LOCUS       Exported                3444 bp DNA     circular SYN 20-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     pKEB158_thiB_nucloop_del_p15a from 1 to 3449
            KEB054_p15a_ThiB_sfGFP from 1 to 3454
            /Users/katherineberman/Dropbox(LucksLab)/LucksLab/Group_Members/KEB 
            /Projects/KEB.04.TPP/Experiments/4_001_InvivoCloning/plasmids/KEB02 
            6_TPPThiM_Full_Spe1_sfGFP.apefrom 1 to 3486
            /Users/katherineberman/Downloads/PDC1095 [No hairpincontrol](2).ape 
            from 1 to 3389
FEATURES             Location/Qualifiers
     source          1..3444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..50
                     /locus_tag="Promoter-J23119(SpeI)"
                     /label=Promoter-J23119(SpeI)
     misc_feature    51..182
                     /locus_tag="ThiB TPP Riboswitch E coli"
                     /label=ThiB TPP Riboswitch E coli
     misc_feature    162..167
                     /locus_tag="Start Codon(1)"
                     /label=Start Codon
                     /label=Start Codon(1)
     misc_feature    171..173
                     /locus_tag="Start Codon"
                     /label=Start Codon
     misc_feature    183..896
                     /locus_tag="SFGFP"
                     /label=SFGFP
     misc_feature    809..814
                     /locus_tag="BamHI-BglII Scar"
                     /label=BamHI-BglII Scar
     misc_feature    complement(900..905)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    906..1273
                     /locus_tag="TrrnB"
                     /label=TrrnB
     misc_feature    complement(942..947)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     misc_feature    1308..2171
                     /locus_tag="CmR(EcoRI-KO) Cassette "
                     /label=CmR(EcoRI-KO) Cassette
                     
     misc_feature    2099..2104
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     misc_feature    2454..3298
                     /locus_tag="p15A oripACYC"
                     /label=p15A oripACYC
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt ccgttctcaa
       61 cggggtgcca cgcgtacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg
      121 ccggcgaagg gatttgagga gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa
      181 aaatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      241 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
      301 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
      361 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
      421 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      481 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      541 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      601 aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      661 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
      721 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      781 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
      841 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaataag
      901 gatctgaagc ttgggcccga acaaaaactc atctcagaag aggatctgaa tagcgccgtc
      961 gaccatcatc atcatcatca ttgagtttaa acggtctcca gcttggctgt tttggcggat
     1021 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     1081 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     1141 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     1201 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1261 ttgtcggtga actggatcct tactcgagtc tagactgcag ttgatcgggc acgtaagagg
     1321 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     1381 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     1441 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     1501 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     1561 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     1621 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     1681 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     1741 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     1801 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     1861 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1921 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1981 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     2041 cgatgagtgg cagggcgggg cgtaatttga tatcgagctc gcttggactc ctgttgatag
     2101 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     2161 cgttttttat tggtgagaat ccaagcctcc gatcaacgtc tcattttcgc caaaagttgg
     2221 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     2281 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt
     2341 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
     2401 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag
     2461 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     2521 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     2581 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     2641 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     2701 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     2761 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2821 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2881 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2941 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     3001 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     3061 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     3121 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     3181 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     3241 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     3301 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     3361 agttgtaatt ctcatgtttg acagcttatc atcgataagc ttccgatggc gcgccgagag
     3421 gctttacact ttatgcttcc ggct
//
