LOCUS       Exported                9709 bp DNA     circular SYN 13-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 8, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 132 .. 200 / #ccffcc / signal sequence
                        2: 201 .. 992 / #ccffcc
                     Cleavage site after base 200"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2075 .. 2080 / #ffffff / -35
                        2: 2081 .. 2098 / #ffffff
                        3: 2099 .. 2105 / #ffffff / -10"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    4465..4582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        4639..5876
                     /label=UBC
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      5883..5888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             5889..7319
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     7279..7340
                     /label=3U_F
                     /note="color: black; sequence: 
                     ACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGTAGgcggactgttactgagct
                     gcg; added: 2020-05-07"
     primer_bind     7279..7319
                     /label=cloning overhang F
                     /note="color: black; sequence: 
                     ACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGTAG; added: 
                     2020-05-06"
     3'UTR           7320..7759
                     /note="color: #ff6600"
     primer_bind     7320..7340
                     /label=Fwd after stop
                     /note="color: black; sequence: gcggactgttactgagctgcg; 
                     added: 2019-12-19"
     misc_feature    7519..7538
                     /label=sgRNA 222fw
                     /note="color: #a6acb3"
     misc_feature    7555..7574
                     /label=sgRNA 258fw
                     /note="color: #a6acb3"
     misc_feature    7559..7578
                     /label=sgRNA 239rev
                     /note="color: #a6acb3"
     primer_bind     7660..7679
                     /label=3UTR1 chkF
                     /note="color: black; sequence: aagtggttacaggaagtccc; added:
                     2020-05-13"
     primer_bind     7722..7739
                     /label=3' UTR check F
                     /note="color: black; sequence: tcgcgtccatgccctgag; added: 
                     2020-02-11"
     primer_bind     complement(7741..7802)
                     /label=3U_R
                     /note="color: black; sequence: 
                     CACAAATTTTGTAATCCAGAGGTTGATTGTCGACGCGGCCGCTgtcaccttccccgggg
                     tgg; added: 2020-05-07"
     primer_bind     complement(7741..7759)
                     /label=reverse
                     /note="color: black; sequence: gtcaccttccccggggtgg; added: 
                     2020-05-07"
     primer_bind     7741..7759
                     /label=MBS-F
                     /note="MBS-R is inside the MBS repeat"
                     /note="color: black; sequence: ccaccccggggaaggtgac; added: 
                     2020-01-14"
     primer_bind     complement(7743..7759)
                     /label=Rev from MBS insertion
                     /note="color: black; sequence: gtcaccttccccggggt; added: 
                     2019-12-19"
     primer_bind     complement(7760..7802)
                     /label=UTR overhang
                     /note="color: black; sequence: 
                     CACAAATTTTGTAATCCAGAGGTTGATTGTCGACGCGGCCGCT; added: 
                     2019-04-30"
     primer_bind     complement(7774..7799)
                     /label=cloning overhang R
                     /note="color: black; sequence: AAATTTTGTAATCCAGAGGTTGATTG; 
                     added: 2020-05-06"
     misc_feature    7775..8363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(7882..7901)
                     /label=WPRE-R
                     /note="color: black; sequence: AAGCCATACGGGAAGCAATA; added:
                     2020-02-10"
     primer_bind     8163..8175
                     /label=MBS-F
                     /note="MBS-R is inside the MBS repeat"
                     /note="color: black; sequence: ccaccccggggaaggtgac; added: 
                     2020-01-14"
     CDS             complement(8246..8257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 8245"
                     /translation="IEGR"
     LTR             8450..8683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8755..8876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      8895..9030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(9063..9081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(9091..9107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      9252..9707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     3121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     3181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     3241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     3301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     3361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     3421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     3481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     3541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     3601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     3661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     3721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     3781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     4021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     4081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     4141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     4201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     4261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     4321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     4381 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     4441 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     4501 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4561 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgattaacc
     4621 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     4681 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     4741 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     4801 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     4861 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     4921 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     4981 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     5041 gttgcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     5101 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     5161 tgggggttgg ggggagcgca caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     5221 tgtaaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     5281 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     5341 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg gtttgtcgtc
     5401 tggttgcggg ggcggcagtt atgcggtgcc gttgggcagt gcacccgtac ctttgggagc
     5461 gcgcgcctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     5521 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     5581 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     5641 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     5701 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     5761 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     5821 tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga gatccaccgg
     5881 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     5941 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6001 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     6061 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     6121 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6181 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6241 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6301 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6361 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6421 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6481 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6541 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     6601 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     6661 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     6721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     6781 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     6841 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     6901 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     6961 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     7021 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     7081 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     7141 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     7201 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7261 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtagg
     7321 cggactgtta ctgagctgcg ttttacaccc tttctttgac aaaacctaac ttgcgcagaa
     7381 aaaaaaaaaa taagagacaa cattggcatg gctttgtttt tttaaatttt ttttaaagtt
     7441 tttttttttt tttttttttt tttttttaag tttttttgtt ttgttttggc gcttttgact
     7501 caggatttaa aaactggaac ggtgaaggcg acagcagttg gttggagcaa acatccccca
     7561 aagttctaca aatgtggctg aggactttgt acattgtttt gttttttttt ttttttggtt
     7621 ttgtcttttt ttaatagtca ttccaagtat ccatgaaata agtggttaca ggaagtccct
     7681 caccctccca aaagccaccc ccactcctaa gaggaggatg gtcgcgtcca tgccctgagt
     7741 ccaccccggg gaaggtgaca gcggccgcgt cgacaatcaa cctctggatt acaaaatttg
     7801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8161 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     8221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8281 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8341 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact
     8401 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8461 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     8521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     8701 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     8761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8881 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8941 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9001 agaagtagtg aggaggcttt tttggaggcc taggcttttg cgtcgagacg tacccaattc
     9061 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9241 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9301 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9361 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9421 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9481 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9541 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9601 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9661 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcc
//
