LOCUS       Exported                9669 bp ds-DNA     circular SYN 09-MAR-2022
DEFINITION  Ki67_Half rotated to position 5808.
ACCESSION   .
VERSION     .
KEYWORDS    r-Ki67_Half
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Vector molecule:  KI67_long_mNeonGreen_IRESpuro2*
               Fragment ends: BamHI and BstBI
               Fragment size: 5804
            Insert molecule:  Ki67_Half*
               Fragment ends: BstBI and BamHI
               Fragment size: 3865
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(573..9669,1..572)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..502
                     /label=CMV'
     primer_bind     538..557
                     /label=SC-226
                     /note="cloning_Ki-67_trunc_without_repeats_1_fw"
     primer_bind     566..606
                     /label=SC-220
                     /note="cloning_Ki-67_trunc_LR_half_repeats_fw"
     misc_feature    573..4431
                     /gene="MKI67"
                     /label='MKI67
                     /note="/translation=""MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDI
                      
                     RIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFR
                      
                     YENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNP
                      
                     QVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSR
                      
                     YGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATE
                      
                     KESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKA
                      
                     VGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLT
                      
                     PRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLT
                      
                     QVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFG
                      
                     GHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIH
                      
                     VEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSK
                      
                     RVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMN
                      
                     KRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQK
                      
                     MDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSES
                      
                     FGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTET
                      
                     EPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEM
                      
                     KEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTAR
                      
                     GQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLA
                      
                     VGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSL
                      
                     EDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEE
                      
                     EFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEK
                      
                     AQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKA
                      
                     DVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQT
                      
                     PKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTP
                      
                     LEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKR
                      
                     HPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSK
                      
                     RSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGS
                      
                     KRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSK
                      
                     RRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAAS
                      
                     LTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTP
                      
                     TSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLD
                      
                     QPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDP
                      
                     ADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPV
                      
                     EKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSP
                      
                     QPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFK
                      
                     ESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIA
                      
                     CKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFR
                      
                     ETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIA
                      
                     CRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDM
                      
                     KAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTT
                      
                     KIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDE
                      
                     KNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTD
                      
                     DKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEP
                      
                     TGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEE
                      
                     SMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTH
                      
                     KEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHT
                      
                     QESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTD
                      
                     ADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGH
                      
                     TEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETT
                      
                     HTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTP
                      
                     GHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKS
                      
                     NTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVV
                      
                     IMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQI
                      
                     TEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNES
                      
                     SQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVT
                      RSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI"""
     CDS             574..5151
                     /codon_start=1
                     /label=Ki67_Half
                     /translation="MTDDKITEVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPV
                     GKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLE
                     DLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVEE
                     LSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQPLE
                     DLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADVEEE
                     SLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTPKEKA
                     QALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADV
                     EEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPK
                     EKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLV
                     KVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQL
                     RTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPK
                     TRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRR
                     RPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRH
                     LRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTG
                     SRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRR
                     PRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIG
                     SRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKIS
                     RRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGGGKDGSVTGTKRLRCMPAP
                     EEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTSAKRIEPAEELNSNDMKTNKEEH
                     KLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAERIEINRNEKKPMKTSPEMDIQNP
                     DDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNS
                     DSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAENPKKAEDNVCVKKIRTRSHRDSE
                     DIWDPMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKG
                     DLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYR
                     YTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTG
                     NGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGM
                     DELYK"
     primer_bind     3450..3473
                     /label=SC-228
                     /note="cloning_Ki-67_trunc_without_repeats_2_fw"
     primer_bind     3454..3475
                     /label=SC-217
                     /note="cloning_Ki-67_trunc_LR_only_fw"
     misc_feature    3697
                     /label=silent mutation 2
                     /note="C -> T mutation"
     CDS             4435..5151
                     /codon_start=1
                     /gene="MKI67-mNeonGreen"
                     /label='MKI67-mNeonGreen
                     /translation="DPMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDG
                     YEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQF
                     EDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTI
                     ISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKE
                     WQKAFTDVMGMDELYK"
     primer_bind     complement(4439..4458)
                     /label=SC-218
                     /note="cloning_Ki-67_trunc_LR_only_rv"
     misc_feature    5184..5479
                     /label=IVS
     misc_feature    5505..6115
                     /label=IRES
     misc_feature    6116..6709
                     /label=Puro
     misc_feature    complement(8365..9296)
                     /label=Amp'
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgaccg atgacaaaat cacagaagta
      601 tcctgcaaat ctccacaacc agacccagtc aaaaccccaa caagctccaa gcaacgactc
      661 aagatatcct tggggaaagt aggtgtgaaa gaagaggtcc taccagtcgg caagctcaca
      721 cagacgtcag ggaagaccac acagacacac agagagacag caggagatgg aaagagcatc
      781 aaagcgttta aggaatctgc aaagcagatg ctggacccag caaactatgg aactgggatg
      841 gagaggtggc caagaacacc taaggaagag gcccaatcac tagaagacct ggccggcttc
      901 aaagagctct tccagacacc agaccacact gaggaatcaa caactgatga caaaactacc
      961 aaaatagcct gcaaatctcc accaccagaa tcaatggaca ctccaacaag cacaaggagg
     1021 cggcccaaaa cacctttggg gaaaagggat atagtggaag agctctcagc cctgaagcag
     1081 ctcacacaga ccacacacac agacaaagta ccaggagatg aggataaagg catcaacgtg
     1141 ttcagggaaa ctgcaaaaca gaaactggac ccagcagcaa gtgtaactgg tagcaagagg
     1201 cagccaagaa ctcctaaggg aaaagcccaa cccctagaag acttggctgg cttgaaagag
     1261 ctcttccaga caccaatatg cactgacaag cccacgactc atgagaaaac taccaaaata
     1321 gcctgcagat ctccacaacc agacccagtg ggtaccccaa caatcttcaa gccacagtcc
     1381 aagagaagtc tcaggaaagc agacgtagag gaagaatcct tagcactcag gaaacgaaca
     1441 ccatcagtag ggaaagctat ggacacaccc aaaccagcag gaggtgatga gaaagacatg
     1501 aaagcattta tgggaactcc agtgcagaaa ttggacctgc caggaaattt acctggcagc
     1561 aaaagatggc cacaaactcc taaggaaaag gcccaggctc tagaagacct ggctggcttc
     1621 aaagagctct tccagacacc aggcactgac aagcccacga ctgatgagaa aactaccaaa
     1681 atagcctgca aatctccaca accagaccca gtggacaccc cagcaagcac aaagcaacgg
     1741 cccaagagaa acctcaggaa agcagacgta gaggaagaat ttttagcact caggaaacga
     1801 acaccatcag caggcaaagc catggacaca ccaaaaccag cagtaagtga tgagaaaaat
     1861 atcaacacat ttgtggaaac tccagtgcag aaactggacc tgctaggaaa tttacctggc
     1921 agcaagagac agccacagac tcctaaggaa aaggctgagg ctctagagga cctggttggc
     1981 ttcaaagaac tcttccagac accaggtcac actgaggaat caatgactga tgacaaaatc
     2041 acagaagtat cctgtaaatc tccacagcca gagtcattca aaacctcaag aagctccaag
     2101 caaaggctca agatacccct ggtgaaagtg gacatgaaag aagagcccct agcagtcagc
     2161 aagctcacac ggacatcagg ggagactacg caaacacaca cagagccaac aggagatagt
     2221 aagagcatca aagcgtttaa ggagtctcca aagcagatcc tggacccagc agcaagtgta
     2281 actggtagca ggaggcagct gagaactcgt aaggaaaagg cccgtgctct agaagacctg
     2341 gttgacttca aagagctctt ctcagcacca ggtcacactg aagagtcaat gactattgac
     2401 aaaaacacaa aaattccctg caaatctccc ccaccagaac taacagacac tgccacgagc
     2461 acaaagagat gccccaagac acgtcccagg aaagaagtaa aagaggagct ctcagcagtt
     2521 gagaggctca cgcaaacatc agggcaaagc acacacacac acaaagaacc agcaagcggt
     2581 gatgagggca tcaaagtatt gaagcaacgt gcaaagaaga aaccaaaccc agtagaagag
     2641 gaacccagca ggagaaggcc aagagcacct aaggaaaagg cccaacccct ggaagacctg
     2701 gccggcttca cagagctctc tgaaacatca ggtcacactc aggaatcact gactgctggc
     2761 aaagccacta aaataccctg cgaatctccc ccactagaag tggtagacac cacagcaagc
     2821 acaaagaggc atctcaggac acgtgtgcag aaggtacaag taaaagaaga gccttcagca
     2881 gtcaagttca cacaaacatc aggggaaacc acggatgcag acaaagaacc agcaggtgaa
     2941 gataaaggca tcaaagcatt gaaggaatct gcaaaacaga caccggctcc agcagcaagt
     3001 gtaactggca gcaggagacg gccaagagca cccagggaaa gtgcccaagc catagaagac
     3061 ctagctggct tcaaagaccc agcagcaggt cacactgaag aatcaatgac tgatgacaaa
     3121 accactaaaa taccctgcaa atcatcacca gaactagaag acaccgcaac aagctcaaag
     3181 agacggccca ggacacgtgc ccagaaagta gaagtgaagg aggagctgtt agcagttggc
     3241 aagctcacac aaacctcagg ggagaccacg cacaccgaca aagagccggt aggtgagggc
     3301 aaaggcacga aagcatttaa gcaacctgca aagcggaagc tggacgcaga agatgtaatt
     3361 ggcagcagga gacagccaag agcacctaag gaaaaggccc aacccctgga agatctggcc
     3421 agcttccaag agctctctca aacaccaggc cacactgagg aactggcaaa tggtgctgct
     3481 gatagcttta caagcgctcc aaagcaaaca cctgacagtg gaaaacctct aaaaatatcc
     3541 agaagagttc ttcgggcccc taaagtagaa cccgtgggag acgtggtaag caccagagac
     3601 cctgtaaaat cacaaagcaa aagcaacact tccctgcccc cactgccctt caagagggga
     3661 ggtggcaaag atggaagcgt cacgggaacc aagaggttgc gctgcatgcc agcaccagag
     3721 gaaattgtgg aggagctgcc agccagcaag aagcagaggg ttgctcccag ggcaagaggc
     3781 aaatcatccg aacccgtggt catcatgaag agaagtttga ggacttctgc aaaaagaatt
     3841 gaacctgcgg aagagctgaa cagcaacgac atgaaaacca acaaagagga acacaaatta
     3901 caagactcgg tccctgaaaa taagggaata tccctgcgct ccagacgcca aaataagact
     3961 gaggcagaac agcaaataac tgaggtcttt gtattagcag aaagaataga aataaacaga
     4021 aatgaaaaga agcccatgaa gacctcccca gagatggaca ttcagaatcc agatgatgga
     4081 gcccggaaac ccatacctag agacaaagtc actgagaaca aaaggtgctt gaggtctgct
     4141 agacagaatg agagctccca gcctaaggtg gcagaggaga gcggagggca gaagagtgcg
     4201 aaggttctca tgcagaatca gaaagggaaa ggagaagcag gaaattcaga ctccatgtgc
     4261 ctgagatcaa gaaagacaaa aagccagcct gcagcaagca ctttggagag caaatctgtg
     4321 cagagagtaa cgcggagtgt caagaggtgt gcagaaaatc caaagaaggc tgaggacaat
     4381 gtgtgtgtca agaaaataag aaccagaagt catagggaca gtgaagatat ttgggatcct
     4441 atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac
     4501 atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca
     4561 aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc
     4621 tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg
     4681 atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg
     4741 cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac
     4801 atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc
     4861 aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa cgacaaaacc
     4921 atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta ccggagcact
     4981 gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa gaaccagccg
     5041 atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa cttcaaggag
     5101 tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaagta agcggccgca
     5161 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
     5221 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     5281 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     5341 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     5401 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     5461 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccctccc
     5521 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5581 tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5641 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5701 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5761 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5821 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5881 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5941 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6001 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6061 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     6121 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6181 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6241 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6301 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6361 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6421 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6481 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6541 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6601 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6661 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6721 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catggctccg accgaagccg
     6781 acccgggcgg ccccgccgac cccgcacccg cccccgaggc ccaccgactc tagagctcgc
     6841 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6901 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6961 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7021 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7081 tctgaggcgg aaagaaccag ctggggctcg agtgcattct agttgtggtt tgtccaaact
     7141 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7801 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8041 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9361 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
     9421 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     9481 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     9541 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     9601 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9661 atggagttc
//
