LOCUS       Exported               16330 bp ds-DNA     circular SYN 09-MAR-2022
DEFINITION  Ligation of PCR* into EGFP-Ki-67_IRESpuro2*.
ACCESSION   .
VERSION     .
KEYWORDS    EGFP-mCherry-Ki-67_IRESpuro2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Vector molecule:  EGFP-Ki-67_IRESpuro2*
               Fragment ends: BsrGI
               Fragment size: 14541
            Insert molecule:  PCR*
               Fragment ends: Pfl23II
               Fragment size: 1789
FEATURES             Location/Qualifiers
     source          1..16330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(4531..16330,1..4530)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..502
                     /label=CMV'
     primer_bind     3..25
                     /label=MWGS453
                     /note="mPBase_1.FWD"
     primer_bind     340..359
                     /label=SC-06
                     /note="pCMV promoter"
     primer_bind     504..523
                     /label=MWGS243
                     /note="IDT-BG1.FOR"
     primer_bind     538..557
                     /label=SC-226
                     /note="cloning_Ki-67_trunc_without_repeats_1_fw"
     primer_bind     545..604
                     /label=SC-470
                     /note="1f-pIRESneo3-o(Vec) for Gibson, PCR on 1034, 
                     inserted to keep BstBI restriction site"
     primer_bind     566..596
                     /label=SC-458
                     /note="Amplify mCherry from plasmid 1038 with BstBI site"
     misc_feature    573..578
                     /label=Kozak
                     /note="Kozak"
                     /note="Kozak"
     misc_feature    574..579
                     /gene="MCS"
                     /product="Multiple cloning sites"
                     /label=MCS
                     /note="Multiple cloning sites"
                     /note="/dnas_title=""Multiple cloning sites"""
     CDS             579..11828
                     /codon_start=1
                     /label=EGFP-mCherry-Ki-67
                     /translation="MASVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LKEFVTAAGITLGMDELYGKGSVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEG
                     EGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFK
                     WERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMY
                     PEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTI
                     VEQYERAEGRHSTGGMDELYKSSGPSGSSSWPTRRLVTIKRSGVDGPHFPLSLSTCLFG
                     RGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVIT
                     IIDRSFRYENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITE
                     GKVSGNPQVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEIS
                     SVKLVSRYGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGL
                     QTDYATEKESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVES
                     VQTPSKAVGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFK
                     AGDKTLTPRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEP
                     FLTLWLTQVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLK
                     RRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQ
                     ESGSEIHVEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRK
                     SGNLPSKRVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKH
                     GPQRSMNKRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLN
                     NFISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEP
                     LLPTSESFGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLET
                     KTSDTETEPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQ
                     QRREGEMKEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTE
                     LMKDTARGQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVG
                     VKEELLAVGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTP
                     KEEAQSLEDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSL
                     RKADVEEEFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQ
                     LQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRP
                     KRSIRKADVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTG
                     SKRRPQTPKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTST
                     RRQPKTPLEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAA
                     SVTGSKRHPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPT
                     SSKPQSKRSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDL
                     TENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPD
                     KNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQ
                     ILDSAASLTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQ
                     PDLVDTPTSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLG
                     TPVQKLDQPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILC
                     KSPQSDPADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDIN
                     TFVGTPVEKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKIT
                     EVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDG
                     KSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTD
                     DKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDED
                     KGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTH
                     EKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPA
                     GGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKP
                     TTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTP
                     KPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGH
                     TEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGET
                     TQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFS
                     APGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTS
                     GQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTEL
                     SETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQ
                     TSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGF
                     KDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLT
                     QTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASF
                     QELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDP
                     VKSQSKSNTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARG
                     KSSEPVVIMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNK
                     TEAEQQITEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLR
                     SARQNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLES
                     KSVQRVTRSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDIWDRPHR"
     primer_bind     588..608
                     /label=SC-249
                     /note="Forward Primer for EGFP"
     primer_bind     588..605
                     /label=SC-463
                     /note="Amplify mCherry with N-terminal linker from plasmid 
                     492, BamHI site, 1 bp for keeping frame in plasmid 798"
     primer_bind     588..605
                     /label=SC-59
                     /note="mNeon_Green_BamHI_fw"
     CDS             589..1289
                     /gene="mEGFP"
                     /product="monomeric EGFP"
                     /label=mEGFP'
                     /note="monomeric EGFP"
                     /note="/dnas_title=""monomeric EGFP"""
     primer_bind     593..615
                     /label=SC-310
                     /note="Gibson oligo to amplify EGFP + Linker from 494 
                     (2f-494_NFLAP_-o(pCR2_MKI67))"
     primer_bind     1271..1290
                     /label=SC-287
                     /note="Foward oligo to amplify Geneblock SC-04"
     primer_bind     1295..1334
                     /label=SC-523
                     /note="binds 1368 at pos 13130 with additional BsiWI 
                     restriction site and """"GKGS"""" (K is missing from EGFP 
                     and GS is short linker)"
     primer_bind     1308..1327
                     /label=SC-470
                     /note="1f-pIRESneo3-o(Vec) for Gibson, PCR on 1034, 
                     inserted to keep BstBI restriction site"
     primer_bind     1308..1319
                     /label=SC-458
                     /note="Amplify mCherry from plasmid 1038 with BstBI site"
     misc_feature    1311..2003
                     /label='mCherry'
     primer_bind     1311..1328
                     /label=SC-463
                     /note="Amplify mCherry with N-terminal linker from plasmid 
                     492, BamHI site, 1 bp for keeping frame in plasmid 798"
     primer_bind     1311..1328
                     /label=SC-59
                     /note="mNeon_Green_BamHI_fw"
     primer_bind     complement(1323..1343)
                     /label=MWGS326
                     /note="mCherry_Seq.REV"
     primer_bind     complement(1334..1354)
                     /label=MWGS267
                     /note="mCherry_REV-SEQ-primer"
     primer_bind     complement(1453..1471)
                     /label=MWGS340
                     /note="mCherry-R.REV"
     primer_bind     1665..1685
                     /label=MWGS268
                     /note="mCherry_FOR-SEQ-primer"
     primer_bind     1994..2004
                     /label=SC-287
                     /note="Foward oligo to amplify Geneblock SC-04"
     misc_feature    2043..3083
                     /gene="MKI67"
                     /label='MKI67''''
                     /note="/translation=""MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDI
                      
                     RIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFR
                      
                     YENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNP
                      
                     QVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSR
                      
                     YGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATE
                      
                     KESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKA
                      
                     VGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLT
                      
                     PRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLT
                      
                     QVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFG
                      
                     GHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIH
                      
                     VEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSK
                      
                     RVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMN
                      
                     KRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQK
                      
                     MDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSES
                      
                     FGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTET
                      
                     EPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEM
                      
                     KEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTAR
                      
                     GQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLA
                      
                     VGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSL
                      
                     EDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEE
                      
                     EFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEK
                      
                     AQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKA
                      
                     DVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQT
                      
                     PKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTP
                      
                     LEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKR
                      
                     HPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSK
                      
                     RSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGS
                      
                     KRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSK
                      
                     RRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAAS
                      
                     LTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTP
                      
                     TSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLD
                      
                     QPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDP
                      
                     ADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPV
                      
                     EKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSP
                      
                     QPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFK
                      
                     ESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIA
                      
                     CKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFR
                      
                     ETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIA
                      
                     CRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDM
                      
                     KAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTT
                      
                     KIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDE
                      
                     KNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTD
                      
                     DKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEP
                      
                     TGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEE
                      
                     SMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTH
                      
                     KEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHT
                      
                     QESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTD
                      
                     ADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGH
                      
                     TEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETT
                      
                     HTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTP
                      
                     GHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKS
                      
                     NTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVV
                      
                     IMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQI
                      
                     TEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNES
                      
                     SQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVT
                      RSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI"""
     misc_feature    3084..4530
                     /gene="MKI67"
                     /label=''MKI67''
                     /note="/translation=""MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDI
                      
                     RIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFR
                      
                     YENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNP
                      
                     QVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSR
                      
                     YGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATE
                      
                     KESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKA
                      
                     VGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLT
                      
                     PRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLT
                      
                     QVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFG
                      
                     GHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIH
                      
                     VEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSK
                      
                     RVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMN
                      
                     KRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQK
                      
                     MDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSES
                      
                     FGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTET
                      
                     EPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEM
                      
                     KEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTAR
                      
                     GQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLA
                      
                     VGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSL
                      
                     EDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEE
                      
                     EFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEK
                      
                     AQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKA
                      
                     DVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQT
                      
                     PKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTP
                      
                     LEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKR
                      
                     HPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSK
                      
                     RSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGS
                      
                     KRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSK
                      
                     RRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAAS
                      
                     LTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTP
                      
                     TSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLD
                      
                     QPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDP
                      
                     ADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPV
                      
                     EKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSP
                      
                     QPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFK
                      
                     ESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIA
                      
                     CKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFR
                      
                     ETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIA
                      
                     CRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDM
                      
                     KAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTT
                      
                     KIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDE
                      
                     KNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTD
                      
                     DKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEP
                      
                     TGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEE
                      
                     SMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTH
                      
                     KEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHT
                      
                     QESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTD
                      
                     ADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGH
                      
                     TEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETT
                      
                     HTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTP
                      
                     GHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKS
                      
                     NTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVV
                      
                     IMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQI
                      
                     TEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNES
                      
                     SQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVT
                      RSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI"""
     primer_bind     3332..3351
                     /label=SC-82
                     /note="MKI67_seq3"
     primer_bind     3462..3481
                     /label=SC-272
                     /note="amplify Ki-67 genomic RASA region fw_PCR2"
     primer_bind     complement(3537..3581)
                     /label=SC-215
                     /note="Intoduce RASA mutation in Ki-67 by Quick change"
     primer_bind     3537..3581
                     /label=SC-216
                     /note="Intoduce RASA mutation in Ki-67 by Quick change"
     primer_bind     3542..3562
                     /label=SC-256
                     /note="CRISPR_Ki-67_RASA_guide3_a"
     primer_bind     complement(3543..3562)
                     /label=SC-257
                     /note="CRISPR_Ki-67_RASA_guide3_b"
     primer_bind     complement(3547..3567)
                     /label=SC-255
                     /note="CRISPR_Ki-67_RASA_guide2_b"
     primer_bind     3547..3566
                     /label=SC-254
                     /note="CRISPR_Ki-67_RASA_guide2_a"
     primer_bind     complement(3586..3627)
                     /label=SC-129
                     /note="MKI67_quick_T494C_antisense"
     primer_bind     3586..3627
                     /label=SC-128
                     /note="MKI67_quick_T494C_sense"
     primer_bind     complement(3657..3676)
                     /label=SC-280
                     /note="Sequencing RASA mutanat (binds pos12759 rv on Ki-67 
                     genomic)"
     primer_bind     3850..3869
                     /label=SC-83
                     /note="MKI67_seq4"
     primer_bind     3909..3922
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     3921..3945
                     /label=SC-233
                     /note="cloning_Ki-67_trunc_no_CD_CT_fw"
     primer_bind     4332..4351
                     /label=SC-84
                     /note="MKI67_seq5"
     primer_bind     complement(4524..4543)
                     /label=SC-265
                     /note="KI-67 reverse oligo (binds downstream of AflII 
                     site)"
     primer_bind     complement(4525..4555)
                     /label=SC-469
                     /note="2r-KI67_long_-o(Vec) for Gibson, PCR on 798"
     misc_feature    4531..11807
                     /gene="MKI67"
                     /label='MKI67
                     /note="/codon_start=3"
                     /note="/translation=""RQCIRENGNVAKTPRNTYKMTSLETKTSDTETEPSKT
                      
                     VSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEMKEIER
                      
                     PFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTARGQNLL
                      
                     QTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFT
                      
                     RTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAG
                      
                     FKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLAL
                      
                     RKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALE
                      
                     DLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGE
                      
                     LLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEA
                      
                     QALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRD
                      
                     VQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTK
                      
                     EKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRK
                      
                     VDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQ
                      
                     TPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKT
                      
                     SLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSK
                      
                     RQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKP
                      
                     QPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNL
                      
                     PGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPT
                      
                     NTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDL
                      
                     LGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPV
                      
                     KTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQ
                      
                     MLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPP
                      
                     PESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQ
                      
                     KLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQ
                      
                     PDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMG
                      
                     TPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACK
                      
                     SPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINT
                      
                     FVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITE
                      
                     VSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSK
                      
                     SIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTID
                      
                     KNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPAS
                      
                     GDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLT
                      
                     AGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEP
                      
                     AGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESM
                      
                     TDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKE
                      
                     PVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEE
                      
                     LANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLP
                      
                     PLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRS
                      
                     LRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFV
                      
                     LAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKV
                      
                     AEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKR
                      CAENPKKAEDNVCVKKIRTRSHRDSEDI"""
     primer_bind     4903..4922
                     /label=SC-85
                     /note="MKI67_seq6"
     primer_bind     complement(5004..5022)
                     /label=SC-236
                     /note="cloning_Ki-67_trunc_no_rep_NT_rv"
     primer_bind     5041..5065
                     /label=SC-219
                     /note="cloning_Ki-67_trunc_LR_all_repeats_fw"
     primer_bind     complement(5160..5179)
                     /label=SC-148
                     /note="MKI67_seq61"
     primer_bind     5212..5228
                     /label=SC-107
                     /note="MKI67_seq22"
     primer_bind     5293..5312
                     /label=SC-117
                     /note="MKI67_seq32"
     primer_bind     5341..5360
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     5413..5433
                     /label=SC-127
                     /note="MKI67_seq42"
     primer_bind     5464..5483
                     /label=SC-118
                     /note="MKI67_seq33"
     primer_bind     complement(5518..5535)
                     /label=SC-131
                     /note="MKI67_seq44"
     primer_bind     complement(5627..5646)
                     /label=SC-139
                     /note="MKI67_seq52"
     primer_bind     5707..5726
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     5841..5860
                     /label=SC-87
                     /note="MKI67_seq8"
     primer_bind     complement(5976..5995)
                     /label=SC-145
                     /note="MKI67_seq58"
     primer_bind     6073..6092
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     6204..6227
                     /label=SC-69
                     /note="MKI67 split-F3"
     primer_bind     6233..6255
                     /label=SC-70
                     /note="MKI67 split-F4"
     primer_bind     complement(6240..6259)
                     /label=SC-134
                     /note="MKI67_seq47"
     primer_bind     6269..6310
                     /label=SC-63
                     /note="MKI67 split-F"
     primer_bind     6282..6310
                     /label=SC-68
                     /note="MKI67-split-F2"
     primer_bind     6283..6310
                     /label=SC-65
                     /note="MKI67 split-F1"
     primer_bind     6422..6442
                     /label=SC-124
                     /note="MKI67_seq39"
     primer_bind     6436..6455
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     6456..6476
                     /label=SC-125
                     /note="MKI67_seq40"
     primer_bind     6456..6475
                     /label=SC-88
                     /note="MKI67_seq9"
     primer_bind     6460..6482
                     /label=SC-110
                     /note="MKI67_seq25"
     primer_bind     complement(6464..6486)
                     /label=SC-140
                     /note="MKI67_seq53"
     primer_bind     complement(6468..6511)
                     /label=SC-64
                     /note="MKI67 split-R"
     primer_bind     complement(6468..6490)
                     /label=SC-66
                     /note="MKI67 split-R1"
     primer_bind     6478..6497
                     /label=SC-119
                     /note="MKI67_seq34"
     primer_bind     6510..6531
                     /label=SC-121
                     /note="MKI67_seq36"
     primer_bind     complement(6521..6540)
                     /label=SC-130
                     /note="MKI67_seq43"
     primer_bind     6571..6586
                     /label=SC-87
                     /note="MKI67_seq8"
     primer_bind     6584..6603
                     /label=SC-120
                     /note="MKI67_seq35"
     primer_bind     complement(6593..6612)
                     /label=SC-135
                     /note="MKI67_seq48"
     primer_bind     complement(6610..6629)
                     /label=SC-131
                     /note="MKI67_seq44"
     primer_bind     complement(6614..6633)
                     /label=SC-136
                     /note="MKI67_seq49"
     primer_bind     complement(6658..6677)
                     /label=SC-144
                     /note="MKI67_seq57"
     primer_bind     complement(6676..6695)
                     /label=SC-137
                     /note="MKI67_seq50"
     primer_bind     complement(6690..6709)
                     /label=SC-132
                     /note="MKI67_seq45"
     primer_bind     complement(6695..6714)
                     /label=SC-133
                     /note="MKI67_seq46"
     primer_bind     6799..6818
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     6924..6943
                     /label=SC-89
                     /note="MKI67_seq10"
     primer_bind     complement(7105..7124)
                     /label=SC-152
                     /note="MKI67_seq65"
     primer_bind     7123..7142
                     /label=SC-114
                     /note="MKI67_seq29"
     primer_bind     complement(7150..7169)
                     /label=SC-151
                     /note="MKI67_seq64"
     primer_bind     complement(7175..7194)
                     /label=SC-150
                     /note="MKI67_seq63"
     primer_bind     7193..7212
                     /label=SC-90
                     /note="MKI67_seq11"
     misc_feature    7226
                     /label=silent mutation 1
                     /note="C -> T mutation"
     primer_bind     complement(7250..7269)
                     /label=SC-149
                     /note="MKI67_seq62"
     primer_bind     7302..7318
                     /label=SC-87
                     /note="MKI67_seq8"
     primer_bind     complement(7390..7409)
                     /label=SC-144
                     /note="MKI67_seq57"
     primer_bind     complement(7451..7470)
                     /label=SC-139
                     /note="MKI67_seq52"
     primer_bind     7655..7673
                     /label=SC-118
                     /note="MKI67_seq33"
     primer_bind     7669..7689
                     /label=SC-122
                     /note="MKI67_seq37"
     primer_bind     complement(7675..7694)
                     /label=SC-141
                     /note="MKI67_seq54"
     primer_bind     complement(7679..7698)
                     /label=SC-142
                     /note="MKI67_seq55"
     primer_bind     7745..7764
                     /label=SC-91
                     /note="MKI67_seq12"
     primer_bind     complement(7817..7836)
                     /label=SC-139
                     /note="MKI67_seq52"
     primer_bind     7899..7916
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     7949..7982
                     /label=SC-220
                     /note="cloning_Ki-67_trunc_LR_half_repeats_fw"
     primer_bind     7949..7967
                     /label=SC-221
                     /note="cloning_Ki-67_trunc_LR_half_repeats_fw_2"
     primer_bind     8074..8093
                     /label=SC-106
                     /note="MKI67_seq21"
     primer_bind     complement(8082..8097)
                     /label=SC-148
                     /note="MKI67_seq61"
     primer_bind     8131..8150
                     /label=SC-107
                     /note="MKI67_seq22"
     primer_bind     8175..8196
                     /label=SC-100
                     /note="MKI67_quick_siRNA131_sense"
     primer_bind     8190..8209
                     /label=SC-108
                     /note="MKI67_seq23"
     primer_bind     8235..8254
                     /label=SC-92
                     /note="MKI67_seq13"
     primer_bind     8263..8282
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     8335..8355
                     /label=SC-127
                     /note="MKI67_seq42"
     primer_bind     8466..8488
                     /label=SC-65
                     /note="MKI67 split-F1"
     primer_bind     8466..8488
                     /label=SC-68
                     /note="MKI67-split-F2"
     primer_bind     8466..8488
                     /label=SC-63
                     /note="MKI67 split-F"
     primer_bind     8565..8584
                     /label=SC-109
                     /note="MKI67_seq24"
     primer_bind     8581..8601
                     /label=SC-123
                     /note="MKI67_seq38"
     primer_bind     8603..8623
                     /label=SC-124
                     /note="MKI67_seq39"
     primer_bind     8620..8636
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     8637..8657
                     /label=SC-125
                     /note="MKI67_seq40"
     primer_bind     8637..8656
                     /label=SC-88
                     /note="MKI67_seq9"
     primer_bind     8641..8663
                     /label=SC-110
                     /note="MKI67_seq25"
     primer_bind     complement(8649..8692)
                     /label=SC-64
                     /note="MKI67 split-R"
     primer_bind     complement(8649..8671)
                     /label=SC-66
                     /note="MKI67 split-R1"
     primer_bind     8659..8679
                     /label=SC-126
                     /note="MKI67_seq41"
     primer_bind     8689..8709
                     /label=SC-127
                     /note="MKI67_seq42"
     primer_bind     8755..8770
                     /label=SC-87
                     /note="MKI67_seq8"
     primer_bind     8775..8794
                     /label=SC-93
                     /note="MKI67_seq14"
     primer_bind     complement(8821..8840)
                     /label=SC-138
                     /note="MKI67_seq51"
     primer_bind     complement(8842..8856)
                     /label=SC-144
                     /note="MKI67_seq57"
     primer_bind     complement(8903..8922)
                     /label=SC-139
                     /note="MKI67_seq52"
     primer_bind     8983..9002
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     9007..9026
                     /label=SC-110
                     /note="MKI67_seq25"
     primer_bind     complement(9011..9030)
                     /label=SC-140
                     /note="MKI67_seq53"
     primer_bind     9022..9042
                     /label=SC-126
                     /note="MKI67_seq41"
     primer_bind     9052..9072
                     /label=SC-127
                     /note="MKI67_seq42"
     primer_bind     9103..9122
                     /label=SC-118
                     /note="MKI67_seq33"
     primer_bind     9110..9129
                     /label=SC-94
                     /note="MKI67_seq15"
     primer_bind     9114..9133
                     /label=SC-111
                     /note="MKI67_seq26"
     primer_bind     9118..9138
                     /label=SC-122
                     /note="MKI67_seq37"
     primer_bind     complement(9124..9143)
                     /label=SC-141
                     /note="MKI67_seq54"
     primer_bind     complement(9128..9147)
                     /label=SC-142
                     /note="MKI67_seq55"
     primer_bind     complement(9197..9216)
                     /label=SC-143
                     /note="MKI67_seq56"
     primer_bind     complement(9205..9224)
                     /label=SC-144
                     /note="MKI67_seq57"
     primer_bind     complement(9249..9268)
                     /label=SC-145
                     /note="MKI67_seq58"
     primer_bind     complement(9266..9285)
                     /label=SC-139
                     /note="MKI67_seq52"
     primer_bind     9398..9416
                     /label=SC-221
                     /note="cloning_Ki-67_trunc_LR_half_repeats_fw_2"
     primer_bind     complement(9430..9449)
                     /label=SC-146
                     /note="MKI67_seq59"
     primer_bind     9436..9455
                     /label=SC-112
                     /note="MKI67_seq27"
     primer_bind     complement(9463..9482)
                     /label=SC-147
                     /note="MKI67_seq60"
     primer_bind     9463..9482
                     /label=SC-113
                     /note="MKI67_seq28"
     primer_bind     9524..9542
                     /label=SC-106
                     /note="MKI67_seq21"
     primer_bind     complement(9531..9550)
                     /label=SC-148
                     /note="MKI67_seq61"
     primer_bind     9576..9595
                     /label=SC-95
                     /note="MKI67_seq16"
     primer_bind     9670..9689
                     /label=SC-114
                     /note="MKI67_seq29"
     primer_bind     9703..9722
                     /label=SC-115
                     /note="MKI67_seq30"
     primer_bind     9712..9731
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     9880..9891
                     /label=SC-70
                     /note="MKI67 split-F4"
     primer_bind     9912..9946
                     /label=SC-63
                     /note="MKI67 split-F"
     primer_bind     9918..9946
                     /label=SC-68
                     /note="MKI67-split-F2"
     primer_bind     complement(10039..10060)
                     /label=SC-238
                     /note="cloning_Ki-67_trunc_without_LR_1_rv and BamHI site"
     primer_bind     10058..10078
                     /label=SC-124
                     /note="MKI67_seq39"
     primer_bind     10072..10091
                     /label=SC-86
                     /note="MKI67_seq7"
     primer_bind     10194..10213
                     /label=SC-96
                     /note="MKI67_seq17"
     primer_bind     10265..10306
                     /label=SC-63
                     /note="MKI67 split-F"
     primer_bind     complement(10672..10725)
                     /label=SC-101
                     /note="MKI67_quick_siRNA131_antisense"
     primer_bind     10672..10725
                     /label=SC-100
                     /note="MKI67_quick_siRNA131_sense"
     primer_bind     complement(10674..10724)
                     /label=SC-105
                     /note="MKI67_quick_siRNA131b_antisense"
     primer_bind     10674..10724
                     /label=SC-104
                     /note="MKI67_quick_siRNA131b_sense"
     primer_bind     10696..10715
                     /label=SC-97
                     /note="MKI67_seq18"
     primer_bind     10737..10756
                     /label=SC-109
                     /note="MKI67_seq24"
     primer_bind     complement(10756..10777)
                     /label=SC-238
                     /note="cloning_Ki-67_trunc_without_LR_1_rv and BamHI site"
     primer_bind     complement(10803..10825)
                     /label=SC-352
                     /note="Amplification of Ki-67 with SC-226 to generate no LR
                     fragment with BamHI site (8831 bp)"
     primer_bind     10829..10849
                     /label=SC-228
                     /note="cloning_Ki-67_trunc_without_repeats_2_fw"
     primer_bind     10830..10851
                     /label=SC-217
                     /note="cloning_Ki-67_trunc_LR_only_fw"
     primer_bind     10870..10890
                     /label=SC-237
                     /note="cloning_Ki-67_trunc_no_rep_CT_fw"
     primer_bind     complement(11050..11069)
                     /label=SC-355
                     /note="Sequencing LR domain reverse"
     primer_bind     complement(11065..11084)
                     /label=SC-356
                     /note="Sequencing LR domain reverse2"
     misc_feature    11073
                     /label=silent mutation 2
                     /note="C -> T mutation"
     primer_bind     11218..11237
                     /label=SC-98
                     /note="MKI67_seq19"
     primer_bind     11403..11422
                     /label=SC-361
                     /note="CD random 1 (pos 9329)"
     primer_bind     11570..11589
                     /label=SC-99
                     /note="MKI67_seq20"
     primer_bind     11771..11790
                     /label=SC-173
                     /note="CRISPR_Ki-67_C_term_guide8_a"
     primer_bind     complement(11774..11807)
                     /label=SC-191
                     /note="Vr-pCR4-TOPO-CRKi67Cterm_2PAMmut"
     primer_bind     complement(11774..11807)
                     /label=SC-184
                     /note="Vr-pCR4-TOPO-CRKi67Cterm"
     primer_bind     complement(11780..11807)
                     /label=SC-202
                     /note="Quick_change_C-MKI67_rv"
     primer_bind     complement(11780..11807)
                     /label=SC-72
                     /note="alternative to SC-67, 3 nts left out"
     primer_bind     complement(11782..11809)
                     /label=SC-71
                     /note="alternative to SC-67, more stable"
     primer_bind     complement(11782..11807)
                     /label=SC-74
                     /note="MKI67-full-R2"
     primer_bind     complement(11782..11801)
                     /label=SC-167
                     /note="CRISPR_Ki-67_C_term_guide1_a"
     primer_bind     complement(11785..11809)
                     /label=SC-62
                     /note="MKI67-pDONR223-R"
     primer_bind     complement(11787..11807)
                     /label=SC-73
                     /note="MKI67-full-R1"
     primer_bind     complement(11790..11809)
                     /label=SC-77
                     /note="MKI67-cDNA"
     primer_bind     complement(11830..11848)
                     /label=MWGS246
                     /note="IDT-GB2.REV"
     misc_feature    11845..12140
                     /label=IVS
     primer_bind     complement(11904..11923)
                     /label=SC-464
                     /note="Amplify mCherry with N-terminal linker from plasmid 
                     492, NotI site"
     primer_bind     complement(12054..12073)
                     /label=SC-05
                     /note="IRESvector_rev"
     primer_bind     12093..12112
                     /label=MWGS338
                     /note="pMT2-F.FOR"
     primer_bind     complement(12101..12115)
                     /label=SC-351
                     /note="Amplification of shRNA FKBP with HindlII sites 
                     shorter (reverse)"
     misc_feature    12166..12776
                     /label=IRES
     primer_bind     complement(12335..12352)
                     /label=MWGS339
                     /note="IRES_reverse.REV"
     primer_bind     12339..12358
                     /label=SC-08
                     /note="IRES_fw"
     primer_bind     12556..12575
                     /label=SC-09
                     /note="IRES_2_w"
     primer_bind     complement(12774..12793)
                     /label=MFDS_Puro_Rev
                     /note="Puro_Rev"
     misc_feature    12777..13370
                     /label=Puro
     primer_bind     13056..13075
                     /label=SC-10
                     /note="Puro_fw"
     primer_bind     complement(13259..13317)
                     /label=SC-463
                     /note="Amplify mCherry with N-terminal linker from plasmid 
                     492, BamHI site, 1 bp for keeping frame in plasmid 798"
     primer_bind     13578..13597
                     /label=SC-11
                     /note="vector backbone"
     primer_bind     complement(13916..13935)
                     /label=SC-289
                     /note="sequencig pCR2"
     primer_bind     complement(14096..14113)
                     /label=MWGS311
                     /note="L4440.SEQ"
     misc_feature    complement(15026..15957)
                     /label=Amp'
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaccgccat ggctagcgtg agcaagggcg
      601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      841 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      901 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      961 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1021 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1081 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1141 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1201 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtccttaag gagttcgtga
     1261 ccgccgccgg gatcactctc ggcatggacg agctgtacgg caagggaagc gtgagcaagg
     1321 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1381 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1441 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1501 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1561 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1621 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     1681 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     1741 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1801 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     1861 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     1921 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1981 gccactccac cggcggcatg gacgagctgt acaagagcag cggccccagc ggcagcagca
     2041 gctggcccac gagacgcctg gttactatca aaaggagcgg ggtcgacggt ccccactttc
     2101 ccctgagcct cagcacctgc ttgtttggaa ggggtattga atgtgacatc cgtatccagc
     2161 ttcctgttgt gtcaaaacaa cattgcaaaa ttgaaatcca tgagcaggag gcaatattac
     2221 ataatttcag ttccacaaat ccaacacaag taaatgggtc tgttattgat gagcctgtac
     2281 ggctaaaaca tggagatgta ataactatta ttgatcgttc cttcaggtat gaaaatgaaa
     2341 gtcttcagaa tggaaggaag tcaactgaat ttccaagaaa aatacgtgaa caggagccag
     2401 cacgtcgtgt ctcaagatct agcttctctt ctgaccctga tgagaaagct caagattcca
     2461 aggcctattc aaaaatcact gaaggaaaag tttcaggaaa tcctcaggta catatcaaga
     2521 atgtcaaaga agacagtacc gcagatgact caaaagacag tgttgctcag ggaacaacta
     2581 atgttcattc ctcagaacat gctggacgta atggcagaaa tgcagctgat cccatttctg
     2641 gggattttaa agaaatttcc agcgttaaat tagtgagccg ttatggagaa ttgaagtctg
     2701 ttcccactac acaatgtctt gacaatagca aaaaaaatga atctcccttt tggaagcttt
     2761 atgagtcagt gaagaaagag ttggatgtaa aatcacaaaa agaaaatgtc ctacagtatt
     2821 gtagaaaatc tggattacaa actgattacg caacagagaa agaaagtgct gatggtttac
     2881 agggggagac ccaactgttg gtctcgcgta agtcaagacc aaaatctggt gggagcggcc
     2941 acgctgtggc agagcctgct tcacctgaac aagagcttga ccagaacaag gggaagggaa
     3001 gagacgtgga gtctgttcag actcccagca aggctgtggg cgccagcttt cctctctatg
     3061 agccggctaa aatgaagacc cccgtacaat attcacagca acaaaattct ccacaaaaac
     3121 ataagaacaa agacctgtat actactggta gaagagaatc tgtgaatctg ggtaaaagtg
     3181 aaggcttcaa ggctggtgat aaaactctta ctcccaggaa gctttcaact agaaatcgaa
     3241 caccagctaa agttgaagat gcagctgact ctgccactaa gccagaaaat ctctcttcca
     3301 aaaccagagg aagtattcct acagatgtgg aagttctgcc tacggaaact gaaattcaca
     3361 atgagccatt tttaactctg tggctcactc aagttgagag gaagatccaa aaggattccc
     3421 tcagcaagcc tgagaaattg ggcactacag ctggacagat gtgctctggg ttacctggtc
     3481 ttagttcagt tgatatcaac aactttggtg attccattaa tgagagtgag ggaatacctt
     3541 tgaaaagaag gcgtgtgtcc tttggtgggc acctaagacc tgaactattt gatgaaaact
     3601 tgcctcctaa tacgcctctc aaaaggggag aagccccaac caaaagaaag tctctggtaa
     3661 tgcacactcc acctgtcctg aagaaaatca tcaaggaaca gcctcaacca tcaggaaaac
     3721 aagagtcagg ttcagaaatc catgtggaag tgaaggcaca aagcttggtt ataagccctc
     3781 cagctcctag tcctaggaaa actccagttg ccagtgatca acgccgtagg tcctgcaaaa
     3841 cagcccctgc ttccagcagc aaatctcaga cagaggttcc taagagagga gggagaaaga
     3901 gtggcaacct gccttcaaag agagtgtcta tcagccgaag tcaacatgat attttacaga
     3961 tgatatgttc caaaagaaga agtggtgctt cggaagcaaa tctgattgtt gcaaaatcat
     4021 gggcagatgt agtaaaactt ggtgcaaaac aaacacaaac taaagtcata aaacatggtc
     4081 ctcaaaggtc aatgaacaaa aggcaaagaa gacctgctac tccaaagaag cctgtgggcg
     4141 aagttcacag tcaatttagt acaggccacg caaactctcc ttgtaccata ataataggga
     4201 aagctcatac tgaaaaagta catgtgcctg ctcgacccta cagagtgctc aacaacttca
     4261 tttccaacca aaaaatggac tttaaggaag atctttcagg aatagctgaa atgttcaaga
     4321 ccccagtgaa ggagcaaccg cagttgacaa gcacatgtca catcgctatt tcaaattcag
     4381 agaatttgct tggaaaacag tttcaaggaa ctgattcagg agaagaacct ctgctcccca
     4441 cctcagagag ttttggagga aatgtgttct tcagtgcaca gaatgcagca aaacagccat
     4501 ctgataaatg ctctgcaagc cctcccttaa gacggcagtg tattagagaa aatggaaacg
     4561 tagcaaaaac gcccaggaac acctacaaaa tgacttctct ggagacaaaa acttcagata
     4621 ctgagacaga gccttcaaaa acagtatcca ctgcaaacag gtcaggaagg tctacagagt
     4681 tcaggaatat acagaagcta cctgtggaaa gtaagagtga agaaacaaat acagaaattg
     4741 ttgagtgcat cctaaaaaga ggtcagaagg caacactact acaacaaagg agagaaggag
     4801 agatgaagga aatagaaaga ccttttgaga catataagga aaatattgaa ttaaaagaaa
     4861 acgatgaaaa gatgaaagca atgaagagat caagaacttg ggggcagaaa tgtgcaccaa
     4921 tgtctgacct gacagacctc aagagcttgc ctgatacaga actcatgaaa gacacggcac
     4981 gtggccagaa tctcctccaa acccaagatc atgccaaggc accaaagagt gagaaaggca
     5041 aaatcactaa aatgccctgc cagtcattac aaccagaacc aataaacacc ccaacacaca
     5101 caaaacaaca gttgaaggca tccctgggga aagtaggtgt gaaagaagag ctcctagcag
     5161 tcggcaagtt cacacggacg tcaggggaga ccacgcacac gcacagagag ccagcaggag
     5221 atggcaagag catcagaacg tttaaggagt ctccaaagca gatcctggac ccagcagccc
     5281 gtgtaactgg aatgaagaag tggccaagaa cgcctaagga agaggcccag tcactagaag
     5341 acctggctgg cttcaaagag ctcttccaga caccaggtcc ctctgaggaa tcaatgactg
     5401 atgagaaaac taccaaaata gcctgcaaat ctccaccacc agaatcagtg gacactccaa
     5461 caagcacaaa gcaatggcct aagagaagtc tcaggaaagc agatgtagag gaagaattct
     5521 tagcactcag gaaactaaca ccatcagcag ggaaagccat gcttacgccc aaaccagcag
     5581 gaggtgatga gaaagacatt aaagcattta tgggaactcc agtgcagaaa ctggacctgg
     5641 caggaacttt acctggcagc aaaagacagc tacagactcc taaggaaaag gcccaggctc
     5701 tagaagacct ggctggcttt aaagagctct tccagactcc tggtcacacc gaggaattag
     5761 tggctgctgg taaaaccact aaaataccct gcgactctcc acagtcagac ccagtggaca
     5821 ccccaacaag cacaaagcaa cgacccaaga gaagtatcag gaaagcagat gtagagggag
     5881 aactcttagc gtgcaggaat ctaatgccat cagcaggcaa agccatgcac acgcctaaac
     5941 catcagtagg tgaagagaaa gacatcatca tatttgtggg aactccagtg cagaaactgg
     6001 acctgacaga gaacttaacc ggcagcaaga gacggccaca aactcctaag gaagaggccc
     6061 aggctctgga agacctgact ggctttaaag agctcttcca gacccctggt catactgaag
     6121 aagcagtggc tgctggcaaa actactaaaa tgccctgcga atcttctcca ccagaatcag
     6181 cagacacccc aacaagcaca agaaggcagc ccaagacacc tttggagaaa agggacgtac
     6241 agaaggagct ctcagccctg aagaagctca cacagacatc aggggaaacc acacacacag
     6301 ataaagtacc aggaggtgag gataaaagca tcaacgcgtt tagggaaact gcaaaacaga
     6361 aactggaccc agcagcaagt gtaactggta gcaagaggca cccaaaaact aaggaaaagg
     6421 cccaacccct agaagacctg gctggcttga aagagctctt ccagacacca gtatgcactg
     6481 acaagcccac gactcacgag aaaactacca aaatagcctg cagatcacaa ccagacccag
     6541 tggacacacc aacaagctcc aagccacagt ccaagagaag tctcaggaaa gtggacgtag
     6601 aagaagaatt cttcgcactc aggaaacgaa caccatcagc aggcaaagcc atgcacacac
     6661 ccaaaccagc agtaagtggt gagaaaaaca tctacgcatt tatgggaact ccagtgcaga
     6721 aactggacct gacagagaac ttaactggca gcaagagacg gctacaaact cctaaggaaa
     6781 aggcccaggc tctagaagac ctggctggct ttaaagagct cttccagaca cgaggtcaca
     6841 ctgaggaatc aatgactaat gataaaactg ccaaagtagc ctgcaaatct tcacaaccag
     6901 acccagacaa aaacccagca agctccaagc gacggctcaa gacatccctg gggaaagtgg
     6961 gcgtgaaaga agagctccta gcagttggca agctcacaca gacatcagga gagactacac
     7021 acacacacac agagccaaca ggagatggta agagcatgaa agcatttatg gagtctccaa
     7081 agcagatctt agactcagca gcaagtctaa ctggcagcaa gaggcagctg agaactccta
     7141 agggaaagtc tgaagtccct gaagacctgg ccggcttcat cgagctcttc cagacaccaa
     7201 gtcacactaa ggaatcaatg actaatgaaa aaactaccaa agtatcctac agagcttcac
     7261 agccagacct agtggacacc ccaacaagct ccaagccaca gcccaagaga agtctcagga
     7321 aagcagacac tgaagaagaa tttttagcat ttaggaaaca aacgccatca gcaggcaaag
     7381 ccatgcacac acccaaacca gcagtaggtg aagagaaaga catcaacacg tttttgggaa
     7441 ctccagtgca gaaactggac cagccaggaa atttacctgg cagcaataga cggctacaaa
     7501 ctcgtaagga aaaggcccag gctctagaag aactgactgg cttcagagag cttttccaga
     7561 caccatgcac tgataacccc acgactgatg agaaaactac caaaaaaata ctctgcaaat
     7621 ctccgcaatc agacccagcg gacaccccaa caaacacaaa gcaacggccc aagagaagcc
     7681 tcaagaaagc agacgtagag gaagaatttt tagcattcag gaaactaaca ccatcagcag
     7741 gcaaagccat gcacacgcct aaagcagcag taggtgaaga gaaagacatc aacacatttg
     7801 tggggactcc agtggagaaa ctggacctgc taggaaattt acctggcagc aagagacggc
     7861 cacaaactcc taaagaaaag gccaaggctc tagaagatct ggctggcttc aaagagctct
     7921 tccagacacc aggtcacact gaggaatcaa tgaccgatga caaaatcaca gaagtatcct
     7981 gcaaatctcc acaaccagac ccagtcaaaa ccccaacaag ctccaagcaa cgactcaaga
     8041 tatccttggg gaaagtaggt gtgaaagaag aggtcctacc agtcggcaag ctcacacaga
     8101 cgtcagggaa gaccacacag acacacagag agacagcagg agatggaaag agcatcaaag
     8161 cgtttaagga atctgcaaag cagatgctgg acccagcaaa ctatggaact gggatggaga
     8221 ggtggccaag aacacctaag gaagaggccc aatcactaga agacctggcc ggcttcaaag
     8281 agctcttcca gacaccagac cacactgagg aatcaacaac tgatgacaaa actaccaaaa
     8341 tagcctgcaa atctccacca ccagaatcaa tggacactcc aacaagcaca aggaggcggc
     8401 ccaaaacacc tttggggaaa agggatatag tggaagagct ctcagccctg aagcagctca
     8461 cacagaccac acacacagac aaagtaccag gagatgagga taaaggcatc aacgtgttca
     8521 gggaaactgc aaaacagaaa ctggacccag cagcaagtgt aactggtagc aagaggcagc
     8581 caagaactcc taagggaaaa gcccaacccc tagaagactt ggctggcttg aaagagctct
     8641 tccagacacc aatatgcact gacaagccca cgactcatga gaaaactacc aaaatagcct
     8701 gcagatctcc acaaccagac ccagtgggta ccccaacaat cttcaagcca cagtccaaga
     8761 gaagtctcag gaaagcagac gtagaggaag aatccttagc actcaggaaa cgaacaccat
     8821 cagtagggaa agctatggac acacccaaac cagcaggagg tgatgagaaa gacatgaaag
     8881 catttatggg aactccagtg cagaaattgg acctgccagg aaatttacct ggcagcaaaa
     8941 gatggccaca aactcctaag gaaaaggccc aggctctaga agacctggct ggcttcaaag
     9001 agctcttcca gacaccaggc actgacaagc ccacgactga tgagaaaact accaaaatag
     9061 cctgcaaatc tccacaacca gacccagtgg acaccccagc aagcacaaag caacggccca
     9121 agagaaacct caggaaagca gacgtagagg aagaattttt agcactcagg aaacgaacac
     9181 catcagcagg caaagccatg gacacaccaa aaccagcagt aagtgatgag aaaaatatca
     9241 acacatttgt ggaaactcca gtgcagaaac tggacctgct aggaaattta cctggcagca
     9301 agagacagcc acagactcct aaggaaaagg ctgaggctct agaggacctg gttggcttca
     9361 aagaactctt ccagacacca ggtcacactg aggaatcaat gactgatgac aaaatcacag
     9421 aagtatcctg taaatctcca cagccagagt cattcaaaac ctcaagaagc tccaagcaaa
     9481 ggctcaagat acccctggtg aaagtggaca tgaaagaaga gcccctagca gtcagcaagc
     9541 tcacacggac atcaggggag actacgcaaa cacacacaga gccaacagga gatagtaaga
     9601 gcatcaaagc gtttaaggag tctccaaagc agatcctgga cccagcagca agtgtaactg
     9661 gtagcaggag gcagctgaga actcgtaagg aaaaggcccg tgctctagaa gacctggttg
     9721 acttcaaaga gctcttctca gcaccaggtc acactgaaga gtcaatgact attgacaaaa
     9781 acacaaaaat tccctgcaaa tctcccccac cagaactaac agacactgcc acgagcacaa
     9841 agagatgccc caagacacgt cccaggaaag aagtaaaaga ggagctctca gcagttgaga
     9901 ggctcacgca aacatcaggg caaagcacac acacacacaa agaaccagca agcggtgatg
     9961 agggcatcaa agtattgaag caacgtgcaa agaagaaacc aaacccagta gaagaggaac
    10021 ccagcaggag aaggccaaga gcacctaagg aaaaggccca acccctggaa gacctggccg
    10081 gcttcacaga gctctctgaa acatcaggtc acactcagga atcactgact gctggcaaag
    10141 ccactaaaat accctgcgaa tctcccccac tagaagtggt agacaccaca gcaagcacaa
    10201 agaggcatct caggacacgt gtgcagaagg tacaagtaaa agaagagcct tcagcagtca
    10261 agttcacaca aacatcaggg gaaaccacgg atgcagacaa agaaccagca ggtgaagata
    10321 aaggcatcaa agcattgaag gaatctgcaa aacagacacc ggctccagca gcaagtgtaa
    10381 ctggcagcag gagacggcca agagcaccca gggaaagtgc ccaagccata gaagacctag
    10441 ctggcttcaa agacccagca gcaggtcaca ctgaagaatc aatgactgat gacaaaacca
    10501 ctaaaatacc ctgcaaatca tcaccagaac tagaagacac cgcaacaagc tcaaagagac
    10561 ggcccaggac acgtgcccag aaagtagaag tgaaggagga gctgttagca gttggcaagc
    10621 tcacacaaac ctcaggggag accacgcaca ccgacaaaga gccggtaggt gagggcaaag
    10681 gcacgaaagc atttaagcaa cctgcaaagc ggaagctgga cgcagaagat gtaattggca
    10741 gcaggagaca gccaagagca cctaaggaaa aggcccaacc cctggaagat ctggccagct
    10801 tccaagagct ctctcaaaca ccaggccaca ctgaggaact ggcaaatggt gctgctgata
    10861 gctttacaag cgctccaaag caaacacctg acagtggaaa acctctaaaa atatccagaa
    10921 gagttcttcg ggcccctaaa gtagaacccg tgggagacgt ggtaagcacc agagaccctg
    10981 taaaatcaca aagcaaaagc aacacttccc tgcccccact gcccttcaag aggggaggtg
    11041 gcaaagatgg aagcgtcacg ggaaccaaga ggttgcgctg catgccagca ccagaggaaa
    11101 ttgtggagga gctgccagcc agcaagaagc agagggttgc tcccagggca agaggcaaat
    11161 catccgaacc cgtggtcatc atgaagagaa gtttgaggac ttctgcaaaa agaattgaac
    11221 ctgcggaaga gctgaacagc aacgacatga aaaccaacaa agaggaacac aaattacaag
    11281 actcggtccc tgaaaataag ggaatatccc tgcgctccag acgccaaaat aagactgagg
    11341 cagaacagca aataactgag gtctttgtat tagcagaaag aatagaaata aacagaaatg
    11401 aaaagaagcc catgaagacc tccccagaga tggacattca gaatccagat gatggagccc
    11461 ggaaacccat acctagagac aaagtcactg agaacaaaag gtgcttgagg tctgctagac
    11521 agaatgagag ctcccagcct aaggtggcag aggagagcgg agggcagaag agtgcgaagg
    11581 ttctcatgca gaatcagaaa gggaaaggag aagcaggaaa ttcagactcc atgtgcctga
    11641 gatcaagaaa gacaaaaagc cagcctgcag caagcacttt ggagagcaaa tctgtgcaga
    11701 gagtaacgcg gagtgtcaag aggtgtgcag aaaatccaaa gaaggctgag gacaatgtgt
    11761 gtgtcaagaa aataagaacc agaagtcata gggacagtga agatatttgg gatcggccgc
    11821 atagataact gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
    11881 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
    11941 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
    12001 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
    12061 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
    12121 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctccctcc
    12181 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
    12241 atgtgatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
    12301 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
    12361 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
    12421 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
    12481 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
    12541 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
    12601 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
    12661 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
    12721 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
    12781 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
    12841 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
    12901 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
    12961 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
    13021 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
    13081 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
    13141 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
    13201 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
    13261 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
    13321 agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
    13381 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc
    13441 gacccgggcg gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagagctcg
    13501 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    13561 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    13621 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    13681 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    13741 ttctgaggcg gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac
    13801 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    13861 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    13921 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    13981 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    14041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    14101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    14161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    14221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    14461 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    14521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    14581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    14641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    14941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    15001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    15061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    15121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    15181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    15241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    15301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    15361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    15421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    15481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    15541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    15601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    15661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    15721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    15781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    15841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    15901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    15961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    16021 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga
    16081 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    16141 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    16201 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
    16261 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    16321 tatggagttc
//
