LOCUS       _v1_CMV.ifna-10xH-Mch-S 4832 bp ds-DNA     circular     25-MAR-2022
DEFINITION  .
KEYWORDS    "creator:Nick SnapGene" "marker:HygR, OH-R"                        
FEATURES             Location/Qualifiers
     misc_feature    181..184
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    185..215
                     /label="CMV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    253..255
                     /label="M"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    253..1596
                     /label="mNTERY00"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             253..1596
                     /label="Translation 253-1596"
     Signal Peptide  256..321
                     /label="NLS > IFNa2 "
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    325..354
                     /label="10xHis"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             355..1062
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     Polymorphism    1063..1356
                     /label="SUMOStar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1357..1596
                     /label="pore_tail"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     polyA_signal    1626..1840
                     /label="BGH polyA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2096..2194
                     /label="bla promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2195..3055
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3200..3873
                     /label="pUC ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        join(4393..4832,1..180)
                     /label="CMV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT
       61 TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA
      121 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT CTCTGGCTAA
      181 CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC GACTCACTAT AGGGAGACCC
      241 AAGCTGGCTA GCATGGCCCT TACGTTTGCA CTGCTCGTTG CGTTGCTGGT CCTGAGCTGC
      301 AAGTCCAGTT GCTCCGTAGG TgggCACCAT CACCACCACC ACCATCACCA CCATGTGAGC
      361 AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG
      421 GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC
      481 GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
      541 GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC
      601 ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC
      661 TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC
      721 ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG
      781 AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG
      841 GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG
      901 ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG
      961 TTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG
     1021 GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGT CTGGTAGCCT GCAAGATTCG
     1081 GAAGTCAACC AAGAAGCAAA GCCGGAAGTC AAGCCGGAAG TGAAACCGGA AACCCATATT
     1141 AACCTGAAAG TTAGTGACGG CAGCTCTGAA ATTTTCTTTA AGATCAAAAA GACCACGCCG
     1201 CTGCGTCGCC TGATGGAAGC GTTTGCCAAA CGTCAGGGCA AGGAAATGGA TAGCCTGacg
     1261 TTCCTGTATG ACGGTATTga aATCCAGGCA GATCAAGCGC CGGAAGACCT GGACATGGAA
     1321 GACAACGACA TCATTGAAGC CCACCGTGAA CAGATTGGTG GTGGTGGTTC GAGCGGCGGG
     1381 AGCGGAGGGT CTGGGTCTAG CGGGGATGGT GGGTCGTCTG GGGGGAGCGG CGGTTCGGGT
     1441 TCATCTGGTG ACGGCGGTTC CTCGGGTGGT TCCGGGGGAG ATGGTTCTTC CGGAGATGGC
     1501 GGGAGTGATG GGGACTCTGA TGGTTCGGAT GGTGACGGGG ACAGTGATGG TGATGACGGA
     1561 GGGGACGACG AGGATGACGG TTCAGACGAT TAATGAGGCG GCTCGAGGAT CCGGCTGCTA
     1621 ACAAAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
     1681 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
     1741 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
     1801 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CGACGGTGCT CGAAGCAGCG
     1861 CAAAACGCCT AACCCTAAGC AGATTCTTCA TGCAATTGTC GGTCAAGCCT TGCCTTGTTG
     1921 TAGCTTAAAT TTTGCTCGCG CACTACTCAG CGACCTCCAA CACACAAGCA GGGAGCAGAT
     1981 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AGGGGATCGG
     2041 GAGATCTCCC GATCCGTCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     2101 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     2161 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     2221 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     2281 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     2341 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     2401 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     2461 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     2521 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     2581 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     2641 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     2701 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     2761 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     2821 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     2881 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC
     2941 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     3001 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     3061 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     3121 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     3181 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     3241 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     3301 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     3361 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     3421 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     3481 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     3541 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     3601 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     3661 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     3721 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     3781 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     3841 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
     3901 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
     3961 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT
     4021 CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     4081 GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT
     4141 ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC
     4201 AGGAAACAGC TATGACCATG ATTACGCCAA GCTCTAGCTA GAGGTCGACG GTATATGCCC
     4261 ACTGACGGGC ACCGGAGCCT CACGAGGCAC TCCTTTCAAG ACCTAGAAGG TCCATTAGCT
     4321 GCAAAGATTC CTCTCTGTTT AAAACTTTAT CCATCTTTGC AAAGCTTTTT GCAAAAGCCT
     4381 CCCACTGCTT CGCGATGTAC GGGCCAGATA TACGCGTTGA CATTGATTAT TGACTAGTTA
     4441 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
     4501 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
     4561 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
     4621 GGACTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
     4681 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
     4741 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT
     4801 GATGCGGTTT TGGCAGTACA TCAATGGGCG TG
//