LOCUS       pODN-H2BC11-mR2         5703 bp ds-DNA     circular     09-MAR-2022
DEFINITION  .
KEYWORDS    "creator:Thermo Scientific (Fermentas)" "marker:AmpR"              
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        916..921
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        922..939
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        940..946
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    954..970
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             999..1380
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    1330..1380
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1381..2380
                     /label="H2BC11 left HA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2381..2398
                     /label="Linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2381..3112
                     /label="Translation 2381-3112"
     misc_feature    2399..3109
                     /label="mRuby2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3110..4109
                     /label="H2BC11 right HA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3139..3139
                     /label="C-T mutation"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3147..3147
                     /label="G-C mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4110..4153
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4110..4465
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(4158..4176)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        4568..4672
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4673..4741
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             4742..5533
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
      781 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
      841 TCCCGACTGG AAAGCAATTG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
      901 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG GAATTGTGAG
      961 CGGATAACAA TTTCACACAG GAGGTTTAAA CTTTAAACAT GTCAAAAGAG ACGTCTTTTG
     1021 TTAAGAATGC TGAGGAACTT GCAAAGCAAA AAATGGATGC TATTAACCCT GAACTTTCTT
     1081 CAAAATTTAA ATTTTTAATA AAATTCCTGT CTCAGTTTCC TGAAGCTTGC TCTAAACCTC
     1141 GTTCAAAAAA AATGCAGAAT AAAGTTGGTC AAGAGGAACA TATTGAATAT TTAGCTCGTA
     1201 GTTTTCATGA GAGTCGATTG CCAAGAAAAC CCACGCCACC TACAACGGTT CCTGATGAGG
     1261 TGGTTAGCAT AGTTCTTAAT ATAAGTTTTA ATATACAGCC TGAAAATCTT GAGAGAATAA
     1321 AAGAAGAACA TCGATTTTCC ATGGCAGCTG AGAATATTGT AGGAGATCTT CTAGAAAGAT
     1381 ctttgcccag cagcttgttg agctcctcgt cgttgcggat ggccagttgg agatgacgcg
     1441 ggatgatgcg ggtcttcttg ttgtcgcggg ccgcgttgcc cgccagttcc aggatctcgg
     1501 cggtcaggta ctccagcacc gctgccagat acaccggcgc gccggccccg acccgctcgg
     1561 catagttgcc tttgcggagc aggcggtgca ctcggcccac ggggaactgg agaccggccc
     1621 tagaagagcg agtcttggcc ttagcgcggg ctttgcctcc ctgcttgcca cgtccagaca
     1681 tagcgagcgc aactcactac gagcaaccac aaagtgaacg ggaaaggcgg cgctttttat
     1741 aaacactatt gggcgcgaaa aagaagacgt gttgttggtt agggctgcag tttaatttca
     1801 accaatagta gtgcgtcttc tggatttgcg aatcctgatt gggcagacct gacctctgac
     1861 gttaccctga ataactacca atcagacaca agacttcaac tcttcacctt atttgcataa
     1921 gcgattctat ataaaagcgc cttgtcatac cctgctcacg ctgtttttcc ttttcgttgg
     1981 cgctttatag ctacacagtg ctatgccaga gccagcgaag tctgctcccg ccccgaaaaa
     2041 gggctccaag aaggcggtga ctaaggcgca gaagaaagac ggcaagaagc gcaagcgcag
     2101 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     2161 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     2221 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
     2281 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
     2341 cgagggtact aaggccgtca ccaagtacac cagcgctaag gaccctcctg tggccaccat
     2401 ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg
     2461 ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg
     2521 aactcaaacc atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat
     2581 tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc
     2641 tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga
     2701 agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta
     2761 ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaaaac
     2821 caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata
     2881 cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac
     2941 ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca
     3001 ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc
     3061 agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt aaacagtgag
     3121 ttggttgcaa actctcaatc ctaacgcctc ttttaagagc cacccatgtt ctcaaagaaa
     3181 gagctggtgc ttgtattcct cctctgctgg ccactgacaa acccttgtaa cttgctactg
     3241 tgttttttgg tctgaagtag agcagttatt taactaatcc ttagtgactt ttttttttta
     3301 gatctgccat tctaatctta gagttaagta aggagatggg aaattttcta ttataagttc
     3361 gaaaccaatt aaaatacgtt agaaaccaat taaaatactc gtcggtcccc cgtcggttag
     3421 tgatttggaa cagtgccaag ttgcagcggt tgtcagtttg aatttgcccg ggcaacgccc
     3481 gcccttcctc tgcatcctga ttggttgttg tatccaggaa gtccacggtc ggcttgggtg
     3541 cgccaaagga atccaaaccc cgcctttatt tggccttcct tacaaactca gtaaactgtc
     3601 agtttctcat tctttagtat tttatcgctt tcgggaagga gatttatttc taattagttt
     3661 tcgttggata gagcgttttt ttttttaaag ggacccttta tggcaacata atcagttttt
     3721 ttttttttga gaaggagact tcctctgtcg cccagactgg agtgcggtgg cgcgatctca
     3781 gctcactgca gcctccgcct cccgggttcc agcgattctc ctgcttcacc cttccgtgta
     3841 gctgggatta caggcatgcg ccaccatgcc cggataattt ttgtattttt agtagagatg
     3901 gggtttcacc atgttggcta ggctggtctc gaactgactt caggtgatcc actagcctca
     3961 gccttaaagt gctaggatta caggcatgag ccatcaagcc cggctacaac ggaatttgaa
     4021 tgctgtagtt ttcagcctga gaaagatcaa tgctttattt ctgattagaa tactattttc
     4081 cctttatggt atatctatat aagcaaaggA TCTTGCTGAA AAACTCGAGC CATCCGGAAG
     4141 ATCTGGCGGC CGCTCTCCCT ATAGTGAGTC GTATTACGCC GGATGGATAT GGTGTTCAGG
     4201 CACAAGTGTT AAAGCAGTTG ATTTTATTCA CTATGATGAA AAAAACAATG AATGGAACCT
     4261 GCTCCAAGTT AAAAATAGAG ATAATACCGA AAACTCATCG AGTAGTAAGA TTAGAGATAA
     4321 TACAACAATA AAAAAATGGT TTAGAACTTA CTCACAGCGT GATGCTACTA ATTGGGACAA
     4381 TTTTCCAGAT GAAGTATCAT CTAAGAATTT AAATGAAGAA GACTTCAGAG CTTTTGTTAA
     4441 AAATTATTTG GCAAAAATAA TATAATTCGG CTGCAGGGGC GGCCTCGTGA TACGCCTATT
     4501 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
     4561 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     4621 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     4681 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     4741 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     4801 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     4861 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     4921 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     4981 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     5041 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     5101 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     5161 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     5221 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     5281 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     5341 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     5401 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     5461 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     5521 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     5581 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     5641 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     5701 TTC
//