LOCUS       Exported                6847 bp DNA     circular SYN 07-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
COMMENT     12-August-17
            Edward Avezov
            439684901_4UK1735haloK73T_pCMVF_E10.ab1
FEATURES             Location/Qualifiers
     source          1..6847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..158
                     /label=lac operon sequence
                     /note="lac operon sequence"
     primer_bind     114..137
                     /label=pUC/M13 forward sequencing primer
                     /note="pUC/M13 forward sequencing primer"
     primer_bind     141..156
                     /label=M13 forward
                     /note="M13 forward"
     misc_feature    398..685
                     /label=CAG_enhancer""
     primer_bind     809..828
                     /label=543 CMVF_pCDNA3 (SB)
                     /note="543 CMVF_pCDNA3 (SB)"
     misc_feature    852..872
                     /label=CMV_fwd_primer""
     primer_bind     852..872
                     /label=97
                     /note="97"
     misc_feature    896..915
                     /label=pCEP_fwd_primer
                     /note="/translation=""MGGRRVRWEVYISRARLVNRQIAWRRHPRCFDLHRRH
                     RDRSSLR 
                     TLEDRTLATMDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG 
                     ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLV 
                     EEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR 
                     GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE 
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ 
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALV 
                     RQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNRED 
                     LLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL 
                     ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSL 
                     LYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKK 
                     IECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDR 
                     EMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDG 
                     FANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD 
                     ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVEN 
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSD 
                     KNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKR 
                     QLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE 
                     INNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAK 
                     YFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVN 
                     IVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK 
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG 
                     RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD 
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFK 
                     YFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV*"""
     primer_bind     896..915
                     /label=pCEP-Forward (SB)
                     /note="pCEP-Forward (SB)"
     primer_bind     897..917
                     /label=512 pCMV forward (SB)
                     /note="512 pCMV forward (SB)"
     primer_bind     903..934
                     /label=428
                     /note="428"
     CDS             932..1930
                     /codon_start=1
                     /label=H6-Halo-KDEL
                     /note="H6-Halo-KDEL"
                     /translation="MSALLILALVGAAVAMHHHHHHAAASLEAEIGTGFPFDPHYVEVL
                     GERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDTPDL
                     GYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIP
                     TWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLN
                     PVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAAR
                     LAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTVDGSADLAAKDEL"
     misc_feature    932..976
                     /label=leader seq mouse preprotrypsin
     primer_bind     934..954
                     /label=151
                     /note="151"
     misc_feature    join(976..1924,1927..1955)
                     /label=confirmed by sequencing2
                     /note=" [Split]"
     misc_feature    977..1912
                     /label=Halo
     primer_bind     977..994
                     /label=Mito_H6-HaloTag-KDEL_Gibson_1.S
                     /note="Mito_H6-HaloTag-KDEL_Gibson_1.S"
     primer_bind     complement(1078..1095)
                     /label=570
                     /note="570"
     misc_feature    1232..1234
                     /label=K73T AAA->ACA
                     /note="K73T AAA->ACA"
     primer_bind     complement(1913..1930)
                     /label=Mito_H6-HaloTag-KDEL_Gibson_2.AS
                     /note="Mito_H6-HaloTag-KDEL_Gibson_2.AS"
     misc_feature    1914..1919
                     /label=Nco
     misc_feature    join(1924,1927..3123)
                     /label=DEL CALR protein coding 1
                     /note=" [Split]"
     misc_feature    2204..3113
                     /label=confirmed by sequencing
     primer_bind     complement(3196..3215)
                     /label=pCMV reverse (SB)
                     /note="pCMV reverse (SB)"
     primer_bind     complement(3215..3239)
                     /label=164
                     /note="164"
     primer_bind     complement(3215..3234)
                     /label=440
                     /note="440"
     enhancer        3812..4048
                     /label=SV40 Enhancer
                     /note="SV40"
     misc_feature    3817..3837
                     /label=pBABE_3_primer
                     /note="/translation=MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQV
                     DNVFC 
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR 
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD 
                     LFISFRLEKVDGVFFEA*"""
     misc_feature    3823..4038
                     /label=SV40_enhancer
                     /note="/translation=MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQV
                     DNVFC 
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR 
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD 
                     LFISFRLEKVDGVFFEA*"""
     rep_origin      4002..4079
                     /label=SV40
                     /label=SV40_origin
                     /note="/translation=MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQV
                     DNVFC 
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR 
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD 
                     LFISFRLEKVDGVFFEA*"""
     rep_origin      4051..4126
                     /direction=BOTH
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4064..4083
                     /label=SV40pro_F_primer
                     /note="/translation=MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQV
                     DNVFC 
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR 
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD 
                     LFISFRLEKVDGVFFEA*"""
     misc_feature    4077..4106
                     /label=SV40 promoter directed transcriptional start site
                     /note="SV40 promoter directed transcriptional start site"
     misc_feature    4083..4112
                     /label=SV40 promoter directed transcriptional start site
                     /note="SV40 promoter directed transcriptional start site"
     misc_feature    4088..4117
                     /label=SV40 promoter directed transcriptional start site
                     /note="SV40 promoter directed transcriptional start site"
     promoter        complement(4189..4208)
                     /note="T7"
     primer_bind     complement(4189..4208)
                     /label=T7 universal primer sequence (NEB #S1248S)
                     /note="T7 universal primer sequence (NEB #S1248S)"
     misc_feature    4213..4459
                     /label=lac operon sequence
                     /note="lac operon sequence"
     misc_feature    4213..4441
                     /label=Lac-operon
                     /note="Lac-operon"
     primer_bind     complement(4222..4243)
                     /label=pUC/M13 reverse sequencing primer
                     /note="pUC/M13 reverse sequencing primer"
     primer_bind     4222..4238
                     /label=binding site of pUC/M13 reverse sequencing primer
                     /note="binding site of pUC/M13 reverse sequencing primer"
     promoter        complement(4227..4348)
                     /note="Plac"
     primer_bind     complement(4235..4258)
                     /note="Ptac forward sequencing primer (NEB #1260, 
                     discontinued)"
     protein_bind    4242..4266
                     /label=LacI binding site
                     /bound_moiety="LacI"
                     /note="lac operator"
     protein_bind    4320..4332
                     /label=catabolite activator protein (CAP) binding site
                     /bound_moiety="catabolite activator protein (CAP)"
                     /note="CAP protein binding site"
     misc_feature    4441..4504
                     /label=L-strand Y effector site
                     /note="L-strand Y effector site"
                     /citation=[5]
     rep_origin      4564..5448
                     /label=pUC Origin
                     /note="pUC Origin"
     misc_feature    4565..5238
                     /label=pUC origin
                     /note="pUC origin"
     rep_origin      4565..5238
                     /label=pUC
                     /note="pUC"
     rep_origin      4621..4657
                     /label=ori
                     /note="ColE1-derived origin of replication"
     rep_origin      4624..5212
                     /note="pMB1 origin of replication (counterclockwise) (RNAII
                     -35 to RNA/DNA switch point)"
     misc_RNA        complement(4624..5176)
                     /label=RNAII transcript (complementary strand)
                     /note="RNAII transcript (complementary strand)"
     misc_RNA        5066..5173
                     /label=RNAI transcript
                     /note="RNAI transcript"
     repeat_region   5238..5275
                     /note="corresponds to one of the 38bp repeats found in Tn3 
                     (bp 1-38 and complement (4920-4957))"
     CDS             complement(5383..6243)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="E-286"
                     /db_xref="GI:455370"
                     /protein_id="AAB59737.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5383..6243)
                     /gene="bla"
                     /label=bla
     misc_feature    5386..6237
                     /label=""beta-lactamase TEM
                     /note="""beta-lactamase TEM"
                     /note="Provisional"
                     /note="Region: PRK15442"""
                     /db_xref="CDD:185339"
     mat_peptide     5386..6174
                     /gene="bla"
                     /product="beta-lactamase"
                     /note=" [Split]"
     sig_peptide     6175..6243
                     /gene="bla"
                     /note="Required for secretion to the periplasm"
                     /note="cleaved off to form the mature beta-lactamase 
                     protein."
     promoter        complement(6285..6313)
                     /label=pAmpR
                     /label=AmpR_promoter
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"""
     rep_origin      6375..6830
                     /note="bacteriophage f1 origin of replication for 
                     production of single-stranded DNA"
     misc_feature    6375..6830
                     /label=phage f1 region
                     /note="phage f1 region"
     misc_feature    6375..6830
                     /label=phage f1 region
                     /note="phage f1 region"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctatgc accatcatca tcaccatgcg gccgcatctc tcgaggcaga
     1021 aatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca
     1081 ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac
     1141 ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc
     1201 tccagacctg atcggtatgg gcaaatccga cacaccagac ctgggttatt tcttcgacga
     1261 ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt
     1321 cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt
     1381 caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga
     1441 atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat
     1501 cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga
     1561 agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg
     1621 gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga
     1681 agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc
     1741 aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa
     1801 ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg
     1861 cagcgagatc gcgcgctggc tttcgacggt cgacggatcc gcagatctag cggccaagga
     1921 tgagttgtag tccgtgccgc tgctgctcgg cctcctcggc ctggccgtcg ccgagcctgc
     1981 cgtctacttc aaggagcagt ttctggacgg agacgggtgg acttcccgct ggatcgaatc
     2041 caaacacaag tcagattttg gcaaattcgt tctcagttcc ggcaagttct acggtgacga
     2101 ggagaaagat aaaggtttgc agacaagcca ggatgcacgc ttttatgctc tgtcggccag
     2161 tttcgagcct ttcagcaaca aaggccagac gctggtggtg cagttcacgg tgaaacatga
     2221 gcagaacatc gactgtgggg gcggctatgt gaagctgttt cctaatagtt tggaccagac
     2281 agacatgcac ggagactcag aatacaacat catgtttggt cccgacatct gtggccctgg
     2341 caccaagaag gttcatgtca tcttcaacta caagggcaag aacgtgctga tcaacaagga
     2401 catccgttgc aaggatgatg agtttacaca cctgtacaca ctgattgtgc ggccagacaa
     2461 cacctatgag gtgaagattg acaacagcca ggtggagtcc ggctccttgg aagacgattg
     2521 ggacttcctg ccacccaaga agataaagga tcctgatgct tcaaaaccgg aagactggga
     2581 tgagcgggcc aagatcgatg atcccacaga ctccaagcct gaggactggg acaagcccga
     2641 gcatatccct gaccctgatg ctaagaagcc cgaggactgg gatgaagaga tggacggaga
     2701 gtgggaaccc ccagtgattc agaaccctga gtacaagggt gagtggaagc cccggcagat
     2761 cgacaaccca gattacaagg gcacttggat ccacccagaa attgacaacc ccgagtattc
     2821 tcccgatccc agtatctatg cctatgataa ctttggcgtg ctgggcctgg acctctggca
     2881 ggtcaagtct ggcaccatct ttgacaactt cctcatcacc aacgatgagg catacgctga
     2941 ggagtttggc aacgagacgt ggggcgtaac aaaggcagca gagaaacaaa tgaaggacaa
     3001 acaggacgag gagcagagga caaggaggat gatgaggaca aagatgagga tgaggaggat
     3061 gaggaggaca aggaggaaga tgaggaggaa gatgtccccg gccaggccaa ggacgagctg
     3121 tagagaggcc tgcctccagg gctggactga ggcctgaact agaggatccc gggtggcatc
     3181 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     3241 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     3301 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     3361 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     3421 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3481 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3541 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3601 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
     3661 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg
     3721 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
     3781 tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg
     3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3901 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4141 gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc ctatagtgag
     4201 tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4261 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4321 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4381 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5041 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5341 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5401 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5461 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5521 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5581 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5641 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5701 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5761 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5821 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5881 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5941 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6001 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6061 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6361 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6781 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccatacaatt
     6841 tcaattt
//
