LOCUS       Exported                9300 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    546..571
                     /label=Primer Annealing (ORF2 rev)
     misc_feature    663..1757
                     /label=LEU2 (variant)
     misc_feature    3610..4060
                     /label=GAL1 promoter
     misc_feature    4067..5680
                     /label=IpgD (Shigella flexneri)
     misc_feature    5381..5383
                     /label=Catalytic Cysteine [*serine inactivation]
     misc_feature    5681..5683
                     /label=STOP
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctgaatt ggagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
     3061 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
     3121 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
     3181 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat
     3241 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa
     3301 gaattgttaa ttaagagctc agatcttatc gtcgtcatcc ttgtaatcca tcgatactag
     3361 tgcggccgcc ctttagtgag ggttgaattc gaattttcaa aaattcttac tttttttttg
     3421 gatggacgca aagaagttta ataatcatat tacatggcat taccaccata tacatatcca
     3481 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc
     3541 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta
     3601 tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc
     3661 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca
     3721 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt
     3781 ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg
     3841 ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt
     3901 ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc
     3961 aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg
     4021 ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatatgc acataactaa
     4081 tttgggattg catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac
     4141 cggaaagcac aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa
     4201 taaggggatc gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa
     4261 aagccttttg tttgcgcgtg acaaagctga agttttttgc gaggcaataa agttggcagg
     4321 tggtgatacg tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga
     4381 agttaataaa agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca
     4441 gctaggcatt aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta
     4501 tttaaataat aagaattggg gaccggtcaa caagaatatt agtcatcatg ggaaaaatta
     4561 tagttttcaa ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa
     4621 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagcgtgatc atatcgcgaa
     4681 tatgtggctt tcaaaagtgg tggatgacga ggggaaggaa attttttcgg gtatcagaca
     4741 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg
     4801 taataaagct gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca
     4861 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
     4921 cagtttaacc gggggagagg agagtatgtt aaaagatcag gtgagcgcat taaaagggtt
     4981 aaactctaaa aggggggggc caacgaaatt attaattcgg aatagtgatg gtcttctgaa
     5041 agaagtaagc gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
     5101 aaaaatgggc ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt
     5161 gttgggggat aatttcctca aaaatggtgt gattggaggc tgggctgcag aagcaataga
     5221 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt
     5281 aaacaataag ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagagtgac
     5341 tctattggct tatactatag gtgctgtacc ttgctggaat tcgaagagtg ggaaggacag
     5401 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca
     5461 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
     5521 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt
     5581 catgaaaaaa ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc
     5641 aaaaatatgg aatatggtga aagggtattc gtcatttgta taagcttggt accgcggcta
     5701 gctaagatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     5761 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     5821 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     5881 tttgggacgc tcgaagatcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6541 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7741 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7861 gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac
     7921 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     7981 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
     8041 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     8101 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
     8161 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
     8221 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
     8281 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
     8341 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
     8401 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
     8461 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
     8521 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
     8581 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
     8641 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
     8701 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
     8761 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
     8821 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg
     8881 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
     8941 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
     9001 tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc
     9061 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
     9121 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
     9181 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag
     9241 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
