LOCUS       pMC_mClover3_BSD        5831 bp    DNA        circular     16-MAR-2022
DEFINITION  .
KEYWORDS    "NHEJ Tagging"                                                     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    829..848
                     /locus_tag="gRNA_1"
                     /label="gRNA_1"
                     /ApEinfo_label="gRNA_1"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    877..921
                     /locus_tag="L1"
                     /label="L1"
                     /ApEinfo_label="L1"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    922..1635
                     /locus_tag="mClover3"
                     /label="mClover3"
                     /ApEinfo_label="mClover3"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1636..1680
                     /locus_tag="L3"
                     /label="L3"
                     /ApEinfo_label="L3"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    1693..1774
                     /locus_tag="SV40 poly(A) signal"
                     /label="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1693..2286)
                     /locus_tag="BSD and SV40"
                     /label="BSD and SV40"
                     /ApEinfo_label="BSD and SV40"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2287..2545)
                     /locus_tag="EF1a core promoter and start"
                     /label="EF1a core promoter and start"
                     /ApEinfo_label="EF1a core promoter and start"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2547..2566
                     /locus_tag="gRNA_1-2"
                     /label="gRNA_1-2"
                     /ApEinfo_label="gRNA_1-2"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2702..2721
                     /locus_tag="P0460 - rv seq primer"
                     /label="P0460 - rv seq primer"
                     /ApEinfo_label="P0460 - rv seq primer"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ACATTACCCT AGGTTATCCC TAGATACATT ACCCTAGGTT ATCCCAGATG ACATACCCTG
       61 TTATCCCTAG ATGACATTAC CCTGTTATCC CAGATGACAT TACCCTGTTA TCCCTAGATA
      121 CATTACCCTG TTATCCCAGA TGACATACCC TGTTATCCCT AGATGACATT ACCCTGTTAT
      181 CCCAGATGAC ATTACCCTGT TATCCCTAGA TACATTACCC TGTTATCCCA GATGACATAC
      241 CCTGTTATCC CTAGATGACA TTACCCTGTT ATCCCAGATG ACATTACCCT GTTATCCCTA
      301 GATACATTAC CCTGTTATCC CAGATGACAT ACCCTGTTAT CCCTAGATGA CATTACCCTG
      361 TTATCCCAGA TGACATTACC CTGTTATCCC TAGATACATT ACCCTGTTAT CCCAGATGAC
      421 ATACCCTGTT ATCCCTAGAT GACATTACCC TGTTATCCCA GATGACATTA CCCTGTTATC
      481 CCTAGATACA TTACCCTGTT ATCCCAGATG ACATACCCTG TTATCCCTAG ATGACATTAC
      541 CCTGTTATCC CAGATGACAT TACCCTGTTA TCCCTAGATA CATTACCCTG TTATCCCAGA
      601 TGACATACCC TGTTATCCCT AGATGACATT ACCCTGTTAT CCCAGATGAC ATTACCCTGT
      661 TATCCCTAGA TACATTACCC TGTTATCCCA GATGACATAC CCTGTTATCC CTAGATGACA
      721 TTACCCTGTT ATCCCAGATA AACTCAATGA TGATGATGAT GATGGTCGAG ACTCAGCGGC
      781 CGCGGTGCCA GGGCGTGCCC TTGGGCTCCC CGGGCGCGAC TAGTGcctca cgaaatcgat
      841 tcgctcttCT AATCTCCTCT CTTCTCCTCT CTCCAGGGTG GCTCTGGAAG TTCAGGTGGA
      901 GGCTCGGGTG GCGGCAGTTC Ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      961 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc
     1021 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1081 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcgtggcctg cttcagccgc
     1141 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1201 caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag
     1261 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1321 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccactacgt ctatatcacg
     1381 gccgacaagc agaagaacTG Catcaaggct aacttcaaga tccgccacaa cgttgaggac
     1441 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1501 ctgctgcccg acaaccacta cctgagccat cagtccAAGc tgagcaaaga ccccaacgag
     1561 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggattac acatggcatg
     1621 gacgagctgt acaagGGATC CGGAAGTGGC TCAAGCGGAG GAGGAAGTAG TGGAAGTTCT
     1681 GTAAGTATTG GTgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1741 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1801 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1861 tggtatggct gattatgatc ctctggagat ttagccctcc cacacataac cagagggcag
     1921 caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat
     1981 cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca agatgcccct
     2041 gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag cagcagcagt
     2101 gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat tgacaccagt
     2161 gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt cagagatggg
     2221 gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt gagacaaagg
     2281 cttggccatg gtggcggatc ccgcgtcacg acacctgtgt tctggcggca aacccgttgc
     2341 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     2401 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     2461 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     2521 ctgcccactg acgggcaccg gagccTaaga gcgaatcgat ttcgtgaggC GCCCCAACTG
     2581 GGGTAACCTT TGAGTTCTCT CAGTTGGGGG TAATCAGCAT CATGATGTGG TACCACATCA
     2641 TGATGCTGAT TATAAGAATG CGGCCGCCAC ACTCTAGTGG ATCTCGAGTT AATAATTCAG
     2701 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG
     2761 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA
     2821 GCCAACGCTA TGTCCTGATA GCGGTCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA
     2881 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG
     2941 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC
     3001 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT
     3061 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA
     3121 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT
     3181 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG
     3241 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT
     3301 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG
     3361 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC
     3421 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT
     3481 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CAGAGCTTGA TCCCCTGCGC
     3541 CATCAGATCC TTGGCGGCGA GAAAGCCATC CAGTTTACTT TGCAGGGCTT CCCAACCTTA
     3601 CCAGAGGGCG CCCCAGCTGG CAATTCCGGT TCGCTTGCTG TCCATAAAAC CGCCCAGTCT
     3661 AGCTATCGCC ATGTAAGCCC ACTGCAAGCT ACCTGCTTTC TCTTTGCGCT TGCGTTTTCC
     3721 CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT CTGCGGACTG
     3781 GCTTTCTACG TGCTCGAGgg gGgccAAACG GTCTCCAGCT TGGCTGTTTT GGCGGATGAG
     3841 AGAAGATTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA
     3901 ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA
     3961 AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG
     4021 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG
     4081 TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG
     4141 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG
     4201 CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT
     4261 CTAAATACAT TCAAATATGT ATCCGCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT
     4321 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
     4381 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     4441 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
     4501 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
     4561 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
     4621 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
     4681 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
     4741 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
     4801 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
     4861 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
     4921 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
     4981 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
     5041 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA
     5101 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC
     5161 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC
     5221 CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC
     5281 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
     5341 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
     5401 CCGAAACGCG CGAGGCAGCA GATCAATTCG CGCGCGAAGG CGAAGCGGCA TGCATAATGT
     5461 GCCTGTCAAA TGGACGAAGC AGGGATTCTG CAAACCCTAT GCTACTCCGT CAAGCCGTCA
     5521 ATTGTCTGAT TCGTTACCAA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA
     5581 CAACCGGCAC GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA
     5641 TAGAGTTGAT CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG
     5701 CTCAAAAGCA GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC
     5761 CCTAACTGCT GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG
     5821 ACGCTGGCGA T    
//
