LOCUS       pODN-AAVS1-mR2-CAAX     6729 bp ds-DNA     circular     09-MAR-2022
DEFINITION  .
KEYWORDS    "creator:Thermo Scientific (Fermentas)" "marker:AmpR"              
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        916..921
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        922..939
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        940..946
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    954..970
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             999..1380
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    1330..1380
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1381..2379
                     /label="AAVS1 left HA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2363..2363
                     /label="C-G mutation"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2367..2367
                     /label="C-A mutation"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        2430..2733
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        2734..2937
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3048..3758
                     /label="mRuby2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3048..3857
                     /label="Translation 3048-3857"
     misc_feature    3759..3854
                     /label="CAAX domain"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    3980..4101
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4152..5135)
                     /label="AAVS1 right HA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4164..4164
                     /label="A-C mutation"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4167..4167
                     /label="G-A mutation"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5136..5179
                     /label="MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5136..5491
                     /label="Eco47I/T7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(5184..5202)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        5594..5698
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             5699..5767
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             5768..6559
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
      781 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
      841 TCCCGACTGG AAAGCAATTG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
      901 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG GAATTGTGAG
      961 CGGATAACAA TTTCACACAG GAGGTTTAAA CTTTAAACAT GTCAAAAGAG ACGTCTTTTG
     1021 TTAAGAATGC TGAGGAACTT GCAAAGCAAA AAATGGATGC TATTAACCCT GAACTTTCTT
     1081 CAAAATTTAA ATTTTTAATA AAATTCCTGT CTCAGTTTCC TGAAGCTTGC TCTAAACCTC
     1141 GTTCAAAAAA AATGCAGAAT AAAGTTGGTC AAGAGGAACA TATTGAATAT TTAGCTCGTA
     1201 GTTTTCATGA GAGTCGATTG CCAAGAAAAC CCACGCCACC TACAACGGTT CCTGATGAGG
     1261 TGGTTAGCAT AGTTCTTAAT ATAAGTTTTA ATATACAGCC TGAAAATCTT GAGAGAATAA
     1321 AAGAAGAACA TCGATTTTCC ATGGCAGCTG AGAATATTGT AGGAGATCTT CTAGAAAGAT
     1381 CTCAGTCTGG TCTATCTGCC TGGCCCTGGC CATTGTCACT TTGCGCTGCC CTCCTCTCGC
     1441 CCCGAGTGCC CTTGCTGTGC CGCCGGAACT CTGCCCTCTA ACGCTGCCGT CTCTCTCCTG
     1501 AGTCCGGACC ACTTTGAGCT CTACTGGCTT CTGCGCCGCC TCTGGCCCAC TGCTTCCCCT
     1561 TCCCAGGCAG GTCCTGCTTT CTCTGACCTG CATTCTCTCC CCTGGGCCTG TGCCGCTTTC
     1621 TGTCTGCAGC TTGTGGTCTG GGTCACCTCT ACGGCTGGCC CAGATCCTTC CCTGCCGCAT
     1681 CCTTCAGGTT CCGTCTTCCT CCACTCCCTC TTCCCCTTGC TCTCTGCTGT GTTGCTGCCC
     1741 AAGGATGCTC TTTCCGGAGC ACTTCCTTCT CGGCGCTGCA CCACGTGATG TCCTCTGAGC
     1801 GGATCCTCCC CGTGTCTGGG TCCTCTCCGG GCATCTCTCC TCCCTCACCC AACCCCATGC
     1861 CGTCTTCACT CGCTGGGTTC CCTTTTCCTT CTCCTTCTGG GGCCTGTGCC ATCTCTCGTT
     1921 TCTTAGGATG GCCTTCTCCG ACGGATGTCT CCCTTGCGTC CCGCCTCCCC TTCTTGTAGG
     1981 CCTGCATCAT CACCGTTTTT CTGGACAACC CCAAAGTACC CCGTCTCCCT GGCTTTAGCC
     2041 ACCTCTCCAT CCTCTTGCTT TCTTTGCCTG GACACCCCGT TCTCCTGTGG ATTCGGGTCA
     2101 CCTCTCACCC CTTTCATTTG GGCAGCTCCC CTACCCCCCT TACCTCTCTA GTCTGTGCTA
     2161 GCTCTTCCAG CCCCCTGTCA TGGCATCTTC CAGGGGTCCG AGAGCTCAGC TAGTCTTCTT
     2221 CCTCCAACCC GGGCCCCTAT GTCCACTTCA GGACAGCATG TTTGCTGCCT CCAGGGATCC
     2281 TGTGTCCCCG AGCTGGGACC ACCTTATATT CCCAGGGCCG GTTAATGTGG CTCTGGTTCT
     2341 GGGTACTTTT ATCTGTCCCC TCGACCACAC AGTGGGGCCT AGTAATCAAT TACGGGGTCA
     2401 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
     2461 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
     2521 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
     2581 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
     2641 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
     2701 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT
     2761 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT
     2821 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC
     2881 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTGGT
     2941 TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC AAGCTTCGAA
     3001 TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGT CGCCACCatg gtgtctaagg
     3061 gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
     3121 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
     3181 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
     3241 cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
     3301 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
     3361 tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag
     3421 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaaAacc aagggttggg
     3481 agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
     3541 cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa
     3601 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
     3661 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
     3721 tcgccgggct tggtggtggg atggacgagc tgtacaagtc cggactcaga tctcgagctc
     3781 aagcttcgaa ttcaaagatg agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa
     3841 agtgtgtaat tatgtaaAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT
     3901 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT
     3961 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
     4021 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
     4081 CAAACTCATC AATGTATCTT AAGGCGTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG
     4141 CGTTAAATTT TACTAGGGAC AGGCTTAGTG ACAGAAAAGC CCCATCCTTA GGCCTCCTCC
     4201 TTCCTAGTCT CCTGATATTG GGTCTAACCC CCACCTCCTG TTAGGCAGAT TCCTTATCTG
     4261 GTGACACACC CCCATTTCCT GGAGCCATCT CTCTCCTTGC CAGAACCTCT AAGGTTTGCT
     4321 TACGATGGAG CCAGAGAGGA TCCTGGGAGG GAGAGCTTGG CAgggggtgg gagggaaggg
     4381 ggggaTGCGT GACCTGCCCG GTTCTCAGTG GCCACCCTGC GCTACCCTCT CCCAGAACCT
     4441 GAGCTGCTCT GACGCGGCTG TCTGGTGCGT TTCACTGATC CTGGTGCTGC AGCTTCCTTA
     4501 CACTTCCCAA GAGGAGAAgc agtttggaaa aacaaaatca gaataagttg gtcctgagtt
     4561 ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt
     4621 ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggaGGGG
     4681 GGTGTCCGTG TGGAAAACTC CCTTTGTGAG AATGGTGCGT CCTAGGTGTT CACCAGGTCG
     4741 TGGCCGCCTC TACTCCCTTT CTCTTTCTCC ATCCTTCTTT CCTTAAAGAG TCCCCAGTGC
     4801 TATCTGGGAC ATATTCCTCC GCCCAGAGCA GGGTCCCGCT TCCCTAAGGC CCTGCTCTGG
     4861 GCTTCTGGGT TTGAGTCCTT GGCAAGCCCA GGAGAGGCGC TCAGGCTTCC CTGTCCCCCT
     4921 TCCTCGTCCA CCATCTCATG CCCCTGGCTC TCCTGCCCCT TCCCTACAGG GGTTCCTGGC
     4981 TCTGCTCTTC AGACTGAGcc ccgttcccct gcatccccgt tcccctgcat cccccagagg
     5041 ccccaggCCA CCTACTTGGC CTGGACCCCA CGAGAGGCCA CCCCAGCCCT GTCTACCAGG
     5101 CTGCCTTTTG GGTGGATTCT CCTCCAACTG TGGGGATCTT GCTGAAAAAC TCGAGCCATC
     5161 CGGAAGATCT GGCGGCCGCT CTCCCTATAG TGAGTCGTAT TACGCCGGAT GGATATGGTG
     5221 TTCAGGCACA AGTGTTAAAG CAGTTGATTT TATTCACTAT GATGAAAAAA ACAATGAATG
     5281 GAACCTGCTC CAAGTTAAAA ATAGAGATAA TACCGAAAAC TCATCGAGTA GTAAGATTAG
     5341 AGATAATACA ACAATAAAAA AATGGTTTAG AACTTACTCA CAGCGTGATG CTACTAATTG
     5401 GGACAATTTT CCAGATGAAG TATCATCTAA GAATTTAAAT GAAGAAGACT TCAGAGCTTT
     5461 TGTTAAAAAT TATTTGGCAA AAATAATATA ATTCGGCTGC AGGGGCGGCC TCGTGATACG
     5521 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT
     5581 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
     5641 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
     5701 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
     5761 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
     5821 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
     5881 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
     5941 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
     6001 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
     6061 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
     6121 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
     6181 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
     6241 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
     6301 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
     6361 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
     6421 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
     6481 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
     6541 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
     6601 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
     6661 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     6721 AGGATCTTC
//