LOCUS       Exported               10398 bp ds-DNA     circular SYN 07-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2022 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    854..1714
                     /label=AmpR
                     /note="Unknown feature type:ORF pUC bla Gene Amp Resist."
     old_sequence    2319..3026
                     /label=flanking sequence
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
     misc_feature    4025..5806
                     /label=GenPart 3-U6-AAEL17774
                     /note="GenPart 3-U6-AAEL17774"
     primer_bind     4048..4065
                     /label=AATTCGAGCTCGCCCGGG
     misc_feature    4080..5062
                     /label=U6_AAEL017774_5,AoSeq
                     /note="U6_AAEL017774_5,AoSeq"
     primer_bind     4080..4097
                     /label=TCATCACAAACGAAACTA
     primer_bind     complement(4560..4577)
                     /label=ATCAAAGGATACAAACTC 1051.S4
     primer_bind     4560..4577
                     /label=GAGTTTGTATCCTTTGAT 1051.S3
     misc_feature    5028..6669
                     /label=GenPart-gRNA1-LS-(U6-1)-3,AoSeq-
                     /note="GenPart-gRNA1-LS-(U6-1)-3,AoSeq-(U6-2)-5,AoSeq-gRNA2
                     "
     primer_bind     complement(5046..5062)
                     /label=CATTTCACTACTCTTGCC
     misc_feature    5063..5082
                     /label=hh target 1
                     /note="hh target 1"
     misc_feature    5083..5168
                     /label=LongScaffold
                     /note="LongScaffold"
     primer_bind     5083..5102
                     /label=GTCTTAGAGCTATGCTGGAA
     misc_feature    5169..5175
                     /label=Terminator
                     /note="Terminator"
     misc_feature    5176..5773
                     /label=U6_AAEL017774_3,AoSeq
                     /note="U6_AAEL017774_3,AoSeq"
     primer_bind     complement(5756..5773)
                     /label=CTCAATACTGGAATCTTG
     misc_feature    5765..7304
                     /label=GenPart 4-U6-AAEL17763
                     /note="GenPart 4-U6-AAEL17763"
     misc_feature    5798..6625
                     /label=U6_AAEL017763_5,AoSeq
                     /note="U6_AAEL017763_5,AoSeq"
     primer_bind     5798..5815
                     /label=ATATGTCTTGCCGAAGCA
     primer_bind     complement(6133..6150)
                     /label=TAATTGGAGCTGCCAGAC 1051.S2
     primer_bind     6133..6150
                     /label=GTCTGGCAGCTCCAATTA 1051.S1
     primer_bind     complement(6606..6625)
                     /label=CATCAATTAGCCTCATTCATC
     misc_feature    6626..6645
                     /label=hh site 2
                     /note="hh site 2"
     misc_feature    6646..6731
                     /label=LongScaffold
                     /note="LongScaffold"
     primer_bind     6646..6665
                     /label=GTCTTAGAGCTATGCTGGAA
     misc_feature    6732..6738
                     /label=Terminator
                     /note="Terminator"
     misc_feature    6739..7248
                     /label=U6_AAEL017763_3,AoSeq
                     /note="U6_AAEL017763_3,AoSeq"
     primer_bind     6739..6756
                     /label=CTAATTGATGTTTAAACAAGATGCTTCGGCAATTA 1051.C3
     primer_bind     6739..6756
                     /label=AAGATGCTTCGGCAATTA
     primer_bind     complement(6743..6753)
                     /label=ATATGTCTTGCCGAAGCA
     primer_bind     complement(7231..7248)
                     /label=CGAAATCGGTTTTGGTGT
     misc_feature    7270..7533
                     /label=3xP3 Promoter
                     /note="3xP3 Promoter"
     primer_bind     7270..7287
                     /label=AATTCGAGCTCGCCCGGG
     CDS             7534..8965
                     /label=tdTomato
     primer_bind     7534..7554
                     /label=ATGGTGAGCAAGGGCGAGGAG
     misc_feature    7555..8211
                     /label=tdTomato-1
                     /note="tdTomato-1"
     misc_feature    8281..8937
                     /label=tdTomato-Codon Optimized for Drosophila
                     /note="tdTomato-Codon Optimized for Drosophila -gene 
                     synthesized"
     primer_bind     8281..8307
                     /label=GTTATCAAGGAATTTATGCGCTTCAAA
     misc_feature    8528..8530
                     /label=Untitled
     primer_bind     complement(8916..8937)
                     /label=GAGGAACAGGTGATGTCGGCCT
     misc_feature    8967..9213
                     /label=Sv40
                     /note="Sv40"
     primer_bind     8973..8999
                     /label=GACTCTAGATCATAATCAGCCATACCA
     primer_bind     complement(9184..9213)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
     misc_binding    9225..9925
                     /label=piggyBacL
     old_sequence    9926..10054
                     /label=flanking seq
     misc_feature    complement(10055..10398)
                     /label=Untitled
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccgggctcct aggagatctt
     4081 catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat atctgtcacc
     4141 agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga cgagttgaaa
     4201 atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga cgacgacgat
     4261 tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat gcaaagcgcg
     4321 ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg acattccacc
     4381 agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg
     4441 tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg gtatttttcg
     4501 gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta attccactag
     4561 agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact
     4621 agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc tccatacatt
     4681 caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa
     4741 attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct ttacagtaag
     4801 cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta gagtatcagc
     4861 aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt tttcgcttct
     4921 ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat gaaatcgccc
     4981 atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga ctatataaga
     5041 gcagaggcaa gagtagtgaa atcaccggcc gagagagtaa aggtcttaga gctatgctgg
     5101 aaacagcata gcaagttaag ataaggctag tccgttatca acttgaaaaa gtggcaccga
     5161 gtcggtgctt tttttggtgg ttgttcatcg attcctaata ttgcagtgaa gcagtttgtt
     5221 gtgcatggcg aagataaggt tagggcgagg aatgccttgg aaataagcgg cactgggccg
     5281 tatgaataaa aagcgttgaa ctttactaca tgtagcatct actcaaaaac aaaatccaca
     5341 aagtttacta cacttttggt atgaataaaa accttttcga acatgtagta cctactactt
     5401 taagaaaatt ctattcaaag tacagagtaa tgctgcatgt taagaaaatt ccattcagag
     5461 tgactcttga aatcaataaa atcatgcata tatgccaaat ttgtcactta ttcacgtgac
     5521 aaattatgtg aaaatatgaa tattactaca ttcccgctat gcatcgcgta atcgatcatg
     5581 aaatttatgt tggttatgta actttacagt attcccttta tgtgcatgtg aattttataa
     5641 tgatttgaca acaaggattt tccttagaat tttcgaattt tagctagaaa tctgtatcct
     5701 atgaatgaat cttgagataa cagcaattgt aacattataa agttcctaac attttcaaga
     5761 ttccagtatt gagtcgcgat cgcttaatta accgcggata tgtcttgccg aagcacatta
     5821 acccaagcca tttatatatc aagtacaagt tgatttgatt ctagtgatgg actgacacga
     5881 atctatggca ctgttgcaga gcttacaaac aggtacccgg agtcgcttca attattcaat
     5941 catccattca aaaacttcca tctcttccaa ttagccaata ttcaaattgg aatccagcca
     6001 gccgacggag gcagatggtc tacctggcaa ctatagagtt tccatgtttc cagactttcc
     6061 ctcccggtaa acggagacaa aacgacagac gtaagtaggt acatatgcat accgcacgga
     6121 caaatcaaat ttgtctggca gctccaatta gagtcgttaa aaatttaacg atgcgctaaa
     6181 taacttcaag ctatttgtct cgctggattg gcttcgagtg gtaagatcct atcaaatgcc
     6241 gaaaacaaaa aactttcttc ttaattgttc gttcttcaac acctctccat ggtgataacg
     6301 gatacggttt cattgtcagc atccatcctc cgaaaaatac attacgcctt gaaatatgca
     6361 atcgcaaaca cggatctgtt tggaacattt attttactat gaagagatgc gataggtaat
     6421 atttatttga gcgtttaaga tactcattgt tctctcaaag aatgtcattg aaagccaacg
     6481 aggtcaaatc aaatattata ataaaaaggt caaagaggac taacttaaag ctctctttat
     6541 ggataggaaa aaatattttc gcccatcgct agaactttta ccgtttccat tgagtatata
     6601 actaagatga atgaggctaa ttgattgggt tccgatatgc agggtgtctt agagctatgc
     6661 tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa aaagtggcac
     6721 cgagtcggtg ctttttttaa gatgcttcgg caattaataa taaggaatgg taaagcattc
     6781 ttcaaatgtt actatgttct caaattttca agaattaggt attcaagaaa ttctttcagg
     6841 catattccat aatctgcaca agcagctact ttagaaatta ttgccgggct taaattatat
     6901 gtacaacatt tcctgagtat tttctgacaa aaattctcac aagggaaggt cacgatggag
     6961 ttacacgggg tttttaaccg gactattttt gttagattct acatgtcgaa atatgaaaaa
     7021 ccccaaagcc tttcgggtga tggaccagtg gtgtagccag gaggggctct gaaaggggta
     7081 attttgtacg tatattatat tattaagcta attggaaatt tgacaaaaat atttttttag
     7141 tatttattgt gatggtagag aacaaaattg acattaatgt acgtttgaaa tgtatttgcc
     7201 atgcctttgg cgtactaaca tggagtatgg acaccaaaac cgatttcggc cttttgggcc
     7261 cggcgcgcca attcgagctc gcccggggat ctaattcaat tagagactaa ttcaattaga
     7321 gctaattcaa ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat
     7381 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     7441 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggggtaccgc
     7501 tagagtcgac ggtacgatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc
     7561 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     7621 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     7681 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     7741 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     7801 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     7861 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     7921 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     7981 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     8041 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     8101 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     8161 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
     8221 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc
     8281 gttatcaagg aatttatgcg cttcaaagtt aggatggagg gatccatgaa cggacatgag
     8341 ttcgagatcg agggagaggg cgagggacgc ccgtatgaag gcacacaaac agccaaactc
     8401 aaggtcacca agggcggacc actgcccttc gcctgggata tcctgagtcc ccagtttatg
     8461 tacggcagca aggcctacgt taagcacccc gctgacatac cggactacaa aaagctgtcc
     8521 tttccggaag gcttcaagtg ggagcgcgtg atgaatttcg aagacggagg actggtcact
     8581 gtgacccaag atagcagttt gcaggacggt acactgatct ataaggttaa aatgcgcggc
     8641 actaactttc cgccagatgg cccagtgatg cagaagaaga ccatgggttg ggaggcatcc
     8701 accgaacgtc tgtaccctcg agacggagtg ctcaagggcg agatccatca ggccctcaaa
     8761 ctgaaagatg gtggtcacta cctggtcgaa tttaagacca tttacatggc caagaagccg
     8821 gttcagctgc ccggatatta ttatgtggat acgaaactgg atataacttc gcataacgaa
     8881 gactacacca ttgtcgagca gtatgagcgc agcgaaggcc gacatcacct gttcctctac
     8941 ggcatggacg agctgtacaa gtaggcggcc gcgactctag atcataatca gccataccac
     9001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     9061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     9121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9181 tttgtccaaa ctcatcaatg tatcttaaag cttctaggcc ggccgatctc ggatctgaca
     9241 atgttcagtg cagagactcg gctacgcctc gtggactttg aagttgacca acaatgttta
     9301 ttcttacctc taatagtcct ctgtggcaag gtcaagattc tgttagaagc caatgaagaa
     9361 cctggttgtt caataacatt ttgttcgtct aatatttcac taccgcttga cgttggctgc
     9421 acttcatgta cctcatctat aaacgcttct tctgtatcgc tctggacgtc atcttcactt
     9481 acgtgatctg atatttcact gtcagaatcc tcaccaacaa gctcgtcatc gctttgcaga
     9541 agagcagaga ggatatgctc atcgtctaaa gaactaccca ttttattata tattagtcac
     9601 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
     9661 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
     9721 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
     9781 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
     9841 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
     9901 tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa
     9961 tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa
    10021 tggcaaacca agtcgcgcga gcgtcgactc tagaggatcc ccgggtaccg agctcgaatt
    10081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10261 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10381 caaaggcggt aatacggt
//
