LOCUS       Exported                3454 bp DNA     circular SYN 27-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     /Users/katherineberman/Dropbox(LucksLab)/LucksLab/Group_Members/KEB 
            /Projects/KEB.04.TPP/Experiments/4_001_InvivoCloning/plasmids/KEB02 
            6_TPPThiM_Full_Spe1_sfGFP.apefrom 1 to 3486
            /Users/katherineberman/Downloads/PDC1095 [No hairpincontrol](2).ape 
            from 1 to 3389
FEATURES             Location/Qualifiers
     source          1..3454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..50
                     /locus_tag="Promoter-J23119(SpeI)"
                     /label=Promoter-J23119(SpeI)
     misc_feature    51..192
                     /locus_tag="ThiB TPP Riboswitch E coli"
                     /label=ThiB TPP Riboswitch E coli
     misc_feature    67
                     /locus_tag="Ligand Non-responsive SNP G17C"
                     /label=Ligand Non-responsive SNP G17C
     misc_feature    172..177
                     /locus_tag="Start Codon(1)"
                     /label=Start Codon
                     /label=Start Codon(1)
     misc_feature    181..183
                     /locus_tag="Start Codon"
                     /label=Start Codon
     misc_feature    193..906
                     /locus_tag="SFGFP"
                     /label=SFGFP
     misc_feature    819..824
                     /locus_tag="BamHI-BglII Scar"
                     /label=BamHI-BglII Scar
     misc_feature    complement(910..915)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    916..1283
                     /locus_tag="TrrnB"
                     /label=TrrnB
     misc_feature    complement(952..957)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     misc_feature    1318..2181
                     /locus_tag="CmR(EcoRI-KO) Cassette "
                     /label=CmR(EcoRI-KO) Cassette
                     
     misc_feature    2109..2114
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     misc_feature    2464..3308
                     /locus_tag="p15A oripACYC"
                     /label=p15A oripACYC
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt ccgttctcaa
       61 cggggtccca cgcgtacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg
      121 ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa
      181 gtgttaaaaa aaatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      241 gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat
      301 gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg
      361 tggccaacac ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat
      421 cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc
      481 actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt
      541 gatacccttg ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt
      601 cttggacaca aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa
      661 caaaagaatg gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt
      721 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      781 gacaaccatt acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac
      841 cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc
      901 tacaaataag gatctgaagc ttgggcccga acaaaaactc atctcagaag aggatctgaa
      961 tagcgccgtc gaccatcatc atcatcatca ttgagtttaa acggtctcca gcttggctgt
     1021 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1081 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1141 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1201 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1261 tatctgttgt ttgtcggtga actggatcct tactcgagtc tagactgcag ttgatcgggc
     1321 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     1381 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     1441 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     1501 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     1561 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     1621 ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     1681 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     1741 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     1801 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     1861 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     1921 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     1981 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     2041 aacagtactg cgatgagtgg cagggcgggg cgtaatttga tatcgagctc gcttggactc
     2101 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     2161 tgccgccggg cgttttttat tggtgagaat ccaagcctcc gatcaacgtc tcattttcgc
     2221 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     2281 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     2341 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     2401 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     2461 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     2521 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     2581 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     2641 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     2701 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     2761 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     2821 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     2881 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     2941 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     3001 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     3061 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     3121 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     3181 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     3241 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata
     3301 aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc
     3361 atacgatata agttgtaatt ctcatgtttg acagcttatc atcgataagc ttccgatggc
     3421 gcgccgagag gctttacact ttatgcttcc ggct
//
