LOCUS       pMC_mNG2(11)_BSDminus_F 5168 bp ds-DNA     circular     16-MAR-2022
DEFINITION  .
KEYWORDS    "NHEJ Tagging"                                                     
FEATURES             Location/Qualifiers
     misc_feature    829..848
                     /label="gRNA_1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    831..832
                     /label="cut_1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    877..878
                     /label="L1ext - insCT - fs"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    879..923
                     /label="L1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    924..971
                     /label="mNG2-11"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             924..971
                     /label="Translation 877-1014"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            924..971
                     /label="Translation 924-971"
     misc_feature    972..1016
                     /label="L3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1017..1017
                     /label="L3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1017..1017
                     /label="L3ext - insG"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     polyA_signal    1030..1111
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1030..1623)
                     /label="BSD and SV40"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1624..1882)
                     /label="EF1a core promoter and start"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1884..1903
                     /label="gRNA_1 rev"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1900..1901
                     /label="cut_2"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2039..2058
                     /label="P0460 - rv seq primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ACATTACCCT AGGTTATCCC TAGATACATT ACCCTAGGTT ATCCCAGATG ACATACCCTG
       61 TTATCCCTAG ATGACATTAC CCTGTTATCC CAGATGACAT TACCCTGTTA TCCCTAGATA
      121 CATTACCCTG TTATCCCAGA TGACATACCC TGTTATCCCT AGATGACATT ACCCTGTTAT
      181 CCCAGATGAC ATTACCCTGT TATCCCTAGA TACATTACCC TGTTATCCCA GATGACATAC
      241 CCTGTTATCC CTAGATGACA TTACCCTGTT ATCCCAGATG ACATTACCCT GTTATCCCTA
      301 GATACATTAC CCTGTTATCC CAGATGACAT ACCCTGTTAT CCCTAGATGA CATTACCCTG
      361 TTATCCCAGA TGACATTACC CTGTTATCCC TAGATACATT ACCCTGTTAT CCCAGATGAC
      421 ATACCCTGTT ATCCCTAGAT GACATTACCC TGTTATCCCA GATGACATTA CCCTGTTATC
      481 CCTAGATACA TTACCCTGTT ATCCCAGATG ACATACCCTG TTATCCCTAG ATGACATTAC
      541 CCTGTTATCC CAGATGACAT TACCCTGTTA TCCCTAGATA CATTACCCTG TTATCCCAGA
      601 TGACATACCC TGTTATCCCT AGATGACATT ACCCTGTTAT CCCAGATGAC ATTACCCTGT
      661 TATCCCTAGA TACATTACCC TGTTATCCCA GATGACATAC CCTGTTATCC CTAGATGACA
      721 TTACCCTGTT ATCCCAGATA AACTCAATGA TGATGATGAT GATGGTCGAG ACTCAGCGGC
      781 CGCGGTGCCA GGGCGTGCCC TTGGGCTCCC CGGGCGCGAC TAGTGcctca cgaaatcgat
      841 tcgctcttCT AATCTCCTCT CTTCTCCTCT CTCCAGCTGG TGGCTCTGGA AGTTCAGGTG
      901 GAGGCTCGGG TGGCGGCAGT TCGACCGAGC TCAACTTCAA GGAGTGGCAA AAGGCCTTTA
      961 CCGATATGAT GGGATCCGGA AGTGGCTCAA GCGGAGGAGG AAGTAGTGGA AGTTCTGGTA
     1021 AGTATTGGTg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     1141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     1201 tatggctgat tatgatcctc tggagattta gccctcccac acataaccag agggcagcaa
     1261 ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc
     1321 aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt
     1381 ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc
     1441 cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa
     1501 gatgcggccg tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat
     1561 gctgttgatt gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt
     1621 ggccatggtg gcggatcccg cgtcacgaca cctgtgttct ggcggcaaac ccgttgcgaa
     1681 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     1741 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     1801 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     1861 cccactgacg ggcaccggag ccTaagagcg aatcgatttc gtgaggCGCC CCAACTGGGG
     1921 TAACCTTTGA GTTCTCTCAG TTGGGGGTAA TCAGCATCAT GATGTGGTAC CACATCATGA
     1981 TGCTGATTAT AAGAATGCGG CCGCCACACT CTAGTGGATC TCGAGTTAAT AATTCAGAAG
     2041 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA
     2101 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC
     2161 AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA
     2221 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA
     2281 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC
     2341 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT
     2401 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC
     2461 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC
     2521 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA
     2581 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC
     2641 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC
     2701 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG
     2761 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT
     2821 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCAG AGCTTGATCC CCTGCGCCAT
     2881 CAGATCCTTG GCGGCGAGAA AGCCATCCAG TTTACTTTGC AGGGCTTCCC AACCTTACCA
     2941 GAGGGCGCCC CAGCTGGCAA TTCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC
     3001 TATCGCCATG TAAGCCCACT GCAAGCTACC TGCTTTCTCT TTGCGCTTGC GTTTTCCCTT
     3061 GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT
     3121 TTCTACGTGC TCGAGgggGg ccAAACGGTC TCCAGCTTGG CTGTTTTGGC GGATGAGAGA
     3181 AGATTTTCAG CCTGATACAG ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT
     3241 TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC
     3301 GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT
     3361 CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG
     3421 GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA
     3481 CGGCCCGGAG GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG
     3541 AAGGCCATCC TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA
     3601 AATACATTCA AATATGTATC CGCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
     3661 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
     3721 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
     3781 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
     3841 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
     3901 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
     3961 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
     4021 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
     4081 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
     4141 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
     4201 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
     4261 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
     4321 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
     4381 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
     4441 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
     4501 TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC
     4561 ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA
     4621 CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC
     4681 AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
     4741 AAACGCGCGA GGCAGCAGAT CAATTCGCGC GCGAAGGCGA AGCGGCATGC ATAATGTGCC
     4801 TGTCAAATGG ACGAAGCAGG GATTCTGCAA ACCCTATGCT ACTCCGTCAA GCCGTCAATT
     4861 GTCTGATTCG TTACCAATTA TGACAACTTG ACGGCTACAT CATTCACTTT TTCTTCACAA
     4921 CCGGCACGGA ACTCGCTCGG GCTGGCCCCG GTGCATTTTT TAAATACCCG CGAGAAATAG
     4981 AGTTGATCGT CAAAACCAAC ATTGCGACCG ACGGTGGCGA TAGGCATCCG GGTGGTGCTC
     5041 AAAAGCAGCT TCGCCTGGCT GATACGTTGG TCCTCGCGCC AGCTTAAGAC GCTAATCCCT
     5101 AACTGCTGGC GGAAAAGATG TGACAGACGC GACGGCGACA AGCAAACATG CTGTGCGACG
     5161 CTGGCGAT
//