LOCUS       pODN-mNG-RHOA           5800 bp ds-DNA     circular     09-MAR-2022
DEFINITION  .
KEYWORDS    "creator:Thermo Scientific (Fermentas)" "marker:AmpR"              
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     CDS             complement(171..962)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(963..1031)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1032..1136)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1239..1594)
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        1528..1546
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1551..1594
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1595..2694
                     /label="RHOA left HA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2695..3402
                     /label="Translation 2695-3402"
     misc_feature    2695..3402
                     /label="mNeonGreen"
     misc_feature    3403..3420
                     /label="Linker"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3403..3420
                     /label="Translation 3403-3420"
     misc_feature    3403..3420
                     /label="GGSGGS modified sequence linker"
     CDS             3421..3576
                     /label="Translation 3421-3576"
     misc_feature    3421..4420
                     /label="RHOA right HA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3426..3426
                     /label="T-A mutation"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3429..3429
                     /label="C-T mutation"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3432..3432
                     /label="C-T mutation"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3435..3435
                     /label="G-C mutation"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3438..3438
                     /label="G-A mutation"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4421..4471
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(4421..4802)
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     protein_bind    4831..4847
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(4855..4861)
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(4862..4879)
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(4880..4885)
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(5212..5800)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCGCCCCTG CAGCCGAATT ATATTATTTT TGCCAAATAA
     1261 TTTTTAACAA AAGCTCTGAA GTCTTCTTCA TTTAAATTCT TAGATGATAC TTCATCTGGA
     1321 AAATTGTCCC AATTAGTAGC ATCACGCTGT GAGTAAGTTC TAAACCATTT TTTTATTGTT
     1381 GTATTATCTC TAATCTTACT ACTCGATGAG TTTTCGGTAT TATCTCTATT TTTAACTTGG
     1441 AGCAGGTTCC ATTCATTGTT TTTTTCATCA TAGTGAATAA AATCAACTGC TTTAACACTT
     1501 GTGCCTGAAC ACCATATCCA TCCGGCGTAA TACGACTCAC TATAGGGAGA GCGGCCGCCA
     1561 GATCTTCCGG ATGGCTCGAG TTTTTCAGCA AGATTACCCT GCTTGACAAA TTGtttcctt
     1621 tttttttttt tttttttttg tgagatggag tcttGctctg tcccccaggc tggtgtgcag
     1681 tggcccgatc ttggctcact gcaagctctg tctcctgggt tcacgccatt ctcctgcctc
     1741 agcctcccaa gtagctggga ctacaggtgc ctgccaccat gcccggctag ttatttgtat
     1801 ttttagtaga gatggggttt ctccgtgtta gccaggatgg tctcgatctc ctgacctcgt
     1861 gatccacccg cctcggcctc ccaaagtgct gggattatag gcgtgagcca ctgcgcctgg
     1921 cTcaattgat tttttaatgt taatattgta tcctgcaacc ttactgaact tgtttattct
     1981 aaaagttttc tttaggattt cccacattac aaaatcatct catgtgtgaa ttgagatagt
     2041 tttacttctt cctttccaat ttggatgtct tttatttcat tttcttacct tattgccctg
     2101 gctaggacct ccagtatcct gctggataga agtagcaaga acaggccagg cgtggtggct
     2161 cacgcctgta atcccagcac tttgggaggc tgaggcaggt ggatcacctg aggtcaggag
     2221 ttcaagacca gcctggccaa caaggtgaaa ccccatctct atcaaaaatt agctggatgt
     2281 ggtggtacgt gcctgtaatc ccagctactt gggagtctga ggcaggagaa ttgcttgaac
     2341 ccgggaggct ggaggttgca gtgaactgag attgggccag tgcactccag cctgggcgac
     2401 agagtgagac tccgtctcaa aaaaaaaaag aagtggcaag aacagacatt cttgtcttgt
     2461 tcctgatctt agggggcaga cctttttttc agtcttttac cattaagtat aatgttagtt
     2521 acgagttttt aatgtataac ctttTGGTGC CAGGTGGATC GGCGTACTAG AAGTTTGAAT
     2581 TTGAGTATGC TAATAGAGGT TTATGCCCCA TGGTTACCAA AGCATGTGTC ATCCTGTAAG
     2641 CTACCTATGA CTTCTTGTGC ATTGCAGGTA ATATCTGTGT TTTGTGTTTC AGCAATGGTG
     2701 AGCAAGGGCG AGGAGGATAA CATGGCCTCT CTCCCAGCGA CACATGAGTT ACACATCTTT
     2761 GGCTCCATCA ACGGTGTGGA CTTTGACATG GTGGGTCAGG GCACCGGCAA TCCAAATGAT
     2821 GGTTATGAGG AGTTAAACCT GAAGTCCACC AAGGGTGACC TCCAGTTCTC CCCCTGGATT
     2881 CTGGTCCCTC ATATCGGGTA TGGCTTCCAT CAGTACCTGC CCTACCCTGA CGGGATGTCG
     2941 CCTTTCCAGG CCGCCATGGT AGATGGCTCC GGATACCAAG TCCATCGCAC AATGCAGTTT
     3001 GAAGATGGTG CCTCCCTTAC TGTTAACTAC CGCTACACCT ACGAGGGAAG CCACATCAAA
     3061 GGAGAGGCCC AGGTGAAGGG GACTGGTTTC CCTGCTGACG GTCCTGTGAT GACCAACTCG
     3121 CTGACCGCTG CGGACTGGTG CAGGTCGAAG AAGACTTACC CCAACGACAA AACCATCATC
     3181 AGTACCTTTA AGTGGAGTTA CACCACTGGA AATGGCAAGC GCTACCGGAG CACTGCGCGG
     3241 ACCACCTACA CCTTTGCCAA GCCAATGGCG GCTAACTATC TGAAGAACCA GCCGATGTAC
     3301 GTGTTCCGTA AGACGGAGCT CAAGCACTCC AAGACCGAGC TCAACTTCAA GGAGTGGCAA
     3361 AAGGCCTTTA CCGATGTGAT GGGCATGGAC GAGCTGTACA AGGGAGGTTC AGGAGGCTCC
     3421 ATGGCAGCTA TTCGCAAAAA ACTGGTGATT GTTGGTGATG GAGCCTGTGG AAAGACATGC
     3481 TTGCTCATAG TCTTCAGCAA GGACCAGTTC CCAGAGGTGT ATGTGCCCAC AGTGTTTGAG
     3541 AACTATGTGG CAGATATCGA GGTGGATGGA AAGCAGGTGA GTATACTTTT CATAACAACT
     3601 GATGCCATTT TCCATGTTAG AATATTTATG GAGCATGTAG AGAATTAGAG CTTTTTGCCT
     3661 CTTCAGTATA CCATATAACA AAAATGTTGG AAAGAAGAAT CCATTCTCAT CCTGCCATCT
     3721 TAGTCCAAGt ttgttctttt tttttttttg gagacggagt ctcattctgt tgcccaggct
     3781 agagtgcagt ggctcggtct tggctcactg caacctctgc ctcccaggtt caagtgattc
     3841 tcctgcctca gcctcccaaa tagcagggat tacaggcatg ttgccaccat gctgagctca
     3901 tttttcgttt agtagagatg gggtttcacc aggctggtct caaacttctg acctcgggtg
     3961 atccacccac ctcagcctcc caaagtgctg ggattacagg agtgagccac tgtgcccagc
     4021 cTTCCcccag gtttaaagga ttctcctgcc tcagcctccc cagtagctgg gattacggga
     4081 atatgccacc acacctggct gatttttgta tttttagtag aaatggggtt tcactacgtt
     4141 ggccaggctg gtcatgaact cctgaccgca ggtgatTGGC TGTTTTTTCA TTTTTAACTG
     4201 CTACTCATCC AGACTTTGTT TACATTCATA TGTATTTGAC ATGGTGTCCT TAGCATATGT
     4261 Gttttttttt tgttttgttt tgttgtttga gacagagtct cgctctgtcg cccaggctgg
     4321 agtgcagtgg tacaatctca gctcgctgca acctccacct cccgggttca agcaattctt
     4381 ttgcctcagc ctccagagta gagtagctgg gattacaggc ATCTTTCTAG AAGATCTCCT
     4441 ACAATATTCT CAGCTGCCAT GGAAAATCGA TGTTCTTCTT TTATTCTCTC AAGATTTTCA
     4501 GGCTGTATAT TAAAACTTAT ATTAAGAACT ATGCTAACCA CCTCATCAGG AACCGTTGTA
     4561 GGTGGCGTGG GTTTTCTTGG CAATCGACTC TCATGAAAAC TACGAGCTAA ATATTCAATA
     4621 TGTTCCTCTT GACCAACTTT ATTCTGCATT TTTTTTGAAC GAGGTTTAGA GCAAGCTTCA
     4681 GGAAACTGAG ACAGGAATTT TATTAAAAAT TTAAATTTTG AAGAAAGTTC AGGGTTAATA
     4741 GCATCCATTT TTTGCTTTGC AAGTTCCTCA GCATTCTTAA CAAAAGACGT CTCTTTTGAC
     4801 ATGTTTAAAG TTTAAACCTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACATTAT
     4861 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
     4921 AATTGCGTTG CGCTCACTGC CAATTGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
     4981 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
     5041 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     5101 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     5161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
     5221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     5281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     5341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     5401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     5461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     5521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     5581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     5641 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     5701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     5761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
//