LOCUS       Exported                7924 bp ds-DNA     circular SYN 26-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Li
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 11, 2022 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     4..20
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6..24
                     /label=M13R
                     /note="M13R"
     misc_feature    complement(7..33)
                     /label=EM674
                     /note="EM674"
     misc_feature    19..56
                     /label=EM678
                     /note="EM678"
     misc_feature    complement(61..196)
                     /label=PiggyBac 5'TR
                     /note="PiggyBac 5'TR"
     misc_feature    77..95
                     /label=TR2
                     /note="TR2"
     misc_feature    complement(112..133)
                     /label=AG113
                     /note="AG113"
     misc_feature    complement(125..149)
                     /label=EM67
                     /note="EM67"
     misc_feature    129..146
                     /label=EM25
                     /note="EM25"
     misc_feature    153..176
                     /label=EM68
                     /note="EM68"
     misc_feature    174..196
                     /label=EM69
                     /note="EM69"
     primer_bind     complement(356..379)
                     /label=Rv2 - PiggyBac NO attP NO lox EM835
     misc_feature    complement(371..379)
                     /label=attP'
                     /note="attP'"
     misc_feature    380..382
                     /label=EM191
                     /note="EM191"
     misc_feature    380..382
                     /label=lac promoter
                     /note="lac promoter"
     promoter        387..1767
                     /label=AePUb promoter
     primer_bind     387..416
                     /label=PUbFw-taag
     primer_bind     complement(691..711)
                     /label=EM536
     3'UTR           join(975..1153,1758..1767)
     intron          1154..1757
                     /label=3'UTRintron
     primer_bind     1698..1717
                     /label=EM1203
     primer_bind     complement(1735..1767)
                     /label=PUbRv-gttc
     primer_bind     1768..1791
                     /label=Cre-2A-Fw-AATG
     misc_feature    1769..1816
                     /label=Cre ORF
                     /note="Cre ORF"
     misc_feature    1769..1787
                     /label=EM98
                     /note="EM98"
     CDS             1769..1780
                     /codon_start=1
                     /label=Start
                     /translation="MSGP"
     primer_bind     complement(1778..1780)
                     /label=EM20nn
     CDS             1781..1801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1794..1814)
                     /label=EM104
                     /note="EM104"
     primer_bind     complement(1799..1816)
                     /label=EM2017
     CDS             1817..3631
                     /codon_start=1
                     /label=integrase
                     /note="integrase"
                     /translation="DTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGG
                     RFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPI
                     VSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELG
                     GYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREI
                     KTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILR
                     DPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAW
                     LDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQ
                     HEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERAN
                     LVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEA
                     PKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIE
                     KRASITWAKPPTDDDEDDAQDGTEDVAA"
     primer_bind     1817..1836
                     /label=EM2016
     misc_feature    1817..1834
                     /label=EM100
                     /note="EM100"
     misc_feature    complement(1885..1904)
                     /label=EM145
                     /note="EM145"
     misc_feature    complement(1920..1941)
                     /label=EM144
                     /note="EM144"
     primer_bind     complement(2128..2145)
                     /label=EM2114
     misc_feature    2145
                     /label=C to T mutation in pJM0031
     primer_bind     2146..2163
                     /label=EM2113
     misc_feature    complement(2286..2299)
                     /label=trunc EM144
                     /note="trunc EM144"
     primer_bind     complement(2877..2896)
                     /label=EM2108
     misc_feature    3526..3546
                     /label=EM143
                     /note="EM143"
     misc_feature    complement(3612..3631)
                     /label=EM625
                     /note="EM625"
     primer_bind     complement(3613..3635)
                     /label=EM825
     primer_bind     3632..3658
                     /label=EM820
     misc_feature    3636..3858
                     /label=SV40 term
                     /note="SV40 term"
     polyA_signal    3739..3821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3837..3866)
                     /label=EM1121
     primer_bind     complement(3839..3858)
                     /label=EM634
     primer_bind     3863..3889
                     /label=EM508
     misc_feature    3864..4164
                     /label=18xP3
                     /note="3xP3"
     primer_bind     3882..3905
                     /label=EM508
     protein_bind    3897..3947
                     /label=3x [P3 biding site]
                     /bound_moiety="P3 biinding sites [3x]"
     primer_bind     3914..3940
                     /label=EM508
     primer_bind     3933..3956
                     /label=EM508
     protein_bind    3948..3998
                     /label=3x [P3 biding site]
                     /bound_moiety="P3 biinding sites [3x]"
     primer_bind     3965..3991
                     /label=EM508
     primer_bind     3984..4007
                     /label=EM508
     protein_bind    3999..4049
                     /label=3x [P3 biding site]
                     /bound_moiety="P3 biinding sites [3x]"
     primer_bind     4016..4042
                     /label=EM508
     primer_bind     4035..4058
                     /label=EM508
     protein_bind    4050..4100
                     /label=3x [P3 biding site]
                     /bound_moiety="P3 biinding sites [3x]"
     primer_bind     4067..4093
                     /label=EM508
     primer_bind     4086..4109
                     /label=EM508
     protein_bind    4101..4151
                     /label=3x [P3 biding site]
                     /bound_moiety="P3 biinding sites [3x]"
     primer_bind     4118..4144
                     /label=EM508
     promoter        4165..4374
                     /label=Hsp70 minimal promoter
     misc_feature    4204..4209
                     /label=TATA
                     /note="TATA "
     CDS             4375..5094
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4426..4445)
                     /label=EM901
     primer_bind     complement(4579..4598)
                     /label=EM1576
     primer_bind     complement(4629..4648)
                     /label=AG1006
     primer_bind     4800..4819
                     /label=AG1005
     primer_bind     5116..5135
                     /label=EM820
     polyA_signal    5216..5297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5313..5334)
                     /label=EM1121
     primer_bind     complement(5315..5338)
                     /label=EM634
     misc_feature    complement(5335..5338)
                     /label=EM157
                     /note="EM157"
     protein_bind    5352..5353
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     5353..5382
                     /label=Fw2 - PiggyBac NO attP NO lox EM834
     misc_feature    5367..5462
                     /label=repeated transposase region
                     /note="repeated transposase region"
     misc_feature    complement(5446..5603)
                     /label=original PiggyBac 3'region
                     /note="original PiggyBac 3'region"
     misc_feature    complement(5446..5477)
                     /label=EM71
                     /note="EM71"
     misc_feature    complement(5487..5520)
                     /label=EM72
                     /note="EM72"
     misc_feature    5519..5547
                     /label=EM73
                     /note="EM73"
     misc_feature    5519..5541
                     /label=AG114
                     /note="AG114"
     misc_feature    complement(5541..5559)
                     /label=TR2
                     /note="TR2"
     misc_feature    5544..5570
                     /label=EM70
                     /note="EM70"
     misc_feature    5555..5575
                     /label=direct repeat
                     /note="direct repeat"
     misc_feature    complement(5591..5603)
                     /label=TR1
                     /note="TR1"
     primer_bind     complement(5717..5766)
                     /label=NewBackbone-Rv EM854
     primer_bind     5733..5750
                     /label=EM598
     primer_bind     complement(5733..5749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5733..5749)
                     /label=M13F/AG41
                     /note="M13F/AG41"
     primer_bind     5767..5802
                     /label=Backbone-Fw EM841
     misc_feature    5783..5802
                     /label=EM673
                     /note="EM673"
     primer_bind     5783..5802
                     /label=EM673
     misc_feature    complement(5783..5797)
                     /label=EM675
                     /note="EM675"
     terminator      5914..5941
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(6271..6859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7006..7815)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    7886..7904
                     /label=pDONR-RP
                     /note="pDONR-RP"
ORIGIN
        1 tgccaggaaa cagctatgac catgtaatac gacgatatga tcctgatgca gctagattaa
       61 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
      121 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      181 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
      241 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
      301 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
      361 atagattaga tcgcgctcgc ggatccatct ttacatgtag cttgtgcatt gaatccaatt
      421 ataatttgcc ttggcaccag ctgagccaga caagaaagaa agcttcccag aagtatatcg
      481 atttagaagg gttgacgtca ctttgctgac tgcactaata cagcaaatga tacaattaga
      541 atgattcaag tgaaattccc aaattactgc tttgtctctg gatttggtta tcagattaca
      601 ttcgaagcta agaatagcta ccgaaattgt cgatcaaatc aggaaatcct ttctctatcg
      661 aaaaaggcat tcgcacatct ttctctgtat gccatataca cgaatggtag gtacattgac
      721 gtctttgcca gaagttgaac tgcatcgttc aaggtacaga atgaacgact aacagacaca
      781 agcacgtttt gctgtccatt cagacacagg gatggtaccc atagtcgatc gatttagagc
      841 catccaaccg aacagaggta tatgtatgaa tggattgcag aaattttcta gaagtacaac
      901 caccactacg gcagtgtcta taaaacgccc ctgcaaaggc aaaaccagct caatcgaata
      961 cgtttcctag tggagtgaac attacgcggt ccaagtaagc agtgccagtg caagtgaagt
     1021 gaagtctcta gtgaaaaaga gtgatccaat tagccagagg agaaaatttc agagtgaaca
     1081 aagctttgtt caaaggacaa ttactattaa atttgtgaaa gtgcatttcg gtgaagggaa
     1141 tcttctagtg aaggtaggta aattaaatga tgaaattata gctatgagcg aaaactagtt
     1201 tggtgaatga ttcctttgtc tttgaatgag caaactattt tccaagatgg cgactattga
     1261 gctttgagtg attagtgaaa atttgcaacg cagtttcatc atcattgata aaacccaatt
     1321 gtgattcacg gcgataatca tatttcgttg aatcatcgct gctaattgaa ttaaatttct
     1381 agagcaagcg cgaattcgcc atatttctaa aattaaatat tgtggtgata attacccatt
     1441 aaggtaatat taacacatat cgagaaaaac cttgaggaaa tcgtgaaaac ttgaagatac
     1501 gcaatttcca aactacgtag ttcaaagtcg aaaacaagtt aatttttcac taaaaagtag
     1561 ggcgttgttg tgacgtcatc accttcaagt gtatattttt cacttggcct gcgactgcaa
     1621 acgcagacaa agcaaaacaa gtttaaaacc tgtcgtgtcg tgctcgaagc caaaggcaat
     1681 gaatcaatat caaatgagag tttgcatttc acaaccaatt actgaagcgt ttcctcgttt
     1741 ctttttctgc tcaacagaga tttcaacaat gagcggccct ccaaaaaaga agagaaaggt
     1801 agaagacccg ggcggcgaca cgtacgcggg tgcttacgac cgtcagtcgc gcgagcgcga
     1861 aaattcgagc gcagcaagcc cagcgacaca gcgtagcgcc aacgaagaca aggcggccga
     1921 ccttcagcgc gaagtcgagc gcgacggggg ccggttcagg ttcgtcgggc atttcagcga
     1981 agcgccgggc acgtcggcgt tcgggacggc ggagcgcccg gagttcgaac gcatcctgaa
     2041 cgaatgccgc gccgggcggc tcaacatgat cattgtctat gacgtgtcgc gcttctcgcg
     2101 cctgaaggtc atggacgcga ttccgattgt ctcggaattg ctcgccctgg gcgtgacgat
     2161 tgtttccact caggaaggcg tcttccggca gggaaacgtc atggacctga ttcacctgat
     2221 tatgcggctc gacgcgtcgc acaaagaatc ttcgctgaag tcggcgaaga ttctcgacac
     2281 gaagaacctt cagcgcgaat tgggcgggta cgtcggcggg aaggcgcctt acggcttcga
     2341 gcttgtttcg gagacgaagg agatcacgcg caacggccga atggtcaatg tcgtcatcaa
     2401 caagcttgcg cactcgacca ctccccttac cggacccttc gagttcgagc ccgacgtaat
     2461 ccggtggtgg tggcgtgaga tcaagacgca caaacacctt cccttcaagc cgggcagtca
     2521 agccgccatt cacccgggca gcatcacggg gctttgtaag cgcatggacg ctgacgccgt
     2581 gccgacccgg ggcgagacga ttgggaagaa gaccgcttca agcgcctggg acccggcaac
     2641 cgttatgcga atccttcggg acccgcgtat tgcgggcttc gccgctgagg tgatctacaa
     2701 gaagaagccg gacggcacgc cgaccacgaa gattgagggt taccgcattc agcgcgaccc
     2761 gatcacgctc cggccggtcg agcttgattg cggaccgatc atcgagcccg ctgagtggta
     2821 tgagcttcag gcgtggttgg acggcagggg gcgcggcaag gggctttccc gggggcaagc
     2881 cattctgtcc gccatggaca agctgtactg cgagtgtggc gccgtcatga cttcgaagcg
     2941 cggggaagaa tcgatcaagg actcttaccg ctgccgtcgc cggaaggtgg tcgacccgtc
     3001 cgcacctggg cagcacgaag gcacgtgcaa cgtcagcatg gcggcactcg acaagttcgt
     3061 tgcggaacgc atcttcaaca agatcaggca cgccgaaggc gacgaagaga cgttggcgct
     3121 tctgtgggaa gccgcccgac gcttcggcaa gctcactgag gcgcctgaga agagcggcga
     3181 acgggcgaac cttgttgcgg agcgcgccga cgccctgaac gcccttgaag agctgtacga
     3241 agaccgcgcg gcaggcgcgt acgacggacc cgttggcagg aagcacttcc ggaagcaaca
     3301 ggcagcgctg acgctccggc agcaaggggc ggaagagcgg cttgccgaac ttgaagccgc
     3361 cgaagccccg aagcttcccc ttgaccaatg gttccccgaa gacgccgacg ctgacccgac
     3421 cggccctaag tcgtggtggg ggcgcgcgtc agtagacgac aagcgcgtgt tcgtcgggct
     3481 cttcgtagac aagatcgttg tcacgaagtc gactacgggc agggggcagg gaacgcccat
     3541 cgagaagcgc gcttcgatca cgtgggcgaa gccgccgacc gacgacgacg aagacgacgc
     3601 ccaggacggc acggaagacg tagcggcgta gtaagcataa tcagccatac cacatttgta
     3661 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3721 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3781 agcatcacaa atttcacaaa taaagcattt ttcttcactg cattctagtt gtggtttgtc
     3841 caaactcatc aatgtatcta agtatctaat tcaattagag actaattcaa ttagagtcta
     3901 attcaattag agttatctaa ttcaattaga gactaattca attagagtct aattcaatta
     3961 gagttatcta attcaattag agactaattc aattagagtc taattcaatt agagttatct
     4021 aattcaatta gagactaatt caattagagt ctaattcaat tagagttatc taattcaatt
     4081 agagactaat tcaattagag tctaattcaa ttagagttat ctaattcaat tagagactaa
     4141 ttcaattaga gctaattcaa ttaggatcca agcttatcga tttcgaaccc tcgaccgccg
     4201 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     4261 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     4321 ggggtaccgc tagagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4561 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4801 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4861 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4981 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     5101 cgcgactcta gatcaaatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcgcttgt
     5341 aattcgtcga catgagctca attcgataaa agttttgtta ctttatagaa gaaattttga
     5401 gtttttgttt tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt
     5461 agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg
     5521 atatacagac cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc
     5581 acaatatgat tatctttcta gggttaatct agctgcgtgt tctgcagcgt gtcgagcatc
     5641 ttcatctgct ccatcacgct gtaaaacaca tttgcaccgc gagtctgccc gtcctccacg
     5701 ggttcaaaaa cgtgaatgaa cgaggcgcgc tcactggccg tcgttttaca ggggatgtct
     5761 tcatatatat gaagactccc atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     5821 atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac
     5881 gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt
     5941 ttgcgtttct acaaactctt cctggctagc ggtacgcgta ttaattgcgt tgcgctcact
     6001 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6061 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6901 gaacgacgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt
     6961 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
     7021 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     7081 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     7141 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     7201 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     7261 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     7321 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     7381 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     7441 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     7501 gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     7561 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     7621 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     7681 gggcttccca tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     7741 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg
     7801 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     7861 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca
     7921 gagc
//
