LOCUS       Exported                6351 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    613..1329
                     /label=EGFP
     misc_feature    1393..3009
                     /label=IpgD
     misc_feature    2526
                     /label=Silent bp mutation
     misc_feature    3007..3009
                     /label=STOP
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtggt
     1381 agtgcttccg gaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga
     1441 gattcctata aaggcgcaga agaaaccgga aagcacaaag gtgtaagcgt gatttcatat
     1501 caaagagtta aaaatggaga aagaaataag gggatcgaag cgcttaatcg attatattta
     1561 cagaaccaaa caagcttaac aggaaaaagc cttttgtttg cgcgtgacaa agctgaagtt
     1621 ttttgcgagg caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa
     1681 cgattagata cctataaact tggtgaagtt aataaaagac atattaatga gcttaataaa
     1741 gtaataagtg aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaaacg
     1801 aaaataaaac aaatatttac tgattattta aataataaga attggggacc ggtcaacaag
     1861 aatattagtc atcatgggaa aaattatagt tttcaattaa ctcctgcctc tcatatgaag
     1921 atagggaata aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct
     1981 acgagagagc gtgatcatat cgcgaatatg tggctttcaa aagtggtgga tgacgagggg
     2041 aaggaaattt tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac
     2101 tcatctgaaa gagctgttgc tgctcgtaat aaagctgagg agttagtaag cgcagcatta
     2161 tatagcagac cagagttatt atcacaggct ttgtctggta aaacagtaga tttaaagatt
     2221 gtttcgactt ctcttctgac gccgaccagt ttaaccgggg gagaggagag tatgttaaaa
     2281 gatcaggtga gcgcattaaa agggttaaac tctaaaaggg gggggccaac gaaattatta
     2341 attcggaata gtgatggtct tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt
     2401 aactttggtg taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag
     2461 cttaatgatg aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt
     2521 ggaggctggg ctgcagaagc aatagaaaaa aatccaccat gtaagaatga tgtgatatat
     2581 ctggctaacc agataaaaga gattgtaaac aataagttac aaaaaaatga taatggagag
     2641 ccgtataaat tgtcacaaag agtgactcta ttggcttata ctataggtgc tgtaccttgc
     2701 tggaattgta agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa
     2761 ataataagaa aacatgaaac aggtcagttt tctcaattaa atagtaaatt atcctcagaa
     2821 gagaaaagat tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg
     2881 aatactggtg tgcccggaaa taaagtcatg aaaaaattgc cgctatcttc gttagagcta
     2941 tcttattctg aaagaatagg ggacccaaaa atatggaata tggtgaaagg gtattcgtca
     3001 tttgtataag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     3061 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3121 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3241 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3481 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3721 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3841 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3901 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3961 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4021 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4081 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4141 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4201 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4261 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4321 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4381 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4441 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4501 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4561 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4621 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4681 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4741 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4801 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4861 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4921 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4981 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5041 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5101 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5161 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5221 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5281 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5341 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5401 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5461 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5521 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5581 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5641 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5701 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5761 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5821 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5881 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5941 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6001 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6061 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
