LOCUS       Exported               10179 bp DNA     circular SYN 18-FEB-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Jin Zhang Lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 18, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10179
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     regulatory      905..914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             907..2172
                     /codon_start=1
                     /label=LAMP1
                     /note="note=""direct strand 
                     translation""translation=""KLAAATMAAPGSARRPLLLLLLLLLLGLMHCA
                     SAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENT
                     SDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITD
                     IRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPP
                     APPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNK
                     TSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAA
                     NGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDEN
                     SMLIPIAVGGALAGLVLIVLIAYLVGRKRSHAGYQTIGGGGG"""
                     /note="color: #800040"
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFAYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTPHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTIGGGGG"
     CDS             2182..4953
                     /codon_start=1
                     /label=INPP4B
                     /note="color: #0080c0"
                     /translation="MEIKEEGASEEGQHFLPTAQANDPGDCQFTSIQKTPNEPQLEFIL
                     ACKDLVAPVRDRKLNTLVQISVIHPVEQSLTRYSSTEIVEGTRDPLFLTGVTFPSEYPI
                     YEETKIKLTVYDVKDKSHDTVRTSVLPEHKDPPPEVGRSFLGYASFKVGELLKSKEQLL
                     VLSLRTSDGGKVVGTIEVSVVKMGEIEDGEADHITTDVQGQKCALVCECTAPESVSGKD
                     NLPFLNSVLKNPVCKLYRFPTSDNKWMRIREQMSESILSFHIPKELISLHIKEDLCRNQ
                     EIKELGELSPHWDNLRKNVLTHCDQMVNMYQDILTELSKETGSSFKSSSSKGEKTLEFV
                     PINLHLQRMQVHSPHLKDALYDVITVGAPAAHFQGFKNGGLRKLLHRFETERRNTGYQF
                     IYYSPENTAKAKEVLSNINQLQPLIATHADLLLNSASQHSPDSLKNSLKMLSEKTELFV
                     HAFKDQLVRSALLALYTARPGGILKKPPSPKSSTEESSPQDQPPVMRGQDSIPHHSDYD
                     EEEWDRVWANVGKSLNCIIAMVDKLIERDGGSEGSGGNNDGEKEPSLTDAIPSHPREDW
                     YEQLYPLILTLKDCMGEVVNRAKQSLTFVLLQELAYSLPQCLMLTLRRDIVFSQALAGL
                     VCGFIIKLQTSLYDPGFLQQLHTVGLIVQYEGLLSTYSDEIGMLEDMAVGISDLKKVAF
                     KIIEAKSNDVLPVITGRREHYVVEVKLPARMFESLPLQIKEGQLLHVYPVLFNVGINEQ
                     QTLAERFGDVSLQESINQENFELLQEYYKIFMEKMPPDYISHFQEQNDLKALLENLLQN
                     IQSKKRKNVEIMWLAATICRKLNGIRFTCCKSAKDRTSMSVTLEQCSILRDEHQLHKDF
                     FIRALDCMRREGCRIENVLKNIKCRKYAFNMLQLMAFPKYYRPPEGTYGKADT"
     CDS             4960..5670
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        complement(5731..5749)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    5775..5999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6045..6473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6487..6817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6668..6803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6884..7678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7852..7973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8022..8038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8046..8062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8070..8100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8070 .. 8076 / #ffffff / -10
                        2: 8077 .. 8094 / #ffffff
                        3: 8095 .. 8100 / #ffffff / -35"
     protein_bind    8115..8136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8424..9012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9183..10043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9183 ..  9974 / #ccffcc
                        2:  9975 .. 10043 / #ccffcc / signal sequence
                     Cleavage site after base 9974"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10044..10148)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg cggcgcccgg cagcgcccgg cgacccctgc tgctgctact gctgttgctg
      961 ctgctcggcc tcatgcattg tgcgtcagca gcaatgttta tggtgaaaaa tggcaacggg
     1021 accgcgtgca taatggccaa cttctctgct gccttctcag tgaactacga caccaagagt
     1081 ggccctaaga acatgacctt tgacctgcca tcagatgcca cagtggtgct caaccgcagc
     1141 tcctgtggaa aagagaacac ttctgacccc agtctcgtga ttgcttttgg aagaggacat
     1201 acactcactc tcaatttcac gagaaatgca acacgttaca gcgtccagct catgagtttt
     1261 gcttataact tgtcagacac acaccttttc cccaatgcga gctccaaaga aatcaagact
     1321 gtggaatcta taactgacat cagggcagat atagataaaa aatacagatg tgttagtggc
     1381 acccaggtcc acatgaacaa cgtgaccgta acgccccatg atgccaccat ccaggcgtac
     1441 ctttccaaca gcagcttcag caggggagag acacgctgtg aacaagacag gccttcccca
     1501 accacagcgc cccctgcgcc acccagcccc tcgccctcac ccgtgcccaa gagcccctct
     1561 gtggacaagt acaacgtgag cggcaccaac gggacctgcc tgctggccag catggggctg
     1621 cagctgaacc tcacctatga gaggaaggac aacacgacgg tgacaaggct tctcaacatc
     1681 aaccccaaca agacctcggc cagcgggagc tgcggcgccc acctggtgac tctggagctg
     1741 cacagcgagg gcaccaccgt cctgctcttc cagttcggga tgaatgcaag ttctagccgg
     1801 tttttcctac aaggaatcca gttgaataca attcttcctg acgccagaga ccctgccttt
     1861 aaagctgcca acggctccct gcgagcgctg caggccacag tcggcaattc ctacaagtgc
     1921 aacgcggagg agcacgtccg tgtcacgaag gcgttttcag tcaatatatt caaagtgtgg
     1981 gtccaggctt tcaaggtgga aggtggccag tttggctctg tggaggagtg tctgctggac
     2041 gagaacagca tgctgatccc catcgctgtg ggtggtgccc tggcggggct ggtcctcatc
     2101 gtcctcatcg cctacctcgt cggcaggaag aggagtcacg caggctacca gactatcggt
     2161 ggtggcggtg gtgcggatcc catggaaatt aaagaggaag gggcatcaga agaagggcag
     2221 cactttcttc ctacagccca ggccaatgat cccggggact gtcagttcac aagtatccag
     2281 aagactccaa atgaaccgca gttggaattc atccttgcat gcaaggatct cgtggctcct
     2341 gtccgtgatc gtaaactgaa tacactggtg cagatctccg taatccaccc cgtggagcag
     2401 agtctgacaa gatactccag caccgaaatt gtggagggaa caagggaccc actgtttttg
     2461 actggtgtca cattcccatc tgagtatccc atctatgagg agaccaaaat aaaactaaca
     2521 gtctatgatg tcaaggataa gtctcatgac accgttcgaa ccagtgtcct accagaacat
     2581 aaggatcccc cgccagaagt tgggcgaagt ttcttgggct atgccagttt taaagtggga
     2641 gagctgctga agtcaaagga gcaattgctg gtcctgagcc tgagaacttc agatggtggc
     2701 aaagtggttg gcaccataga agtcagtgtc gtgaagatgg gggagattga ggatggggaa
     2761 gccgaccaca tcaccacaga tgtacaggga caaaagtgtg ccctggtatg tgaatgtaca
     2821 gccccggaaa gtgtgagcgg aaaagataac ttaccttttt tgaattcagt gttaaagaac
     2881 ccagtatgta aattatatag atttcccaca tctgacaata agtggatgcg aattcgagag
     2941 cagatgtcag agagcattct ttcctttcat attcctaagg aattgatttc ccttcacatt
     3001 aaagaagatt tgtgcagaaa ccaggagata aaagaacttg gtgagctttc tccacattgg
     3061 gacaatctgc gaaaaaatgt ccttactcac tgtgatcaaa tggtgaatat gtaccaagac
     3121 attctgacag aacttagcaa ggaaacaggg tcctctttca aatcaagcag cagcaaagga
     3181 gagaaaacat tagaatttgt tccaataaat ctacatctgc aaagaatgca ggtacacagc
     3241 cctcacttga aagatgctct ctacgatgtc atcactgtgg gagccccagc tgcccatttt
     3301 cagggattta agaatggtgg tcttcggaag ctactccata gatttgaaac agaaagaaga
     3361 aataccggat accagtttat ttactattca cctgaaaaca cagccaaagc aaaggaagtt
     3421 ctcagcaaca tcaatcaact acaacctctt atagcaaccc atgcagacct actgcttaat
     3481 tctgcaagcc agcattctcc agacagcttg aagaattctt taaagatgct ttcagaaaaa
     3541 acagagcttt ttgtacatgc cttcaaggat caacttgtca ggagtgctct tttagcactc
     3601 tacactgcaa ggccaggagg cattcttaag aagccaccct ctcctaagag cagcacagag
     3661 gagagcagtc cccaagacca acccccagtg atgagagggc aggactccat accacatcat
     3721 tcagactatg atgaggaaga gtgggacagg gtgtgggcca atgtggggaa gagcctgaac
     3781 tgcattattg ctatggtgga caaactgatt gaaagagatg gtggcagtga aggcagtggc
     3841 ggcaacaatg atggagaaaa ggaaccttca ttaacagatg ccattccctc tcacccaaga
     3901 gaggactggt atgaacagtt gtatcccctc atccttaccc tgaaggactg catgggagaa
     3961 gtggtgaacc gagccaagca gtccctgaca tttgtgctcc ttcaggaact tgcgtacagc
     4021 ttgccccagt gtctgatgct gacgctaaga agagacatcg tcttcagcca agcacttgct
     4081 ggattggttt gtggttttat catcaaatta cagacaagtc tgtatgaccc aggcttccta
     4141 cagcagcttc acacagtggg gttgatagta caatatgaag gactgctaag tacatacagc
     4201 gatgaaattg gaatgctaga ggacatggcc gttggcattt ccgatttaaa gaaagttgca
     4261 tttaaaataa ttgaagccaa atccaatgat gtgttgccag ttataacagg aagacgagaa
     4321 cattacgtgg tagaggtcaa gcttccagcc agaatgtttg agtcactacc tctacagatt
     4381 aaagaaggac agttgcttca tgtgtatcca gtacttttta atgttggaat caatgaacag
     4441 caaactctgg ctgaaaggtt tggagatgtc tctttgcaag aaagtattaa tcaggaaaac
     4501 ttcgaacttc tacaagaata ttacaagata tttatggaaa agatgcctcc tgattatatt
     4561 tcacattttc aggaacaaaa tgatttaaaa gcattgctag aaaatctcct tcaaaatatc
     4621 caatccaaaa aaagaaagaa tgtagaaatt atgtggctgg ctgcaacgat ttgccgcaaa
     4681 ctgaatggta ttcgtttcac ctgttgtaaa agtgccaaag acaggacatc gatgtcagtg
     4741 acacttgaac aatgctcaat cttgagagat gagcaccagt tacacaagga cttctttatc
     4801 cgagcgctgg attgcatgag aagagaagga tgccgcatag agaatgtact gaagaatatc
     4861 aaatgcagaa agtatgcttt caacatgcta cagctgatgg ctttccccaa gtactacaga
     4921 cctccagagg ggacttatgg aaaagctgac accgaattca tggtgagcaa gggcgaggag
     4981 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     5041 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5101 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     5161 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     5221 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5281 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     5341 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     5401 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     5461 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     5521 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     5581 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     5641 accggcggca tggacgagct gtacaagtaa gaattctgca gatatccatc acactggcgg
     5701 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     5761 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5881 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5941 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6001 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6061 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6121 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6181 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     6241 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6301 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6361 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     6421 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     6481 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
     6541 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6601 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6661 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6721 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6781 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6841 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6901 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6961 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7021 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7081 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7141 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7201 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7261 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7321 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7381 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7441 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7501 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7561 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7621 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7681 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     7741 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7801 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7981 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8161 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8221 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8581 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     8641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8821 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10141 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
