LOCUS       Exported                4729 bp ds-DNA     circular SYN 19-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 11, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4444
                     /dnas_title="Exported"
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        279..1659
                     /label=AePUb promoter
     primer_bind     279..308
                     /label=PUbFw-taag
     primer_bind     complement(583..603)
                     /label=EM536
     3'UTR           join(867..1045,1650..1659)
     intron          1046..1649
                     /label=3'UTRintron
     primer_bind     1274..1285
                     /label=EM692
     primer_bind     complement(1627..1659)
                     /label=PUbRv-gttc
     primer_bind     1660..1677
                     /label=EM692
     CDS             1661..2377
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     polyA_signal    2502..2584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2602..2627)
                     /label=EM634
     misc_feature    2622..2701
                     /label=SeqOK
                     /note="SeqOK"
     misc_feature    complement(2622..2653)
                     /label=EM1024
                     /note="EM1024"
     misc_feature    complement(2626..2681)
                     /label=EM1059
                     /note="EM1059"
     misc_feature    2626..2681
                     /label=attB 2
                     /note="attB 2"
     misc_feature    2629..2666
                     /label=minimal attb
                     /note="minimal attb"
     misc_feature    2654..2707
                     /label=EM1025
                     /note="EM1025"
     misc_feature    complement(2705..2723)
                     /label=M13R
                     /note="M13R"
     misc_feature    complement(2749..2767)
                     /label=pDONR-RP
                     /note="pDONR-RP"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggcatccat ctttacatgt agcttgtgca
      301 ttgaatccaa ttataatttg ccttggcacc agctgagcca gacaagaaag aaagcttccc
      361 agaagtatat cgatttagaa gggttgacgt cactttgctg actgcactaa tacagcaaat
      421 gatacaatta gaatgattca agtgaaattc ccaaattact gctttgtctc tggatttggt
      481 tatcagatta cattcgaagc taagaatagc taccgaaatt gtcgatcaaa tcaggaaatc
      541 ctttctctat cgaaaaaggc attcgcacat ctttctctgt atgccatata cacgaatggt
      601 aggtacattg acgtctttgc cagaagttga actgcatcgt tcaaggtaca gaatgaacga
      661 ctaacagaca caagcacgtt ttgctgtcca ttcagacaca gggatggtac ccatagtcga
      721 tcgatttaga gccatccaac cgaacagagg tatatgtatg aatggattgc agaaattttc
      781 tagaagtaca accaccacta cggcagtgtc tataaaacgc ccctgcaaag gcaaaaccag
      841 ctcaatcgaa tacgtttcct agtggagtga acattacgcg gtccaagtaa gcagtgccag
      901 tgcaagtgaa gtgaagtctc tagtgaaaaa gagtgatcca attagccaga ggagaaaatt
      961 tcagagtgaa caaagctttg ttcaaaggac aattactatt aaatttgtga aagtgcattt
     1021 cggtgaaggg aatcttctag tgaaggtagg taaattaaat gatgaaatta tagctatgag
     1081 cgaaaactag tttggtgaat gattcctttg tctttgaatg agcaaactat tttccaagat
     1141 ggcgactatt gagctttgag tgattagtga aaatttgcaa cgcagtttca tcatcattga
     1201 taaaacccaa ttgtgattca cggcgataat catatttcgt tgaatcatcg ctgctaattg
     1261 aattaaattt ctagagcaag cgcgaattcg ccatatttct aaaattaaat attgtggtga
     1321 taattaccca ttaaggtaat attaacacat atcgagaaaa accttgagga aatcgtgaaa
     1381 acttgaagat acgcaatttc caaactacgt agttcaaagt cgaaaacaag ttaatttttc
     1441 actaaaaagt agggcgttgt tgtgacgtca tcaccttcaa gtgtatattt ttcacttggc
     1501 ctgcgactgc aaacgcagac aaagcaaaac aagtttaaaa cctgtcgtgt cgtgctcgaa
     1561 gccaaaggca atgaatcaat atcaaatgag agtttgcatt tcacaaccaa ttactgaagc
     1621 gtttcctcgt ttctttttct gctcaacaga gatttcaaca atggtgagca agggcgagga
     1681 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1741 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1801 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1861 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1921 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1981 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2041 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2101 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2161 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2221 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2281 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2341 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     2401 aaatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2461 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttcttca
     2581 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cgctttgcgg gtgccagggc
     2641 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tatcccctat agtgagtcgt
     2701 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     2761 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     2821 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     2881 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2941 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3001 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3061 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3121 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3181 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3241 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     3301 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3361 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     3421 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3481 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     3541 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     3601 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     3661 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     3721 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3781 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3841 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3901 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3961 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4021 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4081 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4141 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4201 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4261 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4321 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4381 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4441 tgtt
//

