LOCUS       Exported                6401 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    637..2319
                     /label=SopB
                     /note="Wild-type"
     misc_feature    2347..3066
                     /label=EGFP
     misc_feature    3946..4214
                     /label=SV40_promoter
     misc_feature    4300..5088
                     /label=NeoR/KanR
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctaatgc aaatacagag cttctatcac
      661 tcagcttcac taaaaaccca ggaggctttt aaaagcctac aaaaaacctt atacaacgga
      721 atgcagattc tctcaggcca gggcaaagcg ccggctaaag cgcccgacgc tcgcccggaa
      781 attattgtcc tgcgagaacc cggcgcgaca tgggggaatt atctacagca tcagaaggcg
      841 tctaaccact cgctgcataa cctctataac ttacagcgcg atcttcttac cgtcgcggca
      901 accgttctgg gtaaacaaga cccggttcta acgtcaatgg caaaccaaat ggagttagcc
      961 aaagttaaag cggaccggcc agcaacaaaa caagaagaag ccgcggcaaa agcattgaag
     1021 aaaaatctta tcgaacttat tgcagcacgc actcagcagc aggatggctt acctgcaaaa
     1081 gaagctcatc gctttgcggc agtagcgttt agagatgctc aggtcaagca gcttaataac
     1141 cagccctggc aaaccataaa aaatacactc acgcataacg ggcatcacta taccaacacg
     1201 cagctccctg cagcagagat gaaaatcggc gcaaaagata tctttcccag tgcttatgag
     1261 ggaaagggcg tatgcagttg ggataccaag aatattcatc acgccaataa tttgtggatg
     1321 tccacggtga gtgtgcatga ggacggtaaa gataaaacgc ttttttgcgg gatacgtcat
     1381 ggcgtgcttt ccccctatca tgaaaaagat ccgcttctgc gtcacgtcgg cgctgaaaac
     1441 aaagccaaag aagtattaac tgcggcactt tttagtaaac ctgagttgct taacaaagcc
     1501 ttagcgggcg aggcggtaag cctgaaactg gtatccgtcg ggttactcac cgcgtcgaat
     1561 attttcggca aagagggaac gatggtcgag gaccaaatgc gcgcatggca atcgttgacc
     1621 cagccgggaa aaatgattca tttaaaaatc cgcaataaag atggcgatct acagacggta
     1681 aaaataaaac cggacgtcgc cgcatttaat gtgggtgtta atgagctggc gctcaagctc
     1741 ggctttggcc ttaaggcatc ggatagctat aatgccgagg cgctacatca gttattaggc
     1801 aatgatttac gccctgaagc cagaccaggt ggctgggttg gcgaatggct ggcgcaatac
     1861 ccggataatt atgaggtcgt caatacatta gcgcgccaga ttaaggatat atggaaaaat
     1921 aaccaacatc ataaagatgg cggcgaaccc tataaactcg cacaacgcct tgccatgtta
     1981 gcccatgaaa ttgacgcggt acccgcctgg aattgtaaaa gcggcaaaga tcgtacaggg
     2041 atgatggatt cagaaatcaa gcgagagatc atttccttac atcagaccca tatgttaagt
     2101 gcgcctggta gtcttccgga tagcggtgga cagaaaattt tccaaaaagt attactgaat
     2161 agcggtaacc tggagattca gaaacaaaat acgggcgggg cgggaaacaa agtaatgaaa
     2221 aatttatcgc cagaggtgct caatctttcc tatcaaaaac gagttgggga tgaaaatatt
     2281 tggcagtcag taaaaggcat ttcttcatta atcacatctc ccgggatcca ccggccggtc
     2341 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2401 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2461 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2521 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2581 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2641 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2701 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2761 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2821 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2881 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2941 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3001 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3061 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     3121 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3181 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3301 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3361 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3421 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3481 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3541 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3601 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3661 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3721 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3901 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4021 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4081 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4141 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4201 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4261 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4321 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4381 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4441 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4501 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4561 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4621 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4681 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4741 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4801 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4861 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4921 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4981 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5041 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5101 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5161 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5221 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     5281 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     5341 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5401 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5461 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     5521 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     5581 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5641 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5821 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6061 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6361 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
