc ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc
     5341 ttccattttg gagataatag agatagagct tttcattttg caattgagta ttttcaacag
     5401 gatgctcttt aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac
     5461 gctctcgcga atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa
     5521 cgatattttc tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag
     5581 tctgtaaaat acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta
     5641 aactatcgcc ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat
     5701 catcatggat cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta
     5761 atattgtttt gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc
     5821 cccaaccagt ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag
     5881 catatgtttt aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa
     5941 caatatcctc taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa
     6001 tttttagcaa atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa
     6061 tttcaacact atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg
     6121 ttacttttcg atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa
     6181 gaaatgctgg ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa
     6241 ccgtaaaata ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt
     6301 tatcaaagtt tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt
     6361 cttcaaaatt ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac
     6421 tattgccacg cgccaatgga ccaacataat aaggaattcg aaaagtcaag attttttcaa
     6481 tcttctcacg attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat
     6541 gcagctcacc caagtgaatt tgatggggaa tagagccgtt gtcaaaggtc cgttgcttgc
     6601 gcagcaaatc ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta
     6661 aaattggttt gataaattta taaaattctt cttggctagc tcccccatca atataacctg
     6721 catatccgtt ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc
     6781 gaactaaagc ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg
     6841 aagctgatag gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa
     6901 tagcatctga taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg
     6961 ccaataaatt atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt
     7021 ctgccaaatc aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat
     7081 tcccaaataa gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc
     7141 ttgatttact caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag
     7201 ggttttcttc aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat
     7261 cactattatc aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat
     7321 gcgctaaggc caaatagatt aagcgcaaat ccgctttatc agtagaatct accaattttt
     7381 ttcgcagatg atagatagtt ggatatttct catgataagc aacttcatct actatatttc
     7441 caaaaatagg atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc
     7501 gatgaaagaa actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac
     7561 aaatacgatt cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt
     7621 ccgctgtctc tccactgtca aataaaagag cccctataag atttttttga tactgtggcg
     7681 gtctgtattt cccagaacct tgaacttttt agacggaacc ttatattcat cagtgatcac
     7741 cgcccatccg acgctatttg tgccgatatc taagcctatt gagtatttct tatccatcga
     7801 ggctgctccc tggaaataca ggttttcact agtgttgttg ttgttcgagc tcgaattagt
     7861 ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc
     7921 agtacgcacg gcataccaga aagcggacat ctgcgggatg ttcggcatga tttcaccttt
     7981 ctgggcgttt tccatagtgg cggcaatacg tggatctttc gccaactctt cctcgtaaga
     8041 cttcagcgct acggcaccca gcggtttgtc tttattaacc gcttccagac cttcatcagt
     8101 cagcagatag ttttcgagga actcttttgc cagctctttg ttcggactgg cggcgttaat
     8161 acctgcgctc agcacgccaa cgaacggttt ggatggttga cccttgaagg tcggcagtac
     8221 cgttacacca taattcactt tgctggtgtc gatgttggac catgcccacg ggccgttgat
     8281 ggtcatcgct gtttcgcctt tattaaaggc agcttctgcg atggagtaat cggtgtctgc
     8341 attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc agacccgctt tcgcgccagc
     8401 gttatccacg cccacgtctt taatgtcgta cttgccgttt tcatacttga acgcataacc
     8461 cccgtcagca gcaatcagcg gccaggtgaa gtacggttct tgcaggttga acatcagcgc
     8521 gctcttacct ttcgctttca gttctttatc cagcgccggg atctcttccc aggtttttgg
     8581 cgggttcggc agcagatctt tgttataaat cagcgataac gcttcaacag cgatcgggta
     8641 agcaatcagc ttgccgttgt aacgtacggc atcccaggta aacggataca gcttgtcctg
     8701 gaacgctttg tccggggtga tttcagccaa caggccagat tgagcgtagc caccaaagcg
     8761 gtcgtgtgcc cagaagataa tgtcagggcc atcgccagtt gccgcaacct gtgggaattt
     8821 ctcttccagt ttatccggat gctcaacggt gactttaatt ccggtatctt tctcgaattt
     8881 cttaccgact tcagcgagac cgttatagcc tttatcgccg ttaatccaga ttaccagttt
     8941 accttcttcg atttttccat ggtgatggtg atggtgcata tgtatatctc cttcttaaag
     9001 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
     9061 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
     9121 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     9181 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     9241 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     9301 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     9361 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
     9421 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
     9481 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     9541 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     9601 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     9661 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     9721 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     9781 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     9841 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     9901 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     9961 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
    10021 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
    10081 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
    10141 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
    10201 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
    10261 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
    10321 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
    10381 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
    10441 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10501 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta
    10561 gctcactcat taggca
//
