LOCUS       Exported                9505 bp DNA     circular SYN 13-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 8, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 132 .. 200 / #ccffcc / signal sequence
                        2: 201 .. 992 / #ccffcc
                     Cleavage site after base 200"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2075 .. 2080 / #ffffff / -35
                        2: 2081 .. 2098 / #ffffff
                        3: 2099 .. 2105 / #ffffff / -10"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    4465..4582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        4639..5876
                     /label=UBC
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      5883..5888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             5889..7319
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     7279..7345
                     /label=3U1_F
                     /note="color: black; sequence: 
                     ACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGTAGcattgcttctgtgtaaat
                     tatgtact; added: 2020-05-07"
     primer_bind     7279..7319
                     /label=cloning overhang F
                     /note="color: black; sequence: 
                     ACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGTAG; added: 
                     2020-05-06"
     3'UTR           7320..7555
                     /label=3' UTR 2
                     /note="color: #ff6600"
     primer_bind     complement(7320..7347)
                     /label=MBS-R
                     /note="color: black; sequence: 
                     gcagtacataatttacacagaagcaatg; added: 2020-01-16"
     primer_bind     7320..7345
                     /label=Fwf after MBS
                     /note="color: black; sequence: cattgcttctgtgtaaattatgtact; 
                     added: 2019-12-18"
     misc_feature    7390..7409
                     /label=sgRNA 535 fw
                     /note="color: #a6acb3"
     misc_feature    7411..7430
                     /label=sgRNA 533rev
                     /note="color: #a6acb3"
     primer_bind     7450..7469
                     /label=3UTR2 chkF
                     /note="color: black; sequence: gtatgaaggctttggtctcc; added:
                     2020-05-13"
     misc_feature    7455..7474
                     /label=sgRNA600fw
                     /note="color: #a6acb3"
     misc_feature    7471..7490
                     /label=sgRNA 593rev
                     /note="color: #a6acb3"
     primer_bind     complement(7483..7508)
                     /label=Actb_MBS Rev
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     7506..7524
                     /label=2-3'UTR check F
                     /note="color: black; sequence: ggcctgtacactgacttga; added: 
                     2020-02-10"
     polyA_signal    7530..7534
                     /label=Feature 3
                     /note="color: #a6acb3"
     primer_bind     complement(7533..7555)
                     /label=Rev from end
                     /note="color: black; sequence: gtgtaaggtaaggtgtgcacttt; 
                     added: 2019-12-18"
     primer_bind     complement(7536..7598)
                     /label=3U2_R
                     /note="color: black; sequence: 
                     CACAAATTTTGTAATCCAGAGGTTGATTGTCGACGCGGCCGCTgtgtaaggtaaggtgt
                     gcac; added: 2020-05-07"
     primer_bind     complement(7536..7555)
                     /label=reverse
                     /note="color: black; sequence: gtgtaaggtaaggtgtgcac; added:
                     2020-05-07"
     primer_bind     complement(7556..7598)
                     /label=UTR overhang
                     /note="color: black; sequence: 
                     CACAAATTTTGTAATCCAGAGGTTGATTGTCGACGCGGCCGCT; added: 
                     2019-04-30"
     primer_bind     complement(7570..7595)
                     /label=cloning overhang R
                     /note="color: black; sequence: AAATTTTGTAATCCAGAGGTTGATTG; 
                     added: 2020-05-06"
     misc_feature    7571..8159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(7678..7697)
                     /label=WPRE-R
                     /note="color: black; sequence: AAGCCATACGGGAAGCAATA; added:
                     2020-02-10"
     primer_bind     7959..7971
                     /label=MBS-F
                     /note="MBS-R is inside the MBS repeat"
                     /note="color: black; sequence: ccaccccggggaaggtgac; added: 
                     2020-01-14"
     CDS             complement(8042..8053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 8041"
                     /translation="IEGR"
     LTR             8246..8479
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8551..8672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      8691..8826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(8859..8877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8887..8903)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      9048..9503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     3121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     3181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     3241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     3301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     3361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     3421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     3481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     3541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     3601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     3661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     3721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     3781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     4021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     4081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     4141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     4201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     4261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     4321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     4381 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     4441 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     4501 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4561 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgattaacc
     4621 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     4681 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     4741 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     4801 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     4861 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     4921 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     4981 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     5041 gttgcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     5101 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     5161 tgggggttgg ggggagcgca caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     5221 tgtaaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     5281 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     5341 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg gtttgtcgtc
     5401 tggttgcggg ggcggcagtt atgcggtgcc gttgggcagt gcacccgtac ctttgggagc
     5461 gcgcgcctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     5521 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     5581 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     5641 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     5701 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     5761 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     5821 tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga gatccaccgg
     5881 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     5941 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6001 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     6061 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     6121 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6181 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6241 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6301 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6361 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6421 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6481 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6541 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     6601 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     6661 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     6721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     6781 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     6841 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     6901 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     6961 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     7021 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     7081 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     7141 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     7201 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7261 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtagc
     7321 attgcttctg tgtaaattat gtactgcaaa aattttttta aatcttccgc cttaatactt
     7381 catttttgtt tttaatttct gaatggccca ggtctgaggc ctcccttttt tttgtccccc
     7441 caacttgatg tatgaaggct ttggtctccc tgggaggggg ttgaggtgtt gaggcagcca
     7501 gggctggcct gtacactgac ttgagaccaa taaaagtgca caccttacct tacacagcgg
     7561 ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7621 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7681 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7741 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7801 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7861 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7921 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7981 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8041 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8101 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     8161 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8221 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8281 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8341 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8401 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8461 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8521 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8761 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8821 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc
     8881 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8941 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     9001 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
     9061 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9121 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9181 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9241 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9301 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9361 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9421 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9481 caaaatatta acgtttacaa tttcc
//
