LOCUS       Exported                6303 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    613..1329
                     /label=EGFP
     misc_feature    1330..1392
                     /label=Linker
     misc_feature    1393..2958
                     /label=SopB(33-554)
     misc_feature    2959..2961
                     /label=STOP
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtggt
     1381 agtgcttccg gatcaggcca gggcaaagcg ccggctaaag cgcccgacgc tcgcccggaa
     1441 attattgtcc tgcgagaacc cggcgcgaca tgggggaatt atctacagca tcagaaggcg
     1501 tctaaccact cgctgcataa cctctataac ttacagcgcg atcttcttac cgtcgcggca
     1561 accgttctgg gtaaacaaga cccggttcta acgtcaatgg caaaccaaat ggagttagcc
     1621 aaagttaaag cggaccggcc agcaacaaaa caagaagaag ccgcggcaaa agcattgaag
     1681 aaaaatctta tcgaacttat tgcagcacgc actcagcagc aggatggctt acctgcaaaa
     1741 gaagctcatc gctttgcggc agtagcgttt agagatgctc aggtcaagca gcttaataac
     1801 cagccctggc aaaccataaa aaatacactc acgcataacg ggcatcacta taccaacacg
     1861 cagctccctg cagcagagat gaaaatcggc gcaaaagata tctttcccag tgcttatgag
     1921 ggaaagggcg tatgcagttg ggataccaag aatattcatc acgccaataa tttgtggatg
     1981 tccacggtga gtgtgcatga ggacggtaaa gataaaacgc ttttttgcgg gatacgtcat
     2041 ggcgtgcttt ccccctatca tgaaaaagat ccgcttctgc gtcacgtcgg cgctgaaaac
     2101 aaagccaaag aagtattaac tgcggcactt tttagtaaac ctgagttgct taacaaagcc
     2161 ttagcgggcg aggcggtaag cctgaaactg gtatccgtcg ggttactcac cgcgtcgaat
     2221 attttcggca aagagggaac gatggtcgag gaccaaatgc gcgcatggca atcgttgacc
     2281 cagccgggaa aaatgattca tttaaaaatc cgcaataaag atggcgatct acagacggta
     2341 aaaataaaac cggacgtcgc cgcatttaat gtgggtgtta atgagctggc gctcaagctc
     2401 ggctttggcc ttaaggcatc ggatagctat aatgccgagg cgctacatca gttattaggc
     2461 aatgatttac gccctgaagc cagaccaggt ggctgggttg gcgaatggct ggcgcaatac
     2521 ccggataatt atgaggtcgt caatacatta gcgcgccaga ttaaggatat atggaaaaat
     2581 aaccaacatc ataaagatgg cggcgaaccc tataaactcg cacaacgcct tgccatgtta
     2641 gcccatgaaa ttgacgcggt acccgcctgg aattgtaaaa gcggcaaaga tcgtacaggg
     2701 atgatggatt cagaaatcaa gcgagagatc atttccttac atcagaccca tatgttaagt
     2761 gcgcctggta gtcttccgga tagcggtgga cagaaaattt tccaaaaagt attactgaat
     2821 agcggtaacc tggagattca gaaacaaaat acgggcgggg cgggaaacaa agtaatgaaa
     2881 aatttatcgc cagaggtgct caatctttcc tatcaaaaac gagttgggga tgaaaatatt
     2941 tggcagtcag taaaaggcta aggatccacc ggatctagat aactgatcat aatcagccat
     3001 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3061 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     3121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3181 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     3241 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3301 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3361 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3421 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3481 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3541 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3601 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3661 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3781 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4141 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     4201 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4261 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4321 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4381 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4441 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4501 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4561 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4621 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4681 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4741 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4801 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4861 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4921 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4981 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5041 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5101 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5161 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     5221 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     5281 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5341 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5401 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     6301 cat
//
