LOCUS       Exported                5787 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
COMMENT     Addgene NGS Result 

Clontech vector
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    365..568
                     /label=CMV promoter
     misc_feature    601..642
                     /label=NES (X. laevis)
     misc_feature    658..1365
                     /label=mCherry
     misc_feature    1402..1920
                     /label=BTK-PH (2-174)
     misc_feature    1936..2442
                     /label=BTK-PH (2-170)
     misc_feature    2443..2445
                     /label=STOP
     misc_feature    3683..4477
                     /label=NeoR/KanR
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggctctgc agaaaaagtt ggaagagctt gagctggatg aggcaccggt cgccaccatg
      661 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      721 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      781 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      841 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      901 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      961 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1021 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1081 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     1141 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1201 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1261 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1321 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatct
     1381 cgagctcaag cttcgaattc tgccgcagtg attctggaga gcatctttct gaagcgatcc
     1441 caacagaaaa agaaaacatc acctctaaac ttcaagaagc gcctgtttct cttgaccgtg
     1501 cacaaactct cctactatga gtatgacttt gaacgtggga gaagaggcag taagaagggt
     1561 tcaatagatg ttgagaagat cacttgtgtt gaaacagtgg ttcctgaaaa aaatcctcct
     1621 ccagaaagac agattccgag aagaggtgaa gagtccagtg aaatggagca aatttcaatc
     1681 attgaaaggt tcccttatcc cttccaggtt gtatatgatg aagggcctct ctacgtcttc
     1741 tccccaactg aagaactaag gaagcggtgg attcaccagc tcaaaaacgt aatccggtac
     1801 aacagtgatc tggttcagaa atatcaccct tgcttctgga tcgatgggca gtatctctgc
     1861 tgctctcaga cagccaaaaa tgctatgggc tgccaaattt tggagaacag gaatggaagc
     1921 gggggttccg gcggagccgc agtgattctg gagagcatct ttctgaagcg atcccaacag
     1981 aaaaagaaaa catcacctct aaacttcaag aagcgcctgt ttctcttgac cgtgcacaaa
     2041 ctctcctact atgagtatga ctttgaacgt gggagaagag gcagtaagaa gggttcaata
     2101 gatgttgaga agatcacttg tgttgaaaca gtggttcctg aaaaaaatcc tcctccagaa
     2161 agacagattc cgagaagagg tgaagagtcc agtgaaatgg agcaaatttc aatcattgaa
     2221 aggttccctt atcccttcca ggttgtatat gatgaagggc ctctctacgt cttctcccca
     2281 actgaagaac taaggaagcg gtggattcac cagctcaaaa acgtaatccg gtacaacagt
     2341 gatctggttc agaaatatca cccttgcttc tggatcgatg ggcagtatct ctgctgctct
     2401 cagacagcca aaaatgctat gggctgccaa attttggaga actgaggatc caccggatct
     2461 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2521 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     2701 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3181 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3301 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3421 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3601 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     3661 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3721 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3781 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3841 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     3901 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3961 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4021 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4081 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4141 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4201 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4261 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4321 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4381 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4441 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4501 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4561 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4621 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     4681 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     4741 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4801 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4861 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4921 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5701 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5761 gtggataacc gtattaccgc catgcat
//
