LOCUS       Exported                3449 bp DNA     circular SYN 20-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     KEB054_p15a_ThiB_sfGFP from 1 to 3454
            /Users/katherineberman/Dropbox(LucksLab)/LucksLab/Group_Members/KEB 
            /Projects/KEB.04.TPP/Experiments/4_001_InvivoCloning/plasmids/KEB02 
            6_TPPThiM_Full_Spe1_sfGFP.apefrom 1 to 3486
            /Users/katherineberman/Downloads/PDC1095 [No hairpincontrol](2).ape 
            from 1 to 3389
FEATURES             Location/Qualifiers
     source          1..3449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..50
                     /locus_tag="Promoter-J23119(SpeI)"
                     /label=Promoter-J23119(SpeI)
     misc_feature    51..187
                     /locus_tag="ThiB TPP Riboswitch E coli"
                     /label=ThiB TPP Riboswitch E coli
     misc_feature    167..172
                     /locus_tag="Start Codon(1)"
                     /label=Start Codon
                     /label=Start Codon(1)
     misc_feature    176..178
                     /locus_tag="Start Codon"
                     /label=Start Codon
     misc_feature    188..901
                     /locus_tag="SFGFP"
                     /label=SFGFP
     misc_feature    814..819
                     /locus_tag="BamHI-BglII Scar"
                     /label=BamHI-BglII Scar
     misc_feature    complement(905..910)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    911..1278
                     /locus_tag="TrrnB"
                     /label=TrrnB
     misc_feature    complement(947..952)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     misc_feature    1313..2176
                     /locus_tag="CmR(EcoRI-KO) Cassette "
                     /label=CmR(EcoRI-KO) Cassette
                     
     misc_feature    2104..2109
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     misc_feature    2459..3303
                     /locus_tag="p15A oripACYC"
                     /label=p15A oripACYC
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt ccgttctcaa
       61 cggggtgcca cgcgtacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg
      121 ccggcgaagg gatttgaggt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
      181 aaaaaaaatg agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      241 agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac
      301 aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc
      361 aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat
      421 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
      481 atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac
      541 ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg
      601 acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa
      661 gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact
      721 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      781 ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat
      841 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
      901 ataaggatct gaagcttggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg
      961 ccgtcgacca tcatcatcat catcattgag tttaaacggt ctccagcttg gctgttttgg
     1021 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1081 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1141 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1201 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1261 gttgtttgtc ggtgaactgg atccttactc gagtctagac tgcagttgat cgggcacgta
     1321 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     1381 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     1441 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     1501 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     1561 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     1621 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     1681 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     1741 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     1801 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
     1861 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     1921 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     1981 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     2041 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
     2101 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     2161 ccgggcgttt tttattggtg agaatccaag cctccgatca acgtctcatt ttcgccaaaa
     2221 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
     2281 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact
     2341 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     2401 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
     2461 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
     2521 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
     2581 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     2641 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     2701 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     2761 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     2881 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     2941 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     3001 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     3061 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     3121 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     3181 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     3241 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     3301 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     3361 atataagttg taattctcat gtttgacagc ttatcatcga taagcttccg atggcgcgcc
     3421 gagaggcttt acactttatg cttccggct
//
