LOCUS       Exported               12576 bp ds-DNA     circular SYN 09-MAR-2022
DEFINITION  Ki67_All rotated to position 5807.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific & Educational 
            Software
            SECNOTES|Vector molecule:  KI67_long_mNeonGreen_IRESpuro2*
               Fragment ends: BamHI and BstBI
               Fragment size: 5804
            Insert molecule:  Ki67_All_Repeats*
               Fragment ends: BstBI and BamHI
               Fragment size: 6772
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..502
                     /gene="CMV'"
                     /label=CMV'
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     CDS             572..7338
                     /codon_start=1
                     /gene="MKI67"
                     /label=MKI67
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /translation="M*LKCPASHYNQNQ*TPQHTQNNS*RHPWGK*V*KKSS*QSASSH
                     GRQGRPRTRTESQQEMARASERLRSLQSRSWTQQPV*LE*RSGQERLRKRPSH*KTWLA
                     SKSSSRHQVPLRNQ*LMRKLPK*PANLHHQNQWTLQQAQSNGLREVSGKQM*RKNS*HS
                     GN*HHQQGKPCLRPNQQEVMRKTLKHLWELQCRNWTWQELYLAAKDSYRLLRKRPRL*K
                     TWLALKSSSRLLVTPRN*WLLVKPLKYPATLHSQTQWTPQQAQSNDPREVSGKQM*REN
                     S*RAGI*CHQQAKPCTRLNHQ*VKRKTSSYLWELQCRNWT*QRT*PAARDGHKLLRKRP
                     RLWKT*LALKSSSRPLVILKKQWLLAKLLKCPANLLHQNQQTPQQAQEGSPRHLWRKGT
                     YRRSSQP*RSSHRHQGKPHTQIKYQEVRIKASTRLGKLQNRNWTQQQV*LVARGTQKLR
                     KRPNP*KTWLA*KSSSRHQYALTSPRLTRKLPK*PADHNQTQWTHQQAPSHSPREVSGK
                     WT*KKNSSHSGNEHHQQAKPCTHPNQQ*VVRKTSTHLWELQCRNWT*QRT*LAARDGYK
                     LLRKRPRL*KTWLALKSSSRHEVTLRNQ*LMIKLPK*PANLHNQTQTKTQQAPSDGSRH
                     PWGKWA*KKSS*QLASSHRHQERLHTHTQSQQEMVRA*KHLWSLQSRS*TQQQV*LAAR
                     GS*ELLRESLKSLKTWPASSSSSRHQVTLRNQ*LMKKLPKYPTELHSQT*WTPQQAPSH
                     SPREVSGKQTLKKNF*HLGNKRHQQAKPCTHPNQQ*VKRKTSTRFWELQCRNWTSQEIY
                     LAAIDGYKLVRKRPRL*KN*LASESFSRHHALITPRLMRKLPKKYSANLRNQTQRTPQQ
                     TQSNGPREASRKQT*RKNF*HSGN*HHQQAKPCTRLKQQ*VKRKTSTHLWGLQWRNWTC
                     *EIYLAARDGHKLLKKRPRL*KIWLASKSSSRHQVTLRNQ*PMTKSQKYPANLHNQTQS
                     KPQQAPSNDSRYPWGK*V*KKRSYQSASSHRRQGRPHRHTERQQEMERASKRLRNLQSR
                     CWTQQTMELGWRGGQEHLRKRPNH*KTWPASKSSSRHQTTLRNQQLMTKLPK*PANLHH
                     QNQWTLQQAQGGGPKHLWGKGI*WKSSQP*SSSHRPHTQTKYQEMRIKASTCSGKLQNR
                     NWTQQQV*LVARGSQELLREKPNP*KTWLA*KSSSRHQYALTSPRLMRKLPK*PADLHN
                     QTQWVPQQSSSHSPREVSGKQT*RKNP*HSGNEHHQ*GKLWTHPNQQEVMRKT*KHLWE
                     LQCRNWTCQEIYLAAKDGHKLLRKRPRL*KTWLASKSSSRHQALTSPRLMRKLPK*PAN
                     LHNQTQWTPQQAQSNGPRETSGKQT*RKNF*HSGNEHHQQAKPWTHQNQQ*VMRKISTH
                     LWKLQCRNWTC*EIYLAARDSHRLLRKRLRL*RTWLASKNSSRHQVTLRNQ*LMTKSQK
                     YPVNLHSQSHSKPQEAPSKGSRYPW*KWT*KKSP*QSASSHGHQGRLRKHTQSQQEIVR
                     ASKRLRSLQSRSWTQQQV*LVAGGS*ELVRKRPVL*KTWLTSKSSSQHQVTLKSQ*LLT
                     KTQKFPANLPHQN*QTLPRAQRDAPRHVPGKK*KRSSQQLRGSRKHQGKAHTHTKNQQA
                     VMRASKY*SNVQRRNQTQ*KRNPAGEGQEHLRKRPNPWKTWPASQSSLKHQVTLRNH*L
                     LAKPLKYPANLPH*KW*TPQQAQRGISGHVCRRYK*KKSLQQSSSHKHQGKPRMQTKNQ
                     QVKIKASKH*RNLQNRHRLQQQV*LAAGDGQEHPGKVPKP*KT*LASKTQQQVTLKNQ*
                     LMTKPLKYPANHHQN*KTPQQAQRDGPGHVPRK*K*RRSC*QLASSHKPQGRPRTPTKS
                     R*VRAKARKHLSNLQSGSWTQKM*LAAGDSQEHLRKRPNPWKIWPASKSSLKHQATLRN
                     WQMVLLIALQALQSKHLTVENL*KYPEEFFGPLK*NPWETW*APETL*NHKAKATLPCP
                     HCPSRGEVAKMEASREPRGCAACQHQRKLWRSCQPARSRGLLPGQEANHPNPWSS*REV
                     *GLLQKELNLRKS*TATT*KPTKRNTNYKTRSLKIREYPCAPDAKIRLRQNSK*LRSLY
                     *QKE*K*TEMKRSP*RPPQRWTFRIQMMEPGNPYLETKSLRTKGA*GLLDRMRAPSLRW
                     QRRAEGRRVRRFSCRIRKGKEKQEIQTPCA*DQERQKASLQQALWRANLCRE*RGVSRG
                     VQKIQRRLRTMCVSRK*EPEVIGTVKI"
     CDS             574..8058
                     /codon_start=1
                     /gene="Ki67_All_Repeats"
                     /label=Ki67_All_Repeats
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=Ki67_All_Repeats"
                     /note="/SECStyleId=1"
                     /translation="MTKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFTR
                     TSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAGF
                     KELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLALR
                     KLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALED
                     LAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGEL
                     LACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEAQ
                     ALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRDV
                     QKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTKE
                     KAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRKV
                     DVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQT
                     PKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTS
                     LGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSKR
                     QLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKPQ
                     PKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNLP
                     GSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPTN
                     TKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDLL
                     GNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPVK
                     TPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQM
                     LDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPPP
                     ESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQK
                     LDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQP
                     DPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGT
                     PVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKS
                     PQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTF
                     VETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEV
                     SCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKS
                     IKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDK
                     NTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASG
                     DEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTA
                     GKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPA
                     GEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMT
                     DDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEP
                     VGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEEL
                     ANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPP
                     LPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSL
                     RTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVL
                     AERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVA
                     EESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRC
                     AENPKKAEDNVCVKKIRTRSHRDSEDIWDPMVSKGEEDNMASLPATHELHIFGSINGVD
                     FDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAM
                     VDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAAD
                     WCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRK
                     TELKHSKTELNFKEWQKAFTDVMGMDELYK"
     misc_signal     2757
                     /label=silent mutation 1
                     /note="/SECDrawAs=Label"
                     /note="/product=C -> T mutation"
     misc_signal     6604
                     /label=silent mutation 2
                     /note="/SECDrawAs=Label"
                     /note="/product=C -> T mutation"
     CDS             7342..8058
                     /codon_start=1
                     /gene="MKI67-mNeonGreen"
                     /label=MKI67-mNeonGreen
                     /note="/SECDrawAs=Gene"
                     /note="/SECName='MKI67-mNeonGreen"
                     /note="/SECStyleId=1"
                     /translation="DPMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDG
                     YEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQF
                     EDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTI
                     ISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKE
                     WQKAFTDVMGMDELYK"
     misc_feature    8091..8386
                     /gene="IVS"
                     /label=IVS
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    8412..9022
                     /gene="IRES"
                     /label=IRES
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     CDS             9023..9616
                     /codon_start=1
                     /gene="Puro"
                     /label=Puro
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     CDS             complement(11272..12203)
                     /gene="Amp'"
                     /label=Amp'
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgacta aaatgccctg ccagtcatta
      601 caaccagaac caataaacac cccaacacac acaaaacaac agttgaaggc atccctgggg
      661 aaagtaggtg tgaaagaaga gctcctagca gtcggcaagt tcacacggac gtcaggggag
      721 accacgcaca cgcacagaga gccagcagga gatggcaaga gcatcagaac gtttaaggag
      781 tctccaaagc agatcctgga cccagcagcc cgtgtaactg gaatgaagaa gtggccaaga
      841 acgcctaagg aagaggccca gtcactagaa gacctggctg gcttcaaaga gctcttccag
      901 acaccaggtc cctctgagga atcaatgact gatgagaaaa ctaccaaaat agcctgcaaa
      961 tctccaccac cagaatcagt ggacactcca acaagcacaa agcaatggcc taagagaagt
     1021 ctcaggaaag cagatgtaga ggaagaattc ttagcactca ggaaactaac accatcagca
     1081 gggaaagcca tgcttacgcc caaaccagca ggaggtgatg agaaagacat taaagcattt
     1141 atgggaactc cagtgcagaa actggacctg gcaggaactt tacctggcag caaaagacag
     1201 ctacagactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt taaagagctc
     1261 ttccagactc ctggtcacac cgaggaatta gtggctgctg gtaaaaccac taaaataccc
     1321 tgcgactctc cacagtcaga cccagtggac accccaacaa gcacaaagca acgacccaag
     1381 agaagtatca ggaaagcaga tgtagaggga gaactcttag cgtgcaggaa tctaatgcca
     1441 tcagcaggca aagccatgca cacgcctaaa ccatcagtag gtgaagagaa agacatcatc
     1501 atatttgtgg gaactccagt gcagaaactg gacctgacag agaacttaac cggcagcaag
     1561 agacggccac aaactcctaa ggaagaggcc caggctctgg aagacctgac tggctttaaa
     1621 gagctcttcc agacccctgg tcatactgaa gaagcagtgg ctgctggcaa aactactaaa
     1681 atgccctgcg aatcttctcc accagaatca gcagacaccc caacaagcac aagaaggcag
     1741 cccaagacac ctttggagaa aagggacgta cagaaggagc tctcagccct gaagaagctc
     1801 acacagacat caggggaaac cacacacaca gataaagtac caggaggtga ggataaaagc
     1861 atcaacgcgt ttagggaaac tgcaaaacag aaactggacc cagcagcaag tgtaactggt
     1921 agcaagaggc acccaaaaac taaggaaaag gcccaacccc tagaagacct ggctggcttg
     1981 aaagagctct tccagacacc agtatgcact gacaagccca cgactcacga gaaaactacc
     2041 aaaatagcct gcagatcaca accagaccca gtggacacac caacaagctc caagccacag
     2101 tccaagagaa gtctcaggaa agtggacgta gaagaagaat tcttcgcact caggaaacga
     2161 acaccatcag caggcaaagc catgcacaca cccaaaccag cagtaagtgg tgagaaaaac
     2221 atctacgcat ttatgggaac tccagtgcag aaactggacc tgacagagaa cttaactggc
     2281 agcaagagac ggctacaaac tcctaaggaa aaggcccagg ctctagaaga cctggctggc
     2341 tttaaagagc tcttccagac acgaggtcac actgaggaat caatgactaa tgataaaact
     2401 gccaaagtag cctgcaaatc ttcacaacca gacccagaca aaaacccagc aagctccaag
     2461 cgacggctca agacatccct ggggaaagtg ggcgtgaaag aagagctcct agcagttggc
     2521 aagctcacac agacatcagg agagactaca cacacacaca cagagccaac aggagatggt
     2581 aagagcatga aagcatttat ggagtctcca aagcagatct tagactcagc agcaagtcta
     2641 actggcagca agaggcagct gagaactcct aagggaaagt ctgaagtccc tgaagacctg
     2701 gccggcttca tcgagctctt ccagacacca agtcacacta aggaatcaat gactaatgaa
     2761 aaaactacca aagtatccta cagagcttca cagccagacc tagtggacac cccaacaagc
     2821 tccaagccac agcccaagag aagtctcagg aaagcagaca ctgaagaaga atttttagca
     2881 tttaggaaac aaacgccatc agcaggcaaa gccatgcaca cacccaaacc agcagtaggt
     2941 gaagagaaag acatcaacac gtttttggga actccagtgc agaaactgga ccagccagga
     3001 aatttacctg gcagcaatag acggctacaa actcgtaagg aaaaggccca ggctctagaa
     3061 gaactgactg gcttcagaga gcttttccag acaccatgca ctgataaccc cacgactgat
     3121 gagaaaacta ccaaaaaaat actctgcaaa tctccgcaat cagacccagc ggacacccca
     3181 acaaacacaa agcaacggcc caagagaagc ctcaagaaag cagacgtaga ggaagaattt
     3241 ttagcattca ggaaactaac accatcagca ggcaaagcca tgcacacgcc taaagcagca
     3301 gtaggtgaag agaaagacat caacacattt gtggggactc cagtggagaa actggacctg
     3361 ctaggaaatt tacctggcag caagagacgg ccacaaactc ctaaagaaaa ggccaaggct
     3421 ctagaagatc tggctggctt caaagagctc ttccagacac caggtcacac tgaggaatca
     3481 atgaccgatg acaaaatcac agaagtatcc tgcaaatctc cacaaccaga cccagtcaaa
     3541 accccaacaa gctccaagca acgactcaag atatccttgg ggaaagtagg tgtgaaagaa
     3601 gaggtcctac cagtcggcaa gctcacacag acgtcaggga agaccacaca gacacacaga
     3661 gagacagcag gagatggaaa gagcatcaaa gcgtttaagg aatctgcaaa gcagatgctg
     3721 gacccagcaa actatggaac tgggatggag aggtggccaa gaacacctaa ggaagaggcc
     3781 caatcactag aagacctggc cggcttcaaa gagctcttcc agacaccaga ccacactgag
     3841 gaatcaacaa ctgatgacaa aactaccaaa atagcctgca aatctccacc accagaatca
     3901 atggacactc caacaagcac aaggaggcgg cccaaaacac ctttggggaa aagggatata
     3961 gtggaagagc tctcagccct gaagcagctc acacagacca cacacacaga caaagtacca
     4021 ggagatgagg ataaaggcat caacgtgttc agggaaactg caaaacagaa actggaccca
     4081 gcagcaagtg taactggtag caagaggcag ccaagaactc ctaagggaaa agcccaaccc
     4141 ctagaagact tggctggctt gaaagagctc ttccagacac caatatgcac tgacaagccc
     4201 acgactcatg agaaaactac caaaatagcc tgcagatctc cacaaccaga cccagtgggt
     4261 accccaacaa tcttcaagcc acagtccaag agaagtctca ggaaagcaga cgtagaggaa
     4321 gaatccttag cactcaggaa acgaacacca tcagtaggga aagctatgga cacacccaaa
     4381 ccagcaggag gtgatgagaa agacatgaaa gcatttatgg gaactccagt gcagaaattg
     4441 gacctgccag gaaatttacc tggcagcaaa agatggccac aaactcctaa ggaaaaggcc
     4501 caggctctag aagacctggc tggcttcaaa gagctcttcc agacaccagg cactgacaag
     4561 cccacgactg atgagaaaac taccaaaata gcctgcaaat ctccacaacc agacccagtg
     4621 gacaccccag caagcacaaa gcaacggccc aagagaaacc tcaggaaagc agacgtagag
     4681 gaagaatttt tagcactcag gaaacgaaca ccatcagcag gcaaagccat ggacacacca
     4741 aaaccagcag taagtgatga gaaaaatatc aacacatttg tggaaactcc agtgcagaaa
     4801 ctggacctgc taggaaattt acctggcagc aagagacagc cacagactcc taaggaaaag
     4861 gctgaggctc tagaggacct ggttggcttc aaagaactct tccagacacc aggtcacact
     4921 gaggaatcaa tgactgatga caaaatcaca gaagtatcct gtaaatctcc acagccagag
     4981 tcattcaaaa cctcaagaag ctccaagcaa aggctcaaga tacccctggt gaaagtggac
     5041 atgaaagaag agcccctagc agtcagcaag ctcacacgga catcagggga gactacgcaa
     5101 acacacacag agccaacagg agatagtaag agcatcaaag cgtttaagga gtctccaaag
     5161 cagatcctgg acccagcagc aagtgtaact ggtagcagga ggcagctgag aactcgtaag
     5221 gaaaaggccc gtgctctaga agacctggtt gacttcaaag agctcttctc agcaccaggt
     5281 cacactgaag agtcaatgac tattgacaaa aacacaaaaa ttccctgcaa atctccccca
     5341 ccagaactaa cagacactgc cacgagcaca aagagatgcc ccaagacacg tcccaggaaa
     5401 gaagtaaaag aggagctctc agcagttgag aggctcacgc aaacatcagg gcaaagcaca
     5461 cacacacaca aagaaccagc aagcggtgat gagggcatca aagtattgaa gcaacgtgca
     5521 aagaagaaac caaacccagt agaagaggaa cccagcagga gaaggccaag agcacctaag
     5581 gaaaaggccc aacccctgga agacctggcc ggcttcacag agctctctga aacatcaggt
     5641 cacactcagg aatcactgac tgctggcaaa gccactaaaa taccctgcga atctccccca
     5701 ctagaagtgg tagacaccac agcaagcaca aagaggcatc tcaggacacg tgtgcagaag
     5761 gtacaagtaa aagaagagcc ttcagcagtc aagttcacac aaacatcagg ggaaaccacg
     5821 gatgcagaca aagaaccagc aggtgaagat aaaggcatca aagcattgaa ggaatctgca
     5881 aaacagacac cggctccagc agcaagtgta actggcagca ggagacggcc aagagcaccc
     5941 agggaaagtg cccaagccat agaagaccta gctggcttca aagacccagc agcaggtcac
     6001 actgaagaat caatgactga tgacaaaacc actaaaatac cctgcaaatc atcaccagaa
     6061 ctagaagaca ccgcaacaag ctcaaagaga cggcccagga cacgtgccca gaaagtagaa
     6121 gtgaaggagg agctgttagc agttggcaag ctcacacaaa cctcagggga gaccacgcac
     6181 accgacaaag agccggtagg tgagggcaaa ggcacgaaag catttaagca acctgcaaag
     6241 cggaagctgg acgcagaaga tgtaattggc agcaggagac agccaagagc acctaaggaa
     6301 aaggcccaac ccctggaaga tctggccagc ttccaagagc tctctcaaac accaggccac
     6361 actgaggaac tggcaaatgg tgctgctgat agctttacaa gcgctccaaa gcaaacacct
     6421 gacagtggaa aacctctaaa aatatccaga agagttcttc gggcccctaa agtagaaccc
     6481 gtgggagacg tggtaagcac cagagaccct gtaaaatcac aaagcaaaag caacacttcc
     6541 ctgcccccac tgcccttcaa gaggggaggt ggcaaagatg gaagcgtcac gggaaccaag
     6601 aggttgcgct gcatgccagc accagaggaa attgtggagg agctgccagc cagcaagaag
     6661 cagagggttg ctcccagggc aagaggcaaa tcatccgaac ccgtggtcat catgaagaga
     6721 agtttgagga cttctgcaaa aagaattgaa cctgcggaag agctgaacag caacgacatg
     6781 aaaaccaaca aagaggaaca caaattacaa gactcggtcc ctgaaaataa gggaatatcc
     6841 ctgcgctcca gacgccaaaa taagactgag gcagaacagc aaataactga ggtctttgta
     6901 ttagcagaaa gaatagaaat aaacagaaat gaaaagaagc ccatgaagac ctccccagag
     6961 atggacattc agaatccaga tgatggagcc cggaaaccca tacctagaga caaagtcact
     7021 gagaacaaaa ggtgcttgag gtctgctaga cagaatgaga gctcccagcc taaggtggca
     7081 gaggagagcg gagggcagaa gagtgcgaag gttctcatgc agaatcagaa agggaaagga
     7141 gaagcaggaa attcagactc catgtgcctg agatcaagaa agacaaaaag ccagcctgca
     7201 gcaagcactt tggagagcaa atctgtgcag agagtaacgc ggagtgtcaa gaggtgtgca
     7261 gaaaatccaa agaaggctga ggacaatgtg tgtgtcaaga aaataagaac cagaagtcat
     7321 agggacagtg aagatatttg ggatcctatg gtgagcaagg gcgaggagga taacatggcc
     7381 tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac
     7441 atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc
     7501 accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc
     7561 catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc
     7621 tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac
     7681 taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt
     7741 ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg
     7801 aagaagactt accccaacga caaaaccatc atcagtacct ttaagtggag ttacaccact
     7861 ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg
     7921 gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga gctcaagcac
     7981 tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt gatgggcatg
     8041 gacgagctgt acaagtaagc ggccgcatag ataactgatc cagtgtgctg gaattaattc
     8101 gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc
     8161 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt
     8221 gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag
     8281 cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt
     8341 gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg
     8401 gcggccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     8461 aataaggccg gtgtgcgttt gtctatatgt gattttccac catattgccg tcttttggca
     8521 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8581 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8641 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8701 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8761 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8821 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8881 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8941 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     9001 cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9061 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9121 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9181 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     9241 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9301 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9361 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9421 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9481 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9541 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac
     9601 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     9661 acgaccccat ggctccgacc gaagccgacc cgggcggccc cgccgacccc gcacccgccc
     9721 ccgaggccca ccgactctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     9781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     9841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     9901 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     9961 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgagt
    10021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    10081 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10681 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
    10741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10921 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    11041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    11101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    11161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    12001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    12061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    12121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    12181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12241 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    12301 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    12361 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    12421 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
    12481 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
    12541 acggggtcat tagttcatag cccatatatg gagttc
//
